YDR363W-A / SEM1

Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR363W-A / SEM1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.20|P-value:6.82E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.31|P-value:5.37E-6 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.16|P-value:6.05E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:4.63|P-value:1.67E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:4.10|P-value:2.02E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.42|P-value:2.39E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.36|P-value:2.98E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.36|P-value:2.91E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.68|P-value:1.15E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.41|P-value:2.42E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.63|P-value:1.67E-6 ID:SGTC_559|Compound:1550-0661|FD-Score:-3.82|P-value:4.69E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.20|P-value:5.21E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.23|P-value:6.25E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:3.42|P-value:3.09E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.58|P-value:1.71E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:4.40|P-value:5.12E-6 ID:SGTC_856|Compound:0433-0016|FD-Score:5.39|P-value:3.06E-8 ID:SGTC_896|Compound:0964-0007|FD-Score:-3.03|P-value:9.47E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.59|P-value:1.66E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.43|P-value:3.04E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-4.72|P-value:7.59E-7 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.63|P-value:1.39E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:4.12|P-value:1.81E-5 ID:SGTC_1637|Compound:st007924|FD-Score:5.15|P-value:1.17E-7 ID:SGTC_1724|Compound:st037281|FD-Score:3.67|P-value:1.21E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.53|P-value:2.76E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.13|P-value:8.89E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.37|P-value:2.79E-4 ID:SGTC_1862|Compound:st057513|FD-Score:4.16|P-value:1.52E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.87|P-value:5.37E-5 ID:SGTC_2074|Compound:5212007|FD-Score:-3.05|P-value:8.90E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-3.13|P-value:6.76E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.30|P-value:4.85E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.17|P-value:1.50E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.37|P-value:3.70E-4 ID:SGTC_2285|Compound:7945142|FD-Score:-5.19|P-value:6.12E-8 ID:SGTC_2289|Compound:7946404|FD-Score:5.25|P-value:6.93E-8 ID:SGTC_2310|Compound:7668677|FD-Score:3.31|P-value:4.72E-4 ID:SGTC_2318|Compound:6029776|FD-Score:-3.07|P-value:8.36E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.12|P-value:9.03E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.10|P-value:9.78E-4 ID:SGTC_2462|Compound:5162487|FD-Score:-3.28|P-value:3.94E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.22|P-value:6.44E-4 ID:SGTC_2671|Compound:danthron|FD-Score:-3.14|P-value:6.52E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.61|P-value:1.85E-6 ID:SGTC_2880|Compound:9042983|FD-Score:-3.35|P-value:3.09E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.85|P-value:5.86E-5 ID:SGTC_2906|Compound:7926292|FD-Score:4.55|P-value:2.47E-6 ID:SGTC_3089|Compound:9117425|FD-Score:-3.13|P-value:6.77E-4 ID:SGTC_3099|Compound:9120367|FD-Score:3.65|P-value:1.27E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.84|P-value:6.12E-5 ID:SGTC_3214|Compound:9129694|FD-Score:-3.30|P-value:3.71E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.55|P-value:1.41E-4 ID:SGTC_3271|Compound:9137798|FD-Score:-3.85|P-value:4.24E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.20|P-value:6.82E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-4.31|P-value:5.37E-6 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.16|P-value:6.05E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:4.63|P-value:1.67E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:4.10|P-value:2.02E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.42|P-value:2.39E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.36|P-value:2.98E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.36|P-value:2.91E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.68|P-value:1.15E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.41|P-value:2.42E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.63|P-value:1.67E-6 ID:SGTC_559|Compound:1550-0661|FD-Score:-3.82|P-value:4.69E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.20|P-value:5.21E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.23|P-value:6.25E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:3.42|P-value:3.09E-4 ID:SGTC_826|Compound:1683-5028|FD-Score:3.58|P-value:1.71E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:4.40|P-value:5.12E-6 ID:SGTC_856|Compound:0433-0016|FD-Score:5.39|P-value:3.06E-8 ID:SGTC_896|Compound:0964-0007|FD-Score:-3.03|P-value:9.47E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.59|P-value:1.66E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.43|P-value:3.04E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-4.72|P-value:7.59E-7 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.63|P-value:1.39E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:4.12|P-value:1.81E-5 ID:SGTC_1637|Compound:st007924|FD-Score:5.15|P-value:1.17E-7 ID:SGTC_1724|Compound:st037281|FD-Score:3.67|P-value:1.21E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.53|P-value:2.76E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.13|P-value:8.89E-4 ID:SGTC_1856|Compound:st057054|FD-Score:-3.37|P-value:2.79E-4 ID:SGTC_1862|Compound:st057513|FD-Score:4.16|P-value:1.52E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.87|P-value:5.37E-5 ID:SGTC_2074|Compound:5212007|FD-Score:-3.05|P-value:8.90E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-3.13|P-value:6.76E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.30|P-value:4.85E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.17|P-value:1.50E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.37|P-value:3.70E-4 ID:SGTC_2285|Compound:7945142|FD-Score:-5.19|P-value:6.12E-8 ID:SGTC_2289|Compound:7946404|FD-Score:5.25|P-value:6.93E-8 ID:SGTC_2310|Compound:7668677|FD-Score:3.31|P-value:4.72E-4 ID:SGTC_2318|Compound:6029776|FD-Score:-3.07|P-value:8.36E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.12|P-value:9.03E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.10|P-value:9.78E-4 ID:SGTC_2462|Compound:5162487|FD-Score:-3.28|P-value:3.94E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.22|P-value:6.44E-4 ID:SGTC_2671|Compound:danthron|FD-Score:-3.14|P-value:6.52E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.61|P-value:1.85E-6 ID:SGTC_2880|Compound:9042983|FD-Score:-3.35|P-value:3.09E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.85|P-value:5.86E-5 ID:SGTC_2906|Compound:7926292|FD-Score:4.55|P-value:2.47E-6 ID:SGTC_3089|Compound:9117425|FD-Score:-3.13|P-value:6.77E-4 ID:SGTC_3099|Compound:9120367|FD-Score:3.65|P-value:1.27E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.84|P-value:6.12E-5 ID:SGTC_3214|Compound:9129694|FD-Score:-3.30|P-value:3.71E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.55|P-value:1.41E-4 ID:SGTC_3271|Compound:9137798|FD-Score:-3.85|P-value:4.24E-5

Top fitness defect scores for YDR363W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_856 0433-0016 5.39 11.3 µM 3.06E-8 4 3.47E-8
2 SGTC_2289 7946404 5.25 165.1 µM 6.93E-8 49 7.78E-8
3 SGTC_1637 st007924 5.15 17.4 µM 1.17E-7 9 1.30E-7
4 SGTC_176 1486-1328 4.63 72.8 µM 1.67E-6 39 1.79E-6
5 SGTC_543 3844-1095 4.63 191.0 µM 1.67E-6 ERG2 36 1.79E-6
6 SGTC_2679 rapamycin 4.61 1.0 nM 1.85E-6 111 1.97E-6
7 SGTC_2906 7926292 4.55 26.4 µM 2.47E-6 14 2.64E-6
8 SGTC_1768 st044301 4.53 37.2 µM 2.76E-6 8 2.94E-6
9 SGTC_838 0109-0203 4.40 71.4 µM 5.12E-6 9 5.40E-6
10 SGTC_2181 6316240 4.17 54.3 µM 1.50E-5 17 1.56E-5
11 SGTC_1862 st057513 4.16 3.8 µM 1.52E-5 8 1.59E-5
12 SGTC_1387 3342-1166 4.12 1.8 µM 1.81E-5 mitochondrial processes 11 1.88E-5
13 SGTC_178 4092-0806 4.10 87.5 µM 2.02E-5 ergosterol depletion effects on membrane 88 2.09E-5
14 SGTC_1873 rapamycin 3.87 2.0 nM 5.37E-5 71 5.50E-5
15 SGTC_2896 9052656 3.85 71.4 µM 5.86E-5 endomembrane recycling 121 5.99E-5
16 SGTC_3208 9114115 3.84 49.5 µM 6.12E-5 41 6.25E-5
17 SGTC_402 epigallocatechin gallate 3.68 10.0 µM 1.15E-4 36 1.16E-4
18 SGTC_1724 st037281 3.67 8.3 µM 1.21E-4 52 1.22E-4
19 SGTC_3099 9120367 3.65 49.5 µM 1.27E-4 22 1.29E-4
20 SGTC_1365 1672-3266 3.63 55.4 µM 1.39E-4 31 1.41E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.193 1.20E-29 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.181 4.55E-26 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.156 9.31E-20 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.152 1.01E-18 YBR111W-A SUS1 hom Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
0.144 4.85E-17 YFL001W DEG1 hom tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
0.130 3.78E-14 YBR077C SLM4 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.127 1.49E-13 YDL041W_d YDL041W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
0.126 2.54E-13 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.125 3.86E-13 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.124 4.92E-13 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.123 8.51E-13 YLR342W FKS1 hom Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.121 2.36E-12 YGR229C SMI1 hom Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
0.119 4.50E-12 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.114 3.48E-11 YPR159W KRE6 hom Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p
0.112 9.40E-11 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB