YDR279W / RNH202

Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR279W / RNH202

Click on Significant Values for Screen Details ID:SGTC_589|Compound:1227-0070|FD-Score:3.11|P-value:3.99E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.98|P-value:9.54E-6 ID:SGTC_781|Compound:4335-2806|FD-Score:2.89|P-value:9.31E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.01|P-value:8.03E-6 ID:SGTC_1124|Compound:fisetin|FD-Score:5.75|P-value:3.33E-10 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.01|P-value:5.84E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.51|P-value:1.60E-9 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.23|P-value:2.55E-4 ID:SGTC_1994|Compound:st072947|FD-Score:-3.12|P-value:4.64E-4 ID:SGTC_2124|Compound:5246320|FD-Score:2.95|P-value:7.31E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-7.44|P-value:1.06E-15 ID:SGTC_2337|Compound:9009472|FD-Score:2.92|P-value:8.40E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.21|P-value:2.75E-4 ID:SGTC_2443|Compound:5493264|FD-Score:-4.14|P-value:5.18E-6 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.17|P-value:3.72E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.70|P-value:3.37E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.17|P-value:3.17E-4 ID:SGTC_2820|Compound:7989630|FD-Score:-3.19|P-value:3.47E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-5.44|P-value:3.38E-9 ID:SGTC_2918|Compound:7977171|FD-Score:-3.30|P-value:2.24E-4 ID:SGTC_2922|Compound:7999257|FD-Score:4.55|P-value:5.06E-7 ID:SGTC_2925|Compound:9010536|FD-Score:-2.97|P-value:7.88E-4 ID:SGTC_2929|Compound:9013348|FD-Score:4.86|P-value:8.91E-8 ID:SGTC_2930|Compound:9013230|FD-Score:3.87|P-value:1.55E-5 ID:SGTC_2943|Compound:9068529|FD-Score:3.36|P-value:1.48E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.27|P-value:2.19E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.23|P-value:2.99E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-2.93|P-value:9.32E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.13|P-value:4.40E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.14|P-value:4.25E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.32|P-value:1.76E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.87|P-value:1.56E-5 ID:SGTC_3348|Compound:9149464|FD-Score:-5.05|P-value:3.74E-8 ID:SGTC_3351|Compound:9150312|FD-Score:-3.13|P-value:4.43E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-2.96|P-value:8.38E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.11|P-value:3.99E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.98|P-value:9.54E-6 ID:SGTC_781|Compound:4335-2806|FD-Score:2.89|P-value:9.31E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.01|P-value:8.03E-6 ID:SGTC_1124|Compound:fisetin|FD-Score:5.75|P-value:3.33E-10 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.01|P-value:5.84E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.51|P-value:1.60E-9 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.23|P-value:2.55E-4 ID:SGTC_1994|Compound:st072947|FD-Score:-3.12|P-value:4.64E-4 ID:SGTC_2124|Compound:5246320|FD-Score:2.95|P-value:7.31E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-7.44|P-value:1.06E-15 ID:SGTC_2337|Compound:9009472|FD-Score:2.92|P-value:8.40E-4 ID:SGTC_2371|Compound:9071574|FD-Score:3.21|P-value:2.75E-4 ID:SGTC_2443|Compound:5493264|FD-Score:-4.14|P-value:5.18E-6 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.17|P-value:3.72E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.70|P-value:3.37E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.17|P-value:3.17E-4 ID:SGTC_2820|Compound:7989630|FD-Score:-3.19|P-value:3.47E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-5.44|P-value:3.38E-9 ID:SGTC_2918|Compound:7977171|FD-Score:-3.30|P-value:2.24E-4 ID:SGTC_2922|Compound:7999257|FD-Score:4.55|P-value:5.06E-7 ID:SGTC_2925|Compound:9010536|FD-Score:-2.97|P-value:7.88E-4 ID:SGTC_2929|Compound:9013348|FD-Score:4.86|P-value:8.91E-8 ID:SGTC_2930|Compound:9013230|FD-Score:3.87|P-value:1.55E-5 ID:SGTC_2943|Compound:9068529|FD-Score:3.36|P-value:1.48E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.27|P-value:2.19E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.23|P-value:2.99E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-2.93|P-value:9.32E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.13|P-value:4.40E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.14|P-value:4.25E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.32|P-value:1.76E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.87|P-value:1.56E-5 ID:SGTC_3348|Compound:9149464|FD-Score:-5.05|P-value:3.74E-8 ID:SGTC_3351|Compound:9150312|FD-Score:-3.13|P-value:4.43E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-2.96|P-value:8.38E-4

Top fitness defect scores for YDR279W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1124 fisetin 5.75 1.2 µM 3.33E-10 8 4.43E-9
2 SGTC_1134 3477-0009 5.51 42.5 µM 1.60E-9 12 1.75E-8
3 SGTC_2929 9013348 4.86 24.6 µM 8.91E-8 6 5.87E-7
4 SGTC_2922 7999257 4.55 32.5 µM 5.06E-7 6 2.68E-6
5 SGTC_1119 parthenolide 4.01 318.5 nM 8.03E-6 TSC3-RPN4 35 3.01E-5
6 SGTC_604 1000-0114 3.98 314.0 µM 9.54E-6 21 3.50E-5
7 SGTC_2930 9013230 3.87 14.9 µM 1.55E-5 heme biosynthesis & mitochondrial translocase 42 5.35E-5
8 SGTC_3341 9147222 3.87 25.9 µM 1.56E-5 RNA processing & uracil transport 18 5.40E-5
9 SGTC_2615 purpurogallin-4-carboxylic acid 3.70 100.0 µM 3.37E-5 redox potentiating 74 1.06E-4
10 SGTC_2943 9068529 3.36 45.3 µM 1.48E-4 44 3.87E-4
11 SGTC_3306 9127676 3.32 59.8 µM 1.76E-4 46 4.49E-4
12 SGTC_2949 9072542 3.27 2.2 µM 2.19E-4 TSC3-RPN4 72 5.44E-4
13 SGTC_1500 4488-1866 3.23 171.0 µM 2.55E-4 RPP1 & pyrimidine depletion 46 6.22E-4
14 SGTC_2371 9071574 3.21 200.0 µM 2.75E-4 31 6.64E-4
15 SGTC_2621 lithochol-11-enic acid 3.17 100.0 µM 3.17E-4 67 7.52E-4
16 SGTC_589 1227-0070 3.11 34.1 µM 3.99E-4 57 9.21E-4
17 SGTC_1128 3448-9350 3.01 235.0 µM 5.84E-4 66 0.00129
18 SGTC_2124 5246320 2.95 200.0 µM 7.31E-4 heme biosynthesis & mitochondrial translocase 61 0.00157
19 SGTC_2337 9009472 2.92 200.0 µM 8.40E-4 85 0.00177
20 SGTC_781 4335-2806 2.89 94.4 µM 9.31E-4 63 0.00194

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.60E-13 YIL166C_p YIL166C_p hom Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene
0.114 3.95E-11 YLR054C OSW2 hom Protein of unknown function proposed to be involved in the assembly of the spore wall
0.114 4.21E-11 YBR239C_p ERT1_p hom Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition
0.107 4.71E-10 YHR175W-A_p YHR175W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.105 1.03E-9 YGR177C ATF2 hom Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
0.103 1.85E-9 YPR174C YPR174C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
0.103 2.37E-9 YGR251W NOP19 het Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
0.102 3.10E-9 YOR382W FIT2 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.101 4.21E-9 YGR197C SNG1 hom Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
0.100 5.87E-9 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.097 1.69E-8 YOR008C SLG1 hom Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.096 2.34E-8 YPL099C AIM43 hom Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
0.094 5.71E-8 YBR157C ICS2 hom Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization
0.093 5.83E-8 YKR046C PET10 hom Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
0.093 7.88E-8 YGR038W ORM1 hom Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control