YDR299W / BFR2

Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR299W / BFR2

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-5.41|P-value:1.30E-10 ID:SGTC_156|Compound:3474-0145|FD-Score:2.92|P-value:2.87E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:-2.80|P-value:5.47E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:2.66|P-value:8.67E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:-2.76|P-value:6.39E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:2.71|P-value:7.17E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:2.88|P-value:3.48E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-5.77|P-value:7.87E-12 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.45|P-value:2.85E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:-2.71|P-value:8.01E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:-2.98|P-value:2.50E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.22|P-value:8.55E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.00|P-value:2.32E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:2.86|P-value:3.83E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:2.65|P-value:8.92E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:-2.80|P-value:5.37E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.71|P-value:7.80E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.01|P-value:2.27E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.65|P-value:9.04E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.82|P-value:3.60E-6 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.19|P-value:1.00E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:2.93|P-value:2.78E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:-2.81|P-value:5.30E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:2.67|P-value:8.25E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.42|P-value:2.85E-5 ID:SGTC_1471|Compound:k673-0312|FD-Score:-3.40|P-value:3.64E-5 ID:SGTC_1478|Compound:0453-0656|FD-Score:-3.18|P-value:1.02E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.06|P-value:9.05E-7 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:2.92|P-value:2.91E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.31|P-value:4.83E-5 ID:SGTC_1611|Compound:st001856|FD-Score:2.64|P-value:9.23E-4 ID:SGTC_1612|Compound:st002233|FD-Score:-3.18|P-value:1.02E-4 ID:SGTC_1630|Compound:st005209|FD-Score:2.78|P-value:5.26E-4 ID:SGTC_1636|Compound:st007707|FD-Score:2.77|P-value:5.49E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-2.79|P-value:5.59E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:2.70|P-value:7.31E-4 ID:SGTC_1714|Compound:st033236|FD-Score:2.83|P-value:4.27E-4 ID:SGTC_1988|Compound:st071872|FD-Score:3.08|P-value:1.47E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.84|P-value:4.06E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-3.00|P-value:2.35E-4 ID:SGTC_2064|Compound:5220758|FD-Score:2.91|P-value:3.00E-4 ID:SGTC_2076|Compound:5212012|FD-Score:2.93|P-value:2.77E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.63|P-value:9.78E-6 ID:SGTC_2143|Compound:5623677|FD-Score:2.78|P-value:5.37E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.10|P-value:1.51E-4 ID:SGTC_2272|Compound:7805186|FD-Score:2.67|P-value:8.25E-4 ID:SGTC_2301|Compound:7769476|FD-Score:2.95|P-value:2.53E-4 ID:SGTC_2386|Compound:9075165|FD-Score:3.57|P-value:1.32E-5 ID:SGTC_2421|Compound:5690543|FD-Score:3.03|P-value:1.79E-4 ID:SGTC_2478|Compound:5763493|FD-Score:3.08|P-value:1.45E-4 ID:SGTC_2481|Compound:5478948|FD-Score:-2.85|P-value:4.50E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:7.41|P-value:1.67E-18 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.00|P-value:2.12E-9 ID:SGTC_2784|Compound:7567673|FD-Score:-3.11|P-value:1.40E-4 ID:SGTC_2789|Compound:5281112|FD-Score:-3.02|P-value:2.18E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.41|P-value:3.04E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.58|P-value:1.26E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.12|P-value:1.34E-4 ID:SGTC_2851|Compound:9015922|FD-Score:5.60|P-value:2.32E-11 ID:SGTC_2855|Compound:9016525|FD-Score:-3.23|P-value:8.34E-5 ID:SGTC_2857|Compound:9024691|FD-Score:5.26|P-value:3.15E-10 ID:SGTC_2959|Compound:9079155|FD-Score:3.55|P-value:1.49E-5 ID:SGTC_3155|Compound:9099265|FD-Score:3.01|P-value:1.99E-4 ID:SGTC_3337|Compound:9144342|FD-Score:3.53|P-value:1.61E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-5.41|P-value:1.30E-10 ID:SGTC_156|Compound:3474-0145|FD-Score:2.92|P-value:2.87E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:-2.80|P-value:5.47E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:2.66|P-value:8.67E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:-2.76|P-value:6.39E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:2.71|P-value:7.17E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:2.88|P-value:3.48E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-5.77|P-value:7.87E-12 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.45|P-value:2.85E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:-2.71|P-value:8.01E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:-2.98|P-value:2.50E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.22|P-value:8.55E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.00|P-value:2.32E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:2.86|P-value:3.83E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:2.65|P-value:8.92E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:-2.80|P-value:5.37E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.71|P-value:7.80E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.01|P-value:2.27E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.65|P-value:9.04E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.82|P-value:3.60E-6 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.19|P-value:1.00E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:2.93|P-value:2.78E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:-2.81|P-value:5.30E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:2.67|P-value:8.25E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.42|P-value:2.85E-5 ID:SGTC_1471|Compound:k673-0312|FD-Score:-3.40|P-value:3.64E-5 ID:SGTC_1478|Compound:0453-0656|FD-Score:-3.18|P-value:1.02E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.06|P-value:9.05E-7 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:2.92|P-value:2.91E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.31|P-value:4.83E-5 ID:SGTC_1611|Compound:st001856|FD-Score:2.64|P-value:9.23E-4 ID:SGTC_1612|Compound:st002233|FD-Score:-3.18|P-value:1.02E-4 ID:SGTC_1630|Compound:st005209|FD-Score:2.78|P-value:5.26E-4 ID:SGTC_1636|Compound:st007707|FD-Score:2.77|P-value:5.49E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-2.79|P-value:5.59E-4 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:2.70|P-value:7.31E-4 ID:SGTC_1714|Compound:st033236|FD-Score:2.83|P-value:4.27E-4 ID:SGTC_1988|Compound:st071872|FD-Score:3.08|P-value:1.47E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.84|P-value:4.06E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-3.00|P-value:2.35E-4 ID:SGTC_2064|Compound:5220758|FD-Score:2.91|P-value:3.00E-4 ID:SGTC_2076|Compound:5212012|FD-Score:2.93|P-value:2.77E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.63|P-value:9.78E-6 ID:SGTC_2143|Compound:5623677|FD-Score:2.78|P-value:5.37E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.10|P-value:1.51E-4 ID:SGTC_2272|Compound:7805186|FD-Score:2.67|P-value:8.25E-4 ID:SGTC_2301|Compound:7769476|FD-Score:2.95|P-value:2.53E-4 ID:SGTC_2386|Compound:9075165|FD-Score:3.57|P-value:1.32E-5 ID:SGTC_2421|Compound:5690543|FD-Score:3.03|P-value:1.79E-4 ID:SGTC_2478|Compound:5763493|FD-Score:3.08|P-value:1.45E-4 ID:SGTC_2481|Compound:5478948|FD-Score:-2.85|P-value:4.50E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:7.41|P-value:1.67E-18 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.00|P-value:2.12E-9 ID:SGTC_2784|Compound:7567673|FD-Score:-3.11|P-value:1.40E-4 ID:SGTC_2789|Compound:5281112|FD-Score:-3.02|P-value:2.18E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.41|P-value:3.04E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.58|P-value:1.26E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.12|P-value:1.34E-4 ID:SGTC_2851|Compound:9015922|FD-Score:5.60|P-value:2.32E-11 ID:SGTC_2855|Compound:9016525|FD-Score:-3.23|P-value:8.34E-5 ID:SGTC_2857|Compound:9024691|FD-Score:5.26|P-value:3.15E-10 ID:SGTC_2959|Compound:9079155|FD-Score:3.55|P-value:1.49E-5 ID:SGTC_3155|Compound:9099265|FD-Score:3.01|P-value:1.99E-4 ID:SGTC_3337|Compound:9144342|FD-Score:3.53|P-value:1.61E-5

Top fitness defect scores for YDR299W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2563 tomatine 7.41 8.5 µM 1.67E-18 2 6.36E-14 0.87
2 SGTC_2851 9015922 5.60 71.4 µM 2.32E-11 3 1.06E-8 0.21
3 SGTC_2857 9024691 5.26 58.4 µM 3.15E-10 1 7.13E-8 1.03
4 SGTC_2564 betulinic acid 5.00 100.0 µM 2.12E-9 6 2.89E-7 0.60
5 SGTC_1514 1189-1591 4.06 158.0 µM 9.05E-7 5 2.46E-5 0.24
6 SGTC_1132 3456-3614 3.82 2.5 µM 3.60E-6 tubulin folding & SWR complex 11 6.80E-5 0.49
7 SGTC_2109 5483026 3.63 200.0 µM 9.78E-6 RSC complex & mRNA processing 38 1.42E-4 0.01
8 SGTC_2812 7970281 3.58 58.4 µM 1.26E-5 14 1.71E-4 0.08
9 SGTC_2386 9075165 3.57 157.4 µM 1.32E-5 3 1.77E-4 0.20
10 SGTC_2959 9079155 3.55 11.0 µM 1.49E-5 15 1.94E-4 0.07
11 SGTC_3337 9144342 3.53 33.5 µM 1.61E-5 16 2.05E-4 0.21
12 SGTC_1401 3807-4679 3.42 7.6 µM 2.85E-5 tubulin folding & SWR complex 11 3.12E-4 0.15
13 SGTC_2808 7946468 3.41 45.5 µM 3.04E-5 14 3.28E-4 6.30E-4
14 SGTC_1569 carminic acid 3.31 40.6 µM 4.83E-5 5 4.62E-4 0.31
15 SGTC_2478 5763493 3.08 32.3 µM 1.45E-4 13 0.00104 0.08
16 SGTC_1988 st071872 3.08 53.4 µM 1.47E-4 2 0.00105 0.03
17 SGTC_2421 5690543 3.03 90.7 µM 1.79E-4 11 0.00122 0.05
18 SGTC_3155 9099265 3.01 49.5 µM 1.99E-4 21 0.00132 0.07
19 SGTC_2301 7769476 2.95 200.0 µM 2.53E-4 25 0.00157 0.02
20 SGTC_2076 5212012 2.93 200.0 µM 2.77E-4 7 0.00168 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.187 1.14E-27 YPR013C_p CMR3_p hom Putative zinc finger protein; YPR013C is not an essential gene
0.172 8.57E-24 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.148 6.63E-18 YBL063W KIP1 hom Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
0.132 1.74E-14 YDR018C_p YDR018C_p hom Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication
0.129 6.98E-14 YGR153W_p YGR153W_p hom Putative protein of unknown function
0.127 1.31E-13 YEL038W UTR4 hom Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus
0.121 2.36E-12 YBL055C YBL055C hom 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
0.119 4.75E-12 YNL212W VID27 hom Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
0.113 4.61E-11 YDL008W APC11 het Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
0.110 1.60E-10 YLR077W FMP25 hom Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
0.109 2.41E-10 YGL109W_d YGL109W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C
0.108 3.49E-10 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.106 8.75E-10 YBR070C ALG14 het Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
0.103 2.47E-9 YBR085W AAC3 hom Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
0.101 4.83E-9 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress