YDR266C / HEL2

RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR266C / HEL2

Click on Significant Values for Screen Details ID:SGTC_439|Compound:amiodarone|FD-Score:-3.30|P-value:5.11E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.80|P-value:8.62E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.54|P-value:2.40E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.38|P-value:4.21E-4 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:4.67|P-value:2.02E-6 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.13|P-value:9.12E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.86|P-value:7.04E-5 ID:SGTC_1689|Compound:st020265|FD-Score:6.28|P-value:2.72E-10 ID:SGTC_1721|Compound:st082152|FD-Score:-3.51|P-value:2.35E-4 ID:SGTC_1993|Compound:st073995|FD-Score:5.05|P-value:2.97E-7 ID:SGTC_2018|Compound:5133068|FD-Score:4.10|P-value:2.62E-5 ID:SGTC_2023|Compound:5144124|FD-Score:4.41|P-value:6.76E-6 ID:SGTC_2024|Compound:5144227|FD-Score:-4.75|P-value:1.13E-6 ID:SGTC_2081|Compound:5214970|FD-Score:4.72|P-value:1.52E-6 ID:SGTC_2100|Compound:5377183|FD-Score:3.36|P-value:4.62E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:-3.73|P-value:1.00E-4 ID:SGTC_2526|Compound:patulin|FD-Score:3.50|P-value:2.79E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.64|P-value:1.46E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.16|P-value:9.02E-4 ID:SGTC_3001|Compound:9076041|FD-Score:-3.33|P-value:4.48E-4 ID:SGTC_3019|Compound:9081971|FD-Score:4.12|P-value:2.35E-5 ID:SGTC_3227|Compound:9130768|FD-Score:3.61|P-value:1.84E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.30|P-value:5.11E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.80|P-value:8.62E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.54|P-value:2.40E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.38|P-value:4.21E-4 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:4.67|P-value:2.02E-6 ID:SGTC_1445|Compound:4239-0156|FD-Score:-3.13|P-value:9.12E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.86|P-value:7.04E-5 ID:SGTC_1689|Compound:st020265|FD-Score:6.28|P-value:2.72E-10 ID:SGTC_1721|Compound:st082152|FD-Score:-3.51|P-value:2.35E-4 ID:SGTC_1993|Compound:st073995|FD-Score:5.05|P-value:2.97E-7 ID:SGTC_2018|Compound:5133068|FD-Score:4.10|P-value:2.62E-5 ID:SGTC_2023|Compound:5144124|FD-Score:4.41|P-value:6.76E-6 ID:SGTC_2024|Compound:5144227|FD-Score:-4.75|P-value:1.13E-6 ID:SGTC_2081|Compound:5214970|FD-Score:4.72|P-value:1.52E-6 ID:SGTC_2100|Compound:5377183|FD-Score:3.36|P-value:4.62E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:-3.73|P-value:1.00E-4 ID:SGTC_2526|Compound:patulin|FD-Score:3.50|P-value:2.79E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.64|P-value:1.46E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.16|P-value:9.02E-4 ID:SGTC_3001|Compound:9076041|FD-Score:-3.33|P-value:4.48E-4 ID:SGTC_3019|Compound:9081971|FD-Score:4.12|P-value:2.35E-5 ID:SGTC_3227|Compound:9130768|FD-Score:3.61|P-value:1.84E-4

Top fitness defect scores for YDR266C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1689 st020265 6.28 53.5 µM 2.72E-10 2 1.75E-10
2 SGTC_1993 st073995 5.05 70.8 µM 2.97E-7 6 2.18E-7
3 SGTC_2081 5214970 4.72 32.3 µM 1.52E-6 11 1.16E-6
4 SGTC_1065 prochlorperazine maleate 4.67 2.5 µM 2.02E-6 5 1.54E-6
5 SGTC_2023 5144124 4.41 124.0 µM 6.76E-6 18 5.29E-6
6 SGTC_3019 9081971 4.12 49.5 µM 2.35E-5 14 1.89E-5
7 SGTC_2018 5133068 4.10 143.0 µM 2.62E-5 18 2.10E-5
8 SGTC_1628 st004252 3.86 77.7 µM 7.04E-5 23 5.77E-5
9 SGTC_536 3852-0578 3.80 10.6 µM 8.62E-5 27 7.09E-5
10 SGTC_3227 9130768 3.61 49.5 µM 1.84E-4 11 1.53E-4
11 SGTC_553 k007-0256 3.54 45.7 µM 2.40E-4 heme biosynthesis & mitochondrial translocase 32 2.01E-4
12 SGTC_2526 patulin 3.50 37.6 µM 2.79E-4 121 2.35E-4
13 SGTC_690 glyoxal 3.38 20.3 µM 4.21E-4 118 3.58E-4
14 SGTC_2100 5377183 3.36 190.0 µM 4.62E-4 60S ribosome export 28 3.93E-4
15 SGTC_2956 9076196 3.16 2.5 µM 9.02E-4 60 7.79E-4
16 SGTC_2058 5236698 3.08 133.0 µM 0.00119 64 0.00104
17 SGTC_554 4483-2211 3.04 187.0 µM 0.00138 51 0.00120
18 SGTC_1986 st071812 3.01 45.0 µM 0.00148 32 0.00129
19 SGTC_1949 st076024 3.01 69.1 µM 0.00150 67 0.00131
20 SGTC_1632 st005650 2.99 90.0 µM 0.00158 71 0.00138

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.03E-17 YNR020C ATP23 hom Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
0.131 3.32E-14 YDR287W INM2 hom Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
0.127 1.39E-13 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.125 3.65E-13 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.123 7.24E-13 YPR073C LTP1 hom Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
0.117 8.56E-12 YDL123W SNA4 hom Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated
0.113 6.00E-11 YPR016C TIF6 het Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
0.112 7.58E-11 YIL166C_p YIL166C_p hom Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
0.107 5.07E-10 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.105 9.27E-10 YPR174C YPR174C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
0.103 2.61E-9 YOR090C PTC5 hom Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p
0.102 3.31E-9 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.097 1.52E-8 YJL110C GZF3 hom GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
0.097 2.11E-8 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.096 2.36E-8 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress