YDR332W / IRC3

Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR332W / IRC3

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.90|P-value:8.05E-7 ID:SGTC_19|Compound:0279-0214|FD-Score:3.23|P-value:7.79E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.95|P-value:5.50E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:5.28|P-value:1.19E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:4.41|P-value:7.68E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:3.79|P-value:1.04E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.54|P-value:2.63E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:3.90|P-value:6.55E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:4.55|P-value:4.18E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:-6.38|P-value:2.59E-10 ID:SGTC_173|Compound:1273-0061|FD-Score:5.73|P-value:1.05E-8 ID:SGTC_236|Compound:1509-0011|FD-Score:16.50|P-value:5.52E-59 ID:SGTC_275|Compound:trichlorophene|FD-Score:6.45|P-value:1.41E-10 ID:SGTC_310|Compound:1273-0058|FD-Score:6.36|P-value:2.39E-10 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.64|P-value:2.02E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:4.46|P-value:6.16E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.44|P-value:4.17E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:-4.10|P-value:3.36E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:3.95|P-value:5.37E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.93|P-value:6.52E-5 ID:SGTC_659|Compound:k018-0002|FD-Score:-3.37|P-value:5.31E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:-3.22|P-value:8.85E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.32|P-value:5.73E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.44|P-value:3.72E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.29|P-value:6.31E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:-3.71|P-value:1.53E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:5.84|P-value:5.54E-9 ID:SGTC_899|Compound:1083-0020|FD-Score:3.28|P-value:6.59E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:6.09|P-value:1.31E-9 ID:SGTC_961|Compound:1252-1571|FD-Score:3.54|P-value:2.58E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:4.26|P-value:1.50E-5 ID:SGTC_978|Compound:1416-0512|FD-Score:-3.61|P-value:2.24E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.89|P-value:8.30E-7 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.94|P-value:5.65E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.76|P-value:1.15E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.89|P-value:8.49E-7 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.52|P-value:2.75E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:-3.40|P-value:4.72E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.68|P-value:1.55E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:5.50|P-value:3.59E-8 ID:SGTC_1377|Compound:2944-0135|FD-Score:-3.46|P-value:3.90E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.42|P-value:4.05E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:-4.60|P-value:3.78E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:-5.53|P-value:3.73E-8 ID:SGTC_1529|Compound:apigenin|FD-Score:4.10|P-value:2.94E-5 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.49|P-value:3.12E-4 ID:SGTC_1621|Compound:st003705|FD-Score:4.28|P-value:1.39E-5 ID:SGTC_1622|Compound:st003707|FD-Score:3.17|P-value:9.49E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:4.67|P-value:2.37E-6 ID:SGTC_1707|Compound:st032323|FD-Score:-4.47|P-value:6.95E-6 ID:SGTC_1742|Compound:st038235|FD-Score:5.02|P-value:4.53E-7 ID:SGTC_1764|Compound:st044502|FD-Score:3.22|P-value:7.97E-4 ID:SGTC_1774|Compound:st048371|FD-Score:-3.30|P-value:6.62E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.61|P-value:2.30E-8 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:4.16|P-value:2.35E-5 ID:SGTC_1921|Compound:5740136|FD-Score:6.41|P-value:1.84E-10 ID:SGTC_1922|Compound:5578187|FD-Score:3.15|P-value:0.00100 ID:SGTC_2030|Compound:5175110|FD-Score:3.33|P-value:5.41E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.73|P-value:1.29E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.41|P-value:4.15E-4 ID:SGTC_2157|Compound:5649910|FD-Score:-3.89|P-value:7.79E-5 ID:SGTC_2165|Compound:5727197|FD-Score:4.26|P-value:1.48E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.30|P-value:6.16E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.34|P-value:5.37E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.35|P-value:5.71E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.60|P-value:3.36E-6 ID:SGTC_2282|Compound:7944268|FD-Score:3.23|P-value:7.62E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.55|P-value:2.55E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-3.20|P-value:9.26E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.65|P-value:1.72E-4 ID:SGTC_2325|Compound:6158266|FD-Score:5.24|P-value:1.48E-7 ID:SGTC_2353|Compound:9059630|FD-Score:3.38|P-value:4.54E-4 ID:SGTC_2440|Compound:5628896|FD-Score:-5.44|P-value:6.08E-8 ID:SGTC_2449|Compound:5812982|FD-Score:-4.07|P-value:3.72E-5 ID:SGTC_2462|Compound:5162487|FD-Score:3.21|P-value:8.26E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-3.56|P-value:2.71E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.40|P-value:9.31E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.20|P-value:8.57E-4 ID:SGTC_2793|Compound:7325131|FD-Score:-4.41|P-value:8.85E-6 ID:SGTC_2801|Compound:7907800|FD-Score:-4.57|P-value:4.40E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-5.40|P-value:7.18E-8 ID:SGTC_2960|Compound:9079389|FD-Score:3.53|P-value:2.71E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.37|P-value:4.82E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-4.05|P-value:4.06E-5 ID:SGTC_3202|Compound:9113761|FD-Score:-3.67|P-value:1.80E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.38|P-value:5.09E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-3.40|P-value:4.84E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.18|P-value:9.15E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.39|P-value:4.51E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.90|P-value:8.05E-7 ID:SGTC_19|Compound:0279-0214|FD-Score:3.23|P-value:7.79E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.95|P-value:5.50E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:5.28|P-value:1.19E-7 ID:SGTC_63|Compound:0099-0242|FD-Score:4.41|P-value:7.68E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:3.79|P-value:1.04E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.54|P-value:2.63E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:3.90|P-value:6.55E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:4.55|P-value:4.18E-6 ID:SGTC_151|Compound:3013-0144|FD-Score:-6.38|P-value:2.59E-10 ID:SGTC_173|Compound:1273-0061|FD-Score:5.73|P-value:1.05E-8 ID:SGTC_236|Compound:1509-0011|FD-Score:16.50|P-value:5.52E-59 ID:SGTC_275|Compound:trichlorophene|FD-Score:6.45|P-value:1.41E-10 ID:SGTC_310|Compound:1273-0058|FD-Score:6.36|P-value:2.39E-10 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.64|P-value:2.02E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:4.46|P-value:6.16E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.44|P-value:4.17E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:-4.10|P-value:3.36E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:3.95|P-value:5.37E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.93|P-value:6.52E-5 ID:SGTC_659|Compound:k018-0002|FD-Score:-3.37|P-value:5.31E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:-3.22|P-value:8.85E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.32|P-value:5.73E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.44|P-value:3.72E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.29|P-value:6.31E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:-3.71|P-value:1.53E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:5.84|P-value:5.54E-9 ID:SGTC_899|Compound:1083-0020|FD-Score:3.28|P-value:6.59E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:6.09|P-value:1.31E-9 ID:SGTC_961|Compound:1252-1571|FD-Score:3.54|P-value:2.58E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:4.26|P-value:1.50E-5 ID:SGTC_978|Compound:1416-0512|FD-Score:-3.61|P-value:2.24E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.89|P-value:8.30E-7 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.94|P-value:5.65E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.76|P-value:1.15E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.89|P-value:8.49E-7 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.52|P-value:2.75E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:-3.40|P-value:4.72E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.68|P-value:1.55E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:5.50|P-value:3.59E-8 ID:SGTC_1377|Compound:2944-0135|FD-Score:-3.46|P-value:3.90E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.42|P-value:4.05E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:-4.60|P-value:3.78E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:-5.53|P-value:3.73E-8 ID:SGTC_1529|Compound:apigenin|FD-Score:4.10|P-value:2.94E-5 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.49|P-value:3.12E-4 ID:SGTC_1621|Compound:st003705|FD-Score:4.28|P-value:1.39E-5 ID:SGTC_1622|Compound:st003707|FD-Score:3.17|P-value:9.49E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:4.67|P-value:2.37E-6 ID:SGTC_1707|Compound:st032323|FD-Score:-4.47|P-value:6.95E-6 ID:SGTC_1742|Compound:st038235|FD-Score:5.02|P-value:4.53E-7 ID:SGTC_1764|Compound:st044502|FD-Score:3.22|P-value:7.97E-4 ID:SGTC_1774|Compound:st048371|FD-Score:-3.30|P-value:6.62E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.61|P-value:2.30E-8 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:4.16|P-value:2.35E-5 ID:SGTC_1921|Compound:5740136|FD-Score:6.41|P-value:1.84E-10 ID:SGTC_1922|Compound:5578187|FD-Score:3.15|P-value:0.00100 ID:SGTC_2030|Compound:5175110|FD-Score:3.33|P-value:5.41E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.73|P-value:1.29E-4 ID:SGTC_2139|Compound:5578692|FD-Score:3.41|P-value:4.15E-4 ID:SGTC_2157|Compound:5649910|FD-Score:-3.89|P-value:7.79E-5 ID:SGTC_2165|Compound:5727197|FD-Score:4.26|P-value:1.48E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.30|P-value:6.16E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.34|P-value:5.37E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.35|P-value:5.71E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.60|P-value:3.36E-6 ID:SGTC_2282|Compound:7944268|FD-Score:3.23|P-value:7.62E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.55|P-value:2.55E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-3.20|P-value:9.26E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.65|P-value:1.72E-4 ID:SGTC_2325|Compound:6158266|FD-Score:5.24|P-value:1.48E-7 ID:SGTC_2353|Compound:9059630|FD-Score:3.38|P-value:4.54E-4 ID:SGTC_2440|Compound:5628896|FD-Score:-5.44|P-value:6.08E-8 ID:SGTC_2449|Compound:5812982|FD-Score:-4.07|P-value:3.72E-5 ID:SGTC_2462|Compound:5162487|FD-Score:3.21|P-value:8.26E-4 ID:SGTC_2482|Compound:5981709|FD-Score:-3.56|P-value:2.71E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.40|P-value:9.31E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.20|P-value:8.57E-4 ID:SGTC_2793|Compound:7325131|FD-Score:-4.41|P-value:8.85E-6 ID:SGTC_2801|Compound:7907800|FD-Score:-4.57|P-value:4.40E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-5.40|P-value:7.18E-8 ID:SGTC_2960|Compound:9079389|FD-Score:3.53|P-value:2.71E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.37|P-value:4.82E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-4.05|P-value:4.06E-5 ID:SGTC_3202|Compound:9113761|FD-Score:-3.67|P-value:1.80E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.38|P-value:5.09E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-3.40|P-value:4.84E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.18|P-value:9.15E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.39|P-value:4.51E-4

Top fitness defect scores for YDR332W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 16.50 5.3 µM 5.52E-59 83 1.08E-61
2 SGTC_275 trichlorophene 6.45 5.7 µM 1.41E-10 mitochondrial stress 102 5.68E-11
3 SGTC_1921 5740136 6.41 50.0 µM 1.84E-10 TRP & mitochondrial translation 13 7.50E-11
4 SGTC_310 1273-0058 6.36 1.1 µM 2.39E-10 mitochondrial stress 134 9.83E-11
5 SGTC_908 3448-7491 6.09 47.7 µM 1.31E-9 mitochondrial stress 46 5.82E-10
6 SGTC_748 0136-0228 5.84 77.9 µM 5.54E-9 redox potentiating 39 2.64E-9
7 SGTC_173 1273-0061 5.73 2.7 µM 1.05E-8 mitochondrial stress 117 5.14E-9
8 SGTC_1373 2887-0003 5.50 58.5 µM 3.59E-8 Golgi 4 1.86E-8
9 SGTC_54 0159-0055 5.28 9.0 µM 1.19E-7 ERG2 26 6.53E-8
10 SGTC_2325 6158266 5.24 200.0 µM 1.48E-7 7 8.20E-8
11 SGTC_1742 st038235 5.02 28.9 µM 4.53E-7 mitochondrial stress 73 2.63E-7
12 SGTC_10 0279-0212 4.90 42.6 µM 8.05E-7 TRP & mitochondrial translation 67 4.80E-7
13 SGTC_1129 3453-1384 4.89 223.0 µM 8.30E-7 21 4.96E-7
14 SGTC_1188 1403-0014 4.89 4.6 µM 8.49E-7 mitochondrial stress 29 5.08E-7
15 SGTC_1631 benzylvanillin 4.67 81.9 µM 2.37E-6 Golgi 14 1.49E-6
16 SGTC_2250 alverine citrate 4.60 130.0 µM 3.36E-6 116 2.14E-6
17 SGTC_87 0100-0052 4.55 35.5 µM 4.18E-6 71 2.68E-6
18 SGTC_365 0121-0052 4.46 13.6 µM 6.16E-6 mitochondrial response to ROS 42 4.02E-6
19 SGTC_63 0099-0242 4.41 26.7 µM 7.68E-6 mitochondrial stress 75 5.06E-6
20 SGTC_1621 st003705 4.28 32.4 µM 1.39E-5 calcium & mitochondrial duress 18 9.41E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.494 9.21E-205 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.459 4.07E-174 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.456 1.58E-171 YGR102C GTF1 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
0.451 4.53E-167 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
0.446 1.34E-163 YDR079W PET100 hom Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
0.446 1.84E-163 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.442 1.21E-160 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.436 1.09E-155 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.433 2.84E-153 YOL095C HMI1 hom Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.432 5.08E-153 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.430 6.65E-151 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit
0.426 2.09E-148 YDR268W MSW1 hom Mitochondrial tryptophanyl-tRNA synthetase
0.421 1.81E-144 YNL184C_p YNL184C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.419 7.39E-143 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.417 1.27E-141 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation