YDR357C_p / CNL1

Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR357C / CNL1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.62|P-value:2.80E-7 ID:SGTC_118|Compound:0083-0097|FD-Score:-2.85|P-value:9.84E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:-3.09|P-value:3.93E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.68|P-value:3.74E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:3.40|P-value:1.24E-4 ID:SGTC_398|Compound:bronopol|FD-Score:4.72|P-value:1.81E-7 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.13|P-value:3.77E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-2.98|P-value:5.96E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.74|P-value:1.65E-7 ID:SGTC_544|Compound:k015-0020|FD-Score:-3.34|P-value:1.42E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-2.91|P-value:7.82E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:4.51|P-value:5.78E-7 ID:SGTC_788|Compound:1182-0090|FD-Score:3.28|P-value:2.09E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:3.10|P-value:4.18E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.13|P-value:3.40E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.20|P-value:3.06E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.21|P-value:2.72E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.54|P-value:6.87E-5 ID:SGTC_1590|Compound:st077108|FD-Score:3.10|P-value:4.17E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.06|P-value:4.89E-4 ID:SGTC_1688|Compound:st021091|FD-Score:4.30|P-value:1.82E-6 ID:SGTC_1712|Compound:st032288|FD-Score:8.86|P-value:5.46E-22 ID:SGTC_2002|Compound:4023503|FD-Score:3.84|P-value:1.76E-5 ID:SGTC_2006|Compound:4011487|FD-Score:3.08|P-value:4.51E-4 ID:SGTC_2021|Compound:4019055|FD-Score:7.20|P-value:3.97E-15 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.47|P-value:9.49E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.28|P-value:2.03E-4 ID:SGTC_2328|Compound:6201408|FD-Score:4.51|P-value:5.74E-7 ID:SGTC_2329|Compound:6138490|FD-Score:-5.10|P-value:1.63E-8 ID:SGTC_2330|Compound:7979373|FD-Score:-3.05|P-value:4.57E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.35|P-value:1.37E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.72|P-value:3.12E-5 ID:SGTC_2453|Compound:5731132|FD-Score:3.24|P-value:2.40E-4 ID:SGTC_2490|Compound:carminic acid|FD-Score:4.44|P-value:8.60E-7 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:-3.36|P-value:1.35E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.69|P-value:3.56E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.52|P-value:6.79E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:3.44|P-value:1.06E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.26|P-value:2.25E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-2.86|P-value:9.64E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.69|P-value:3.13E-5 ID:SGTC_2849|Compound:9019321|FD-Score:6.04|P-value:3.66E-11 ID:SGTC_2856|Compound:9022925|FD-Score:3.12|P-value:3.94E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.33|P-value:1.66E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-4.99|P-value:3.23E-8 ID:SGTC_3037|Compound:9091026|FD-Score:3.28|P-value:2.10E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-3.09|P-value:4.00E-4 ID:SGTC_3075|Compound:9116269|FD-Score:3.30|P-value:1.88E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.50|P-value:7.23E-5 ID:SGTC_3201|Compound:9113999|FD-Score:3.00|P-value:6.10E-4 ID:SGTC_3308|Compound:9128332|FD-Score:3.16|P-value:3.38E-4 ID:SGTC_3334|Compound:9142812|FD-Score:-3.28|P-value:1.88E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.62|P-value:2.80E-7 ID:SGTC_118|Compound:0083-0097|FD-Score:-2.85|P-value:9.84E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:-3.09|P-value:3.93E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.68|P-value:3.74E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:3.40|P-value:1.24E-4 ID:SGTC_398|Compound:bronopol|FD-Score:4.72|P-value:1.81E-7 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.13|P-value:3.77E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-2.98|P-value:5.96E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.74|P-value:1.65E-7 ID:SGTC_544|Compound:k015-0020|FD-Score:-3.34|P-value:1.42E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-2.91|P-value:7.82E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:4.51|P-value:5.78E-7 ID:SGTC_788|Compound:1182-0090|FD-Score:3.28|P-value:2.09E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:3.10|P-value:4.18E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.13|P-value:3.40E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.20|P-value:3.06E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.21|P-value:2.72E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.54|P-value:6.87E-5 ID:SGTC_1590|Compound:st077108|FD-Score:3.10|P-value:4.17E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.06|P-value:4.89E-4 ID:SGTC_1688|Compound:st021091|FD-Score:4.30|P-value:1.82E-6 ID:SGTC_1712|Compound:st032288|FD-Score:8.86|P-value:5.46E-22 ID:SGTC_2002|Compound:4023503|FD-Score:3.84|P-value:1.76E-5 ID:SGTC_2006|Compound:4011487|FD-Score:3.08|P-value:4.51E-4 ID:SGTC_2021|Compound:4019055|FD-Score:7.20|P-value:3.97E-15 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.47|P-value:9.49E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.28|P-value:2.03E-4 ID:SGTC_2328|Compound:6201408|FD-Score:4.51|P-value:5.74E-7 ID:SGTC_2329|Compound:6138490|FD-Score:-5.10|P-value:1.63E-8 ID:SGTC_2330|Compound:7979373|FD-Score:-3.05|P-value:4.57E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.35|P-value:1.37E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.72|P-value:3.12E-5 ID:SGTC_2453|Compound:5731132|FD-Score:3.24|P-value:2.40E-4 ID:SGTC_2490|Compound:carminic acid|FD-Score:4.44|P-value:8.60E-7 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:-3.36|P-value:1.35E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.69|P-value:3.56E-5 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.52|P-value:6.79E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:3.44|P-value:1.06E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.26|P-value:2.25E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-2.86|P-value:9.64E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.69|P-value:3.13E-5 ID:SGTC_2849|Compound:9019321|FD-Score:6.04|P-value:3.66E-11 ID:SGTC_2856|Compound:9022925|FD-Score:3.12|P-value:3.94E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.33|P-value:1.66E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-4.99|P-value:3.23E-8 ID:SGTC_3037|Compound:9091026|FD-Score:3.28|P-value:2.10E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-3.09|P-value:4.00E-4 ID:SGTC_3075|Compound:9116269|FD-Score:3.30|P-value:1.88E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.50|P-value:7.23E-5 ID:SGTC_3201|Compound:9113999|FD-Score:3.00|P-value:6.10E-4 ID:SGTC_3308|Compound:9128332|FD-Score:3.16|P-value:3.38E-4 ID:SGTC_3334|Compound:9142812|FD-Score:-3.28|P-value:1.88E-4

Top fitness defect scores for YDR357C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1712 st032288 8.86 53.4 µM 5.46E-22 calcium & mitochondrial duress 34 3.88E-19
2 SGTC_2021 4019055 7.20 173.0 µM 3.97E-15 endomembrane recycling 28 3.03E-13
3 SGTC_2849 9019321 6.04 13.0 µM 3.66E-11 9 7.78E-10
4 SGTC_515 4092-1064 4.74 102.0 µM 1.65E-7 plasma membrane duress 42 1.09E-6
5 SGTC_398 bronopol 4.72 33.3 µM 1.81E-7 RNA processing & uracil transport 19 1.18E-6
6 SGTC_2328 6201408 4.51 200.0 µM 5.74E-7 12 3.20E-6
7 SGTC_617 3770-0098 4.51 35.0 µM 5.78E-7 exosome 9 3.22E-6
8 SGTC_2490 carminic acid 4.44 95.4 µM 8.60E-7 4 4.53E-6
9 SGTC_1688 st021091 4.30 57.6 µM 1.82E-6 4 8.65E-6
10 SGTC_1244 0416-0015 4.20 52.5 µM 3.06E-6 ERG2 29 1.36E-5
11 SGTC_2002 4023503 3.84 131.0 µM 1.76E-5 mitochondrial processes 36 6.16E-5
12 SGTC_2415 st077333 3.72 57.7 µM 3.12E-5 59 1.01E-4
13 SGTC_2537 larixol 3.69 68.9 µM 3.56E-5 28 1.13E-4
14 SGTC_308 4092-1169 3.68 104.0 µM 3.74E-5 plasma membrane duress 59 1.18E-4
15 SGTC_1288 0973-0013 3.54 65.5 µM 6.87E-5 26 2.00E-4
16 SGTC_2249 haloperidol 3.47 116.4 µM 9.49E-5 ergosterol depletion effects on membrane 148 2.65E-4
17 SGTC_2586 curcumin 3.44 80.0 µM 1.06E-4 iron homeostasis 79 2.92E-4
18 SGTC_351 caspofungin 3.40 25.0 nM 1.24E-4 cell wall 144 3.34E-4
19 SGTC_2857 9024691 3.33 58.4 µM 1.66E-4 43 4.29E-4
20 SGTC_3075 9116269 3.30 49.5 µM 1.88E-4 40 4.80E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.249 1.47E-48 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.247 9.43E-48 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.228 8.50E-41 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.225 1.17E-39 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.224 2.32E-39 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.195 4.04E-30 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.193 1.41E-29 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.189 2.85E-28 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.187 9.43E-28 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.181 5.29E-26 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.178 3.13E-25 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.177 4.23E-25 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.177 5.66E-25 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.175 1.35E-24 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.171 2.13E-23 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes