HIPHOP chemogenomics database

YDR280W / RRP45

Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDR280W / RRP45

Click on Significant Values for Screen Details

Top fitness defect scores for YDR280W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	16.80	4.6 µM	8.56E-44	heat shock/prefoldin	3	2.02E-63	5.16
2	SGTC_610	4215-0184	12.90	155.0 µM	1.16E-26	exosome	2	1.56E-38	1.72
3	SGTC_1074	carmofur	12.60	3.8 µM	1.90E-25	exosome	4	9.13E-37	2.30
4	SGTC_261	5-fluorouridine	12.00	15.4 µM	3.88E-23	exosome	1	2.10E-33	4.60
5	SGTC_1077	5-fluorocytosine	11.80	377.0 nM	2.00E-22	exosome	3	2.29E-32	1.07
6	SGTC_1261	0712-0010	9.37	12.7 µM	9.65E-15	RNA processing & uracil transport	1	3.61E-21	3.00
7	SGTC_3341	9147222	9.28	25.9 µM	1.73E-14	RNA processing & uracil transport	1	8.43E-21	2.09
8	SGTC_371	0195-0006	8.80	7.4 µM	3.49E-13		2	6.79E-19	2.51
9	SGTC_1698	5-fluorocytosine	8.58	1.1 µM	1.30E-12	exosome	4	4.61E-18	0.16
10	SGTC_923	1900-2434	7.05	73.3 µM	5.40E-9	RNA processing & uracil transport	1	9.07E-13	1.10
11	SGTC_832	4083-0019	7.02	164.0 µM	6.16E-9	translation	5	1.10E-12	0.05
12	SGTC_2970	9090938	6.96	41.9 µM	8.32E-9	RNA processing & uracil transport	2	1.71E-12	1.26
13	SGTC_3337	9144342	6.90	33.5 µM	1.12E-8		5	2.63E-12	1.40
14	SGTC_118	0083-0097	6.77	135.3 µM	2.05E-8	translation	2	6.38E-12	0.21
15	SGTC_3306	9127676	6.59	59.8 µM	4.71E-8		1	2.16E-11	2.01
16	SGTC_617	3770-0098	6.21	35.0 µM	2.66E-7	exosome	15	2.73E-10	0.36
17	SGTC_423	5-fluorouracil	6.18	457.0 µM	2.99E-7	exosome	11	3.24E-10	0.09
18	SGTC_1300	1094-0077	6.03	113.0 µM	5.56E-7		1	8.04E-10	1.12
19	SGTC_1932	st074806	5.88	40.9 µM	1.04E-6		4	2.02E-9	0.67
20	SGTC_2192	5915882	5.87	198.5 µM	1.10E-6	RNA processing & uracil transport	8	2.18E-9	0.69

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.485	3.15E-197	YGR195W	SKI6	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.445	9.76E-163	YOL021C	DIS3	het	Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.433	2.34E-153	YDL111C	RRP42	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.385	4.74E-119	YGR158C	MTR3	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.341	9.12E-92	YPL043W	NOP4	het	Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.305	6.17E-73	YGR095C	RRP46	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.300	7.82E-71	YPL044C_d	YPL044C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.290	7.18E-66	YKL172W	EBP2	het	Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.282	4.08E-62	YNL002C	RLP7	het	Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.279	6.10E-61	YPR142C_d	YPR142C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.262	1.80E-53	YPR143W	RRP15	het	Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.209	2.53E-34	YNL061W	NOP2	het	Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.207	1.04E-33	YNL232W	CSL4	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.178	4.16E-25	YNL110C	NOP15	het	Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
0.164	1.24E-21	YOR206W	NOC2	het	Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors