YDR291W / HRQ1

3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS)

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR291W / HRQ1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR291W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2677 mitomycin C 6.35 100.0 µM 1.27E-11 DNA damage response 4 1.08E-10
2 SGTC_539 4859-0462 4.97 2.5 µM 8.94E-8 RNA pol III & RNase P/MRP 6 3.38E-7
3 SGTC_2769 toremifene 4.74 3.7 µM 3.09E-7 10 1.04E-6
4 SGTC_2053 5265679 4.59 138.0 µM 7.02E-7 11 2.20E-6
5 SGTC_542 3381-0244 4.18 89.3 µM 5.53E-6 34 1.44E-5
6 SGTC_2614 arthonioic acid 3.96 13.4 µM 1.60E-5 22 3.77E-5
7 SGTC_1522 st012842 3.94 45.6 µM 1.75E-5 RNA pol III & RNase P/MRP 26 4.09E-5
8 SGTC_331 k048-0133 3.93 24.5 µM 1.79E-5 RSC & ERG11 10 4.19E-5
9 SGTC_2282 7944268 3.86 200.0 µM 2.52E-5 RPP1 & pyrimidine depletion 31 5.69E-5
10 SGTC_2286 7945697 3.78 125.0 µM 3.64E-5 64 7.97E-5
11 SGTC_1581 himbacine 3.74 57.9 µM 4.21E-5 Golgi 54 9.10E-5
12 SGTC_2586 curcumin 3.72 80.0 µM 4.66E-5 iron homeostasis 61 9.98E-5
13 SGTC_1372 2752-0194 3.69 270.0 µM 5.27E-5 25 1.12E-4
14 SGTC_2607 solasodine 3.67 47.4 µM 5.68E-5 68 1.20E-4
15 SGTC_2675 theaflavin digallate 3.63 100.0 µM 6.83E-5 iron homeostasis 39 1.41E-4
16 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 3.52 54.9 µM 1.08E-4 redox potentiating 71 2.15E-4
17 SGTC_1230 0170-0365 3.40 3.8 µM 1.76E-4 endomembrane recycling 99 3.35E-4
18 SGTC_2009 4012297 3.37 12.1 µM 2.01E-4 cell wall signaling 89 3.78E-4
19 SGTC_335 4077-0256 3.37 27.6 µM 2.02E-4 29 3.79E-4
20 SGTC_3212 9128307 3.35 49.5 µM 2.19E-4 76 4.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.288 1.39E-64 YDL010W GRX6 hom Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
0.223 1.10E-38 YOR092W ECM3 hom Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
0.204 1.27E-32 YCR007C_p YCR007C_p hom Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene
0.196 5.22E-30 YBL065W_d YBL065W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene
0.160 1.86E-20 YLR244C MAP1 hom Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
0.149 4.92E-18 YOR234C RPL33B hom Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
0.135 4.85E-15 YDR314C RAD34 hom Protein involved in nucleotide excision repair (NER); homologous to RAD4
0.132 1.97E-14 YMR114C YMR114C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
0.129 6.79E-14 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.129 8.58E-14 YDR403W DIT1 hom Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
0.128 1.11E-13 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.128 1.30E-13 YOL102C TPT1 het tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.126 2.60E-13 YGR295C COS6 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.115 2.35E-11 YJL010C NOP9 het Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
0.112 9.06E-11 YBR196C-A_p YBR196C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR