YDR253C / MET32

Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR253C / MET32

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:9.76|P-value:1.06E-20 ID:SGTC_138|Compound:0850-0259|FD-Score:6.28|P-value:1.39E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:7.00|P-value:1.69E-11 ID:SGTC_146|Compound:3448-8650|FD-Score:5.32|P-value:2.44E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-5.12|P-value:4.44E-7 ID:SGTC_155|Compound:k072-0202|FD-Score:3.54|P-value:4.33E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.27|P-value:7.93E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:3.35|P-value:8.19E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.94|P-value:1.02E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:7.46|P-value:7.68E-13 ID:SGTC_532|Compound:1548-0774|FD-Score:3.32|P-value:8.94E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:3.48|P-value:5.28E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.80|P-value:1.72E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.81|P-value:1.65E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:4.48|P-value:1.15E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.48|P-value:1.18E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:-4.31|P-value:1.67E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:3.45|P-value:5.78E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.27|P-value:2.83E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:4.60|P-value:7.08E-6 ID:SGTC_990|Compound:3985-0011|FD-Score:4.21|P-value:3.56E-5 ID:SGTC_992|Compound:3909-8734|FD-Score:4.70|P-value:4.45E-6 ID:SGTC_994|Compound:3910-0338|FD-Score:15.10|P-value:9.36E-47 ID:SGTC_995|Compound:3931-2021|FD-Score:13.40|P-value:1.81E-37 ID:SGTC_998|Compound:3996-0085|FD-Score:5.78|P-value:2.24E-8 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:3.32|P-value:8.78E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.57|P-value:3.82E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.49|P-value:5.04E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.90|P-value:1.18E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:4.11|P-value:5.23E-5 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.89|P-value:9.13E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:-3.22|P-value:9.39E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:6.35|P-value:9.15E-10 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.32|P-value:2.30E-5 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.36|P-value:7.92E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.92|P-value:1.08E-8 ID:SGTC_1712|Compound:st032288|FD-Score:4.44|P-value:1.39E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.56|P-value:8.44E-6 ID:SGTC_1768|Compound:st044301|FD-Score:-4.36|P-value:1.36E-5 ID:SGTC_1843|Compound:st055809|FD-Score:5.30|P-value:2.66E-7 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.47|P-value:5.45E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.92|P-value:1.08E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.86|P-value:1.38E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.55|P-value:4.11E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.66|P-value:2.85E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.49|P-value:3.86E-4 ID:SGTC_2299|Compound:7976368|FD-Score:7.56|P-value:3.85E-13 ID:SGTC_2334|Compound:9017922|FD-Score:3.72|P-value:2.24E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:4.68|P-value:5.05E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:8.26|P-value:2.37E-15 ID:SGTC_2584|Compound:androsterone|FD-Score:7.01|P-value:1.60E-11 ID:SGTC_2619|Compound:obliquin|FD-Score:4.34|P-value:2.15E-5 ID:SGTC_2646|Compound:retinol|FD-Score:3.30|P-value:9.65E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.30|P-value:9.52E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.50|P-value:4.89E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.79|P-value:1.78E-4 ID:SGTC_2941|Compound:9056145|FD-Score:6.72|P-value:1.01E-10 ID:SGTC_2945|Compound:9067681|FD-Score:-4.55|P-value:6.15E-6 ID:SGTC_2948|Compound:9070189|FD-Score:6.60|P-value:2.05E-10 ID:SGTC_2951|Compound:9072950|FD-Score:-3.98|P-value:6.44E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.59|P-value:7.25E-6 ID:SGTC_3025|Compound:9087895|FD-Score:5.90|P-value:1.18E-8 ID:SGTC_3027|Compound:9089541|FD-Score:-3.38|P-value:5.62E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-3.30|P-value:7.38E-4 ID:SGTC_3122|Compound:9126309|FD-Score:-3.65|P-value:2.23E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.54|P-value:4.25E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.62|P-value:3.30E-4 ID:SGTC_3266|Compound:9136860|FD-Score:4.42|P-value:1.50E-5 ID:SGTC_3303|Compound:9125618|FD-Score:6.57|P-value:2.52E-10 ID:SGTC_3310|Compound:9130229|FD-Score:3.52|P-value:4.59E-4 ID:SGTC_3311|Compound:9131146|FD-Score:13.70|P-value:4.52E-39 ID:SGTC_3321|Compound:9137793|FD-Score:7.19|P-value:4.95E-12 ID:SGTC_3341|Compound:9147222|FD-Score:3.48|P-value:5.28E-4 ID:SGTC_3350|Compound:9150499|FD-Score:4.23|P-value:3.30E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:9.76|P-value:1.06E-20 ID:SGTC_138|Compound:0850-0259|FD-Score:6.28|P-value:1.39E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:7.00|P-value:1.69E-11 ID:SGTC_146|Compound:3448-8650|FD-Score:5.32|P-value:2.44E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-5.12|P-value:4.44E-7 ID:SGTC_155|Compound:k072-0202|FD-Score:3.54|P-value:4.33E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.27|P-value:7.93E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:3.35|P-value:8.19E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.94|P-value:1.02E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:7.46|P-value:7.68E-13 ID:SGTC_532|Compound:1548-0774|FD-Score:3.32|P-value:8.94E-4 ID:SGTC_630|Compound:4356-0227|FD-Score:3.48|P-value:5.28E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.80|P-value:1.72E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.81|P-value:1.65E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:4.48|P-value:1.15E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:4.48|P-value:1.18E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:-4.31|P-value:1.67E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:3.45|P-value:5.78E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.27|P-value:2.83E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:4.60|P-value:7.08E-6 ID:SGTC_990|Compound:3985-0011|FD-Score:4.21|P-value:3.56E-5 ID:SGTC_992|Compound:3909-8734|FD-Score:4.70|P-value:4.45E-6 ID:SGTC_994|Compound:3910-0338|FD-Score:15.10|P-value:9.36E-47 ID:SGTC_995|Compound:3931-2021|FD-Score:13.40|P-value:1.81E-37 ID:SGTC_998|Compound:3996-0085|FD-Score:5.78|P-value:2.24E-8 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:3.32|P-value:8.78E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.57|P-value:3.82E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.49|P-value:5.04E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.90|P-value:1.18E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:4.11|P-value:5.23E-5 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.89|P-value:9.13E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:-3.22|P-value:9.39E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:6.35|P-value:9.15E-10 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.32|P-value:2.30E-5 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.36|P-value:7.92E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.92|P-value:1.08E-8 ID:SGTC_1712|Compound:st032288|FD-Score:4.44|P-value:1.39E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.56|P-value:8.44E-6 ID:SGTC_1768|Compound:st044301|FD-Score:-4.36|P-value:1.36E-5 ID:SGTC_1843|Compound:st055809|FD-Score:5.30|P-value:2.66E-7 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.47|P-value:5.45E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.92|P-value:1.08E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.86|P-value:1.38E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.55|P-value:4.11E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.66|P-value:2.85E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.49|P-value:3.86E-4 ID:SGTC_2299|Compound:7976368|FD-Score:7.56|P-value:3.85E-13 ID:SGTC_2334|Compound:9017922|FD-Score:3.72|P-value:2.24E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:4.68|P-value:5.05E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:8.26|P-value:2.37E-15 ID:SGTC_2584|Compound:androsterone|FD-Score:7.01|P-value:1.60E-11 ID:SGTC_2619|Compound:obliquin|FD-Score:4.34|P-value:2.15E-5 ID:SGTC_2646|Compound:retinol|FD-Score:3.30|P-value:9.65E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.30|P-value:9.52E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.50|P-value:4.89E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.79|P-value:1.78E-4 ID:SGTC_2941|Compound:9056145|FD-Score:6.72|P-value:1.01E-10 ID:SGTC_2945|Compound:9067681|FD-Score:-4.55|P-value:6.15E-6 ID:SGTC_2948|Compound:9070189|FD-Score:6.60|P-value:2.05E-10 ID:SGTC_2951|Compound:9072950|FD-Score:-3.98|P-value:6.44E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.59|P-value:7.25E-6 ID:SGTC_3025|Compound:9087895|FD-Score:5.90|P-value:1.18E-8 ID:SGTC_3027|Compound:9089541|FD-Score:-3.38|P-value:5.62E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-3.30|P-value:7.38E-4 ID:SGTC_3122|Compound:9126309|FD-Score:-3.65|P-value:2.23E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.54|P-value:4.25E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.62|P-value:3.30E-4 ID:SGTC_3266|Compound:9136860|FD-Score:4.42|P-value:1.50E-5 ID:SGTC_3303|Compound:9125618|FD-Score:6.57|P-value:2.52E-10 ID:SGTC_3310|Compound:9130229|FD-Score:3.52|P-value:4.59E-4 ID:SGTC_3311|Compound:9131146|FD-Score:13.70|P-value:4.52E-39 ID:SGTC_3321|Compound:9137793|FD-Score:7.19|P-value:4.95E-12 ID:SGTC_3341|Compound:9147222|FD-Score:3.48|P-value:5.28E-4 ID:SGTC_3350|Compound:9150499|FD-Score:4.23|P-value:3.30E-5

Top fitness defect scores for YDR253C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_994 3910-0338 15.10 138.0 µM 9.36E-47 calcium & mitochondrial duress 3 1.42E-51
2 SGTC_3311 9131146 13.70 2.9 µM 4.52E-39 cell wall 8 4.33E-43
3 SGTC_995 3931-2021 13.40 34.6 µM 1.81E-37 RPP1 & pyrimidine depletion 1 2.55E-41
4 SGTC_58 0957-0393 9.76 9.0 µM 1.06E-20 cell wall 83 8.59E-23
5 SGTC_2568 diosmetin 8.26 100.0 µM 2.37E-15 1 7.05E-17
6 SGTC_2299 7976368 7.56 114.9 µM 3.85E-13 4 1.96E-14
7 SGTC_528 3492-0019 7.46 172.0 µM 7.68E-13 calcium & mitochondrial duress 88 4.21E-14
8 SGTC_3321 9137793 7.19 26.8 µM 4.95E-12 cell wall 19 3.30E-13
9 SGTC_2584 androsterone 7.01 48.7 µM 1.60E-11 cell wall 39 1.21E-12
10 SGTC_143 4032-1194 7.00 15.7 µM 1.69E-11 13 1.28E-12
11 SGTC_2941 9056145 6.72 11.2 µM 1.01E-10 4 9.25E-12
12 SGTC_2948 9070189 6.60 9.4 µM 2.05E-10 plasma membrane duress 20 2.03E-11
13 SGTC_3303 9125618 6.57 40.6 µM 2.52E-10 9 2.55E-11
14 SGTC_1341 1486-1360 6.35 280.0 µM 9.15E-10 2 1.06E-10
15 SGTC_138 0850-0259 6.28 40.9 µM 1.39E-9 cell wall 100 1.69E-10
16 SGTC_1597 7-benzyloxygramine 5.92 47.5 µM 1.08E-8 calcium & mitochondrial duress 50 1.63E-9
17 SGTC_3025 9087895 5.90 49.5 µM 1.18E-8 PDR1 14 1.80E-9
18 SGTC_998 3996-0085 5.78 177.0 µM 2.24E-8 4 3.66E-9
19 SGTC_146 3448-8650 5.32 53.1 µM 2.44E-7 NEO1-PIK1 65 5.12E-8
20 SGTC_1843 st055809 5.30 45.5 µM 2.66E-7 3 5.66E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.354 1.65E-99 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.315 2.85E-78 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.312 1.19E-76 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.311 2.55E-76 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.304 1.03E-72 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.298 1.45E-69 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.297 2.68E-69 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.297 4.39E-69 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.290 7.98E-66 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.282 2.65E-62 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.280 1.32E-61 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.279 4.55E-61 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.278 1.13E-60 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.266 1.56E-55 YDL021W GPM2 hom Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.260 5.21E-53 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate