YDR288W / NSE3

Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR288W / NSE3

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:5.27|P-value:2.02E-6 ID:SGTC_85|Compound:0868-0259|FD-Score:3.90|P-value:3.30E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.93|P-value:7.14E-6 ID:SGTC_198|Compound:3454-2873|FD-Score:3.64|P-value:7.26E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.64|P-value:2.44E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:-3.54|P-value:9.01E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.62|P-value:7.85E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.56|P-value:9.36E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:5.59|P-value:4.99E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.54|P-value:9.78E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:5.25|P-value:2.17E-6 ID:SGTC_1133|Compound:3473-0247|FD-Score:-4.20|P-value:1.08E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.70|P-value:6.17E-4 ID:SGTC_1522|Compound:st012842|FD-Score:4.90|P-value:8.88E-6 ID:SGTC_1661|Compound:st013063|FD-Score:-3.88|P-value:3.20E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.31|P-value:8.22E-5 ID:SGTC_2272|Compound:7805186|FD-Score:-4.24|P-value:9.48E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-3.93|P-value:2.72E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-3.57|P-value:8.33E-4 ID:SGTC_2447|Compound:5741742|FD-Score:5.36|P-value:1.37E-6 ID:SGTC_2448|Compound:5185112|FD-Score:6.16|P-value:3.51E-8 ID:SGTC_2449|Compound:5812982|FD-Score:-3.68|P-value:6.00E-4 ID:SGTC_2460|Compound:5552655|FD-Score:5.62|P-value:4.28E-7 ID:SGTC_2492|Compound:derrusnin|FD-Score:-3.86|P-value:3.39E-4 ID:SGTC_2534|Compound:helenine|FD-Score:-7.55|P-value:1.63E-11 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-5.68|P-value:2.99E-7 ID:SGTC_2545|Compound:solidagenone|FD-Score:-3.51|P-value:9.87E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.69|P-value:6.37E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.89|P-value:9.55E-6 ID:SGTC_2726|Compound:cimetidine|FD-Score:3.69|P-value:6.29E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.69|P-value:5.88E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.86|P-value:3.72E-4 ID:SGTC_2959|Compound:9079155|FD-Score:6.43|P-value:9.07E-9 ID:SGTC_2980|Compound:9014342|FD-Score:-4.95|P-value:6.69E-6 ID:SGTC_2984|Compound:9022109|FD-Score:3.70|P-value:6.06E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-4.46|P-value:4.42E-5 ID:SGTC_3078|Compound:9116413|FD-Score:-4.80|P-value:1.23E-5 ID:SGTC_3129|Compound:9127257|FD-Score:4.82|P-value:1.23E-5 ID:SGTC_3137|Compound:9097352|FD-Score:-4.21|P-value:1.05E-4 ID:SGTC_3138|Compound:9097216|FD-Score:4.18|P-value:1.30E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-4.43|P-value:4.92E-5 ID:SGTC_3193|Compound:9111931|FD-Score:3.69|P-value:6.28E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.61|P-value:7.91E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-5.74|P-value:2.27E-7 ID:SGTC_3333|Compound:9141820|FD-Score:3.84|P-value:3.98E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:5.27|P-value:2.02E-6 ID:SGTC_85|Compound:0868-0259|FD-Score:3.90|P-value:3.30E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.93|P-value:7.14E-6 ID:SGTC_198|Compound:3454-2873|FD-Score:3.64|P-value:7.26E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.64|P-value:2.44E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:-3.54|P-value:9.01E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.62|P-value:7.85E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.56|P-value:9.36E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:5.59|P-value:4.99E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.54|P-value:9.78E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:5.25|P-value:2.17E-6 ID:SGTC_1133|Compound:3473-0247|FD-Score:-4.20|P-value:1.08E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.70|P-value:6.17E-4 ID:SGTC_1522|Compound:st012842|FD-Score:4.90|P-value:8.88E-6 ID:SGTC_1661|Compound:st013063|FD-Score:-3.88|P-value:3.20E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.31|P-value:8.22E-5 ID:SGTC_2272|Compound:7805186|FD-Score:-4.24|P-value:9.48E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-3.93|P-value:2.72E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-3.57|P-value:8.33E-4 ID:SGTC_2447|Compound:5741742|FD-Score:5.36|P-value:1.37E-6 ID:SGTC_2448|Compound:5185112|FD-Score:6.16|P-value:3.51E-8 ID:SGTC_2449|Compound:5812982|FD-Score:-3.68|P-value:6.00E-4 ID:SGTC_2460|Compound:5552655|FD-Score:5.62|P-value:4.28E-7 ID:SGTC_2492|Compound:derrusnin|FD-Score:-3.86|P-value:3.39E-4 ID:SGTC_2534|Compound:helenine|FD-Score:-7.55|P-value:1.63E-11 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-5.68|P-value:2.99E-7 ID:SGTC_2545|Compound:solidagenone|FD-Score:-3.51|P-value:9.87E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.69|P-value:6.37E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.89|P-value:9.55E-6 ID:SGTC_2726|Compound:cimetidine|FD-Score:3.69|P-value:6.29E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.69|P-value:5.88E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.86|P-value:3.72E-4 ID:SGTC_2959|Compound:9079155|FD-Score:6.43|P-value:9.07E-9 ID:SGTC_2980|Compound:9014342|FD-Score:-4.95|P-value:6.69E-6 ID:SGTC_2984|Compound:9022109|FD-Score:3.70|P-value:6.06E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-4.46|P-value:4.42E-5 ID:SGTC_3078|Compound:9116413|FD-Score:-4.80|P-value:1.23E-5 ID:SGTC_3129|Compound:9127257|FD-Score:4.82|P-value:1.23E-5 ID:SGTC_3137|Compound:9097352|FD-Score:-4.21|P-value:1.05E-4 ID:SGTC_3138|Compound:9097216|FD-Score:4.18|P-value:1.30E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-4.43|P-value:4.92E-5 ID:SGTC_3193|Compound:9111931|FD-Score:3.69|P-value:6.28E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.61|P-value:7.91E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-5.74|P-value:2.27E-7 ID:SGTC_3333|Compound:9141820|FD-Score:3.84|P-value:3.98E-4

Top fitness defect scores for YDR288W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2959 9079155 6.43 11.0 µM 9.07E-9 2 6.41E-11 0.47
2 SGTC_2448 5185112 6.16 27.2 µM 3.51E-8 2 3.68E-10 0.33
3 SGTC_2460 5552655 5.62 174.2 µM 4.28E-7 amide catabolism 2 9.34E-9 0.33
4 SGTC_913 3487-1245 5.59 167.0 µM 4.99E-7 4 1.14E-8 0.59
5 SGTC_2447 5741742 5.36 33.8 µM 1.37E-6 2 4.18E-8 0.13
6 SGTC_52 0329-0193 5.27 875.0 nM 2.02E-6 TSC3-RPN4 3 6.92E-8 0.38
7 SGTC_1048 3161-0300 5.25 24.5 µM 2.17E-6 3 7.59E-8 0.81
8 SGTC_1522 st012842 4.90 45.6 µM 8.88E-6 RNA pol III & RNase P/MRP 7 4.68E-7 0.07
9 SGTC_2638 epoxomicin 4.89 18.0 µM 9.55E-6 2 5.14E-7 0.82
10 SGTC_3129 9127257 4.82 49.5 µM 1.23E-5 2 7.10E-7 0.48
11 SGTC_451 chloral hydrate 4.64 100.0 µM 2.44E-5 11 1.72E-6 0.02
12 SGTC_1745 st081495 4.31 74.0 µM 8.22E-5 RNA processing & uracil transport 28 8.22E-6 0.02
13 SGTC_3138 9097216 4.18 49.5 µM 1.30E-4 3 1.48E-5 0.07
14 SGTC_85 0868-0259 3.90 160.1 µM 3.30E-4 60S ribosome export 7 4.91E-5 0.30
15 SGTC_2837 9008476 3.86 45.5 µM 3.72E-4 6 5.71E-5 2.28E-4
16 SGTC_3333 9141820 3.84 12.1 µM 3.98E-4 5 6.25E-5 0.01
17 SGTC_2984 9022109 3.70 39.0 µM 6.06E-4 3 1.07E-4 0.06
18 SGTC_1181 1222-0449 3.70 220.0 µM 6.17E-4 4 1.09E-4 0.23
19 SGTC_3193 9111931 3.69 49.5 µM 6.28E-4 12 1.12E-4 0.19
20 SGTC_2726 cimetidine 3.69 79.3 µM 6.29E-4 4 1.12E-4 0.63

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.089 2.24E-7 YIL145C PAN6 hom Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
0.089 2.74E-7 YDR183W PLP1 hom Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
0.089 2.80E-7 YNR053C NOG2 het Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
0.085 8.80E-7 YPL114W_d YPL114W_d hom Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
0.079 4.55E-6 YCL064C CHA1 hom Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine
0.076 1.15E-5 YER043C SAH1 het S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.075 1.40E-5 YEL033W_p MTC7_p hom Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
0.074 1.85E-5 YAR029W_p YAR029W_p hom Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.073 2.27E-5 YDL154W MSH5 hom Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
0.073 2.49E-5 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.073 2.56E-5 YJR005C-A_p YJR005C-A_p hom Putative protein of unknown function; originally identified as a syntenic homolog of an Ashbya gossypii gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication
0.070 4.47E-5 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.069 5.96E-5 YLR435W TSR2 hom Protein with a potential role in pre-rRNA processing
0.069 6.67E-5 YGR091W PRP31 het Splicing factor, component of the U4/U6-U5 snRNP complex
0.069 7.06E-5 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay