YDR303C / RSC3

Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR303C / RSC3

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:-3.31|P-value:8.01E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.45|P-value:1.09E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.28|P-value:7.96E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:3.57|P-value:2.97E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.37|P-value:6.56E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:-3.92|P-value:9.07E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:5.57|P-value:4.47E-8 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.27|P-value:8.16E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.78|P-value:1.52E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:5.39|P-value:1.12E-7 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.33|P-value:6.87E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.37|P-value:5.89E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:4.56|P-value:5.91E-6 ID:SGTC_1673|Compound:st016611|FD-Score:4.35|P-value:1.43E-5 ID:SGTC_1753|Compound:st043925|FD-Score:-4.11|P-value:4.39E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-4.09|P-value:4.65E-5 ID:SGTC_1795|Compound:st049545|FD-Score:-3.27|P-value:9.10E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.38|P-value:6.35E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.40|P-value:5.38E-4 ID:SGTC_1963|Compound:st076565|FD-Score:-4.68|P-value:3.92E-6 ID:SGTC_2044|Compound:5102272|FD-Score:4.29|P-value:1.88E-5 ID:SGTC_2190|Compound:6049624|FD-Score:-3.92|P-value:8.97E-5 ID:SGTC_2191|Compound:5911152|FD-Score:-5.10|P-value:5.56E-7 ID:SGTC_2275|Compound:7947463|FD-Score:-3.58|P-value:3.12E-4 ID:SGTC_2336|Compound:9001819|FD-Score:7.91|P-value:1.59E-14 ID:SGTC_2344|Compound:7990960|FD-Score:5.04|P-value:6.40E-7 ID:SGTC_2387|Compound:9077560|FD-Score:-3.83|P-value:1.30E-4 ID:SGTC_2448|Compound:5185112|FD-Score:4.16|P-value:3.24E-5 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.47|P-value:4.28E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.71|P-value:1.79E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.86|P-value:2.33E-11 ID:SGTC_2835|Compound:9006588|FD-Score:-6.04|P-value:3.89E-9 ID:SGTC_2928|Compound:9011484|FD-Score:5.44|P-value:8.65E-8 ID:SGTC_3016|Compound:9080866|FD-Score:-4.15|P-value:3.70E-5 ID:SGTC_3017|Compound:9080681|FD-Score:-3.85|P-value:1.17E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-3.64|P-value:2.54E-4 ID:SGTC_3051|Compound:9093778|FD-Score:3.51|P-value:3.73E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:-5.33|P-value:1.80E-7 ID:SGTC_3124|Compound:9127567|FD-Score:3.58|P-value:2.84E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-3.83|P-value:1.27E-4 ID:SGTC_3228|Compound:9131799|FD-Score:-3.50|P-value:4.23E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.48|P-value:4.04E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-3.31|P-value:8.01E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.45|P-value:1.09E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.28|P-value:7.96E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:3.57|P-value:2.97E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.37|P-value:6.56E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:-3.92|P-value:9.07E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:5.57|P-value:4.47E-8 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.27|P-value:8.16E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.78|P-value:1.52E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:5.39|P-value:1.12E-7 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.33|P-value:6.87E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.37|P-value:5.89E-4 ID:SGTC_1437|Compound:4092-1038|FD-Score:4.56|P-value:5.91E-6 ID:SGTC_1673|Compound:st016611|FD-Score:4.35|P-value:1.43E-5 ID:SGTC_1753|Compound:st043925|FD-Score:-4.11|P-value:4.39E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-4.09|P-value:4.65E-5 ID:SGTC_1795|Compound:st049545|FD-Score:-3.27|P-value:9.10E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.38|P-value:6.35E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.40|P-value:5.38E-4 ID:SGTC_1963|Compound:st076565|FD-Score:-4.68|P-value:3.92E-6 ID:SGTC_2044|Compound:5102272|FD-Score:4.29|P-value:1.88E-5 ID:SGTC_2190|Compound:6049624|FD-Score:-3.92|P-value:8.97E-5 ID:SGTC_2191|Compound:5911152|FD-Score:-5.10|P-value:5.56E-7 ID:SGTC_2275|Compound:7947463|FD-Score:-3.58|P-value:3.12E-4 ID:SGTC_2336|Compound:9001819|FD-Score:7.91|P-value:1.59E-14 ID:SGTC_2344|Compound:7990960|FD-Score:5.04|P-value:6.40E-7 ID:SGTC_2387|Compound:9077560|FD-Score:-3.83|P-value:1.30E-4 ID:SGTC_2448|Compound:5185112|FD-Score:4.16|P-value:3.24E-5 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.47|P-value:4.28E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.71|P-value:1.79E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.86|P-value:2.33E-11 ID:SGTC_2835|Compound:9006588|FD-Score:-6.04|P-value:3.89E-9 ID:SGTC_2928|Compound:9011484|FD-Score:5.44|P-value:8.65E-8 ID:SGTC_3016|Compound:9080866|FD-Score:-4.15|P-value:3.70E-5 ID:SGTC_3017|Compound:9080681|FD-Score:-3.85|P-value:1.17E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-3.64|P-value:2.54E-4 ID:SGTC_3051|Compound:9093778|FD-Score:3.51|P-value:3.73E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:-5.33|P-value:1.80E-7 ID:SGTC_3124|Compound:9127567|FD-Score:3.58|P-value:2.84E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-3.83|P-value:1.27E-4 ID:SGTC_3228|Compound:9131799|FD-Score:-3.50|P-value:4.23E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.48|P-value:4.04E-4

Top fitness defect scores for YDR303C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2336 9001819 7.91 198.7 µM 1.59E-14 heme biosynthesis & mitochondrial translocase 17 1.29E-15 0.05
2 SGTC_2575 plumbagin 6.86 510.0 nM 2.33E-11 superoxide 8 3.55E-12 0.46
3 SGTC_902 1120-0002 5.57 2.1 µM 4.47E-8 azole & statin 9 1.29E-8 0.13
4 SGTC_2928 9011484 5.44 9.8 µM 8.65E-8 heme biosynthesis & mitochondrial translocase 17 2.65E-8 0.04
5 SGTC_1137 ixabepilone 5.39 100.0 µM 1.12E-7 3 3.49E-8 0.97
6 SGTC_2344 7990960 5.04 77.0 µM 6.40E-7 25 2.32E-7 0.02
7 SGTC_1437 4092-1038 4.56 11.6 µM 5.91E-6 1 2.57E-6 0.38
8 SGTC_1673 st016611 4.35 27.3 µM 1.43E-5 5 6.68E-6 0.38
9 SGTC_2044 5102272 4.29 200.0 µM 1.88E-5 6 9.00E-6 0.10
10 SGTC_2448 5185112 4.16 27.2 µM 3.24E-5 6 1.62E-5 0.17
11 SGTC_2566 crystal violet carbinol 3.71 226.0 nM 1.79E-4 23 1.03E-4 0.06
12 SGTC_3124 9127567 3.58 49.5 µM 2.84E-4 DNA damage response 9 1.70E-4 0.02
13 SGTC_327 1273-0057 3.57 4.0 µM 2.97E-4 azole & statin 11 1.78E-4 0.19
14 SGTC_3051 9093778 3.51 49.5 µM 3.73E-4 2 2.27E-4 0.22
15 SGTC_3278 9139605 3.48 49.5 µM 4.04E-4 14 2.48E-4 0.15
16 SGTC_2510 benzyl isothiocyanate 3.47 8.3 µM 4.28E-4 30 2.64E-4 0.03
17 SGTC_1926 methyl fluorone black 3.40 53.6 µM 5.38E-4 DNA intercalators 36 3.38E-4 0.16
18 SGTC_1371 2421-0008 3.37 32.7 µM 5.89E-4 mitochondrial processes 33 3.73E-4 0.14
19 SGTC_1310 1188-0297 3.33 994.0 nM 6.87E-4 azole & statin 19 4.40E-4 0.06
20 SGTC_120 rhodamine 6G 3.28 52.3 µM 7.96E-4 mitochondrial processes 27 5.15E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.245 8.46E-47 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.189 5.25E-28 YIL075C RPN2 het Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
0.184 1.13E-26 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.162 5.50E-21 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.131 3.60E-14 YGR029W ERV1 het Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
0.123 1.11E-12 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.122 1.39E-12 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.122 1.65E-12 YEL034W HYP2 het Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.116 2.19E-11 YKR008W RSC4 het Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
0.111 1.48E-10 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.105 1.43E-9 YDL196W_d YDL196W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
0.101 6.17E-9 YGL075C MPS2 het Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
0.097 1.87E-8 YIL126W STH1 het ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
0.096 2.76E-8 YJR049C UTR1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
0.095 4.01E-8 YPL062W_d YPL062W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation