YDR308C / SRB7

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR308C / SRB7

Click on Significant Values for Screen Details ID:SGTC_104|Compound:0074-0074|FD-Score:3.07|P-value:4.15E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:-3.07|P-value:4.08E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:-3.24|P-value:2.09E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.64|P-value:3.70E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.41|P-value:9.99E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:2.85|P-value:9.43E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:4.50|P-value:4.86E-7 ID:SGTC_882|Compound:0843-0131|FD-Score:3.54|P-value:5.74E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.08|P-value:4.01E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:3.60|P-value:4.35E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:5.67|P-value:3.20E-10 ID:SGTC_1143|Compound:3454-2408|FD-Score:2.95|P-value:6.58E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.09|P-value:3.80E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.21|P-value:2.33E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:5.42|P-value:1.77E-9 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.26|P-value:1.89E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.19|P-value:2.55E-4 ID:SGTC_1604|Compound:st002052|FD-Score:2.96|P-value:6.39E-4 ID:SGTC_1619|Compound:st003326|FD-Score:3.57|P-value:5.14E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.95|P-value:8.52E-6 ID:SGTC_1966|Compound:st070145|FD-Score:-2.95|P-value:6.52E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.54|P-value:5.72E-5 ID:SGTC_2010|Compound:4012422|FD-Score:2.91|P-value:7.67E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-2.88|P-value:8.58E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.64|P-value:3.62E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-3.03|P-value:4.86E-4 ID:SGTC_2432|Compound:5630864|FD-Score:2.96|P-value:6.34E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.76|P-value:2.07E-5 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.69|P-value:2.86E-5 ID:SGTC_2647|Compound:atorvastatin|FD-Score:3.10|P-value:3.62E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:2.84|P-value:9.96E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:-3.67|P-value:3.18E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-5.25|P-value:5.49E-9 ID:SGTC_2852|Compound:9023854|FD-Score:3.45|P-value:8.59E-5 ID:SGTC_2879|Compound:9041815|FD-Score:2.93|P-value:7.10E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:3.07|P-value:4.15E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:-3.07|P-value:4.08E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:-3.24|P-value:2.09E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.64|P-value:3.70E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.41|P-value:9.99E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:2.85|P-value:9.43E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:4.50|P-value:4.86E-7 ID:SGTC_882|Compound:0843-0131|FD-Score:3.54|P-value:5.74E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:-3.08|P-value:4.01E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:3.60|P-value:4.35E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:5.67|P-value:3.20E-10 ID:SGTC_1143|Compound:3454-2408|FD-Score:2.95|P-value:6.58E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.09|P-value:3.80E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.21|P-value:2.33E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:5.42|P-value:1.77E-9 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.26|P-value:1.89E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.19|P-value:2.55E-4 ID:SGTC_1604|Compound:st002052|FD-Score:2.96|P-value:6.39E-4 ID:SGTC_1619|Compound:st003326|FD-Score:3.57|P-value:5.14E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.95|P-value:8.52E-6 ID:SGTC_1966|Compound:st070145|FD-Score:-2.95|P-value:6.52E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.54|P-value:5.72E-5 ID:SGTC_2010|Compound:4012422|FD-Score:2.91|P-value:7.67E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-2.88|P-value:8.58E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.64|P-value:3.62E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-3.03|P-value:4.86E-4 ID:SGTC_2432|Compound:5630864|FD-Score:2.96|P-value:6.34E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.76|P-value:2.07E-5 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.69|P-value:2.86E-5 ID:SGTC_2647|Compound:atorvastatin|FD-Score:3.10|P-value:3.62E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:2.84|P-value:9.96E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:-3.67|P-value:3.18E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-5.25|P-value:5.49E-9 ID:SGTC_2852|Compound:9023854|FD-Score:3.45|P-value:8.59E-5 ID:SGTC_2879|Compound:9041815|FD-Score:2.93|P-value:7.10E-4

Top fitness defect scores for YDR308C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_937 3122-0005 5.67 46.7 µM 3.20E-10 1 6.96E-9 0.64
2 SGTC_1203 3992-3522 5.42 103.0 µM 1.77E-9 4 2.96E-8 0.12
3 SGTC_762 1165-0429 4.50 115.0 µM 4.86E-7 TSC3-RPN4 3 3.46E-6 0.90
4 SGTC_1745 st081495 3.95 74.0 µM 8.52E-6 RNA processing & uracil transport 35 3.93E-5 0.07
5 SGTC_2436 5633522 3.76 8.1 µM 2.07E-5 15 8.36E-5 0.25
6 SGTC_2454 5-benzylidenerhodanine 3.69 10.4 µM 2.86E-5 TRP & mitochondrial translation 6 1.10E-4 0.05
7 SGTC_2126 5251174 3.64 33.9 µM 3.62E-5 11 1.34E-4 0.04
8 SGTC_227 fluconazole 3.64 33.5 µM 3.70E-5 azole & statin 53 1.37E-4 0.19
9 SGTC_934 2950-0684 3.60 338.0 µM 4.35E-5 7 1.57E-4 0.09
10 SGTC_1619 st003326 3.57 88.7 µM 5.14E-5 60S ribosome export 6 1.81E-4 0.03
11 SGTC_2002 4023503 3.54 131.0 µM 5.72E-5 mitochondrial processes 13 1.98E-4 0.00
12 SGTC_882 0843-0131 3.54 351.0 µM 5.74E-5 15 1.99E-4 0.06
13 SGTC_2852 9023854 3.45 52.0 µM 8.59E-5 8 2.80E-4 0.00
14 SGTC_240 5hr heat shock (37°C) + Streptovitacin 3.41 300.0 µM 9.99E-5 heat shock/prefoldin 7 3.19E-4 0.20
15 SGTC_1167 k781-0610 3.21 148.0 µM 2.33E-4 RNA processing & uracil transport 16 6.56E-4 0.02
16 SGTC_1465 k213-0056 3.19 144.0 µM 2.55E-4 DNA intercalators 22 7.10E-4 0.09
17 SGTC_2647 atorvastatin 3.10 60.2 µM 3.62E-4 azole & statin 7 9.55E-4 0.00
18 SGTC_104 0074-0074 3.07 591.0 µM 4.15E-4 31 0.00107 0.04
19 SGTC_2432 5630864 2.96 12.2 µM 6.34E-4 21 0.00154 0.00
20 SGTC_1604 st002052 2.96 72.4 µM 6.39E-4 TSC3-RPN4 15 0.00155 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.77E-18 YER046W SPO73 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.144 4.57E-17 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.130 4.87E-14 YNL135C FPR1 hom Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function
0.128 1.22E-13 YFL056C AAD6 hom Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin
0.124 4.63E-13 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.118 5.84E-12 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.108 3.03E-10 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.108 4.20E-10 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.106 7.42E-10 YLR277C YSH1 het Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
0.102 2.93E-9 YDL165W CDC36 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.097 2.10E-8 YMR085W_p YMR085W_p hom Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
0.096 2.32E-8 YKL112W ABF1 het DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
0.095 3.21E-8 YDR228C PCF11 het mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
0.092 9.47E-8 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.091 1.34E-7 YNR025C_d YNR025C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase