YDR301W / CFT1

RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR301W / CFT1

Click on Significant Values for Screen Details ID:SGTC_121|Compound:0557-0495|FD-Score:3.20|P-value:7.59E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:3.27|P-value:5.85E-4 ID:SGTC_270|Compound:tamoxifen|FD-Score:4.42|P-value:6.03E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:3.46|P-value:3.01E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:5.04|P-value:2.95E-7 ID:SGTC_528|Compound:3492-0019|FD-Score:4.19|P-value:1.61E-5 ID:SGTC_534|Compound:k292-0788|FD-Score:6.95|P-value:2.99E-12 ID:SGTC_706|Compound:4262-0057|FD-Score:3.21|P-value:7.26E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.38|P-value:3.99E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.62|P-value:1.69E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:4.34|P-value:8.67E-6 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.89|P-value:6.08E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:-5.38|P-value:5.48E-8 ID:SGTC_1041|Compound:0206-0046|FD-Score:-3.42|P-value:3.65E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.16|P-value:8.75E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.60|P-value:1.83E-4 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.10|P-value:2.43E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.24|P-value:6.81E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.17|P-value:8.74E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.14|P-value:9.35E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.41|P-value:3.62E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.48|P-value:4.44E-6 ID:SGTC_1362|Compound:1611-0397|FD-Score:3.38|P-value:4.03E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.84|P-value:7.08E-5 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.44|P-value:3.27E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-3.78|P-value:9.49E-5 ID:SGTC_1662|Compound:st014054|FD-Score:-4.13|P-value:2.26E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.50|P-value:2.58E-8 ID:SGTC_1733|Compound:st037357|FD-Score:3.61|P-value:1.70E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.28|P-value:1.09E-5 ID:SGTC_1922|Compound:5578187|FD-Score:6.63|P-value:2.59E-11 ID:SGTC_2010|Compound:4012422|FD-Score:5.30|P-value:7.39E-8 ID:SGTC_2036|Compound:5160441|FD-Score:5.26|P-value:9.30E-8 ID:SGTC_2038|Compound:4100038|FD-Score:5.34|P-value:6.04E-8 ID:SGTC_2245|Compound:6637752|FD-Score:4.09|P-value:2.53E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.21|P-value:7.37E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.14|P-value:9.60E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.53|P-value:2.31E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.52|P-value:2.53E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:4.60|P-value:2.58E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.44|P-value:3.48E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.96|P-value:4.59E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.30|P-value:5.37E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.87|P-value:6.24E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:-4.58|P-value:3.08E-6 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.16|P-value:8.73E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:-3.30|P-value:5.62E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-3.99|P-value:4.00E-5 ID:SGTC_2704|Compound:st078022|FD-Score:3.28|P-value:5.78E-4 ID:SGTC_2708|Compound:st078559|FD-Score:4.46|P-value:4.97E-6 ID:SGTC_2709|Compound:st078560|FD-Score:4.38|P-value:7.23E-6 ID:SGTC_2807|Compound:7945238|FD-Score:3.20|P-value:7.46E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.49|P-value:2.72E-4 ID:SGTC_2829|Compound:7992743|FD-Score:4.07|P-value:2.78E-5 ID:SGTC_2896|Compound:9052656|FD-Score:5.78|P-value:5.11E-9 ID:SGTC_2907|Compound:7721985|FD-Score:4.13|P-value:2.12E-5 ID:SGTC_2964|Compound:9083345|FD-Score:7.45|P-value:7.91E-14 ID:SGTC_2991|Compound:9074669|FD-Score:3.68|P-value:1.31E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.09|P-value:2.71E-5 ID:SGTC_3065|Compound:9113888|FD-Score:3.41|P-value:3.58E-4 ID:SGTC_3067|Compound:9117431|FD-Score:3.20|P-value:7.52E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.67|P-value:1.48E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.31|P-value:5.15E-4 ID:SGTC_3085|Compound:9117376|FD-Score:-5.67|P-value:1.07E-8 ID:SGTC_3093|Compound:9117344|FD-Score:4.71|P-value:1.53E-6 ID:SGTC_3097|Compound:9117246|FD-Score:-4.42|P-value:6.45E-6 ID:SGTC_3148|Compound:9097893|FD-Score:-3.66|P-value:1.50E-4 ID:SGTC_3155|Compound:9099265|FD-Score:3.84|P-value:7.03E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.34|P-value:4.63E-4 ID:SGTC_3263|Compound:9137018|FD-Score:4.77|P-value:1.17E-6 ID:SGTC_3274|Compound:9138298|FD-Score:3.74|P-value:1.03E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-4.32|P-value:9.80E-6 ID:SGTC_121|Compound:0557-0495|FD-Score:3.20|P-value:7.59E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:3.27|P-value:5.85E-4 ID:SGTC_270|Compound:tamoxifen|FD-Score:4.42|P-value:6.03E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:3.46|P-value:3.01E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:5.04|P-value:2.95E-7 ID:SGTC_528|Compound:3492-0019|FD-Score:4.19|P-value:1.61E-5 ID:SGTC_534|Compound:k292-0788|FD-Score:6.95|P-value:2.99E-12 ID:SGTC_706|Compound:4262-0057|FD-Score:3.21|P-value:7.26E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.38|P-value:3.99E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.62|P-value:1.69E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:4.34|P-value:8.67E-6 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.89|P-value:6.08E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:-5.38|P-value:5.48E-8 ID:SGTC_1041|Compound:0206-0046|FD-Score:-3.42|P-value:3.65E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.16|P-value:8.75E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.60|P-value:1.83E-4 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.10|P-value:2.43E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.24|P-value:6.81E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.17|P-value:8.74E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.14|P-value:9.35E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.41|P-value:3.62E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.48|P-value:4.44E-6 ID:SGTC_1362|Compound:1611-0397|FD-Score:3.38|P-value:4.03E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.84|P-value:7.08E-5 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.44|P-value:3.27E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-3.78|P-value:9.49E-5 ID:SGTC_1662|Compound:st014054|FD-Score:-4.13|P-value:2.26E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.50|P-value:2.58E-8 ID:SGTC_1733|Compound:st037357|FD-Score:3.61|P-value:1.70E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.28|P-value:1.09E-5 ID:SGTC_1922|Compound:5578187|FD-Score:6.63|P-value:2.59E-11 ID:SGTC_2010|Compound:4012422|FD-Score:5.30|P-value:7.39E-8 ID:SGTC_2036|Compound:5160441|FD-Score:5.26|P-value:9.30E-8 ID:SGTC_2038|Compound:4100038|FD-Score:5.34|P-value:6.04E-8 ID:SGTC_2245|Compound:6637752|FD-Score:4.09|P-value:2.53E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.21|P-value:7.37E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.14|P-value:9.60E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.53|P-value:2.31E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.52|P-value:2.53E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:4.60|P-value:2.58E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.44|P-value:3.48E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.96|P-value:4.59E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.30|P-value:5.37E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.87|P-value:6.24E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:-4.58|P-value:3.08E-6 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.16|P-value:8.73E-4 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:-3.30|P-value:5.62E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-3.99|P-value:4.00E-5 ID:SGTC_2704|Compound:st078022|FD-Score:3.28|P-value:5.78E-4 ID:SGTC_2708|Compound:st078559|FD-Score:4.46|P-value:4.97E-6 ID:SGTC_2709|Compound:st078560|FD-Score:4.38|P-value:7.23E-6 ID:SGTC_2807|Compound:7945238|FD-Score:3.20|P-value:7.46E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.49|P-value:2.72E-4 ID:SGTC_2829|Compound:7992743|FD-Score:4.07|P-value:2.78E-5 ID:SGTC_2896|Compound:9052656|FD-Score:5.78|P-value:5.11E-9 ID:SGTC_2907|Compound:7721985|FD-Score:4.13|P-value:2.12E-5 ID:SGTC_2964|Compound:9083345|FD-Score:7.45|P-value:7.91E-14 ID:SGTC_2991|Compound:9074669|FD-Score:3.68|P-value:1.31E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-4.09|P-value:2.71E-5 ID:SGTC_3065|Compound:9113888|FD-Score:3.41|P-value:3.58E-4 ID:SGTC_3067|Compound:9117431|FD-Score:3.20|P-value:7.52E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.67|P-value:1.48E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.31|P-value:5.15E-4 ID:SGTC_3085|Compound:9117376|FD-Score:-5.67|P-value:1.07E-8 ID:SGTC_3093|Compound:9117344|FD-Score:4.71|P-value:1.53E-6 ID:SGTC_3097|Compound:9117246|FD-Score:-4.42|P-value:6.45E-6 ID:SGTC_3148|Compound:9097893|FD-Score:-3.66|P-value:1.50E-4 ID:SGTC_3155|Compound:9099265|FD-Score:3.84|P-value:7.03E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.34|P-value:4.63E-4 ID:SGTC_3263|Compound:9137018|FD-Score:4.77|P-value:1.17E-6 ID:SGTC_3274|Compound:9138298|FD-Score:3.74|P-value:1.03E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-4.32|P-value:9.80E-6

Top fitness defect scores for YDR301W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2964 9083345 7.45 53.4 µM 7.91E-14 1 4.57E-14 0.89
2 SGTC_534 k292-0788 6.95 63.4 µM 2.99E-12 mitochondrial processes 1 1.86E-12 1.29
3 SGTC_1922 5578187 6.63 25.0 µM 2.59E-11 TRP & mitochondrial translation 3 1.68E-11 1.57
4 SGTC_2896 9052656 5.78 71.4 µM 5.11E-9 endomembrane recycling 12 3.69E-9 0.30
5 SGTC_1712 st032288 5.50 53.4 µM 2.58E-8 calcium & mitochondrial duress 16 1.93E-8 0.01
6 SGTC_2038 4100038 5.34 31.0 µM 6.04E-8 plasma membrane duress 7 4.59E-8 0.38
7 SGTC_2010 4012422 5.30 159.0 µM 7.39E-8 4 5.64E-8 0.74
8 SGTC_2036 5160441 5.26 105.0 µM 9.30E-8 NEO1 14 7.12E-8 0.10
9 SGTC_502 l-744,832 5.04 89.3 µM 2.95E-7 calcium & mitochondrial duress 4 2.31E-7 0.50
10 SGTC_3263 9137018 4.77 49.5 µM 1.17E-6 1 9.44E-7 0.87
11 SGTC_3093 9117344 4.71 49.5 µM 1.53E-6 60S ribosome export 3 1.24E-6 0.26
12 SGTC_2521 angolensin 4.60 13.4 µM 2.58E-6 1 2.11E-6 0.86
13 SGTC_1360 1598-0022 4.48 39.8 µM 4.44E-6 ERG2 2 3.67E-6 0.07
14 SGTC_2708 st078559 4.46 56.0 µM 4.97E-6 1 4.11E-6 0.60
15 SGTC_270 tamoxifen 4.42 5.5 µM 6.03E-6 NEO1 4 5.02E-6 0.41
16 SGTC_2709 st078560 4.38 56.0 µM 7.23E-6 4 6.03E-6 0.38
17 SGTC_1008 4106-0037 4.34 6.0 µM 8.67E-6 PDR1 4 7.26E-6 0.19
18 SGTC_1745 st081495 4.28 74.0 µM 1.09E-5 RNA processing & uracil transport 29 9.20E-6 0.01
19 SGTC_528 3492-0019 4.19 172.0 µM 1.61E-5 calcium & mitochondrial duress 18 1.37E-5 0.22
20 SGTC_2907 7721985 4.13 69.9 µM 2.12E-5 3 1.80E-5 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 7.10E-18 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.147 1.02E-17 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.129 7.44E-14 YCR008W SAT4 hom Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
0.124 5.09E-13 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.120 3.84E-12 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.116 1.42E-11 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.111 1.05E-10 YBR109C CMD1 het Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
0.111 1.10E-10 YBR172C SMY2 hom Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication
0.109 2.17E-10 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.107 4.68E-10 YML007C-A_p YML007C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
0.104 1.50E-9 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.103 1.90E-9 YDR053W_d YDR053W_d het Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
0.103 2.06E-9 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.101 4.37E-9 YOR360C PDE2 hom High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
0.098 1.29E-8 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria