YDR312W / SSF2

Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR312W / SSF2

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:4.27|P-value:8.09E-7 ID:SGTC_645|Compound:0845-0943|FD-Score:3.29|P-value:1.03E-4 ID:SGTC_721|Compound:1187-1332|FD-Score:-2.98|P-value:5.34E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.40|P-value:6.32E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.51|P-value:2.04E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.01|P-value:3.36E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.98|P-value:5.35E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.39|P-value:6.60E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:-3.44|P-value:7.81E-5 ID:SGTC_1295|Compound:1013-0063|FD-Score:4.12|P-value:1.77E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:-2.85|P-value:8.70E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:2.80|P-value:7.76E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.19|P-value:1.25E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.33|P-value:8.72E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:-3.01|P-value:4.65E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-8.91|P-value:2.44E-23 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.99|P-value:1.78E-8 ID:SGTC_1633|Compound:st005664|FD-Score:3.75|P-value:1.18E-5 ID:SGTC_1634|Compound:st006576|FD-Score:2.93|P-value:4.62E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-2.94|P-value:6.19E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-3.28|P-value:1.53E-4 ID:SGTC_2002|Compound:4023503|FD-Score:5.10|P-value:5.20E-9 ID:SGTC_2004|Compound:4023728|FD-Score:2.74|P-value:9.73E-4 ID:SGTC_2126|Compound:5251174|FD-Score:2.86|P-value:6.26E-4 ID:SGTC_2243|Compound:6634756|FD-Score:2.80|P-value:7.80E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.05|P-value:2.87E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.03|P-value:3.08E-4 ID:SGTC_2462|Compound:5162487|FD-Score:2.80|P-value:7.81E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.37|P-value:7.31E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.60|P-value:2.48E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.66|P-value:2.71E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:4.01|P-value:3.19E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-2.88|P-value:7.70E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.17|P-value:1.78E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.21|P-value:1.46E-4 ID:SGTC_3034|Compound:9090421|FD-Score:2.76|P-value:8.91E-4 ID:SGTC_3203|Compound:9113543|FD-Score:2.74|P-value:9.83E-4 ID:SGTC_3207|Compound:9114163|FD-Score:2.86|P-value:6.11E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:4.27|P-value:8.09E-7 ID:SGTC_645|Compound:0845-0943|FD-Score:3.29|P-value:1.03E-4 ID:SGTC_721|Compound:1187-1332|FD-Score:-2.98|P-value:5.34E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.40|P-value:6.32E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.51|P-value:2.04E-7 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.01|P-value:3.36E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.98|P-value:5.35E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.39|P-value:6.60E-5 ID:SGTC_1274|Compound:0865-0114|FD-Score:-3.44|P-value:7.81E-5 ID:SGTC_1295|Compound:1013-0063|FD-Score:4.12|P-value:1.77E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:-2.85|P-value:8.70E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:2.80|P-value:7.76E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.19|P-value:1.25E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.33|P-value:8.72E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:-3.01|P-value:4.65E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-8.91|P-value:2.44E-23 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.99|P-value:1.78E-8 ID:SGTC_1633|Compound:st005664|FD-Score:3.75|P-value:1.18E-5 ID:SGTC_1634|Compound:st006576|FD-Score:2.93|P-value:4.62E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-2.94|P-value:6.19E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-3.28|P-value:1.53E-4 ID:SGTC_2002|Compound:4023503|FD-Score:5.10|P-value:5.20E-9 ID:SGTC_2004|Compound:4023728|FD-Score:2.74|P-value:9.73E-4 ID:SGTC_2126|Compound:5251174|FD-Score:2.86|P-value:6.26E-4 ID:SGTC_2243|Compound:6634756|FD-Score:2.80|P-value:7.80E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.05|P-value:2.87E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.03|P-value:3.08E-4 ID:SGTC_2462|Compound:5162487|FD-Score:2.80|P-value:7.81E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.37|P-value:7.31E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.60|P-value:2.48E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.66|P-value:2.71E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:4.01|P-value:3.19E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:-2.88|P-value:7.70E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.17|P-value:1.78E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.21|P-value:1.46E-4 ID:SGTC_3034|Compound:9090421|FD-Score:2.76|P-value:8.91E-4 ID:SGTC_3203|Compound:9113543|FD-Score:2.74|P-value:9.83E-4 ID:SGTC_3207|Compound:9114163|FD-Score:2.86|P-value:6.11E-4

Top fitness defect scores for YDR312W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2002 4023503 5.10 131.0 µM 5.20E-9 mitochondrial processes 12 1.71E-7
2 SGTC_1034 k072-0165 4.51 12.7 µM 2.04E-7 Golgi 11 3.29E-6
3 SGTC_65 0798-0905 4.27 35.0 µM 8.09E-7 calcium & mitochondrial duress 76 9.97E-6
4 SGTC_1447 4239-0816 4.19 81.9 µM 1.25E-6 23 1.41E-5
5 SGTC_1295 1013-0063 4.12 9.8 µM 1.77E-6 6 1.88E-5
6 SGTC_2586 curcumin 4.01 80.0 µM 3.19E-6 iron homeostasis 48 3.01E-5
7 SGTC_1633 st005664 3.75 67.9 µM 1.18E-5 36 8.68E-5
8 SGTC_2571 chrysanthemic acid 3.60 100.0 µM 2.48E-5 34 1.58E-4
9 SGTC_725 1497-0963 3.40 19.0 µM 6.32E-5 copper-dependent oxidative stress 35 3.35E-4
10 SGTC_1230 0170-0365 3.39 3.8 µM 6.60E-5 endomembrane recycling 100 3.48E-4
11 SGTC_2564 betulinic acid 3.37 100.0 µM 7.31E-5 55 3.77E-4
12 SGTC_1450 4112-4260 3.33 37.6 µM 8.72E-5 calcium & mitochondrial duress 49 4.35E-4
13 SGTC_645 0845-0943 3.29 5.4 µM 1.03E-4 60S ribosome export 25 4.96E-4
14 SGTC_2967 9089538 3.21 49.9 µM 1.46E-4 65 6.61E-4
15 SGTC_2794 7785382 3.17 27.3 µM 1.78E-4 75 7.75E-4
16 SGTC_2297 7971671 3.05 152.3 µM 2.87E-4 RNA processing & uracil transport 60 0.00114
17 SGTC_2329 6138490 3.03 200.0 µM 3.08E-4 85 0.00121
18 SGTC_1035 k081-0041 3.01 55.2 µM 3.36E-4 Golgi 71 0.00130
19 SGTC_1634 st006576 2.93 41.0 µM 4.62E-4 TSC3-RPN4 62 0.00167
20 SGTC_3207 9114163 2.86 49.5 µM 6.11E-4 97 0.00210

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.398 1.94E-127 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.375 3.14E-112 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.360 4.92E-103 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.354 9.83E-99 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.341 2.17E-91 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.323 7.16E-82 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.321 3.37E-81 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.316 1.57E-78 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.316 1.65E-78 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.315 7.32E-78 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.306 1.33E-73 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.299 6.62E-70 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.297 6.27E-69 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.280 2.69E-61 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.280 2.90E-61 YLR213C CRR1 hom Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation