YDR243C / PRP28

RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR243C / PRP28

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:-3.14|P-value:5.03E-4 ID:SGTC_76|Compound:0433-0009|FD-Score:3.80|P-value:4.17E-5 ID:SGTC_85|Compound:0868-0259|FD-Score:3.35|P-value:2.59E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.53|P-value:1.12E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:3.38|P-value:2.31E-4 ID:SGTC_229|Compound:benomyl|FD-Score:5.11|P-value:5.76E-8 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.28|P-value:3.37E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.31|P-value:3.08E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.57|P-value:1.07E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:4.06|P-value:1.26E-5 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.04|P-value:8.36E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:-3.46|P-value:1.48E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.35|P-value:2.56E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:-3.80|P-value:3.54E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:-2.98|P-value:8.94E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.23|P-value:3.64E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.08|P-value:7.06E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:5.29|P-value:1.97E-8 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.98|P-value:1.88E-5 ID:SGTC_1642|Compound:st009582|FD-Score:3.21|P-value:4.42E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.42|P-value:1.96E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-3.25|P-value:3.31E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.39|P-value:2.23E-4 ID:SGTC_2028|Compound:5153890|FD-Score:-3.94|P-value:1.89E-5 ID:SGTC_2038|Compound:4100038|FD-Score:3.00|P-value:9.49E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.05|P-value:7.96E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.04|P-value:8.25E-4 ID:SGTC_2303|Compound:7774051|FD-Score:4.20|P-value:6.74E-6 ID:SGTC_2327|Compound:6189656|FD-Score:3.74|P-value:5.22E-5 ID:SGTC_2517|Compound:totarol|FD-Score:-3.56|P-value:9.63E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.25|P-value:3.87E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_2814|Compound:7977482|FD-Score:-2.96|P-value:9.85E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.02|P-value:8.77E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-4.94|P-value:1.25E-7 ID:SGTC_2840|Compound:9008771|FD-Score:5.56|P-value:4.01E-9 ID:SGTC_2845|Compound:9003024|FD-Score:3.26|P-value:3.73E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.33|P-value:2.80E-4 ID:SGTC_2977|Compound:9011197|FD-Score:3.50|P-value:1.45E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.04|P-value:8.08E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.10|P-value:6.54E-4 ID:SGTC_3170|Compound:9103054|FD-Score:5.34|P-value:1.51E-8 ID:SGTC_3180|Compound:9109287|FD-Score:-3.09|P-value:5.99E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:-3.14|P-value:5.03E-4 ID:SGTC_76|Compound:0433-0009|FD-Score:3.80|P-value:4.17E-5 ID:SGTC_85|Compound:0868-0259|FD-Score:3.35|P-value:2.59E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.53|P-value:1.12E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:3.38|P-value:2.31E-4 ID:SGTC_229|Compound:benomyl|FD-Score:5.11|P-value:5.76E-8 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.28|P-value:3.37E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.31|P-value:3.08E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.57|P-value:1.07E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:4.06|P-value:1.26E-5 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.04|P-value:8.36E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:-3.46|P-value:1.48E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.35|P-value:2.56E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:-3.80|P-value:3.54E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:-2.98|P-value:8.94E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.23|P-value:3.64E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.08|P-value:7.06E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:5.29|P-value:1.97E-8 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.98|P-value:1.88E-5 ID:SGTC_1642|Compound:st009582|FD-Score:3.21|P-value:4.42E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.42|P-value:1.96E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-3.25|P-value:3.31E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.39|P-value:2.23E-4 ID:SGTC_2028|Compound:5153890|FD-Score:-3.94|P-value:1.89E-5 ID:SGTC_2038|Compound:4100038|FD-Score:3.00|P-value:9.49E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.05|P-value:7.96E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.04|P-value:8.25E-4 ID:SGTC_2303|Compound:7774051|FD-Score:4.20|P-value:6.74E-6 ID:SGTC_2327|Compound:6189656|FD-Score:3.74|P-value:5.22E-5 ID:SGTC_2517|Compound:totarol|FD-Score:-3.56|P-value:9.63E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.25|P-value:3.87E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_2814|Compound:7977482|FD-Score:-2.96|P-value:9.85E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.02|P-value:8.77E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-4.94|P-value:1.25E-7 ID:SGTC_2840|Compound:9008771|FD-Score:5.56|P-value:4.01E-9 ID:SGTC_2845|Compound:9003024|FD-Score:3.26|P-value:3.73E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.33|P-value:2.80E-4 ID:SGTC_2977|Compound:9011197|FD-Score:3.50|P-value:1.45E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.04|P-value:8.08E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.10|P-value:6.54E-4 ID:SGTC_3170|Compound:9103054|FD-Score:5.34|P-value:1.51E-8 ID:SGTC_3180|Compound:9109287|FD-Score:-3.09|P-value:5.99E-4

Top fitness defect scores for YDR243C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2840 9008771 5.56 71.4 µM 4.01E-9 1 1.38E-8 2.13
2 SGTC_3170 9103054 5.34 49.5 µM 1.51E-8 60S ribosome export 4 4.71E-8 0.98
3 SGTC_1584 6-fluoro-dl-tryptophan 5.29 90.0 µM 1.97E-8 mitochondrial processes 4 6.03E-8 0.35
4 SGTC_229 benomyl 5.11 22.9 µM 5.76E-8 tubulin folding & SWR complex 12 1.63E-7 1.39
5 SGTC_2303 7774051 4.20 200.0 µM 6.74E-6 3 1.36E-5 0.18
6 SGTC_941 3237-0776 4.06 460.0 µM 1.26E-5 4 2.43E-5 0.30
7 SGTC_1599 8-chlro-1-tetrahydronorharmanone 3.98 90.6 µM 1.88E-5 9 3.51E-5 0.34
8 SGTC_76 0433-0009 3.80 400.3 µM 4.17E-5 9 7.37E-5 0.53
9 SGTC_2327 6189656 3.74 200.0 µM 5.22E-5 9 9.08E-5 0.08
10 SGTC_629 3448-6466 3.57 76.7 µM 1.07E-4 5 1.77E-4 0.20
11 SGTC_2977 9011197 3.50 71.4 µM 1.45E-4 14 2.35E-4 0.19
12 SGTC_2768 sulfasalazine 3.49 50.2 µM 1.51E-4 17 2.45E-4 0.05
13 SGTC_1645 Cervolide 3.42 78.0 µM 1.96E-4 7 3.12E-4 0.19
14 SGTC_1919 st067113 3.39 52.3 µM 2.23E-4 TSC3-RPN4 14 3.51E-4 0.04
15 SGTC_135 0933-0004 3.38 26.4 µM 2.31E-4 60S ribosome export 12 3.63E-4 0.16
16 SGTC_1340 1486-1359 3.35 257.0 µM 2.56E-4 9 3.98E-4 0.01
17 SGTC_85 0868-0259 3.35 160.1 µM 2.59E-4 60S ribosome export 12 4.04E-4 0.15
18 SGTC_2919 7978188 3.33 46.4 µM 2.80E-4 8 4.34E-4 0.11
19 SGTC_507 nimesulide 3.31 162.0 µM 3.08E-4 7 4.75E-4 0.04
20 SGTC_436 n,n-dimethylsphingosine 3.28 3.3 µM 3.37E-4 8 5.15E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.163 2.19E-21 YER033C ZRG8 hom Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
0.107 5.36E-10 YDR251W PAM1 hom Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype
0.106 8.80E-10 YDL153C SAS10 het Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
0.103 2.10E-9 YPL103C FMP30 hom Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
0.102 2.97E-9 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
0.096 2.75E-8 YLR195C NMT1 het N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.095 4.20E-8 YHR019C DED81 het Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
0.094 5.33E-8 YBR185C MBA1 hom Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane
0.094 5.47E-8 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.085 9.01E-7 YDR247W VHS1 hom Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p
0.083 1.73E-6 YPR195C_d YPR195C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.078 5.43E-6 YOL012C HTZ1 hom Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
0.077 8.07E-6 YLR134W PDC5 hom Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
0.076 1.00E-5 YLR184W_d YLR184W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.076 1.16E-5 YGL248W PDE1 hom Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation