YDR280W / RRP45

Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR280W / RRP45

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:4.99|P-value:3.18E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:-4.51|P-value:4.29E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:4.01|P-value:7.39E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-6.15|P-value:6.03E-8 ID:SGTC_53|Compound:0973-0023|FD-Score:4.44|P-value:2.00E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:5.53|P-value:4.34E-6 ID:SGTC_84|Compound:0443-0269|FD-Score:4.42|P-value:2.10E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.29|P-value:3.22E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.65|P-value:6.61E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:6.77|P-value:2.05E-8 ID:SGTC_130|Compound:0136-0219|FD-Score:5.54|P-value:4.04E-6 ID:SGTC_154|Compound:3389-1638|FD-Score:4.42|P-value:2.10E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.82|P-value:5.68E-5 ID:SGTC_230|Compound:MPP+|FD-Score:4.58|P-value:1.28E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.51|P-value:1.57E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:16.80|P-value:8.56E-44 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:12.00|P-value:3.88E-23 ID:SGTC_274|Compound:sirtinol|FD-Score:5.39|P-value:7.32E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:5.52|P-value:4.46E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:5.28|P-value:1.10E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:8.80|P-value:3.49E-13 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:6.18|P-value:2.99E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:12.90|P-value:1.16E-26 ID:SGTC_617|Compound:3770-0098|FD-Score:6.21|P-value:2.66E-7 ID:SGTC_625|Compound:k213-0058|FD-Score:5.13|P-value:1.94E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.35|P-value:1.89E-6 ID:SGTC_790|Compound:1575-0012|FD-Score:-3.86|P-value:3.62E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:4.42|P-value:2.10E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:7.02|P-value:6.16E-9 ID:SGTC_923|Compound:1900-2434|FD-Score:7.05|P-value:5.40E-9 ID:SGTC_1074|Compound:carmofur|FD-Score:12.60|P-value:1.90E-25 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:11.80|P-value:2.00E-22 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.27|P-value:3.34E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:9.37|P-value:9.65E-15 ID:SGTC_1293|Compound:1000-1278|FD-Score:4.07|P-value:6.20E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:6.03|P-value:5.56E-7 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.91|P-value:3.11E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:4.12|P-value:5.22E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.35|P-value:2.65E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.85|P-value:3.68E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-4.55|P-value:3.83E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:8.58|P-value:1.30E-12 ID:SGTC_1745|Compound:st081495|FD-Score:5.74|P-value:1.89E-6 ID:SGTC_1755|Compound:st041960|FD-Score:4.11|P-value:5.37E-4 ID:SGTC_1814|Compound:st052110|FD-Score:4.33|P-value:2.79E-4 ID:SGTC_1817|Compound:st052116|FD-Score:4.43|P-value:2.08E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.97|P-value:8.18E-4 ID:SGTC_1932|Compound:st074806|FD-Score:5.88|P-value:1.04E-6 ID:SGTC_2173|Compound:5763948|FD-Score:4.36|P-value:2.53E-4 ID:SGTC_2192|Compound:5915882|FD-Score:5.87|P-value:1.10E-6 ID:SGTC_2342|Compound:7990637|FD-Score:4.75|P-value:7.13E-5 ID:SGTC_2393|Compound:9031399|FD-Score:4.47|P-value:1.82E-4 ID:SGTC_2460|Compound:5552655|FD-Score:5.29|P-value:1.05E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:-4.07|P-value:1.88E-4 ID:SGTC_2692|Compound:st077426|FD-Score:4.49|P-value:1.69E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-5.01|P-value:7.06E-6 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.58|P-value:1.27E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.65|P-value:2.66E-5 ID:SGTC_2818|Compound:7996756|FD-Score:4.63|P-value:1.07E-4 ID:SGTC_2916|Compound:7875993|FD-Score:4.07|P-value:6.16E-4 ID:SGTC_2970|Compound:9090938|FD-Score:6.96|P-value:8.32E-9 ID:SGTC_3052|Compound:9092717|FD-Score:4.41|P-value:2.19E-4 ID:SGTC_3056|Compound:9094900|FD-Score:-4.39|P-value:6.59E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-3.56|P-value:8.71E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.92|P-value:3.02E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.74|P-value:7.43E-5 ID:SGTC_3233|Compound:9132340|FD-Score:3.99|P-value:7.81E-4 ID:SGTC_3286|Compound:9117135|FD-Score:4.54|P-value:1.46E-4 ID:SGTC_3306|Compound:9127676|FD-Score:6.59|P-value:4.71E-8 ID:SGTC_3310|Compound:9130229|FD-Score:4.15|P-value:4.90E-4 ID:SGTC_3337|Compound:9144342|FD-Score:6.90|P-value:1.12E-8 ID:SGTC_3341|Compound:9147222|FD-Score:9.28|P-value:1.73E-14 ID:SGTC_39|Compound:0986-0283|FD-Score:4.99|P-value:3.18E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:-4.51|P-value:4.29E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:4.01|P-value:7.39E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-6.15|P-value:6.03E-8 ID:SGTC_53|Compound:0973-0023|FD-Score:4.44|P-value:2.00E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:5.53|P-value:4.34E-6 ID:SGTC_84|Compound:0443-0269|FD-Score:4.42|P-value:2.10E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.29|P-value:3.22E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.65|P-value:6.61E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:6.77|P-value:2.05E-8 ID:SGTC_130|Compound:0136-0219|FD-Score:5.54|P-value:4.04E-6 ID:SGTC_154|Compound:3389-1638|FD-Score:4.42|P-value:2.10E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.82|P-value:5.68E-5 ID:SGTC_230|Compound:MPP+|FD-Score:4.58|P-value:1.28E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.51|P-value:1.57E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:16.80|P-value:8.56E-44 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:12.00|P-value:3.88E-23 ID:SGTC_274|Compound:sirtinol|FD-Score:5.39|P-value:7.32E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:5.52|P-value:4.46E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:5.28|P-value:1.10E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:8.80|P-value:3.49E-13 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:6.18|P-value:2.99E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:12.90|P-value:1.16E-26 ID:SGTC_617|Compound:3770-0098|FD-Score:6.21|P-value:2.66E-7 ID:SGTC_625|Compound:k213-0058|FD-Score:5.13|P-value:1.94E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.35|P-value:1.89E-6 ID:SGTC_790|Compound:1575-0012|FD-Score:-3.86|P-value:3.62E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:4.42|P-value:2.10E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:7.02|P-value:6.16E-9 ID:SGTC_923|Compound:1900-2434|FD-Score:7.05|P-value:5.40E-9 ID:SGTC_1074|Compound:carmofur|FD-Score:12.60|P-value:1.90E-25 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:11.80|P-value:2.00E-22 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.27|P-value:3.34E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:9.37|P-value:9.65E-15 ID:SGTC_1293|Compound:1000-1278|FD-Score:4.07|P-value:6.20E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:6.03|P-value:5.56E-7 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.91|P-value:3.11E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:4.12|P-value:5.22E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.35|P-value:2.65E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.85|P-value:3.68E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-4.55|P-value:3.83E-5 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:8.58|P-value:1.30E-12 ID:SGTC_1745|Compound:st081495|FD-Score:5.74|P-value:1.89E-6 ID:SGTC_1755|Compound:st041960|FD-Score:4.11|P-value:5.37E-4 ID:SGTC_1814|Compound:st052110|FD-Score:4.33|P-value:2.79E-4 ID:SGTC_1817|Compound:st052116|FD-Score:4.43|P-value:2.08E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.97|P-value:8.18E-4 ID:SGTC_1932|Compound:st074806|FD-Score:5.88|P-value:1.04E-6 ID:SGTC_2173|Compound:5763948|FD-Score:4.36|P-value:2.53E-4 ID:SGTC_2192|Compound:5915882|FD-Score:5.87|P-value:1.10E-6 ID:SGTC_2342|Compound:7990637|FD-Score:4.75|P-value:7.13E-5 ID:SGTC_2393|Compound:9031399|FD-Score:4.47|P-value:1.82E-4 ID:SGTC_2460|Compound:5552655|FD-Score:5.29|P-value:1.05E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:-4.07|P-value:1.88E-4 ID:SGTC_2692|Compound:st077426|FD-Score:4.49|P-value:1.69E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-5.01|P-value:7.06E-6 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.58|P-value:1.27E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.65|P-value:2.66E-5 ID:SGTC_2818|Compound:7996756|FD-Score:4.63|P-value:1.07E-4 ID:SGTC_2916|Compound:7875993|FD-Score:4.07|P-value:6.16E-4 ID:SGTC_2970|Compound:9090938|FD-Score:6.96|P-value:8.32E-9 ID:SGTC_3052|Compound:9092717|FD-Score:4.41|P-value:2.19E-4 ID:SGTC_3056|Compound:9094900|FD-Score:-4.39|P-value:6.59E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-3.56|P-value:8.71E-4 ID:SGTC_3127|Compound:9126946|FD-Score:-3.92|P-value:3.02E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.74|P-value:7.43E-5 ID:SGTC_3233|Compound:9132340|FD-Score:3.99|P-value:7.81E-4 ID:SGTC_3286|Compound:9117135|FD-Score:4.54|P-value:1.46E-4 ID:SGTC_3306|Compound:9127676|FD-Score:6.59|P-value:4.71E-8 ID:SGTC_3310|Compound:9130229|FD-Score:4.15|P-value:4.90E-4 ID:SGTC_3337|Compound:9144342|FD-Score:6.90|P-value:1.12E-8 ID:SGTC_3341|Compound:9147222|FD-Score:9.28|P-value:1.73E-14

Top fitness defect scores for YDR280W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 16.80 4.6 µM 8.56E-44 heat shock/prefoldin 3 2.02E-63 5.16
2 SGTC_610 4215-0184 12.90 155.0 µM 1.16E-26 exosome 2 1.56E-38 1.72
3 SGTC_1074 carmofur 12.60 3.8 µM 1.90E-25 exosome 4 9.13E-37 2.30
4 SGTC_261 5-fluorouridine 12.00 15.4 µM 3.88E-23 exosome 1 2.10E-33 4.60
5 SGTC_1077 5-fluorocytosine 11.80 377.0 nM 2.00E-22 exosome 3 2.29E-32 1.07
6 SGTC_1261 0712-0010 9.37 12.7 µM 9.65E-15 RNA processing & uracil transport 1 3.61E-21 3.00
7 SGTC_3341 9147222 9.28 25.9 µM 1.73E-14 RNA processing & uracil transport 1 8.43E-21 2.09
8 SGTC_371 0195-0006 8.80 7.4 µM 3.49E-13 2 6.79E-19 2.51
9 SGTC_1698 5-fluorocytosine 8.58 1.1 µM 1.30E-12 exosome 4 4.61E-18 0.16
10 SGTC_923 1900-2434 7.05 73.3 µM 5.40E-9 RNA processing & uracil transport 1 9.07E-13 1.10
11 SGTC_832 4083-0019 7.02 164.0 µM 6.16E-9 translation 5 1.10E-12 0.05
12 SGTC_2970 9090938 6.96 41.9 µM 8.32E-9 RNA processing & uracil transport 2 1.71E-12 1.26
13 SGTC_3337 9144342 6.90 33.5 µM 1.12E-8 5 2.63E-12 1.40
14 SGTC_118 0083-0097 6.77 135.3 µM 2.05E-8 translation 2 6.38E-12 0.21
15 SGTC_3306 9127676 6.59 59.8 µM 4.71E-8 1 2.16E-11 2.01
16 SGTC_617 3770-0098 6.21 35.0 µM 2.66E-7 exosome 15 2.73E-10 0.36
17 SGTC_423 5-fluorouracil 6.18 457.0 µM 2.99E-7 exosome 11 3.24E-10 0.09
18 SGTC_1300 1094-0077 6.03 113.0 µM 5.56E-7 1 8.04E-10 1.12
19 SGTC_1932 st074806 5.88 40.9 µM 1.04E-6 4 2.02E-9 0.67
20 SGTC_2192 5915882 5.87 198.5 µM 1.10E-6 RNA processing & uracil transport 8 2.18E-9 0.69

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.485 3.15E-197 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.445 9.76E-163 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.433 2.34E-153 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.385 4.74E-119 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.341 9.12E-92 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.305 6.17E-73 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.300 7.82E-71 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.290 7.18E-66 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.282 4.08E-62 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.279 6.10E-61 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.262 1.80E-53 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.209 2.53E-34 YNL061W NOP2 het Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.207 1.04E-33 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.178 4.16E-25 YNL110C NOP15 het Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
0.164 1.24E-21 YOR206W NOC2 het Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors