YDR251W / PAM1

Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR251W / PAM1

Click on Significant Values for Screen Details ID:SGTC_13|Compound:0199-0087|FD-Score:4.31|P-value:2.16E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.76|P-value:3.16E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:3.14|P-value:4.24E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.78|P-value:2.85E-5 ID:SGTC_229|Compound:benomyl|FD-Score:3.38|P-value:1.61E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.46|P-value:1.14E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.20|P-value:3.31E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.09|P-value:5.02E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.46|P-value:1.18E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.27|P-value:2.52E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:5.42|P-value:3.45E-9 ID:SGTC_941|Compound:3237-0776|FD-Score:3.04|P-value:6.18E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:-2.98|P-value:5.85E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:3.84|P-value:2.15E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:-4.03|P-value:5.93E-6 ID:SGTC_982|Compound:1491-0518|FD-Score:-3.12|P-value:3.47E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:-2.90|P-value:8.01E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:2.98|P-value:7.62E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.42|P-value:1.20E-6 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.71|P-value:3.89E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.11|P-value:3.53E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.30|P-value:1.63E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.41|P-value:1.41E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:-3.01|P-value:5.29E-4 ID:SGTC_1637|Compound:st007924|FD-Score:2.98|P-value:7.85E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-3.10|P-value:3.66E-4 ID:SGTC_1730|Compound:st033808|FD-Score:2.93|P-value:9.20E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.41|P-value:1.41E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.15|P-value:4.11E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.18|P-value:3.67E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.99|P-value:7.43E-4 ID:SGTC_2186|Compound:6124449|FD-Score:3.27|P-value:2.55E-4 ID:SGTC_2390|Compound:9097792|FD-Score:3.04|P-value:6.22E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.46|P-value:8.34E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.07|P-value:5.49E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:2.99|P-value:7.41E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.20|P-value:3.33E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-2.99|P-value:5.69E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:2.96|P-value:8.40E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:4.06|P-value:7.61E-6 ID:SGTC_2760|Compound:etidronate|FD-Score:3.63|P-value:5.48E-5 ID:SGTC_2829|Compound:7992743|FD-Score:3.23|P-value:2.93E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-7.52|P-value:2.17E-16 ID:SGTC_2864|Compound:9034755|FD-Score:-3.85|P-value:1.47E-5 ID:SGTC_2873|Compound:9040596|FD-Score:4.52|P-value:6.95E-7 ID:SGTC_2874|Compound:9043904|FD-Score:-2.85|P-value:9.31E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.72|P-value:3.66E-5 ID:SGTC_2992|Compound:9074591|FD-Score:-3.44|P-value:9.16E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.26|P-value:1.92E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.31|P-value:1.59E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.03|P-value:6.39E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:4.31|P-value:2.16E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.76|P-value:3.16E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:3.14|P-value:4.24E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.78|P-value:2.85E-5 ID:SGTC_229|Compound:benomyl|FD-Score:3.38|P-value:1.61E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.46|P-value:1.14E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.20|P-value:3.31E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.09|P-value:5.02E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.46|P-value:1.18E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.27|P-value:2.52E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:5.42|P-value:3.45E-9 ID:SGTC_941|Compound:3237-0776|FD-Score:3.04|P-value:6.18E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:-2.98|P-value:5.85E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:3.84|P-value:2.15E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:-4.03|P-value:5.93E-6 ID:SGTC_982|Compound:1491-0518|FD-Score:-3.12|P-value:3.47E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:-2.90|P-value:8.01E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:2.98|P-value:7.62E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.42|P-value:1.20E-6 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.71|P-value:3.89E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.11|P-value:3.53E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.30|P-value:1.63E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.41|P-value:1.41E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:-3.01|P-value:5.29E-4 ID:SGTC_1637|Compound:st007924|FD-Score:2.98|P-value:7.85E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-3.10|P-value:3.66E-4 ID:SGTC_1730|Compound:st033808|FD-Score:2.93|P-value:9.20E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.41|P-value:1.41E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.15|P-value:4.11E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.18|P-value:3.67E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.99|P-value:7.43E-4 ID:SGTC_2186|Compound:6124449|FD-Score:3.27|P-value:2.55E-4 ID:SGTC_2390|Compound:9097792|FD-Score:3.04|P-value:6.22E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.46|P-value:8.34E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.07|P-value:5.49E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:2.99|P-value:7.41E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.20|P-value:3.33E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-2.99|P-value:5.69E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:2.96|P-value:8.40E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:4.06|P-value:7.61E-6 ID:SGTC_2760|Compound:etidronate|FD-Score:3.63|P-value:5.48E-5 ID:SGTC_2829|Compound:7992743|FD-Score:3.23|P-value:2.93E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-7.52|P-value:2.17E-16 ID:SGTC_2864|Compound:9034755|FD-Score:-3.85|P-value:1.47E-5 ID:SGTC_2873|Compound:9040596|FD-Score:4.52|P-value:6.95E-7 ID:SGTC_2874|Compound:9043904|FD-Score:-2.85|P-value:9.31E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.72|P-value:3.66E-5 ID:SGTC_2992|Compound:9074591|FD-Score:-3.44|P-value:9.16E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.26|P-value:1.92E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.31|P-value:1.59E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.03|P-value:6.39E-4

Top fitness defect scores for YDR251W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_633 0437-0093 5.42 97.7 µM 3.45E-9 DNA intercalators 4 2.94E-8
2 SGTC_2873 9040596 4.52 71.4 µM 6.95E-7 32 3.04E-6
3 SGTC_1033 k072-0102 4.42 21.3 µM 1.20E-6 60S ribosome export 12 4.89E-6
4 SGTC_13 0199-0087 4.31 65.0 µM 2.16E-6 ERG2 7 8.19E-6
5 SGTC_2756 carbadox 4.06 75.1 µM 7.61E-6 54 2.48E-5
6 SGTC_956 1185-0240 3.84 191.0 µM 2.15E-5 27 6.16E-5
7 SGTC_212 3807-4682 3.78 38.8 µM 2.85E-5 tubulin folding & SWR complex 76 7.88E-5
8 SGTC_45 0kpi-0036 3.76 14.9 µM 3.16E-5 excess fatty acid 32 8.63E-5
9 SGTC_2991 9074669 3.72 71.4 µM 3.66E-5 azole & statin 76 9.81E-5
10 SGTC_1034 k072-0165 3.71 12.7 µM 3.89E-5 Golgi 26 1.04E-4
11 SGTC_2760 etidronate 3.63 98.0 µM 5.48E-5 38 1.40E-4
12 SGTC_249 wiskostatin 3.46 11.2 µM 1.14E-4 27 2.67E-4
13 SGTC_402 epigallocatechin gallate 3.46 10.0 µM 1.18E-4 50 2.74E-4
14 SGTC_1843 st055809 3.41 45.5 µM 1.41E-4 33 3.22E-4
15 SGTC_1406 3909-7906 3.41 11.9 µM 1.41E-4 60S ribosome export 22 3.22E-4
16 SGTC_229 benomyl 3.38 22.9 µM 1.61E-4 tubulin folding & SWR complex 45 3.61E-4
17 SGTC_621 3584-0052 3.27 32.1 µM 2.52E-4 44 5.36E-4
18 SGTC_2186 6124449 3.27 200.0 µM 2.55E-4 RSC complex & mRNA processing 43 5.41E-4
19 SGTC_2829 7992743 3.23 58.4 µM 2.93E-4 70 6.12E-4
20 SGTC_263 alizarin 3.20 107.9 µM 3.31E-4 iron homeostasis 108 6.83E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 3.26E-14 YDR182W CDC1 het Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
0.107 5.36E-10 YDR243C PRP28 het RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
0.106 8.16E-10 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.104 1.56E-9 YMR313C TGL3 hom Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation
0.102 2.79E-9 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.100 6.16E-9 YAL054C ACS1 hom Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
0.093 6.92E-8 YDR318W MCM21 hom Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
0.090 1.64E-7 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.088 2.96E-7 YDL241W_p YDL241W_p hom Putative protein of unknown function; YDL241W is not an essential gene
0.087 3.89E-7 YDR262W_p YDR262W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment
0.086 7.04E-7 YGL065C ALG2 het Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
0.082 1.92E-6 YDR327W_d YDR327W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
0.080 3.39E-6 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
0.080 3.44E-6 YGR161W-C_p YGR161W-C_p hom Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
0.078 5.45E-6 YDR487C RIB3 het 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration