YDR348C / PAL1

Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR348C / PAL1

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:3.79|P-value:5.78E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.82|P-value:5.11E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:4.84|P-value:4.11E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:10.20|P-value:9.03E-26 ID:SGTC_212|Compound:3807-4682|FD-Score:3.10|P-value:8.06E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.43|P-value:2.40E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.13|P-value:7.47E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.28|P-value:4.28E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.50|P-value:1.87E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.76|P-value:5.29E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.26|P-value:4.64E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.31|P-value:3.22E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.58|P-value:1.11E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.46|P-value:2.21E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.27|P-value:3.73E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:14.80|P-value:6.15E-52 ID:SGTC_518|Compound:1021-0119|FD-Score:4.53|P-value:2.02E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:6.91|P-value:8.65E-13 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.13|P-value:6.21E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.11|P-value:7.75E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:4.02|P-value:2.14E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.27|P-value:4.52E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:4.82|P-value:4.47E-7 ID:SGTC_921|Compound:1561-0023|FD-Score:3.36|P-value:3.14E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:5.76|P-value:2.09E-9 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.27|P-value:7.02E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:4.02|P-value:2.17E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.26|P-value:4.67E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:4.40|P-value:3.79E-6 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.28|P-value:4.26E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:-3.44|P-value:1.95E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:5.83|P-value:1.39E-9 ID:SGTC_1346|Compound:1488-2224|FD-Score:7.37|P-value:2.77E-14 ID:SGTC_1352|Compound:1496-0991|FD-Score:-3.82|P-value:4.25E-5 ID:SGTC_1355|Compound:1499-0035|FD-Score:-3.04|P-value:8.63E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.66|P-value:9.73E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:7.52|P-value:8.36E-15 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.50|P-value:2.29E-6 ID:SGTC_1523|Compound:st012844|FD-Score:6.60|P-value:8.44E-12 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:-3.68|P-value:7.39E-5 ID:SGTC_1526|Compound:st051518|FD-Score:-3.03|P-value:8.91E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.65|P-value:1.01E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.23|P-value:5.15E-4 ID:SGTC_1751|Compound:st044839|FD-Score:3.14|P-value:7.19E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.65|P-value:1.01E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.52|P-value:1.69E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.13|P-value:7.32E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.24|P-value:5.02E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.27|P-value:4.51E-4 ID:SGTC_2210|Compound:7115885|FD-Score:4.96|P-value:2.15E-7 ID:SGTC_2241|Compound:6772625|FD-Score:-3.05|P-value:8.31E-4 ID:SGTC_2289|Compound:7946404|FD-Score:8.24|P-value:1.98E-17 ID:SGTC_2304|Compound:7443016|FD-Score:3.55|P-value:1.54E-4 ID:SGTC_2309|Compound:7664088|FD-Score:7.05|P-value:3.21E-13 ID:SGTC_2396|Compound:5342950|FD-Score:4.54|P-value:1.88E-6 ID:SGTC_2447|Compound:5741742|FD-Score:3.14|P-value:7.07E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.32|P-value:5.54E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:4.61|P-value:1.35E-6 ID:SGTC_2801|Compound:7907800|FD-Score:-3.73|P-value:6.19E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.14|P-value:7.19E-4 ID:SGTC_2912|Compound:7964298|FD-Score:19.30|P-value:1.22E-86 ID:SGTC_3009|Compound:9080819|FD-Score:-4.30|P-value:4.77E-6 ID:SGTC_3130|Compound:9127414|FD-Score:-4.02|P-value:1.72E-5 ID:SGTC_3350|Compound:9150499|FD-Score:4.91|P-value:2.90E-7 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.79|P-value:5.78E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.82|P-value:5.11E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:4.84|P-value:4.11E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:10.20|P-value:9.03E-26 ID:SGTC_212|Compound:3807-4682|FD-Score:3.10|P-value:8.06E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.43|P-value:2.40E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.13|P-value:7.47E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.28|P-value:4.28E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.50|P-value:1.87E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.76|P-value:5.29E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.26|P-value:4.64E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.31|P-value:3.22E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.58|P-value:1.11E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.46|P-value:2.21E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.27|P-value:3.73E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:14.80|P-value:6.15E-52 ID:SGTC_518|Compound:1021-0119|FD-Score:4.53|P-value:2.02E-6 ID:SGTC_586|Compound:0957-0265|FD-Score:6.91|P-value:8.65E-13 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.13|P-value:6.21E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.11|P-value:7.75E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:4.02|P-value:2.14E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.27|P-value:4.52E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:4.82|P-value:4.47E-7 ID:SGTC_921|Compound:1561-0023|FD-Score:3.36|P-value:3.14E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:5.76|P-value:2.09E-9 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.27|P-value:7.02E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:4.02|P-value:2.17E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.26|P-value:4.67E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:4.40|P-value:3.79E-6 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.28|P-value:4.26E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:-3.44|P-value:1.95E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:5.83|P-value:1.39E-9 ID:SGTC_1346|Compound:1488-2224|FD-Score:7.37|P-value:2.77E-14 ID:SGTC_1352|Compound:1496-0991|FD-Score:-3.82|P-value:4.25E-5 ID:SGTC_1355|Compound:1499-0035|FD-Score:-3.04|P-value:8.63E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.66|P-value:9.73E-5 ID:SGTC_1361|Compound:1608-0041|FD-Score:7.52|P-value:8.36E-15 ID:SGTC_1362|Compound:1611-0397|FD-Score:4.50|P-value:2.29E-6 ID:SGTC_1523|Compound:st012844|FD-Score:6.60|P-value:8.44E-12 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:-3.68|P-value:7.39E-5 ID:SGTC_1526|Compound:st051518|FD-Score:-3.03|P-value:8.91E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.65|P-value:1.01E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.23|P-value:5.15E-4 ID:SGTC_1751|Compound:st044839|FD-Score:3.14|P-value:7.19E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.65|P-value:1.01E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.52|P-value:1.69E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.13|P-value:7.32E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.24|P-value:5.02E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.27|P-value:4.51E-4 ID:SGTC_2210|Compound:7115885|FD-Score:4.96|P-value:2.15E-7 ID:SGTC_2241|Compound:6772625|FD-Score:-3.05|P-value:8.31E-4 ID:SGTC_2289|Compound:7946404|FD-Score:8.24|P-value:1.98E-17 ID:SGTC_2304|Compound:7443016|FD-Score:3.55|P-value:1.54E-4 ID:SGTC_2309|Compound:7664088|FD-Score:7.05|P-value:3.21E-13 ID:SGTC_2396|Compound:5342950|FD-Score:4.54|P-value:1.88E-6 ID:SGTC_2447|Compound:5741742|FD-Score:3.14|P-value:7.07E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.32|P-value:5.54E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:4.61|P-value:1.35E-6 ID:SGTC_2801|Compound:7907800|FD-Score:-3.73|P-value:6.19E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.14|P-value:7.19E-4 ID:SGTC_2912|Compound:7964298|FD-Score:19.30|P-value:1.22E-86 ID:SGTC_3009|Compound:9080819|FD-Score:-4.30|P-value:4.77E-6 ID:SGTC_3130|Compound:9127414|FD-Score:-4.02|P-value:1.72E-5 ID:SGTC_3350|Compound:9150499|FD-Score:4.91|P-value:2.90E-7

Top fitness defect scores for YDR348C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2912 7964298 19.30 10.9 µM 1.22E-86 cell wall 6 5.63E-83
2 SGTC_514 4466-0038 14.80 2.6 nM 6.15E-52 cell wall 7 8.25E-50
3 SGTC_138 0850-0259 10.20 40.9 µM 9.03E-26 cell wall 43 8.73E-25
4 SGTC_2289 7946404 8.24 165.1 µM 1.98E-17 15 8.40E-17
5 SGTC_1361 1608-0041 7.52 5.6 µM 8.36E-15 RPP1 & pyrimidine depletion 1 2.75E-14
6 SGTC_1346 1488-2224 7.37 62.4 µM 2.77E-14 3 8.67E-14
7 SGTC_2309 7664088 7.05 163.9 µM 3.21E-13 1 9.06E-13
8 SGTC_586 0957-0265 6.91 8.2 µM 8.65E-13 cell wall 11 2.34E-12
9 SGTC_1523 st012844 6.60 44.7 µM 8.44E-12 60S ribosome export 1 2.08E-11
10 SGTC_1317 1273-0028 5.83 910.0 nM 1.39E-9 azole & statin 27 2.78E-9
11 SGTC_964 1287-0107 5.76 19.4 µM 2.09E-9 cell wall 14 4.10E-9
12 SGTC_2210 7115885 4.96 190.4 µM 2.15E-7 cell wall signaling 21 3.50E-7
13 SGTC_3350 9150499 4.91 3.6 µM 2.90E-7 cell wall 27 4.66E-7
14 SGTC_65 0798-0905 4.84 35.0 µM 4.11E-7 calcium & mitochondrial duress 56 6.52E-7
15 SGTC_863 0466-0191 4.82 129.0 µM 4.47E-7 cell wall 47 7.07E-7
16 SGTC_2619 obliquin 4.61 87.9 µM 1.35E-6 cell wall 111 2.04E-6
17 SGTC_2396 5342950 4.54 42.8 µM 1.88E-6 RSC complex & mRNA processing 27 2.81E-6
18 SGTC_518 1021-0119 4.53 62.6 µM 2.02E-6 ERG2 21 3.01E-6
19 SGTC_1362 1611-0397 4.50 101.0 µM 2.29E-6 10 3.39E-6
20 SGTC_1207 2099-0101 4.40 141.0 µM 3.79E-6 7 5.51E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.438 1.51E-157 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.409 9.98E-136 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.374 1.01E-111 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.356 4.69E-101 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.347 1.61E-95 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.333 1.01E-87 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.317 3.57E-79 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.316 1.13E-78 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.315 3.00E-78 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.300 6.31E-71 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.300 8.55E-71 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.289 1.13E-65 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.286 2.66E-64 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.285 1.37E-63 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.283 7.88E-63 YNL233W BNI4 hom Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p