YDR233C / RTN1

Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR233C / RTN1

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:2.92|P-value:6.85E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:5.35|P-value:1.97E-9 ID:SGTC_103|Compound:0844-0104|FD-Score:3.41|P-value:9.15E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.08|P-value:3.43E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.03|P-value:4.09E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.67|P-value:1.44E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-2.86|P-value:7.96E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:2.83|P-value:9.53E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:3.40|P-value:9.48E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.90|P-value:8.53E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.22|P-value:1.87E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.09|P-value:3.50E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:4.80|P-value:6.77E-8 ID:SGTC_738|Compound:1315-0003|FD-Score:3.10|P-value:3.35E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:2.85|P-value:8.61E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.36|P-value:1.02E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:4.82|P-value:5.86E-8 ID:SGTC_878|Compound:0828-0289|FD-Score:-2.94|P-value:5.91E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.64|P-value:2.95E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.56|P-value:2.41E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.10|P-value:3.10E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.45|P-value:7.52E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.78|P-value:7.32E-8 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.10|P-value:3.27E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-3.07|P-value:3.55E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.04|P-value:4.15E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.27|P-value:1.53E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.16|P-value:2.46E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-5.99|P-value:1.97E-11 ID:SGTC_1857|Compound:st056007|FD-Score:-3.51|P-value:5.38E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-3.46|P-value:6.71E-5 ID:SGTC_1988|Compound:st071872|FD-Score:2.96|P-value:5.81E-4 ID:SGTC_2137|Compound:5261020|FD-Score:-2.84|P-value:8.69E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.20|P-value:2.19E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.86|P-value:1.05E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.58|P-value:4.20E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-2.90|P-value:6.85E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:-4.26|P-value:1.29E-6 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:2.82|P-value:9.79E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:5.11|P-value:9.75E-9 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:9.60|P-value:2.16E-26 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.11|P-value:3.08E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:2.95|P-value:6.01E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.66|P-value:2.90E-5 ID:SGTC_2855|Compound:9016525|FD-Score:3.03|P-value:4.41E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.80|P-value:1.46E-5 ID:SGTC_2877|Compound:9040724|FD-Score:3.12|P-value:3.07E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.10|P-value:3.10E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.74|P-value:1.88E-5 ID:SGTC_3049|Compound:9092223|FD-Score:2.87|P-value:8.27E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-2.95|P-value:5.62E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-5.05|P-value:1.26E-8 ID:SGTC_3153|Compound:9100258|FD-Score:3.17|P-value:2.52E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:2.92|P-value:6.85E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:5.35|P-value:1.97E-9 ID:SGTC_103|Compound:0844-0104|FD-Score:3.41|P-value:9.15E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.08|P-value:3.43E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.03|P-value:4.09E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.67|P-value:1.44E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-2.86|P-value:7.96E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:2.83|P-value:9.53E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:3.40|P-value:9.48E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.90|P-value:8.53E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.22|P-value:1.87E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.09|P-value:3.50E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:4.80|P-value:6.77E-8 ID:SGTC_738|Compound:1315-0003|FD-Score:3.10|P-value:3.35E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:2.85|P-value:8.61E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.36|P-value:1.02E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:4.82|P-value:5.86E-8 ID:SGTC_878|Compound:0828-0289|FD-Score:-2.94|P-value:5.91E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.64|P-value:2.95E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.56|P-value:2.41E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.10|P-value:3.10E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.45|P-value:7.52E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.78|P-value:7.32E-8 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.10|P-value:3.27E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-3.07|P-value:3.55E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.04|P-value:4.15E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.27|P-value:1.53E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.16|P-value:2.46E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-5.99|P-value:1.97E-11 ID:SGTC_1857|Compound:st056007|FD-Score:-3.51|P-value:5.38E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-3.46|P-value:6.71E-5 ID:SGTC_1988|Compound:st071872|FD-Score:2.96|P-value:5.81E-4 ID:SGTC_2137|Compound:5261020|FD-Score:-2.84|P-value:8.69E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.20|P-value:2.19E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.86|P-value:1.05E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.58|P-value:4.20E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-2.90|P-value:6.85E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:-4.26|P-value:1.29E-6 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:2.82|P-value:9.79E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:5.11|P-value:9.75E-9 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:9.60|P-value:2.16E-26 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.11|P-value:3.08E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:2.95|P-value:6.01E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.66|P-value:2.90E-5 ID:SGTC_2855|Compound:9016525|FD-Score:3.03|P-value:4.41E-4 ID:SGTC_2874|Compound:9043904|FD-Score:3.80|P-value:1.46E-5 ID:SGTC_2877|Compound:9040724|FD-Score:3.12|P-value:3.07E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.10|P-value:3.10E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.74|P-value:1.88E-5 ID:SGTC_3049|Compound:9092223|FD-Score:2.87|P-value:8.27E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-2.95|P-value:5.62E-4 ID:SGTC_3077|Compound:9117319|FD-Score:-5.05|P-value:1.26E-8 ID:SGTC_3153|Compound:9100258|FD-Score:3.17|P-value:2.52E-4

Top fitness defect scores for YDR233C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2633 5,7-dihydroxyisoflavone 9.60 100.0 µM 2.16E-26 1 3.86E-22
2 SGTC_34 0469-0683 5.35 81.8 µM 1.97E-9 SWF1 & branched chain AA biosynthesis 12 4.29E-8
3 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 5.11 100.0 µM 9.75E-9 16 1.62E-7
4 SGTC_875 0709-0210 4.82 25.7 µM 5.86E-8 fatty acid desaturase (OLE1) 20 7.18E-7
5 SGTC_695 3505-6186 4.80 243.0 µM 6.77E-8 12 8.11E-7
6 SGTC_1205 4161-2785 4.78 76.0 µM 7.32E-8 14 8.65E-7
7 SGTC_137 0438-0332 4.67 85.4 µM 1.44E-7 ubiquinone biosynthesis & proteasome 89 1.52E-6
8 SGTC_2636 apigenin dimethyl ether 4.11 100.0 µM 3.08E-6 40 1.94E-5
9 SGTC_2874 9043904 3.80 71.4 µM 1.46E-5 17 7.11E-5
10 SGTC_2766 oltipraz 3.66 83.9 µM 2.90E-5 heme requiring 36 1.26E-4
11 SGTC_2541 muurolladie-3-one 3.58 83.1 µM 4.20E-5 excess fatty acid 44 1.72E-4
12 SGTC_1026 k007-1105 3.45 30.6 µM 7.52E-5 ERAD & cell cycle 122 2.80E-4
13 SGTC_103 0844-0104 3.41 51.8 µM 9.15E-5 ERAD & cell cycle 174 3.29E-4
14 SGTC_405 ebelactone b 3.40 50.0 µM 9.48E-5 36 3.39E-4
15 SGTC_2409 alverine citrate 3.20 64.0 µM 2.19E-4 87 6.83E-4
16 SGTC_3153 9100258 3.17 49.5 µM 2.52E-4 61 7.68E-4
17 SGTC_2877 9040724 3.12 71.4 µM 3.07E-4 45 9.06E-4
18 SGTC_1263 0774-1208 3.10 64.8 µM 3.27E-4 100 9.57E-4
19 SGTC_738 1315-0003 3.10 72.4 µM 3.35E-4 54 9.77E-4
20 SGTC_515 4092-1064 3.09 102.0 µM 3.50E-4 plasma membrane duress 101 0.00101

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.136 2.51E-15 YDL190C UFD2 hom Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
0.124 6.47E-13 YLR300W EXG1 hom Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes
0.124 6.68E-13 YNL338W_d YNL338W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV
0.116 1.52E-11 YBR230C OM14 hom Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron
0.115 2.02E-11 YDR442W_d YDR442W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.110 2.04E-10 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.106 7.28E-10 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.103 2.57E-9 YEL043W YEL043W hom Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
0.099 7.90E-9 YOL164W-A_p YOL164W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.096 2.81E-8 YNL045W LAP2 hom Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.094 4.79E-8 YMR305C SCW10 hom Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p
0.094 5.12E-8 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.091 1.39E-7 YNL090W RHO2 hom Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
0.089 2.08E-7 YDR126W SWF1 hom Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
0.088 3.65E-7 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p