YDR327W_d

Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR327W / YDR327W

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:3.18|P-value:3.71E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:3.52|P-value:9.59E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.02|P-value:6.67E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.69|P-value:7.66E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.18|P-value:5.60E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.33|P-value:3.24E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.94|P-value:2.60E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.42|P-value:1.47E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.10|P-value:7.53E-4 ID:SGTC_1688|Compound:st021091|FD-Score:3.98|P-value:1.31E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.14|P-value:6.47E-4 ID:SGTC_1814|Compound:st052110|FD-Score:4.55|P-value:8.02E-7 ID:SGTC_1822|Compound:st052468|FD-Score:-3.43|P-value:2.13E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.34|P-value:2.33E-6 ID:SGTC_1959|Compound:st077091|FD-Score:3.68|P-value:4.90E-5 ID:SGTC_1982|Compound:st072467|FD-Score:3.44|P-value:1.32E-4 ID:SGTC_2329|Compound:6138490|FD-Score:2.96|P-value:8.36E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.12|P-value:4.68E-4 ID:SGTC_2414|Compound:st077232|FD-Score:-3.68|P-value:7.73E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-5.64|P-value:2.82E-9 ID:SGTC_2581|Compound:avocatin a|FD-Score:4.15|P-value:5.67E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.79|P-value:4.94E-5 ID:SGTC_2789|Compound:5281112|FD-Score:-3.07|P-value:8.47E-4 ID:SGTC_2818|Compound:7996756|FD-Score:-3.02|P-value:9.81E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.32|P-value:3.28E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.18|P-value:3.71E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:3.52|P-value:9.59E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.02|P-value:6.67E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.69|P-value:7.66E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.18|P-value:5.60E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.33|P-value:3.24E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.94|P-value:2.60E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.42|P-value:1.47E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.10|P-value:7.53E-4 ID:SGTC_1688|Compound:st021091|FD-Score:3.98|P-value:1.31E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.14|P-value:6.47E-4 ID:SGTC_1814|Compound:st052110|FD-Score:4.55|P-value:8.02E-7 ID:SGTC_1822|Compound:st052468|FD-Score:-3.43|P-value:2.13E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.34|P-value:2.33E-6 ID:SGTC_1959|Compound:st077091|FD-Score:3.68|P-value:4.90E-5 ID:SGTC_1982|Compound:st072467|FD-Score:3.44|P-value:1.32E-4 ID:SGTC_2329|Compound:6138490|FD-Score:2.96|P-value:8.36E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.12|P-value:4.68E-4 ID:SGTC_2414|Compound:st077232|FD-Score:-3.68|P-value:7.73E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-5.64|P-value:2.82E-9 ID:SGTC_2581|Compound:avocatin a|FD-Score:4.15|P-value:5.67E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.79|P-value:4.94E-5 ID:SGTC_2789|Compound:5281112|FD-Score:-3.07|P-value:8.47E-4 ID:SGTC_2818|Compound:7996756|FD-Score:-3.02|P-value:9.81E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.32|P-value:3.28E-4

Top fitness defect scores for YDR327W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1814 st052110 4.55 31.5 µM 8.02E-7 RNA processing & uracil transport 5 2.74E-6 0.05
2 SGTC_1948 st076329 4.34 66.6 µM 2.33E-6 2 7.26E-6 0.35
3 SGTC_2581 avocatin a 4.15 64.0 µM 5.67E-6 sphingolipid biosynthesis & PDR1 5 1.63E-5 0.15
4 SGTC_1688 st021091 3.98 57.6 µM 1.31E-5 2 3.50E-5 0.24
5 SGTC_1959 st077091 3.68 55.8 µM 4.90E-5 RPP1 & pyrimidine depletion 26 1.17E-4 0.10
6 SGTC_295 k015-0027 3.52 31.0 µM 9.59E-5 PDR1 3 2.15E-4 0.23
7 SGTC_1982 st072467 3.44 71.9 µM 1.32E-4 11 2.88E-4 0.08
8 SGTC_1136 pergolide 3.42 1.1 µM 1.47E-4 30 3.17E-4 0.11
9 SGTC_20 0481-0013 3.18 4.9 µM 3.71E-4 15 7.38E-4 0.01
10 SGTC_2411 st077133 3.12 39.9 µM 4.68E-4 RNA pol III & RNase P/MRP 35 9.11E-4 0.05
11 SGTC_370 0180-0345 3.02 38.7 µM 6.67E-4 DNA intercalators 18 0.00126 0.14
12 SGTC_2329 6138490 2.96 200.0 µM 8.36E-4 27 0.00155 0.00
13 SGTC_1834 st055981 2.88 52.8 µM 0.00109 RPP1 & pyrimidine depletion 27 0.00197 0.04
14 SGTC_879 0831-1030 2.86 162.0 µM 0.00116 40 0.00209 0.07
15 SGTC_1023 4482-3888 2.85 16.0 µM 0.00122 TSC3-RPN4 25 0.00218 0.09
16 SGTC_3253 9135987 2.84 49.5 µM 0.00129 60S ribosome export 13 0.00229 0.03
17 SGTC_3127 9126946 2.83 49.5 µM 0.00130 ERG2 23 0.00231 0.04
18 SGTC_2008 4012253 2.80 172.0 µM 0.00146 26 0.00257 0.01
19 SGTC_345 1362-0200 2.76 395.0 µM 0.00168 16 0.00291 0.00
20 SGTC_1658 st012944 2.75 20.0 µM 0.00173 iron homeostasis 29 0.00300 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.57E-18 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.147 9.76E-18 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.117 8.56E-12 YFL009W CDC4 het F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.111 9.87E-11 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.106 6.23E-10 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.105 9.53E-10 YLR286C CTS1 hom Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
0.098 1.49E-8 YOR087W YVC1 hom Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
0.097 1.63E-8 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
0.096 2.64E-8 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.094 4.88E-8 YNL157W IGO1 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p
0.094 5.52E-8 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.093 7.71E-8 YBR236C ABD1 het Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
0.092 8.34E-8 YKR083C DAD2 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.092 8.31E-8 YPL011C TAF3 het TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.090 2.03E-7 YIL119C RPI1 hom Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF