YDR328C / SKP1

Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR328C / SKP1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:2.84|P-value:8.40E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:2.94|P-value:5.79E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.64|P-value:2.73E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.28|P-value:1.43E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.46|P-value:5.46E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.23|P-value:1.54E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.46|P-value:5.59E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:4.08|P-value:3.06E-6 ID:SGTC_276|Compound:colchicine|FD-Score:3.69|P-value:2.16E-5 ID:SGTC_296|Compound:1222-0039|FD-Score:3.45|P-value:6.66E-5 ID:SGTC_297|Compound:0986-0032|FD-Score:3.92|P-value:7.18E-6 ID:SGTC_307|Compound:1501-1223|FD-Score:4.60|P-value:1.77E-7 ID:SGTC_324|Compound:1315-0088|FD-Score:-2.83|P-value:7.78E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.98|P-value:5.25E-6 ID:SGTC_353|Compound:1988-1175|FD-Score:3.06|P-value:3.52E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.55|P-value:4.18E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:4.42|P-value:4.78E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.42|P-value:6.75E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.45|P-value:5.79E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.51|P-value:5.00E-5 ID:SGTC_470|Compound:aa-861|FD-Score:2.97|P-value:5.11E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.03|P-value:3.97E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:5.63|P-value:2.16E-10 ID:SGTC_569|Compound:4130-2812|FD-Score:3.51|P-value:5.20E-5 ID:SGTC_571|Compound:k831-0106|FD-Score:3.05|P-value:3.73E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:2.95|P-value:5.60E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.27|P-value:1.50E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.23|P-value:1.76E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.09|P-value:3.14E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.28|P-value:1.43E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.69|P-value:2.20E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:2.95|P-value:5.53E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.06|P-value:3.52E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:4.25|P-value:1.24E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:-2.86|P-value:7.02E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.83|P-value:8.81E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:2.93|P-value:5.99E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.80|P-value:1.27E-5 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.46|P-value:6.35E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:2.85|P-value:8.22E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.62|P-value:3.01E-5 ID:SGTC_1202|Compound:1988-0068|FD-Score:2.80|P-value:9.91E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.96|P-value:4.70E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.28|P-value:1.21E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:-2.91|P-value:5.64E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.32|P-value:8.64E-7 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.85|P-value:7.19E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.70|P-value:1.75E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-7.23|P-value:3.83E-16 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.02|P-value:3.60E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.29|P-value:1.01E-6 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.23|P-value:1.74E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.64|P-value:2.42E-5 ID:SGTC_1959|Compound:st077091|FD-Score:6.53|P-value:2.11E-13 ID:SGTC_2003|Compound:4023581|FD-Score:4.11|P-value:2.71E-6 ID:SGTC_2148|Compound:5546263|FD-Score:3.12|P-value:2.80E-4 ID:SGTC_2288|Compound:7946289|FD-Score:5.08|P-value:8.83E-9 ID:SGTC_2299|Compound:7976368|FD-Score:-3.48|P-value:4.97E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-2.86|P-value:6.98E-4 ID:SGTC_2450|Compound:5739737|FD-Score:5.54|P-value:4.18E-10 ID:SGTC_2563|Compound:tomatine|FD-Score:8.27|P-value:2.08E-20 ID:SGTC_2605|Compound:digitonin|FD-Score:2.87|P-value:7.42E-4 ID:SGTC_2714|Compound:st079313|FD-Score:-3.22|P-value:1.60E-4 ID:SGTC_2794|Compound:7785382|FD-Score:5.75|P-value:9.37E-11 ID:SGTC_2844|Compound:9010103|FD-Score:-2.92|P-value:5.47E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.25|P-value:1.63E-4 ID:SGTC_3095|Compound:9117207|FD-Score:2.83|P-value:8.72E-4 ID:SGTC_3142|Compound:9086498|FD-Score:2.85|P-value:7.98E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.61|P-value:2.51E-10 ID:SGTC_3224|Compound:9130643|FD-Score:3.39|P-value:8.88E-5 ID:SGTC_3290|Compound:9121031|FD-Score:3.41|P-value:8.13E-5 ID:SGTC_3307|Compound:9126885|FD-Score:3.32|P-value:1.18E-4 ID:SGTC_3329|Compound:9141233|FD-Score:2.84|P-value:8.28E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:2.84|P-value:8.40E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:2.94|P-value:5.79E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.64|P-value:2.73E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.28|P-value:1.43E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.46|P-value:5.46E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.23|P-value:1.54E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.46|P-value:5.59E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:4.08|P-value:3.06E-6 ID:SGTC_276|Compound:colchicine|FD-Score:3.69|P-value:2.16E-5 ID:SGTC_296|Compound:1222-0039|FD-Score:3.45|P-value:6.66E-5 ID:SGTC_297|Compound:0986-0032|FD-Score:3.92|P-value:7.18E-6 ID:SGTC_307|Compound:1501-1223|FD-Score:4.60|P-value:1.77E-7 ID:SGTC_324|Compound:1315-0088|FD-Score:-2.83|P-value:7.78E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.98|P-value:5.25E-6 ID:SGTC_353|Compound:1988-1175|FD-Score:3.06|P-value:3.52E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.55|P-value:4.18E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:4.42|P-value:4.78E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.42|P-value:6.75E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.45|P-value:5.79E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.51|P-value:5.00E-5 ID:SGTC_470|Compound:aa-861|FD-Score:2.97|P-value:5.11E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.03|P-value:3.97E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:5.63|P-value:2.16E-10 ID:SGTC_569|Compound:4130-2812|FD-Score:3.51|P-value:5.20E-5 ID:SGTC_571|Compound:k831-0106|FD-Score:3.05|P-value:3.73E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:2.95|P-value:5.60E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.27|P-value:1.50E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.23|P-value:1.76E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.09|P-value:3.14E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.28|P-value:1.43E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.69|P-value:2.20E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:2.95|P-value:5.53E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.06|P-value:3.52E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:4.25|P-value:1.24E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:-2.86|P-value:7.02E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.83|P-value:8.81E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:2.93|P-value:5.99E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.80|P-value:1.27E-5 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.46|P-value:6.35E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:2.85|P-value:8.22E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.62|P-value:3.01E-5 ID:SGTC_1202|Compound:1988-0068|FD-Score:2.80|P-value:9.91E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.96|P-value:4.70E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.28|P-value:1.21E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:-2.91|P-value:5.64E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.32|P-value:8.64E-7 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.85|P-value:7.19E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.70|P-value:1.75E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-7.23|P-value:3.83E-16 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.02|P-value:3.60E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.29|P-value:1.01E-6 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.23|P-value:1.74E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.64|P-value:2.42E-5 ID:SGTC_1959|Compound:st077091|FD-Score:6.53|P-value:2.11E-13 ID:SGTC_2003|Compound:4023581|FD-Score:4.11|P-value:2.71E-6 ID:SGTC_2148|Compound:5546263|FD-Score:3.12|P-value:2.80E-4 ID:SGTC_2288|Compound:7946289|FD-Score:5.08|P-value:8.83E-9 ID:SGTC_2299|Compound:7976368|FD-Score:-3.48|P-value:4.97E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-2.86|P-value:6.98E-4 ID:SGTC_2450|Compound:5739737|FD-Score:5.54|P-value:4.18E-10 ID:SGTC_2563|Compound:tomatine|FD-Score:8.27|P-value:2.08E-20 ID:SGTC_2605|Compound:digitonin|FD-Score:2.87|P-value:7.42E-4 ID:SGTC_2714|Compound:st079313|FD-Score:-3.22|P-value:1.60E-4 ID:SGTC_2794|Compound:7785382|FD-Score:5.75|P-value:9.37E-11 ID:SGTC_2844|Compound:9010103|FD-Score:-2.92|P-value:5.47E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.25|P-value:1.63E-4 ID:SGTC_3095|Compound:9117207|FD-Score:2.83|P-value:8.72E-4 ID:SGTC_3142|Compound:9086498|FD-Score:2.85|P-value:7.98E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.61|P-value:2.51E-10 ID:SGTC_3224|Compound:9130643|FD-Score:3.39|P-value:8.88E-5 ID:SGTC_3290|Compound:9121031|FD-Score:3.41|P-value:8.13E-5 ID:SGTC_3307|Compound:9126885|FD-Score:3.32|P-value:1.18E-4 ID:SGTC_3329|Compound:9141233|FD-Score:2.84|P-value:8.28E-4

Top fitness defect scores for YDR328C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2563 tomatine 8.27 8.5 µM 2.08E-20 1 6.44E-17 0.87
2 SGTC_1959 st077091 6.53 55.8 µM 2.11E-13 RPP1 & pyrimidine depletion 11 3.20E-11 0.12
3 SGTC_2794 7785382 5.75 27.3 µM 9.37E-11 5 4.58E-9 0.33
4 SGTC_564 3555-0129 5.63 330.0 µM 2.16E-10 1 9.04E-9 1.69
5 SGTC_3214 9129694 5.61 49.5 µM 2.51E-10 1 1.02E-8 0.66
6 SGTC_2450 5739737 5.54 12.9 µM 4.18E-10 1 1.55E-8 0.83
7 SGTC_2288 7946289 5.08 167.3 µM 8.83E-9 2 1.87E-7 0.12
8 SGTC_307 1501-1223 4.60 22.3 µM 1.77E-7 2 2.16E-6 0.08
9 SGTC_370 0180-0345 4.42 38.7 µM 4.78E-7 DNA intercalators 4 4.87E-6 0.14
10 SGTC_1318 1280-3875 4.32 124.0 µM 8.64E-7 4 7.90E-6 0.09
11 SGTC_1597 7-benzyloxygramine 4.29 47.5 µM 1.01E-6 calcium & mitochondrial duress 49 8.96E-6 0.02
12 SGTC_827 3253-0474 4.25 693.0 µM 1.24E-6 4 1.07E-5 0.02
13 SGTC_2003 4023581 4.11 200.0 µM 2.71E-6 cell wall signaling 5 2.01E-5 0.12
14 SGTC_206 4546-0025 4.08 72.0 µM 3.06E-6 NEO1 10 2.23E-5 0.04
15 SGTC_339 3091-4833 3.98 71.4 µM 5.25E-6 iron homeostasis 13 3.47E-5 0.02
16 SGTC_297 0986-0032 3.92 24.0 µM 7.18E-6 60S ribosome export 3 4.48E-5 0.15
17 SGTC_995 3931-2021 3.80 34.6 µM 1.27E-5 RPP1 & pyrimidine depletion 7 7.13E-5 0.20
18 SGTC_276 colchicine 3.69 750.0 µM 2.16E-5 3 1.10E-4 0.21
19 SGTC_816 0325-0417 3.69 81.3 µM 2.20E-5 6 1.12E-4 0.03
20 SGTC_23 0213-0011 3.64 40.0 µM 2.73E-5 copper-dependent oxidative stress 6 1.34E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 3.17E-16 YDR487C RIB3 het 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
0.135 5.06E-15 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.125 4.21E-13 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.123 8.06E-13 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.120 2.86E-12 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.113 4.45E-11 YHR118C ORC6 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
0.107 4.89E-10 YFR006W_p YFR006W_p hom Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
0.105 9.60E-10 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.102 3.70E-9 YGR153W_p YGR153W_p hom Putative protein of unknown function
0.101 4.05E-9 YFL009W CDC4 het F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.101 4.83E-9 YDR299W BFR2 het Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
0.100 5.68E-9 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.097 1.63E-8 YDR327W_d YDR327W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
0.096 2.47E-8 YER093C TSC11 het Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
0.095 3.96E-8 YNL212W VID27 hom Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth