YDR250C_d

Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR250C / YDR250C

Click on Significant Values for Screen Details ID:SGTC_131|Compound:0141-0277|FD-Score:4.43|P-value:5.24E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:3.27|P-value:5.77E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.60|P-value:1.68E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.17|P-value:8.00E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.52|P-value:2.32E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:4.61|P-value:2.29E-6 ID:SGTC_803|Compound:4488-1585|FD-Score:3.27|P-value:5.73E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.26|P-value:5.94E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-4.09|P-value:2.37E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.21|P-value:7.10E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.53|P-value:3.22E-6 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.23|P-value:6.48E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:4.37|P-value:7.01E-6 ID:SGTC_1731|Compound:st035964|FD-Score:3.13|P-value:9.22E-4 ID:SGTC_1757|Compound:st041620|FD-Score:3.83|P-value:7.04E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.30|P-value:5.22E-4 ID:SGTC_2277|Compound:7947813|FD-Score:4.50|P-value:3.69E-6 ID:SGTC_2369|Compound:9071472|FD-Score:3.36|P-value:4.17E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.43|P-value:3.24E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-4.29|P-value:9.59E-6 ID:SGTC_2625|Compound:euphol|FD-Score:4.64|P-value:1.95E-6 ID:SGTC_2811|Compound:7967822|FD-Score:-3.57|P-value:1.90E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.26|P-value:5.82E-4 ID:SGTC_2840|Compound:9008771|FD-Score:3.37|P-value:4.04E-4 ID:SGTC_2843|Compound:9000894|FD-Score:4.32|P-value:8.45E-6 ID:SGTC_3016|Compound:9080866|FD-Score:4.21|P-value:1.38E-5 ID:SGTC_3046|Compound:9092212|FD-Score:-3.12|P-value:9.59E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.18|P-value:7.72E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.43|P-value:5.24E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:3.27|P-value:5.77E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.60|P-value:1.68E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.17|P-value:8.00E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.52|P-value:2.32E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:4.61|P-value:2.29E-6 ID:SGTC_803|Compound:4488-1585|FD-Score:3.27|P-value:5.73E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.26|P-value:5.94E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-4.09|P-value:2.37E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.21|P-value:7.10E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.53|P-value:3.22E-6 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.23|P-value:6.48E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:4.37|P-value:7.01E-6 ID:SGTC_1731|Compound:st035964|FD-Score:3.13|P-value:9.22E-4 ID:SGTC_1757|Compound:st041620|FD-Score:3.83|P-value:7.04E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.30|P-value:5.22E-4 ID:SGTC_2277|Compound:7947813|FD-Score:4.50|P-value:3.69E-6 ID:SGTC_2369|Compound:9071472|FD-Score:3.36|P-value:4.17E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.43|P-value:3.24E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-4.29|P-value:9.59E-6 ID:SGTC_2625|Compound:euphol|FD-Score:4.64|P-value:1.95E-6 ID:SGTC_2811|Compound:7967822|FD-Score:-3.57|P-value:1.90E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.26|P-value:5.82E-4 ID:SGTC_2840|Compound:9008771|FD-Score:3.37|P-value:4.04E-4 ID:SGTC_2843|Compound:9000894|FD-Score:4.32|P-value:8.45E-6 ID:SGTC_3016|Compound:9080866|FD-Score:4.21|P-value:1.38E-5 ID:SGTC_3046|Compound:9092212|FD-Score:-3.12|P-value:9.59E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.18|P-value:7.72E-4

Top fitness defect scores for YDR250C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2625 euphol 4.64 100.0 µM 1.95E-6 20 1.74E-6
2 SGTC_784 4579-0127 4.61 173.0 µM 2.29E-6 9 2.05E-6
3 SGTC_1250 0485-0349 4.53 40.2 µM 3.22E-6 18 2.89E-6
4 SGTC_2277 7947813 4.50 106.9 µM 3.69E-6 RSC complex & mRNA processing 15 3.32E-6
5 SGTC_131 0141-0277 4.43 33.9 µM 5.24E-6 27 4.72E-6
6 SGTC_1493 4348-0038 4.37 111.0 µM 7.01E-6 RSC complex & mRNA processing 16 6.34E-6
7 SGTC_2843 9000894 4.32 45.5 µM 8.45E-6 9 7.66E-6
8 SGTC_3016 9080866 4.21 49.5 µM 1.38E-5 22 1.25E-5
9 SGTC_1757 st041620 3.83 31.8 µM 7.04E-5 34 6.51E-5
10 SGTC_264 promethazine 3.60 12.5 mM 1.68E-4 DNA intercalators 64 1.57E-4
11 SGTC_780 0109-0199 3.52 192.0 µM 2.32E-4 30 2.17E-4
12 SGTC_2840 9008771 3.37 71.4 µM 4.04E-4 19 3.80E-4
13 SGTC_2369 9071472 3.36 200.0 µM 4.17E-4 32 3.92E-4
14 SGTC_1926 methyl fluorone black 3.30 53.6 µM 5.22E-4 DNA intercalators 87 4.92E-4
15 SGTC_803 4488-1585 3.27 147.0 µM 5.73E-4 33 5.40E-4
16 SGTC_184 1494-0393 3.27 24.7 µM 5.77E-4 97 5.44E-4
17 SGTC_2812 7970281 3.26 58.4 µM 5.82E-4 65 5.49E-4
18 SGTC_1039 k821-0043 3.26 94.0 µM 5.94E-4 Golgi 52 5.61E-4
19 SGTC_1248 0469-0687 3.21 58.6 µM 7.10E-4 63 6.71E-4
20 SGTC_1731 st035964 3.13 63.0 µM 9.22E-4 69 8.73E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.184 5.92E-27 YER099C PRS2 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
0.156 1.20E-19 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.149 4.31E-18 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.147 1.49E-17 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.129 7.29E-14 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.122 1.46E-12 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.118 8.61E-12 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.114 3.22E-11 YNL269W BSC4 hom Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p
0.114 4.37E-11 YPL035C_d YPL035C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
0.113 4.52E-11 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.111 1.00E-10 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.109 2.56E-10 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.108 3.16E-10 YGL243W TAD1 hom tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala
0.107 4.58E-10 YDR314C RAD34 hom Protein involved in nucleotide excision repair (NER); homologous to RAD4
0.103 1.91E-9 YEL006W YEA6 hom Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog