YDR255C / RMD5

Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR255C / RMD5

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.52|P-value:9.08E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.64|P-value:4.46E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.05|P-value:6.09E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:3.67|P-value:4.72E-5 ID:SGTC_105|Compound:0111-0055|FD-Score:-4.44|P-value:9.03E-7 ID:SGTC_117|Compound:lucigenin|FD-Score:-3.62|P-value:4.73E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:-4.45|P-value:8.24E-7 ID:SGTC_188|Compound:3229-0966|FD-Score:3.10|P-value:5.07E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.75|P-value:3.41E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.06|P-value:4.77E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-2.98|P-value:6.44E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:-2.87|P-value:9.79E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.46|P-value:1.19E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.39|P-value:1.61E-4 ID:SGTC_398|Compound:bronopol|FD-Score:-3.00|P-value:6.00E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.11|P-value:6.17E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.90|P-value:1.64E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.08|P-value:5.47E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.33|P-value:2.00E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.03|P-value:5.31E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.01|P-value:6.96E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.26|P-value:2.21E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.30|P-value:2.29E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.89|P-value:1.72E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.31|P-value:2.20E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.08|P-value:7.26E-6 ID:SGTC_1023|Compound:4482-3888|FD-Score:2.93|P-value:9.47E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:7.09|P-value:2.05E-14 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.60|P-value:4.83E-7 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.05|P-value:5.95E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.21|P-value:3.27E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.51|P-value:9.51E-5 ID:SGTC_1221|Compound:2958-7315|FD-Score:-3.53|P-value:7.18E-5 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.02|P-value:6.66E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.24|P-value:2.89E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.42|P-value:1.41E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.02|P-value:6.75E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.09|P-value:5.16E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.11|P-value:4.72E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.42|P-value:1.37E-4 ID:SGTC_1658|Compound:st012944|FD-Score:3.05|P-value:6.05E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.30|P-value:1.90E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.27|P-value:2.54E-4 ID:SGTC_1939|Compound:st074455|FD-Score:5.41|P-value:4.23E-9 ID:SGTC_1941|Compound:st076221|FD-Score:5.21|P-value:1.44E-8 ID:SGTC_2149|Compound:5554786|FD-Score:3.10|P-value:4.97E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.00|P-value:1.06E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.60|P-value:6.41E-5 ID:SGTC_2333|Compound:9017922|FD-Score:3.78|P-value:2.90E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-2.90|P-value:8.86E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.11|P-value:4.82E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:3.39|P-value:1.57E-4 ID:SGTC_2902|Compound:5787006|FD-Score:3.70|P-value:4.22E-5 ID:SGTC_2903|Compound:5923464|FD-Score:-3.77|P-value:2.48E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.46|P-value:1.16E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.33|P-value:2.03E-4 ID:SGTC_3050|Compound:9093513|FD-Score:-3.01|P-value:5.86E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.50|P-value:1.01E-4 ID:SGTC_3309|Compound:9129340|FD-Score:4.71|P-value:2.69E-7 ID:SGTC_43|Compound:0570-0105|FD-Score:3.52|P-value:9.08E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.64|P-value:4.46E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.05|P-value:6.09E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:3.67|P-value:4.72E-5 ID:SGTC_105|Compound:0111-0055|FD-Score:-4.44|P-value:9.03E-7 ID:SGTC_117|Compound:lucigenin|FD-Score:-3.62|P-value:4.73E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:-4.45|P-value:8.24E-7 ID:SGTC_188|Compound:3229-0966|FD-Score:3.10|P-value:5.07E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.75|P-value:3.41E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.06|P-value:4.77E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-2.98|P-value:6.44E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:-2.87|P-value:9.79E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.46|P-value:1.19E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.39|P-value:1.61E-4 ID:SGTC_398|Compound:bronopol|FD-Score:-3.00|P-value:6.00E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.11|P-value:6.17E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.90|P-value:1.64E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.08|P-value:5.47E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.33|P-value:2.00E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.03|P-value:5.31E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.01|P-value:6.96E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.26|P-value:2.21E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.30|P-value:2.29E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.89|P-value:1.72E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.31|P-value:2.20E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.08|P-value:7.26E-6 ID:SGTC_1023|Compound:4482-3888|FD-Score:2.93|P-value:9.47E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:7.09|P-value:2.05E-14 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:4.60|P-value:4.83E-7 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.05|P-value:5.95E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.21|P-value:3.27E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.51|P-value:9.51E-5 ID:SGTC_1221|Compound:2958-7315|FD-Score:-3.53|P-value:7.18E-5 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.02|P-value:6.66E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.24|P-value:2.89E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.42|P-value:1.41E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.02|P-value:6.75E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.09|P-value:5.16E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.11|P-value:4.72E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.42|P-value:1.37E-4 ID:SGTC_1658|Compound:st012944|FD-Score:3.05|P-value:6.05E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.30|P-value:1.90E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.27|P-value:2.54E-4 ID:SGTC_1939|Compound:st074455|FD-Score:5.41|P-value:4.23E-9 ID:SGTC_1941|Compound:st076221|FD-Score:5.21|P-value:1.44E-8 ID:SGTC_2149|Compound:5554786|FD-Score:3.10|P-value:4.97E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.00|P-value:1.06E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.60|P-value:6.41E-5 ID:SGTC_2333|Compound:9017922|FD-Score:3.78|P-value:2.90E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-2.90|P-value:8.86E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.11|P-value:4.82E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:3.39|P-value:1.57E-4 ID:SGTC_2902|Compound:5787006|FD-Score:3.70|P-value:4.22E-5 ID:SGTC_2903|Compound:5923464|FD-Score:-3.77|P-value:2.48E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.46|P-value:1.16E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.33|P-value:2.03E-4 ID:SGTC_3050|Compound:9093513|FD-Score:-3.01|P-value:5.86E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.50|P-value:1.01E-4 ID:SGTC_3309|Compound:9129340|FD-Score:4.71|P-value:2.69E-7

Top fitness defect scores for YDR255C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1026 k007-1105 7.09 30.6 µM 2.05E-14 ERAD & cell cycle 25 6.90E-13
2 SGTC_1939 st074455 5.41 53.6 µM 4.23E-9 7 3.23E-8
3 SGTC_1941 st076221 5.21 61.8 µM 1.44E-8 sphingolipid biosynthesis & PDR1 41 9.49E-8
4 SGTC_3309 9129340 4.71 25.8 µM 2.69E-7 7 1.25E-6
5 SGTC_1110 3-hydroxy-3'-methoxyflavone 4.60 1.2 µM 4.83E-7 iron homeostasis 30 2.10E-6
6 SGTC_426 0988-0037 4.11 14.6 µM 6.17E-6 ERAD & cell cycle 29 1.99E-5
7 SGTC_946 3434-1334 4.08 87.4 µM 7.26E-6 28 2.29E-5
8 SGTC_2296 7971656 4.00 126.5 µM 1.06E-5 41 3.21E-5
9 SGTC_453 enantio-paf c16 3.90 10.0 µM 1.64E-5 146 4.72E-5
10 SGTC_669 0108-0021 3.89 26.2 µM 1.72E-5 fatty acid desaturase (OLE1) 84 4.92E-5
11 SGTC_2333 9017922 3.78 200.0 µM 2.90E-5 32 7.79E-5
12 SGTC_207 4185-0010 3.75 4.5 µM 3.41E-5 endomembrane recycling 97 8.99E-5
13 SGTC_2902 5787006 3.70 60.5 µM 4.22E-5 Golgi 34 1.09E-4
14 SGTC_59 0330-0160 3.67 1.3 µM 4.72E-5 131 1.20E-4
15 SGTC_2322 7413319 3.60 38.2 µM 6.41E-5 ERAD & cell cycle 180 1.57E-4
16 SGTC_43 0570-0105 3.52 72.5 µM 9.08E-5 ERG2 129 2.14E-4
17 SGTC_1211 1056-0033 3.51 32.3 µM 9.51E-5 16 2.23E-4
18 SGTC_3306 9127676 3.50 59.8 µM 1.01E-4 36 2.36E-4
19 SGTC_2932 9016711 3.46 44.5 µM 1.16E-4 63 2.65E-4
20 SGTC_263 alizarin 3.46 107.9 µM 1.19E-4 iron homeostasis 82 2.71E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.192 3.84E-29 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.178 3.15E-25 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.177 4.52E-25 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.141 2.36E-16 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.134 6.42E-15 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.131 2.31E-14 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.127 1.49E-13 YMR135C GID8 hom Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
0.127 1.60E-13 YDR073W SNF11 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
0.118 5.76E-12 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.112 7.77E-11 YNL259C ATX1 hom Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake
0.108 4.18E-10 YNL026W SAM50 het Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
0.107 4.50E-10 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.107 4.84E-10 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.103 2.11E-9 YDL133C-A RPL41B hom Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
0.101 4.04E-9 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress