YDR318W / MCM21

Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR318W / MCM21

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:4.83|P-value:7.36E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:3.78|P-value:3.57E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.96|P-value:1.93E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:6.86|P-value:3.86E-10 ID:SGTC_229|Compound:benomyl|FD-Score:4.31|P-value:5.71E-5 ID:SGTC_235|Compound:0330-0087|FD-Score:-3.45|P-value:9.84E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:-3.78|P-value:3.47E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:6.59|P-value:1.72E-9 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:7.47|P-value:1.09E-11 ID:SGTC_255|Compound:tunicamycin|FD-Score:-3.92|P-value:2.14E-4 ID:SGTC_379|Compound:3970-0972|FD-Score:3.61|P-value:6.19E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.92|P-value:2.20E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.45|P-value:3.70E-9 ID:SGTC_528|Compound:3492-0019|FD-Score:4.22|P-value:7.87E-5 ID:SGTC_534|Compound:k292-0788|FD-Score:3.48|P-value:9.16E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:4.82|P-value:7.69E-6 ID:SGTC_863|Compound:0466-0191|FD-Score:3.81|P-value:3.23E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.66|P-value:5.13E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:8.75|P-value:2.23E-15 ID:SGTC_996|Compound:3970-0799|FD-Score:6.28|P-value:8.85E-9 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.77|P-value:3.63E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.52|P-value:7.79E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-4.17|P-value:9.02E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:4.86|P-value:6.70E-6 ID:SGTC_1368|Compound:1852-0141|FD-Score:-5.54|P-value:3.33E-7 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.56|P-value:2.16E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.58|P-value:2.02E-5 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.95|P-value:1.97E-4 ID:SGTC_1412|Compound:3909-8563|FD-Score:4.91|P-value:5.36E-6 ID:SGTC_1529|Compound:apigenin|FD-Score:3.62|P-value:5.97E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.84|P-value:2.86E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:5.63|P-value:2.21E-7 ID:SGTC_1900|Compound:st060200|FD-Score:-3.98|P-value:1.79E-4 ID:SGTC_2040|Compound:5100729|FD-Score:13.30|P-value:3.85E-33 ID:SGTC_2165|Compound:5727197|FD-Score:-4.42|P-value:3.62E-5 ID:SGTC_2238|Compound:6671620|FD-Score:-3.83|P-value:2.91E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.55|P-value:7.24E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.55|P-value:7.19E-4 ID:SGTC_2357|Compound:9067248|FD-Score:4.65|P-value:1.53E-5 ID:SGTC_2437|Compound:5741531|FD-Score:5.04|P-value:3.13E-6 ID:SGTC_2485|Compound:5633444|FD-Score:3.95|P-value:1.99E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.81|P-value:3.23E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.12|P-value:2.03E-8 ID:SGTC_2591|Compound:skatole|FD-Score:3.46|P-value:9.72E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.54|P-value:2.34E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:3.55|P-value:7.32E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.12|P-value:1.11E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.46|P-value:9.73E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.98|P-value:1.82E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.90|P-value:2.34E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.54|P-value:7.48E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-4.17|P-value:9.12E-5 ID:SGTC_2991|Compound:9074669|FD-Score:5.09|P-value:2.50E-6 ID:SGTC_2999|Compound:9069765|FD-Score:6.06|P-value:2.86E-8 ID:SGTC_3215|Compound:9129942|FD-Score:4.12|P-value:1.10E-4 ID:SGTC_3217|Compound:9129673|FD-Score:4.26|P-value:6.81E-5 ID:SGTC_3311|Compound:9131146|FD-Score:6.09|P-value:2.46E-8 ID:SGTC_3350|Compound:9150499|FD-Score:4.25|P-value:6.95E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:4.83|P-value:7.36E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:3.78|P-value:3.57E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.96|P-value:1.93E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:6.86|P-value:3.86E-10 ID:SGTC_229|Compound:benomyl|FD-Score:4.31|P-value:5.71E-5 ID:SGTC_235|Compound:0330-0087|FD-Score:-3.45|P-value:9.84E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:-3.78|P-value:3.47E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:6.59|P-value:1.72E-9 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:7.47|P-value:1.09E-11 ID:SGTC_255|Compound:tunicamycin|FD-Score:-3.92|P-value:2.14E-4 ID:SGTC_379|Compound:3970-0972|FD-Score:3.61|P-value:6.19E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.92|P-value:2.20E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.45|P-value:3.70E-9 ID:SGTC_528|Compound:3492-0019|FD-Score:4.22|P-value:7.87E-5 ID:SGTC_534|Compound:k292-0788|FD-Score:3.48|P-value:9.16E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:4.82|P-value:7.69E-6 ID:SGTC_863|Compound:0466-0191|FD-Score:3.81|P-value:3.23E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.66|P-value:5.13E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:8.75|P-value:2.23E-15 ID:SGTC_996|Compound:3970-0799|FD-Score:6.28|P-value:8.85E-9 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.77|P-value:3.63E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.52|P-value:7.79E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-4.17|P-value:9.02E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:4.86|P-value:6.70E-6 ID:SGTC_1368|Compound:1852-0141|FD-Score:-5.54|P-value:3.33E-7 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.56|P-value:2.16E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.58|P-value:2.02E-5 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.95|P-value:1.97E-4 ID:SGTC_1412|Compound:3909-8563|FD-Score:4.91|P-value:5.36E-6 ID:SGTC_1529|Compound:apigenin|FD-Score:3.62|P-value:5.97E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.84|P-value:2.86E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:5.63|P-value:2.21E-7 ID:SGTC_1900|Compound:st060200|FD-Score:-3.98|P-value:1.79E-4 ID:SGTC_2040|Compound:5100729|FD-Score:13.30|P-value:3.85E-33 ID:SGTC_2165|Compound:5727197|FD-Score:-4.42|P-value:3.62E-5 ID:SGTC_2238|Compound:6671620|FD-Score:-3.83|P-value:2.91E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.55|P-value:7.24E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.55|P-value:7.19E-4 ID:SGTC_2357|Compound:9067248|FD-Score:4.65|P-value:1.53E-5 ID:SGTC_2437|Compound:5741531|FD-Score:5.04|P-value:3.13E-6 ID:SGTC_2485|Compound:5633444|FD-Score:3.95|P-value:1.99E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.81|P-value:3.23E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.12|P-value:2.03E-8 ID:SGTC_2591|Compound:skatole|FD-Score:3.46|P-value:9.72E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.54|P-value:2.34E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:3.55|P-value:7.32E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.12|P-value:1.11E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.46|P-value:9.73E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.98|P-value:1.82E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.90|P-value:2.34E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.54|P-value:7.48E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-4.17|P-value:9.12E-5 ID:SGTC_2991|Compound:9074669|FD-Score:5.09|P-value:2.50E-6 ID:SGTC_2999|Compound:9069765|FD-Score:6.06|P-value:2.86E-8 ID:SGTC_3215|Compound:9129942|FD-Score:4.12|P-value:1.10E-4 ID:SGTC_3217|Compound:9129673|FD-Score:4.26|P-value:6.81E-5 ID:SGTC_3311|Compound:9131146|FD-Score:6.09|P-value:2.46E-8 ID:SGTC_3350|Compound:9150499|FD-Score:4.25|P-value:6.95E-5

Top fitness defect scores for YDR318W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2040 5100729 13.30 123.0 µM 3.85E-33 1 9.82E-41
2 SGTC_916 4466-0038 8.75 42.0 nM 2.23E-15 cell wall 8 1.10E-18
3 SGTC_241 5hr heat shock (37°C) + cantharidin 7.47 155.4 µM 1.09E-11 cell wall signaling 20 4.09E-14
4 SGTC_212 3807-4682 6.86 38.8 µM 3.86E-10 tubulin folding & SWR complex 18 3.41E-12
5 SGTC_239 5hr heat shock (37°C) + mitomycin C 6.59 819.4 µM 1.72E-9 heat shock/prefoldin 9 2.17E-11
6 SGTC_514 4466-0038 6.45 2.6 nM 3.70E-9 cell wall 63 5.58E-11
7 SGTC_996 3970-0799 6.28 1.8 µM 8.85E-9 31 1.64E-10
8 SGTC_2584 androsterone 6.12 48.7 µM 2.03E-8 cell wall 56 4.58E-10
9 SGTC_3311 9131146 6.09 2.9 µM 2.46E-8 cell wall 59 5.81E-10
10 SGTC_2999 9069765 6.06 71.4 µM 2.86E-8 amide catabolism 4 7.00E-10
11 SGTC_1880 MG-132 5.63 525.0 µM 2.21E-7 6 8.77E-9
12 SGTC_2991 9074669 5.09 71.4 µM 2.50E-6 azole & statin 30 1.76E-7
13 SGTC_2437 5741531 5.04 144.2 µM 3.13E-6 20 2.32E-7
14 SGTC_1412 3909-8563 4.91 178.0 µM 5.36E-6 1 4.50E-7
15 SGTC_1356 1502-0781 4.86 85.8 µM 6.70E-6 fatty acid desaturase (OLE1) 7 5.94E-7
16 SGTC_43 0570-0105 4.83 72.5 µM 7.36E-6 ERG2 56 6.67E-7
17 SGTC_563 tetrahydrosecaminediol 4.82 614.0 µM 7.69E-6 amide catabolism 7 7.04E-7
18 SGTC_2357 9067248 4.65 200.0 µM 1.53E-5 8 1.64E-6
19 SGTC_1375 2910-0797 4.58 186.0 µM 2.02E-5 17 2.32E-6
20 SGTC_1369 1866-0067 4.56 208.0 µM 2.16E-5 RPP1 & pyrimidine depletion 24 2.52E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.294 5.49E-68 YPL017C IRC15 hom Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.243 1.89E-46 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.233 1.18E-42 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.220 3.74E-38 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.220 5.60E-38 YPR120C CLB5 hom B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase
0.217 4.72E-37 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.216 1.33E-36 YLR212C TUB4 het Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.203 1.37E-32 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.200 1.60E-31 YDR254W CHL4 hom Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
0.196 1.93E-30 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.195 5.01E-30 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.184 5.61E-27 YNL126W SPC98 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.184 6.45E-27 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.184 7.96E-27 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.183 1.09E-26 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles