YDR277C / MTH1

Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR277C / MTH1

Click on Significant Values for Screen Details ID:SGTC_426|Compound:0988-0037|FD-Score:3.21|P-value:3.72E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-4.23|P-value:3.76E-6 ID:SGTC_433|Compound:pimozide|FD-Score:3.48|P-value:1.27E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:5.65|P-value:1.39E-9 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.77|P-value:3.78E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:-4.93|P-value:9.30E-8 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.91|P-value:1.99E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.16|P-value:4.49E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.50|P-value:1.05E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.11|P-value:4.93E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.04|P-value:7.09E-4 ID:SGTC_1563|Compound:conessine|FD-Score:5.26|P-value:1.57E-8 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.82|P-value:2.06E-7 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.30|P-value:3.11E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:2.98|P-value:8.93E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.36|P-value:1.85E-4 ID:SGTC_1852|Compound:st056254|FD-Score:4.43|P-value:1.65E-6 ID:SGTC_1958|Compound:st076513|FD-Score:-4.81|P-value:1.80E-7 ID:SGTC_1986|Compound:st071812|FD-Score:4.42|P-value:1.66E-6 ID:SGTC_2002|Compound:4023503|FD-Score:6.29|P-value:1.78E-11 ID:SGTC_2003|Compound:4023581|FD-Score:-3.67|P-value:5.08E-5 ID:SGTC_2008|Compound:4012253|FD-Score:-6.23|P-value:2.26E-11 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.93|P-value:1.59E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.66|P-value:5.18E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-2.92|P-value:9.65E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.02|P-value:6.79E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.32|P-value:2.48E-4 ID:SGTC_2537|Compound:larixol|FD-Score:5.64|P-value:1.49E-9 ID:SGTC_2568|Compound:diosmetin|FD-Score:8.13|P-value:5.87E-18 ID:SGTC_2569|Compound:tetrandrine|FD-Score:5.75|P-value:7.33E-10 ID:SGTC_3001|Compound:9076041|FD-Score:-3.31|P-value:2.26E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.13|P-value:4.47E-4 ID:SGTC_3133|Compound:9127736|FD-Score:-3.06|P-value:5.83E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:3.21|P-value:3.72E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-4.23|P-value:3.76E-6 ID:SGTC_433|Compound:pimozide|FD-Score:3.48|P-value:1.27E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:5.65|P-value:1.39E-9 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.77|P-value:3.78E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:-4.93|P-value:9.30E-8 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.91|P-value:1.99E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.16|P-value:4.49E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.50|P-value:1.05E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.11|P-value:4.93E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.04|P-value:7.09E-4 ID:SGTC_1563|Compound:conessine|FD-Score:5.26|P-value:1.57E-8 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.82|P-value:2.06E-7 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.30|P-value:3.11E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:2.98|P-value:8.93E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.36|P-value:1.85E-4 ID:SGTC_1852|Compound:st056254|FD-Score:4.43|P-value:1.65E-6 ID:SGTC_1958|Compound:st076513|FD-Score:-4.81|P-value:1.80E-7 ID:SGTC_1986|Compound:st071812|FD-Score:4.42|P-value:1.66E-6 ID:SGTC_2002|Compound:4023503|FD-Score:6.29|P-value:1.78E-11 ID:SGTC_2003|Compound:4023581|FD-Score:-3.67|P-value:5.08E-5 ID:SGTC_2008|Compound:4012253|FD-Score:-6.23|P-value:2.26E-11 ID:SGTC_2129|Compound:girgensonine|FD-Score:-3.93|P-value:1.59E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.66|P-value:5.18E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-2.92|P-value:9.65E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.02|P-value:6.79E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.32|P-value:2.48E-4 ID:SGTC_2537|Compound:larixol|FD-Score:5.64|P-value:1.49E-9 ID:SGTC_2568|Compound:diosmetin|FD-Score:8.13|P-value:5.87E-18 ID:SGTC_2569|Compound:tetrandrine|FD-Score:5.75|P-value:7.33E-10 ID:SGTC_3001|Compound:9076041|FD-Score:-3.31|P-value:2.26E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.13|P-value:4.47E-4 ID:SGTC_3133|Compound:9127736|FD-Score:-3.06|P-value:5.83E-4

Top fitness defect scores for YDR277C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2568 diosmetin 8.13 100.0 µM 5.87E-18 2 2.20E-16
2 SGTC_2002 4023503 6.29 131.0 µM 1.78E-11 mitochondrial processes 6 1.55E-10
3 SGTC_2569 tetrandrine 5.75 100.0 µM 7.33E-10 3 4.47E-9
4 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 5.65 8.0 µM 1.39E-9 8 7.98E-9
5 SGTC_2537 larixol 5.64 68.9 µM 1.49E-9 6 8.48E-9
6 SGTC_1563 conessine 5.26 56.1 µM 1.57E-8 3 7.14E-8
7 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 4.82 85.4 µM 2.06E-7 10 7.30E-7
8 SGTC_1852 st056254 4.43 31.4 µM 1.65E-6 6 4.80E-6
9 SGTC_1986 st071812 4.42 45.0 µM 1.66E-6 2 4.84E-6
10 SGTC_1569 carminic acid 4.30 40.6 µM 3.11E-6 12 8.53E-6
11 SGTC_457 grayanotoxin iii 3.91 135.0 µM 1.99E-5 69 4.59E-5
12 SGTC_436 n,n-dimethylsphingosine 3.77 3.3 µM 3.78E-5 33 8.21E-5
13 SGTC_433 pimozide 3.48 108.0 µM 1.27E-4 30 2.48E-4
14 SGTC_2534 helenine 3.32 20.1 µM 2.48E-4 RSC complex & mRNA processing 25 4.54E-4
15 SGTC_426 0988-0037 3.21 14.6 µM 3.72E-4 ERAD & cell cycle 66 6.55E-4
16 SGTC_932 2857-0803 3.16 154.0 µM 4.49E-4 60 7.78E-4
17 SGTC_1212 0520-0165 3.04 585.0 µM 7.09E-4 84 0.00118
18 SGTC_1572 epiandrosterone 2.98 68.9 µM 8.93E-4 63 0.00145
19 SGTC_533 1222-0222 2.90 6.7 µM 0.00117 PDR1 76 0.00187
20 SGTC_983 1491-0821 2.88 12.2 µM 0.00125 50 0.00197

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.207 1.14E-33 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.185 3.89E-27 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.170 4.00E-23 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.163 2.29E-21 YDR387C_p YDR387C_p hom Putative transporter, member of the sugar porter family; YDR387C is not an essential gene
0.160 9.35E-21 YGL057C GEP7 hom Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.160 1.52E-20 YGL263W COS12 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.153 5.88E-19 YGR201C_p YGR201C_p hom Putative protein of unknown function
0.150 2.00E-18 YGL245W GUS1 het Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
0.146 1.96E-17 YKL030W_d YKL030W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
0.143 7.72E-17 YDL086W_p YDL086W_p hom Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
0.134 6.11E-15 YGR042W YGR042W hom Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
0.131 3.41E-14 YGR018C_p YGR018C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
0.126 2.67E-13 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.125 4.52E-13 YMR115W MGR3 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.120 3.13E-12 YBL002W HTB2 hom Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation