YDR319C_p / YFT2

Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR319C / YFT2

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:-3.60|P-value:1.81E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.40|P-value:3.79E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.51|P-value:2.21E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:5.22|P-value:9.22E-8 ID:SGTC_131|Compound:0141-0277|FD-Score:3.29|P-value:4.97E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.99|P-value:3.25E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.88|P-value:5.40E-7 ID:SGTC_220|Compound:paclitaxel|FD-Score:3.81|P-value:6.93E-5 ID:SGTC_316|Compound:k060-0003|FD-Score:3.21|P-value:6.59E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.31|P-value:4.63E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.96|P-value:3.71E-5 ID:SGTC_891|Compound:0929-0073|FD-Score:3.29|P-value:5.05E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.48|P-value:2.80E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:6.24|P-value:2.36E-10 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.32|P-value:4.45E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.54|P-value:1.97E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.49|P-value:2.40E-4 ID:SGTC_1636|Compound:st007707|FD-Score:4.43|P-value:4.74E-6 ID:SGTC_1749|Compound:st037932|FD-Score:3.23|P-value:6.16E-4 ID:SGTC_1771|Compound:st048882|FD-Score:3.57|P-value:1.76E-4 ID:SGTC_1833|Compound:st055984|FD-Score:-3.13|P-value:9.60E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:-3.65|P-value:1.46E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.46|P-value:2.64E-4 ID:SGTC_1948|Compound:st076329|FD-Score:3.14|P-value:8.36E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.17|P-value:7.58E-4 ID:SGTC_1968|Compound:st069590|FD-Score:4.15|P-value:1.70E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-4.74|P-value:1.29E-6 ID:SGTC_2273|Compound:7815444|FD-Score:-3.31|P-value:5.12E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-3.30|P-value:5.30E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.24|P-value:6.03E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.07|P-value:2.37E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.44|P-value:2.88E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.28|P-value:5.13E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.28|P-value:5.17E-4 ID:SGTC_2365|Compound:9042475|FD-Score:3.85|P-value:5.80E-5 ID:SGTC_2389|Compound:9091542|FD-Score:4.65|P-value:1.67E-6 ID:SGTC_2390|Compound:9097792|FD-Score:4.36|P-value:6.52E-6 ID:SGTC_2423|Compound:st077119|FD-Score:-3.46|P-value:2.97E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.66|P-value:1.25E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:-3.60|P-value:1.77E-4 ID:SGTC_2696|Compound:st077691|FD-Score:-3.47|P-value:2.87E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-4.76|P-value:1.13E-6 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-4.44|P-value:5.20E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:5.36|P-value:4.28E-8 ID:SGTC_2772|Compound:vinpocetine|FD-Score:5.75|P-value:4.58E-9 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.85|P-value:5.81E-5 ID:SGTC_2919|Compound:7978188|FD-Score:3.41|P-value:3.21E-4 ID:SGTC_3125|Compound:9127534|FD-Score:4.62|P-value:1.94E-6 ID:SGTC_3152|Compound:9099253|FD-Score:4.62|P-value:1.95E-6 ID:SGTC_3158|Compound:9100007|FD-Score:-3.51|P-value:2.50E-4 ID:SGTC_3178|Compound:9107506|FD-Score:4.90|P-value:4.90E-7 ID:SGTC_3292|Compound:9120825|FD-Score:-3.83|P-value:7.11E-5 ID:SGTC_3325|Compound:9138760|FD-Score:-7.80|P-value:4.62E-15 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.60|P-value:1.81E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.40|P-value:3.79E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.51|P-value:2.21E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:5.22|P-value:9.22E-8 ID:SGTC_131|Compound:0141-0277|FD-Score:3.29|P-value:4.97E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.99|P-value:3.25E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.88|P-value:5.40E-7 ID:SGTC_220|Compound:paclitaxel|FD-Score:3.81|P-value:6.93E-5 ID:SGTC_316|Compound:k060-0003|FD-Score:3.21|P-value:6.59E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.31|P-value:4.63E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.96|P-value:3.71E-5 ID:SGTC_891|Compound:0929-0073|FD-Score:3.29|P-value:5.05E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.48|P-value:2.80E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:6.24|P-value:2.36E-10 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.32|P-value:4.45E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.54|P-value:1.97E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.49|P-value:2.40E-4 ID:SGTC_1636|Compound:st007707|FD-Score:4.43|P-value:4.74E-6 ID:SGTC_1749|Compound:st037932|FD-Score:3.23|P-value:6.16E-4 ID:SGTC_1771|Compound:st048882|FD-Score:3.57|P-value:1.76E-4 ID:SGTC_1833|Compound:st055984|FD-Score:-3.13|P-value:9.60E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:-3.65|P-value:1.46E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.46|P-value:2.64E-4 ID:SGTC_1948|Compound:st076329|FD-Score:3.14|P-value:8.36E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.17|P-value:7.58E-4 ID:SGTC_1968|Compound:st069590|FD-Score:4.15|P-value:1.70E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-4.74|P-value:1.29E-6 ID:SGTC_2273|Compound:7815444|FD-Score:-3.31|P-value:5.12E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-3.30|P-value:5.30E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.24|P-value:6.03E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.07|P-value:2.37E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.44|P-value:2.88E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.28|P-value:5.13E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.28|P-value:5.17E-4 ID:SGTC_2365|Compound:9042475|FD-Score:3.85|P-value:5.80E-5 ID:SGTC_2389|Compound:9091542|FD-Score:4.65|P-value:1.67E-6 ID:SGTC_2390|Compound:9097792|FD-Score:4.36|P-value:6.52E-6 ID:SGTC_2423|Compound:st077119|FD-Score:-3.46|P-value:2.97E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.66|P-value:1.25E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:-3.60|P-value:1.77E-4 ID:SGTC_2696|Compound:st077691|FD-Score:-3.47|P-value:2.87E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-4.76|P-value:1.13E-6 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-4.44|P-value:5.20E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:5.36|P-value:4.28E-8 ID:SGTC_2772|Compound:vinpocetine|FD-Score:5.75|P-value:4.58E-9 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.85|P-value:5.81E-5 ID:SGTC_2919|Compound:7978188|FD-Score:3.41|P-value:3.21E-4 ID:SGTC_3125|Compound:9127534|FD-Score:4.62|P-value:1.94E-6 ID:SGTC_3152|Compound:9099253|FD-Score:4.62|P-value:1.95E-6 ID:SGTC_3158|Compound:9100007|FD-Score:-3.51|P-value:2.50E-4 ID:SGTC_3178|Compound:9107506|FD-Score:4.90|P-value:4.90E-7 ID:SGTC_3292|Compound:9120825|FD-Score:-3.83|P-value:7.11E-5 ID:SGTC_3325|Compound:9138760|FD-Score:-7.80|P-value:4.62E-15

Top fitness defect scores for YDR319C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_904 1141-0088 6.24 396.0 µM 2.36E-10 2 2.22E-10
2 SGTC_2772 vinpocetine 5.75 57.1 µM 4.58E-9 6 4.38E-9
3 SGTC_2756 carbadox 5.36 75.1 µM 4.28E-8 26 4.14E-8
4 SGTC_102 gallobenzophenone 5.22 83.6 µM 9.22E-8 redox potentiating 19 8.95E-8
5 SGTC_3178 9107506 4.90 49.5 µM 4.90E-7 3 4.80E-7
6 SGTC_217 ketoconazole 4.88 989.0 nM 5.40E-7 ergosterol depletion effects on membrane 14 5.30E-7
7 SGTC_2389 9091542 4.65 195.5 µM 1.67E-6 iron homeostasis 23 1.64E-6
8 SGTC_3125 9127534 4.62 49.5 µM 1.94E-6 5 1.91E-6
9 SGTC_3152 9099253 4.62 49.5 µM 1.95E-6 NEO1-PIK1 97 1.93E-6
10 SGTC_1636 st007707 4.43 68.9 µM 4.74E-6 40 4.69E-6
11 SGTC_2390 9097792 4.36 176.6 µM 6.52E-6 7 6.46E-6
12 SGTC_1968 st069590 4.15 43.6 µM 1.70E-5 13 1.69E-5
13 SGTC_2296 7971656 4.07 126.5 µM 2.37E-5 40 2.37E-5
14 SGTC_143 4032-1194 3.99 15.7 µM 3.25E-5 65 3.25E-5
15 SGTC_890 0929-0006 3.96 47.2 µM 3.71E-5 18 3.71E-5
16 SGTC_2365 9042475 3.85 200.0 µM 5.80E-5 38 5.80E-5
17 SGTC_2776 terbinafine 3.85 19.9 µM 5.81E-5 cell wall signaling 56 5.81E-5
18 SGTC_220 paclitaxel 3.81 373.4 µM 6.93E-5 11 6.94E-5
19 SGTC_2432 5630864 3.66 12.2 µM 1.25E-4 32 1.26E-4
20 SGTC_1771 st048882 3.57 4.2 µM 1.76E-4 RPP1 & pyrimidine depletion 32 1.77E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.50E-10 YDR524C AGE1 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
0.109 2.72E-10 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.108 3.44E-10 YNL311C SKP2 hom F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
0.107 4.33E-10 YDL240W LRG1 hom Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis
0.097 1.79E-8 YGR176W_d YGR176W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.094 5.31E-8 YPR096C YPR096C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments
0.093 6.48E-8 YGR117C_p YGR117C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.088 2.95E-7 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.087 4.17E-7 YBR151W APD1 hom Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
0.086 6.25E-7 YDR228C PCF11 het mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
0.085 8.64E-7 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.080 3.30E-6 YOR068C VAM10 hom Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
0.078 6.36E-6 YDR034C LYS14 hom Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
0.076 9.43E-6 YDL141W BPL1 het Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
0.076 9.98E-6 YHR185C PFS1 hom Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation