YDR331W / GPI8

ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR331W / GPI8

Click on Significant Values for Screen Details ID:SGTC_168|Compound:3970-0862|FD-Score:4.26|P-value:2.07E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.52|P-value:3.57E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:3.42|P-value:5.08E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.35|P-value:6.41E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.57|P-value:2.98E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.50|P-value:3.29E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.01|P-value:5.62E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:4.00|P-value:5.83E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.36|P-value:6.20E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.21|P-value:9.07E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.25|P-value:8.94E-4 ID:SGTC_847|Compound:0342-0274|FD-Score:4.73|P-value:2.56E-6 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.47|P-value:4.27E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.94|P-value:7.52E-5 ID:SGTC_1355|Compound:1499-0035|FD-Score:-5.33|P-value:1.16E-7 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.73|P-value:1.66E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-4.43|P-value:8.52E-6 ID:SGTC_1453|Compound:4237-0541|FD-Score:-3.19|P-value:9.64E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.45|P-value:9.21E-6 ID:SGTC_1771|Compound:st048882|FD-Score:-4.22|P-value:2.08E-5 ID:SGTC_1898|Compound:st060199|FD-Score:3.78|P-value:1.38E-4 ID:SGTC_1947|Compound:st076332|FD-Score:3.76|P-value:1.48E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.77|P-value:2.13E-6 ID:SGTC_2017|Compound:5132927|FD-Score:5.08|P-value:4.91E-7 ID:SGTC_2018|Compound:5133068|FD-Score:-4.30|P-value:1.44E-5 ID:SGTC_2049|Compound:5262831|FD-Score:3.39|P-value:5.49E-4 ID:SGTC_2050|Compound:5263056|FD-Score:13.00|P-value:2.87E-36 ID:SGTC_2059|Compound:5238311|FD-Score:-3.86|P-value:8.88E-5 ID:SGTC_2336|Compound:9001819|FD-Score:-4.63|P-value:3.43E-6 ID:SGTC_2348|Compound:9022543|FD-Score:-3.79|P-value:1.15E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.33|P-value:5.98E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.35|P-value:6.44E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.95|P-value:7.06E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.24|P-value:9.24E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.86|P-value:8.79E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.48|P-value:3.51E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.70|P-value:1.86E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.74|P-value:1.60E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.48|P-value:4.05E-4 ID:SGTC_3230|Compound:9131665|FD-Score:3.29|P-value:7.70E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.26|P-value:8.57E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.37|P-value:6.07E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-3.57|P-value:2.62E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.26|P-value:2.07E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.52|P-value:3.57E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:3.42|P-value:5.08E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.35|P-value:6.41E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.57|P-value:2.98E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.50|P-value:3.29E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.01|P-value:5.62E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:4.00|P-value:5.83E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.36|P-value:6.20E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.21|P-value:9.07E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.25|P-value:8.94E-4 ID:SGTC_847|Compound:0342-0274|FD-Score:4.73|P-value:2.56E-6 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.47|P-value:4.27E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.94|P-value:7.52E-5 ID:SGTC_1355|Compound:1499-0035|FD-Score:-5.33|P-value:1.16E-7 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.73|P-value:1.66E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-4.43|P-value:8.52E-6 ID:SGTC_1453|Compound:4237-0541|FD-Score:-3.19|P-value:9.64E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.45|P-value:9.21E-6 ID:SGTC_1771|Compound:st048882|FD-Score:-4.22|P-value:2.08E-5 ID:SGTC_1898|Compound:st060199|FD-Score:3.78|P-value:1.38E-4 ID:SGTC_1947|Compound:st076332|FD-Score:3.76|P-value:1.48E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.77|P-value:2.13E-6 ID:SGTC_2017|Compound:5132927|FD-Score:5.08|P-value:4.91E-7 ID:SGTC_2018|Compound:5133068|FD-Score:-4.30|P-value:1.44E-5 ID:SGTC_2049|Compound:5262831|FD-Score:3.39|P-value:5.49E-4 ID:SGTC_2050|Compound:5263056|FD-Score:13.00|P-value:2.87E-36 ID:SGTC_2059|Compound:5238311|FD-Score:-3.86|P-value:8.88E-5 ID:SGTC_2336|Compound:9001819|FD-Score:-4.63|P-value:3.43E-6 ID:SGTC_2348|Compound:9022543|FD-Score:-3.79|P-value:1.15E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.33|P-value:5.98E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.35|P-value:6.44E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.95|P-value:7.06E-5 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.24|P-value:9.24E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.86|P-value:8.79E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.48|P-value:3.51E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.70|P-value:1.86E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.74|P-value:1.60E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.48|P-value:4.05E-4 ID:SGTC_3230|Compound:9131665|FD-Score:3.29|P-value:7.70E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.26|P-value:8.57E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.37|P-value:6.07E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-3.57|P-value:2.62E-4

Top fitness defect scores for YDR331W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2050 5263056 13.00 171.0 µM 2.87E-36 fatty acid desaturase (OLE1) 2 9.64E-39 3.07
2 SGTC_2017 5132927 5.08 60.6 µM 4.91E-7 1 1.88E-7 0.54
3 SGTC_2013 4024628 4.77 200.0 µM 2.13E-6 6 9.02E-7 0.00
4 SGTC_847 0342-0274 4.73 5.5 µM 2.56E-6 1 1.10E-6 0.54
5 SGTC_1465 k213-0056 4.45 144.0 µM 9.21E-6 DNA intercalators 6 4.33E-6 0.12
6 SGTC_168 3970-0862 4.26 530.5 µM 2.07E-5 39 1.03E-5 0.00
7 SGTC_513 4245-1575 4.01 41.7 µM 5.62E-5 unfolded protein response 28 3.00E-5 0.06
8 SGTC_626 0214-0009 4.00 33.0 µM 5.83E-5 copper-dependent oxidative stress 2 3.12E-5 0.13
9 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 3.95 100.0 µM 7.06E-5 8 3.83E-5 0.28
10 SGTC_1059 3448-6867 3.94 171.0 µM 7.52E-5 4 4.10E-5 0.07
11 SGTC_1898 st060199 3.78 18.6 µM 1.38E-4 6 7.85E-5 0.07
12 SGTC_1947 st076332 3.76 61.6 µM 1.48E-4 4 8.43E-5 0.00
13 SGTC_2974 9094440 3.74 9.7 µM 1.60E-4 ergosterol biosynthesis 16 9.22E-5 0.13
14 SGTC_1384 3089-0840 3.73 101.0 µM 1.66E-4 1 9.57E-5 0.40
15 SGTC_2957 9076800 3.70 11.5 µM 1.86E-4 6 1.08E-4 0.16
16 SGTC_412 gemfibrozil 3.57 100.0 µM 2.98E-4 DNA intercalators 10 1.79E-4 0.17
17 SGTC_261 5-fluorouridine 3.52 15.4 µM 3.57E-4 exosome 17 2.17E-4 0.17
18 SGTC_2991 9074669 3.48 71.4 µM 4.05E-4 azole & statin 29 2.49E-4 0.10
19 SGTC_1055 3448-0672 3.47 86.0 µM 4.27E-4 20 2.63E-4 0.03
20 SGTC_344 k064-0035 3.42 6.4 µM 5.08E-4 60S ribosome export 10 3.16E-4 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.198 4.05E-31 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
0.126 2.22E-13 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.123 9.21E-13 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.122 1.51E-12 YBR190W_d YBR190W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
0.120 3.42E-12 YBR087W RFC5 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.118 6.60E-12 YLR349W_d YLR349W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
0.114 3.97E-11 YBR063C_p YBR063C_p hom Putative protein of unknown function; YBR063C is not an essential gene
0.111 1.32E-10 YOR232W MGE1 het Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
0.110 1.48E-10 YGL260W_p YGL260W_p hom Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium
0.110 2.02E-10 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.109 2.72E-10 YER140W EMP65 hom ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.108 3.17E-10 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.108 3.74E-10 YDL051W LHP1 hom RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
0.100 6.13E-9 YPL143W RPL33A het Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
0.100 6.18E-9 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis