YDR245W / MNN10

Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR245W / MNN10

Click on Significant Values for Screen Details ID:SGTC_14|Compound:0354-0009|FD-Score:3.28|P-value:9.23E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:3.31|P-value:8.40E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:5.01|P-value:1.07E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:4.10|P-value:5.05E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:3.60|P-value:3.22E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.62|P-value:6.16E-6 ID:SGTC_206|Compound:4546-0025|FD-Score:4.21|P-value:3.26E-5 ID:SGTC_472|Compound:calcifediol|FD-Score:-3.46|P-value:6.25E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:5.69|P-value:3.75E-8 ID:SGTC_520|Compound:3584-0023|FD-Score:3.64|P-value:2.81E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:5.42|P-value:1.46E-7 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.87|P-value:1.49E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.01|P-value:7.20E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:8.59|P-value:2.56E-16 ID:SGTC_586|Compound:0957-0265|FD-Score:-3.95|P-value:1.13E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:-3.81|P-value:1.84E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:6.17|P-value:2.70E-9 ID:SGTC_663|Compound:1486-0733|FD-Score:5.89|P-value:1.30E-8 ID:SGTC_701|Compound:4424-0219|FD-Score:3.40|P-value:6.30E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.86|P-value:1.27E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.28|P-value:2.98E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.33|P-value:7.79E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:6.31|P-value:1.18E-9 ID:SGTC_913|Compound:3487-1245|FD-Score:3.47|P-value:5.04E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:3.33|P-value:7.85E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.87|P-value:1.21E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:4.28|P-value:2.50E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-3.88|P-value:1.43E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.32|P-value:8.12E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:4.88|P-value:1.90E-6 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.48|P-value:4.72E-4 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.53|P-value:4.08E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.82|P-value:1.48E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.48|P-value:4.82E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.39|P-value:6.48E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.74|P-value:1.97E-4 ID:SGTC_1738|Compound:st035555|FD-Score:4.39|P-value:1.60E-5 ID:SGTC_1797|Compound:st048695|FD-Score:5.10|P-value:6.87E-7 ID:SGTC_2007|Compound:4011539|FD-Score:4.05|P-value:6.22E-5 ID:SGTC_2013|Compound:4024628|FD-Score:3.93|P-value:9.56E-5 ID:SGTC_2306|Compound:7482397|FD-Score:3.47|P-value:5.00E-4 ID:SGTC_2373|Compound:9071805|FD-Score:3.68|P-value:2.40E-4 ID:SGTC_2399|Compound:5884133|FD-Score:7.04|P-value:1.42E-11 ID:SGTC_2414|Compound:st077232|FD-Score:4.45|P-value:1.24E-5 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.61|P-value:6.44E-6 ID:SGTC_2556|Compound:citropten|FD-Score:4.80|P-value:2.71E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:5.75|P-value:2.69E-8 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.27|P-value:2.65E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.90|P-value:1.09E-4 ID:SGTC_2780|Compound:5215298|FD-Score:5.04|P-value:9.39E-7 ID:SGTC_2786|Compound:7743617|FD-Score:6.33|P-value:1.07E-9 ID:SGTC_2939|Compound:9044578|FD-Score:3.56|P-value:3.66E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.70|P-value:2.23E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-4.81|P-value:3.35E-6 ID:SGTC_3132|Compound:9126706|FD-Score:3.84|P-value:1.35E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.28|P-value:9.18E-4 ID:SGTC_3298|Compound:9116858|FD-Score:-4.69|P-value:5.60E-6 ID:SGTC_3311|Compound:9131146|FD-Score:3.89|P-value:1.11E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:3.28|P-value:9.23E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:3.31|P-value:8.40E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:5.01|P-value:1.07E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:4.10|P-value:5.05E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:3.60|P-value:3.22E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.62|P-value:6.16E-6 ID:SGTC_206|Compound:4546-0025|FD-Score:4.21|P-value:3.26E-5 ID:SGTC_472|Compound:calcifediol|FD-Score:-3.46|P-value:6.25E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:5.69|P-value:3.75E-8 ID:SGTC_520|Compound:3584-0023|FD-Score:3.64|P-value:2.81E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:5.42|P-value:1.46E-7 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.87|P-value:1.49E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.01|P-value:7.20E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:8.59|P-value:2.56E-16 ID:SGTC_586|Compound:0957-0265|FD-Score:-3.95|P-value:1.13E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:-3.81|P-value:1.84E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:6.17|P-value:2.70E-9 ID:SGTC_663|Compound:1486-0733|FD-Score:5.89|P-value:1.30E-8 ID:SGTC_701|Compound:4424-0219|FD-Score:3.40|P-value:6.30E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.86|P-value:1.27E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:5.28|P-value:2.98E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.33|P-value:7.79E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:6.31|P-value:1.18E-9 ID:SGTC_913|Compound:3487-1245|FD-Score:3.47|P-value:5.04E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:3.33|P-value:7.85E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.87|P-value:1.21E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:4.28|P-value:2.50E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-3.88|P-value:1.43E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.32|P-value:8.12E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:4.88|P-value:1.90E-6 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.48|P-value:4.72E-4 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.53|P-value:4.08E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.82|P-value:1.48E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.48|P-value:4.82E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.39|P-value:6.48E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.74|P-value:1.97E-4 ID:SGTC_1738|Compound:st035555|FD-Score:4.39|P-value:1.60E-5 ID:SGTC_1797|Compound:st048695|FD-Score:5.10|P-value:6.87E-7 ID:SGTC_2007|Compound:4011539|FD-Score:4.05|P-value:6.22E-5 ID:SGTC_2013|Compound:4024628|FD-Score:3.93|P-value:9.56E-5 ID:SGTC_2306|Compound:7482397|FD-Score:3.47|P-value:5.00E-4 ID:SGTC_2373|Compound:9071805|FD-Score:3.68|P-value:2.40E-4 ID:SGTC_2399|Compound:5884133|FD-Score:7.04|P-value:1.42E-11 ID:SGTC_2414|Compound:st077232|FD-Score:4.45|P-value:1.24E-5 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.61|P-value:6.44E-6 ID:SGTC_2556|Compound:citropten|FD-Score:4.80|P-value:2.71E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:5.75|P-value:2.69E-8 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.27|P-value:2.65E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.90|P-value:1.09E-4 ID:SGTC_2780|Compound:5215298|FD-Score:5.04|P-value:9.39E-7 ID:SGTC_2786|Compound:7743617|FD-Score:6.33|P-value:1.07E-9 ID:SGTC_2939|Compound:9044578|FD-Score:3.56|P-value:3.66E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.70|P-value:2.23E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-4.81|P-value:3.35E-6 ID:SGTC_3132|Compound:9126706|FD-Score:3.84|P-value:1.35E-4 ID:SGTC_3207|Compound:9114163|FD-Score:3.28|P-value:9.18E-4 ID:SGTC_3298|Compound:9116858|FD-Score:-4.69|P-value:5.60E-6 ID:SGTC_3311|Compound:9131146|FD-Score:3.89|P-value:1.11E-4

Top fitness defect scores for YDR245W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_581 0958-0271 8.59 211.0 µM 2.56E-16 5 4.51E-18
2 SGTC_2399 5884133 7.04 200.0 µM 1.42E-11 cell wall signaling 21 9.53E-13
3 SGTC_2786 7743617 6.33 45.5 µM 1.07E-9 2 1.21E-10
4 SGTC_908 3448-7491 6.31 47.7 µM 1.18E-9 mitochondrial stress 39 1.36E-10
5 SGTC_625 k213-0058 6.17 121.0 µM 2.70E-9 translation 31 3.41E-10
6 SGTC_663 1486-0733 5.89 13.9 µM 1.30E-8 cell wall signaling 20 1.98E-9
7 SGTC_2594 a-mangostin 5.75 100.0 µM 2.69E-8 12 4.49E-9
8 SGTC_517 1365-0151 5.69 113.0 µM 3.75E-8 12 6.51E-9
9 SGTC_565 1418-0001 5.42 44.5 µM 1.46E-7 3 2.97E-8
10 SGTC_830 4076-0286 5.28 57.8 µM 2.98E-7 translation 26 6.63E-8
11 SGTC_1797 st048695 5.10 56.8 µM 6.87E-7 NEO1 53 1.68E-7
12 SGTC_2780 5215298 5.04 71.4 µM 9.39E-7 14 2.39E-7
13 SGTC_50 0929-0078 5.01 56.4 µM 1.07E-6 29 2.77E-7
14 SGTC_1104 tacrolimus 4.88 29.8 µM 1.90E-6 22 5.25E-7
15 SGTC_2556 citropten 4.80 84.6 µM 2.71E-6 13 7.80E-7
16 SGTC_131 0141-0277 4.62 33.9 µM 6.16E-6 23 1.95E-6
17 SGTC_2536 dehydroabietamide 4.61 32.0 µM 6.44E-6 13 2.06E-6
18 SGTC_2414 st077232 4.45 92.9 µM 1.24E-5 15 4.26E-6
19 SGTC_1738 st035555 4.39 74.0 µM 1.60E-5 TSC3-RPN4 21 5.66E-6
20 SGTC_1077 5-fluorocytosine 4.28 377.0 nM 2.50E-5 exosome 11 9.33E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.229 2.67E-41 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.200 1.02E-31 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.194 1.19E-29 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.183 9.12E-27 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.182 2.64E-26 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.167 1.83E-22 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.163 2.25E-21 YJL183W MNN11 hom Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.160 1.54E-20 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.157 5.78E-20 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.148 6.75E-18 YJR075W HOC1 hom Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.142 1.44E-16 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.141 2.66E-16 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.140 4.66E-16 YNL084C END3 hom EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
0.136 3.24E-15 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.130 5.02E-14 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching