HIPHOP chemogenomics database

YDR266C / HEL2

RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR266C / HEL2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR266C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1689	st020265	6.28	53.5 µM	2.72E-10		2	1.75E-10
2	SGTC_1993	st073995	5.05	70.8 µM	2.97E-7		6	2.18E-7
3	SGTC_2081	5214970	4.72	32.3 µM	1.52E-6		11	1.16E-6
4	SGTC_1065	prochlorperazine maleate	4.67	2.5 µM	2.02E-6		5	1.54E-6
5	SGTC_2023	5144124	4.41	124.0 µM	6.76E-6		18	5.29E-6
6	SGTC_3019	9081971	4.12	49.5 µM	2.35E-5		14	1.89E-5
7	SGTC_2018	5133068	4.10	143.0 µM	2.62E-5		18	2.10E-5
8	SGTC_1628	st004252	3.86	77.7 µM	7.04E-5		23	5.77E-5
9	SGTC_536	3852-0578	3.80	10.6 µM	8.62E-5		27	7.09E-5
10	SGTC_3227	9130768	3.61	49.5 µM	1.84E-4		11	1.53E-4
11	SGTC_553	k007-0256	3.54	45.7 µM	2.40E-4	heme biosynthesis & mitochondrial translocase	32	2.01E-4
12	SGTC_2526	patulin	3.50	37.6 µM	2.79E-4		121	2.35E-4
13	SGTC_690	glyoxal	3.38	20.3 µM	4.21E-4		118	3.58E-4
14	SGTC_2100	5377183	3.36	190.0 µM	4.62E-4	60S ribosome export	28	3.93E-4
15	SGTC_2956	9076196	3.16	2.5 µM	9.02E-4		60	7.79E-4
16	SGTC_2058	5236698	3.08	133.0 µM	0.00119		64	0.00104
17	SGTC_554	4483-2211	3.04	187.0 µM	0.00138		51	0.00120
18	SGTC_1986	st071812	3.01	45.0 µM	0.00148		32	0.00129
19	SGTC_1949	st076024	3.01	69.1 µM	0.00150		67	0.00131
20	SGTC_1632	st005650	2.99	90.0 µM	0.00158		71	0.00138

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.147	1.03E-17	YNR020C	ATP23	hom	Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
0.131	3.32E-14	YDR287W	INM2	hom	Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
0.127	1.39E-13	YMR080C	NAM7	hom	ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.125	3.65E-13	YMR067C	UBX4	hom	UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.123	7.24E-13	YPR073C	LTP1	hom	Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
0.117	8.56E-12	YDL123W	SNA4	hom	Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated
0.113	6.00E-11	YPR016C	TIF6	het	Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
0.112	7.58E-11	YIL166C_p	YIL166C_p	hom	Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
0.107	5.07E-10	YMR132C_p	JLP2_p	hom	Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.105	9.27E-10	YPR174C	YPR174C	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
0.103	2.61E-9	YOR090C	PTC5	hom	Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p
0.102	3.31E-9	YGR190C_d	YGR190C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.097	1.52E-8	YJL110C	GZF3	hom	GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
0.097	2.11E-8	YLL054C_p	YLL054C_p	hom	Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.096	2.36E-8	YOR298C-A	MBF1	hom	Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress