YDR335W / MSN5

Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR335W / MSN5

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:3.70|P-value:2.53E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:17.30|P-value:7.25E-60 ID:SGTC_64|Compound:000l-0018|FD-Score:-4.45|P-value:1.22E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:6.17|P-value:3.24E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:3.83|P-value:1.60E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.86|P-value:1.25E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.77|P-value:2.01E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:12.50|P-value:1.52E-32 ID:SGTC_141|Compound:0443-0274|FD-Score:4.83|P-value:2.76E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:4.75|P-value:3.89E-6 ID:SGTC_156|Compound:3474-0145|FD-Score:3.88|P-value:1.33E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:8.33|P-value:2.13E-15 ID:SGTC_278|Compound:4092-0821|FD-Score:3.89|P-value:1.30E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:-3.39|P-value:6.47E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.78|P-value:2.60E-8 ID:SGTC_320|Compound:4092-0846|FD-Score:4.23|P-value:3.46E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.75|P-value:3.94E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:8.37|P-value:1.53E-15 ID:SGTC_504|Compound:lovastatin|FD-Score:6.86|P-value:5.10E-11 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.55|P-value:7.87E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:12.20|P-value:3.84E-31 ID:SGTC_521|Compound:r062-0005|FD-Score:3.46|P-value:5.78E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.24|P-value:2.12E-9 ID:SGTC_582|Compound:0987-0078|FD-Score:3.92|P-value:1.15E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:3.34|P-value:8.60E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:4.27|P-value:3.00E-5 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.33|P-value:8.99E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.46|P-value:5.85E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.99|P-value:1.33E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:3.42|P-value:6.72E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.53|P-value:1.03E-5 ID:SGTC_1632|Compound:st005650|FD-Score:4.28|P-value:2.83E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.92|P-value:1.24E-8 ID:SGTC_1729|Compound:st035615|FD-Score:4.31|P-value:2.53E-5 ID:SGTC_1730|Compound:st033808|FD-Score:4.97|P-value:1.46E-6 ID:SGTC_1744|Compound:st037937|FD-Score:3.80|P-value:1.80E-4 ID:SGTC_1781|Compound:st038725|FD-Score:6.48|P-value:5.14E-10 ID:SGTC_1808|Compound:st050063|FD-Score:3.38|P-value:7.67E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.01|P-value:6.92E-5 ID:SGTC_1966|Compound:st070145|FD-Score:4.26|P-value:3.15E-5 ID:SGTC_2008|Compound:4012253|FD-Score:-3.30|P-value:8.64E-4 ID:SGTC_2090|Compound:5301565|FD-Score:-3.92|P-value:9.87E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:7.84|P-value:7.39E-14 ID:SGTC_2679|Compound:rapamycin|FD-Score:-5.25|P-value:3.17E-7 ID:SGTC_2788|Compound:5282734|FD-Score:-4.39|P-value:1.55E-5 ID:SGTC_2912|Compound:7964298|FD-Score:4.29|P-value:2.71E-5 ID:SGTC_3031|Compound:9088762|FD-Score:3.65|P-value:3.02E-4 ID:SGTC_3037|Compound:9091026|FD-Score:6.98|P-value:2.49E-11 ID:SGTC_3120|Compound:9124077|FD-Score:3.44|P-value:6.18E-4 ID:SGTC_3135|Compound:9127742|FD-Score:-3.38|P-value:6.61E-4 ID:SGTC_3149|Compound:9097855|FD-Score:-3.56|P-value:3.58E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.47|P-value:1.32E-5 ID:SGTC_3189|Compound:9110682|FD-Score:5.56|P-value:8.22E-8 ID:SGTC_3198|Compound:9112019|FD-Score:3.81|P-value:1.71E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.65|P-value:2.60E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.65|P-value:3.06E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.91|P-value:1.02E-4 ID:SGTC_3304|Compound:9125678|FD-Score:5.04|P-value:1.08E-6 ID:SGTC_29|Compound:0299-0004|FD-Score:3.70|P-value:2.53E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:17.30|P-value:7.25E-60 ID:SGTC_64|Compound:000l-0018|FD-Score:-4.45|P-value:1.22E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:6.17|P-value:3.24E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:3.83|P-value:1.60E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.86|P-value:1.25E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.77|P-value:2.01E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:12.50|P-value:1.52E-32 ID:SGTC_141|Compound:0443-0274|FD-Score:4.83|P-value:2.76E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:4.75|P-value:3.89E-6 ID:SGTC_156|Compound:3474-0145|FD-Score:3.88|P-value:1.33E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:8.33|P-value:2.13E-15 ID:SGTC_278|Compound:4092-0821|FD-Score:3.89|P-value:1.30E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:-3.39|P-value:6.47E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.78|P-value:2.60E-8 ID:SGTC_320|Compound:4092-0846|FD-Score:4.23|P-value:3.46E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.75|P-value:3.94E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:8.37|P-value:1.53E-15 ID:SGTC_504|Compound:lovastatin|FD-Score:6.86|P-value:5.10E-11 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.55|P-value:7.87E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:12.20|P-value:3.84E-31 ID:SGTC_521|Compound:r062-0005|FD-Score:3.46|P-value:5.78E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.24|P-value:2.12E-9 ID:SGTC_582|Compound:0987-0078|FD-Score:3.92|P-value:1.15E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:3.34|P-value:8.60E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:4.27|P-value:3.00E-5 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.33|P-value:8.99E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.46|P-value:5.85E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.99|P-value:1.33E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:3.42|P-value:6.72E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.53|P-value:1.03E-5 ID:SGTC_1632|Compound:st005650|FD-Score:4.28|P-value:2.83E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.92|P-value:1.24E-8 ID:SGTC_1729|Compound:st035615|FD-Score:4.31|P-value:2.53E-5 ID:SGTC_1730|Compound:st033808|FD-Score:4.97|P-value:1.46E-6 ID:SGTC_1744|Compound:st037937|FD-Score:3.80|P-value:1.80E-4 ID:SGTC_1781|Compound:st038725|FD-Score:6.48|P-value:5.14E-10 ID:SGTC_1808|Compound:st050063|FD-Score:3.38|P-value:7.67E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.01|P-value:6.92E-5 ID:SGTC_1966|Compound:st070145|FD-Score:4.26|P-value:3.15E-5 ID:SGTC_2008|Compound:4012253|FD-Score:-3.30|P-value:8.64E-4 ID:SGTC_2090|Compound:5301565|FD-Score:-3.92|P-value:9.87E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:7.84|P-value:7.39E-14 ID:SGTC_2679|Compound:rapamycin|FD-Score:-5.25|P-value:3.17E-7 ID:SGTC_2788|Compound:5282734|FD-Score:-4.39|P-value:1.55E-5 ID:SGTC_2912|Compound:7964298|FD-Score:4.29|P-value:2.71E-5 ID:SGTC_3031|Compound:9088762|FD-Score:3.65|P-value:3.02E-4 ID:SGTC_3037|Compound:9091026|FD-Score:6.98|P-value:2.49E-11 ID:SGTC_3120|Compound:9124077|FD-Score:3.44|P-value:6.18E-4 ID:SGTC_3135|Compound:9127742|FD-Score:-3.38|P-value:6.61E-4 ID:SGTC_3149|Compound:9097855|FD-Score:-3.56|P-value:3.58E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.47|P-value:1.32E-5 ID:SGTC_3189|Compound:9110682|FD-Score:5.56|P-value:8.22E-8 ID:SGTC_3198|Compound:9112019|FD-Score:3.81|P-value:1.71E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-3.65|P-value:2.60E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.65|P-value:3.06E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.91|P-value:1.02E-4 ID:SGTC_3304|Compound:9125678|FD-Score:5.04|P-value:1.08E-6

Top fitness defect scores for YDR335W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 17.30 9.0 µM 7.25E-60 cell wall 21 5.34E-67
2 SGTC_138 0850-0259 12.50 40.9 µM 1.52E-32 cell wall 24 2.37E-36
3 SGTC_514 4466-0038 12.20 2.6 nM 3.84E-31 cell wall 16 8.92E-35
4 SGTC_467 ro 31-8220 8.37 72.8 µM 1.53E-15 calcium & mitochondrial duress 14 2.83E-17
5 SGTC_157 1348-1695 8.33 65.8 µM 2.13E-15 iron homeostasis 14 4.09E-17
6 SGTC_2582 agaric acid 7.84 100.0 µM 7.39E-14 redox potentiating 107 2.19E-15
7 SGTC_3037 9091026 6.98 49.5 µM 2.49E-11 NEO1-PIK1 29 1.50E-12
8 SGTC_504 lovastatin 6.86 124.0 µM 5.10E-11 1 3.34E-12
9 SGTC_1781 st038725 6.48 66.3 µM 5.14E-10 NEO1 49 4.46E-11
10 SGTC_528 3492-0019 6.24 172.0 µM 2.12E-9 calcium & mitochondrial duress 115 2.18E-10
11 SGTC_65 0798-0905 6.17 35.0 µM 3.24E-9 calcium & mitochondrial duress 29 3.51E-10
12 SGTC_1712 st032288 5.92 53.4 µM 1.24E-8 calcium & mitochondrial duress 78 1.59E-9
13 SGTC_312 4092-0653 5.78 6.8 µM 2.60E-8 ergosterol depletion effects on membrane 42 3.63E-9
14 SGTC_3189 9110682 5.56 49.5 µM 8.22E-8 14 1.32E-8
15 SGTC_3304 9125678 5.04 46.1 µM 1.08E-6 22 2.38E-7
16 SGTC_1310 1188-0297 4.99 994.0 nM 1.33E-6 azole & statin 17 3.00E-7
17 SGTC_1730 st033808 4.97 45.1 µM 1.46E-6 12 3.32E-7
18 SGTC_141 0443-0274 4.83 32.3 µM 2.76E-6 mitochondrial response to ROS 24 6.77E-7
19 SGTC_146 3448-8650 4.75 53.1 µM 3.89E-6 NEO1-PIK1 96 9.94E-7
20 SGTC_351 caspofungin 4.75 25.0 nM 3.94E-6 cell wall 62 1.01E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.391 5.54E-123 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.366 5.78E-107 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.359 7.05E-103 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.336 2.58E-89 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.324 1.25E-82 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.321 2.95E-81 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.318 1.01E-79 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.318 1.40E-79 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.317 2.86E-79 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.316 7.66E-79 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.315 2.70E-78 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.310 2.25E-75 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.309 2.70E-75 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.304 8.74E-73 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.301 2.37E-71 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP