YDR311W / TFB1

Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR311W / TFB1

Click on Significant Values for Screen Details ID:SGTC_75|Compound:0159-0059|FD-Score:4.23|P-value:3.55E-5 ID:SGTC_110|Compound:0850-0284|FD-Score:-3.75|P-value:1.90E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.53|P-value:1.02E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:5.39|P-value:2.05E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.22|P-value:3.82E-7 ID:SGTC_404|Compound:fumagillin|FD-Score:3.39|P-value:7.45E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.73|P-value:2.04E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.63|P-value:2.88E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.91|P-value:1.21E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.33|P-value:8.93E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:5.68|P-value:4.54E-8 ID:SGTC_1242|Compound:0402-0103|FD-Score:5.03|P-value:1.15E-6 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.32|P-value:9.12E-4 ID:SGTC_1719|Compound:st032227|FD-Score:4.81|P-value:3.16E-6 ID:SGTC_1745|Compound:st081495|FD-Score:4.35|P-value:2.17E-5 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.26|P-value:9.79E-4 ID:SGTC_1949|Compound:st076024|FD-Score:5.40|P-value:1.89E-7 ID:SGTC_1959|Compound:st077091|FD-Score:3.57|P-value:4.07E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.31|P-value:9.49E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-3.52|P-value:4.23E-4 ID:SGTC_2024|Compound:5144227|FD-Score:5.51|P-value:1.09E-7 ID:SGTC_2054|Compound:5233173|FD-Score:-4.02|P-value:6.80E-5 ID:SGTC_2068|Compound:5226020|FD-Score:5.00|P-value:1.28E-6 ID:SGTC_2089|Compound:5281248|FD-Score:6.55|P-value:3.56E-10 ID:SGTC_2172|Compound:5754775|FD-Score:3.45|P-value:6.01E-4 ID:SGTC_2180|Compound:6301957|FD-Score:-3.63|P-value:2.83E-4 ID:SGTC_2181|Compound:6316240|FD-Score:-4.49|P-value:1.05E-5 ID:SGTC_2182|Compound:6325192|FD-Score:-4.39|P-value:1.59E-5 ID:SGTC_2281|Compound:7943571|FD-Score:5.38|P-value:2.09E-7 ID:SGTC_2318|Compound:6029776|FD-Score:-5.35|P-value:2.00E-7 ID:SGTC_2323|Compound:6148992|FD-Score:-4.33|P-value:2.02E-5 ID:SGTC_2325|Compound:6158266|FD-Score:-4.70|P-value:4.25E-6 ID:SGTC_2327|Compound:6189656|FD-Score:-5.05|P-value:8.73E-7 ID:SGTC_2476|Compound:5806916|FD-Score:-3.57|P-value:3.58E-4 ID:SGTC_2478|Compound:5763493|FD-Score:5.15|P-value:6.49E-7 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-3.52|P-value:4.19E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.65|P-value:6.26E-6 ID:SGTC_2703|Compound:st077852|FD-Score:4.24|P-value:3.37E-5 ID:SGTC_2730|Compound:imipramine|FD-Score:3.63|P-value:3.30E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.31|P-value:9.66E-4 ID:SGTC_2870|Compound:9038528|FD-Score:-4.78|P-value:2.92E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-3.38|P-value:6.61E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-4.93|P-value:1.53E-6 ID:SGTC_3145|Compound:9085516|FD-Score:3.37|P-value:7.81E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.38|P-value:7.57E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:4.23|P-value:3.55E-5 ID:SGTC_110|Compound:0850-0284|FD-Score:-3.75|P-value:1.90E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.53|P-value:1.02E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:5.39|P-value:2.05E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.22|P-value:3.82E-7 ID:SGTC_404|Compound:fumagillin|FD-Score:3.39|P-value:7.45E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.73|P-value:2.04E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.63|P-value:2.88E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.91|P-value:1.21E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.33|P-value:8.93E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:5.68|P-value:4.54E-8 ID:SGTC_1242|Compound:0402-0103|FD-Score:5.03|P-value:1.15E-6 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.32|P-value:9.12E-4 ID:SGTC_1719|Compound:st032227|FD-Score:4.81|P-value:3.16E-6 ID:SGTC_1745|Compound:st081495|FD-Score:4.35|P-value:2.17E-5 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.26|P-value:9.79E-4 ID:SGTC_1949|Compound:st076024|FD-Score:5.40|P-value:1.89E-7 ID:SGTC_1959|Compound:st077091|FD-Score:3.57|P-value:4.07E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.31|P-value:9.49E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-3.52|P-value:4.23E-4 ID:SGTC_2024|Compound:5144227|FD-Score:5.51|P-value:1.09E-7 ID:SGTC_2054|Compound:5233173|FD-Score:-4.02|P-value:6.80E-5 ID:SGTC_2068|Compound:5226020|FD-Score:5.00|P-value:1.28E-6 ID:SGTC_2089|Compound:5281248|FD-Score:6.55|P-value:3.56E-10 ID:SGTC_2172|Compound:5754775|FD-Score:3.45|P-value:6.01E-4 ID:SGTC_2180|Compound:6301957|FD-Score:-3.63|P-value:2.83E-4 ID:SGTC_2181|Compound:6316240|FD-Score:-4.49|P-value:1.05E-5 ID:SGTC_2182|Compound:6325192|FD-Score:-4.39|P-value:1.59E-5 ID:SGTC_2281|Compound:7943571|FD-Score:5.38|P-value:2.09E-7 ID:SGTC_2318|Compound:6029776|FD-Score:-5.35|P-value:2.00E-7 ID:SGTC_2323|Compound:6148992|FD-Score:-4.33|P-value:2.02E-5 ID:SGTC_2325|Compound:6158266|FD-Score:-4.70|P-value:4.25E-6 ID:SGTC_2327|Compound:6189656|FD-Score:-5.05|P-value:8.73E-7 ID:SGTC_2476|Compound:5806916|FD-Score:-3.57|P-value:3.58E-4 ID:SGTC_2478|Compound:5763493|FD-Score:5.15|P-value:6.49E-7 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-3.52|P-value:4.19E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.65|P-value:6.26E-6 ID:SGTC_2703|Compound:st077852|FD-Score:4.24|P-value:3.37E-5 ID:SGTC_2730|Compound:imipramine|FD-Score:3.63|P-value:3.30E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.31|P-value:9.66E-4 ID:SGTC_2870|Compound:9038528|FD-Score:-4.78|P-value:2.92E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-3.38|P-value:6.61E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-4.93|P-value:1.53E-6 ID:SGTC_3145|Compound:9085516|FD-Score:3.37|P-value:7.81E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.38|P-value:7.57E-4

Top fitness defect scores for YDR311W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2089 5281248 6.55 129.7 µM 3.56E-10 superoxide 2 2.82E-11 1.17
2 SGTC_696 1560-0013 5.68 611.0 µM 4.54E-8 3 6.54E-9 0.31
3 SGTC_2024 5144227 5.51 134.0 µM 1.09E-7 2 1.74E-8 1.25
4 SGTC_1949 st076024 5.40 69.1 µM 1.89E-7 3 3.25E-8 0.77
5 SGTC_122 0236-0016 5.39 402.4 µM 2.05E-7 RNA processing & uracil transport 4 3.55E-8 0.02
6 SGTC_2281 7943571 5.38 200.0 µM 2.09E-7 2 3.64E-8 1.06
7 SGTC_2478 5763493 5.15 32.3 µM 6.49E-7 2 1.30E-7 0.45
8 SGTC_1242 0402-0103 5.03 60.1 µM 1.15E-6 2 2.47E-7 0.22
9 SGTC_2068 5226020 5.00 147.0 µM 1.28E-6 2 2.79E-7 0.46
10 SGTC_1719 st032227 4.81 49.2 µM 3.16E-6 5 7.68E-7 0.19
11 SGTC_2563 tomatine 4.65 8.5 µM 6.26E-6 10 1.66E-6 0.10
12 SGTC_118 0083-0097 4.53 135.3 µM 1.02E-5 translation 11 2.88E-6 0.06
13 SGTC_1745 st081495 4.35 74.0 µM 2.17E-5 RNA processing & uracil transport 25 6.68E-6 0.01
14 SGTC_2703 st077852 4.24 53.4 µM 3.37E-5 5 1.10E-5 0.12
15 SGTC_75 0159-0059 4.23 199.0 µM 3.55E-5 fatty acid desaturase (OLE1) 5 1.16E-5 0.01
16 SGTC_451 chloral hydrate 3.91 100.0 µM 1.21E-4 20 4.60E-5 0.18
17 SGTC_2730 imipramine 3.63 71.3 µM 3.30E-4 8 1.42E-4 0.06
18 SGTC_1959 st077091 3.57 55.8 µM 4.07E-4 RPP1 & pyrimidine depletion 28 1.79E-4 0.05
19 SGTC_2172 5754775 3.45 163.9 µM 6.01E-4 13 2.77E-4 0.01
20 SGTC_404 fumagillin 3.39 50.0 µM 7.45E-4 11 3.53E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.108 3.28E-10 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.106 7.88E-10 YBL070C_d YBL070C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.098 1.44E-8 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.095 3.71E-8 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.094 4.24E-8 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.093 7.37E-8 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
0.092 9.56E-8 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.091 1.43E-7 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.090 1.53E-7 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.089 2.09E-7 YAL060W BDH1 hom NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
0.086 5.31E-7 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.085 8.59E-7 YOL061W PRS5 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress
0.083 1.56E-6 YDR201W SPC19 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
0.083 1.67E-6 YBR228W SLX1 hom Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
0.082 2.03E-6 YKL036C_d YKL036C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W