YDR365C / ESF1

Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR365C / ESF1

Click on Significant Values for Screen Details ID:SGTC_161|Compound:4065-0411|FD-Score:2.60|P-value:4.99E-4 ID:SGTC_271|Compound:rotenone|FD-Score:2.63|P-value:4.39E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-2.63|P-value:4.25E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.70|P-value:3.07E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:2.50|P-value:7.85E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-2.46|P-value:9.10E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:2.77|P-value:2.35E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:2.45|P-value:9.67E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:2.92|P-value:1.14E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.11|P-value:4.30E-5 ID:SGTC_1269|Compound:0831-0796|FD-Score:2.68|P-value:3.55E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.21|P-value:2.52E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:-2.92|P-value:1.04E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:2.78|P-value:2.17E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.21|P-value:2.40E-5 ID:SGTC_1667|Compound:st014849|FD-Score:-3.19|P-value:2.63E-5 ID:SGTC_1670|Compound:st016595|FD-Score:-2.84|P-value:1.61E-4 ID:SGTC_1706|Compound:st031321|FD-Score:2.76|P-value:2.43E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-2.76|P-value:2.33E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-2.56|P-value:5.83E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.28|P-value:1.08E-11 ID:SGTC_2172|Compound:5754775|FD-Score:-3.92|P-value:3.41E-7 ID:SGTC_2178|Compound:5812956|FD-Score:-4.30|P-value:2.45E-8 ID:SGTC_2182|Compound:6325192|FD-Score:2.49|P-value:8.30E-4 ID:SGTC_2237|Compound:6667747|FD-Score:2.70|P-value:3.24E-4 ID:SGTC_2238|Compound:6671620|FD-Score:4.09|P-value:1.11E-7 ID:SGTC_2246|Compound:7293507|FD-Score:3.19|P-value:2.68E-5 ID:SGTC_2247|Compound:cerulenin|FD-Score:-4.97|P-value:1.54E-10 ID:SGTC_2350|Compound:9023895|FD-Score:3.10|P-value:4.50E-5 ID:SGTC_2444|Compound:5485484|FD-Score:3.67|P-value:1.71E-6 ID:SGTC_2451|Compound:5856223|FD-Score:-2.64|P-value:4.04E-4 ID:SGTC_2456|Compound:5770359|FD-Score:3.40|P-value:8.69E-6 ID:SGTC_2462|Compound:5162487|FD-Score:3.06|P-value:5.38E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.95|P-value:2.84E-7 ID:SGTC_2821|Compound:7996764|FD-Score:-3.19|P-value:2.61E-5 ID:SGTC_2932|Compound:9016711|FD-Score:2.69|P-value:3.28E-4 ID:SGTC_2960|Compound:9079389|FD-Score:-2.55|P-value:6.19E-4 ID:SGTC_2991|Compound:9074669|FD-Score:2.65|P-value:4.04E-4 ID:SGTC_3081|Compound:9117243|FD-Score:2.78|P-value:2.20E-4 ID:SGTC_3084|Compound:9116877|FD-Score:2.96|P-value:8.87E-5 ID:SGTC_3197|Compound:9113331|FD-Score:-3.11|P-value:4.04E-5 ID:SGTC_3254|Compound:9136651|FD-Score:-2.55|P-value:6.12E-4 ID:SGTC_3295|Compound:9122362|FD-Score:-2.50|P-value:7.45E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.60|P-value:4.99E-4 ID:SGTC_271|Compound:rotenone|FD-Score:2.63|P-value:4.39E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-2.63|P-value:4.25E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.70|P-value:3.07E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:2.50|P-value:7.85E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-2.46|P-value:9.10E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:2.77|P-value:2.35E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:2.45|P-value:9.67E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:2.92|P-value:1.14E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.11|P-value:4.30E-5 ID:SGTC_1269|Compound:0831-0796|FD-Score:2.68|P-value:3.55E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.21|P-value:2.52E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:-2.92|P-value:1.04E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:2.78|P-value:2.17E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.21|P-value:2.40E-5 ID:SGTC_1667|Compound:st014849|FD-Score:-3.19|P-value:2.63E-5 ID:SGTC_1670|Compound:st016595|FD-Score:-2.84|P-value:1.61E-4 ID:SGTC_1706|Compound:st031321|FD-Score:2.76|P-value:2.43E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-2.76|P-value:2.33E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-2.56|P-value:5.83E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.28|P-value:1.08E-11 ID:SGTC_2172|Compound:5754775|FD-Score:-3.92|P-value:3.41E-7 ID:SGTC_2178|Compound:5812956|FD-Score:-4.30|P-value:2.45E-8 ID:SGTC_2182|Compound:6325192|FD-Score:2.49|P-value:8.30E-4 ID:SGTC_2237|Compound:6667747|FD-Score:2.70|P-value:3.24E-4 ID:SGTC_2238|Compound:6671620|FD-Score:4.09|P-value:1.11E-7 ID:SGTC_2246|Compound:7293507|FD-Score:3.19|P-value:2.68E-5 ID:SGTC_2247|Compound:cerulenin|FD-Score:-4.97|P-value:1.54E-10 ID:SGTC_2350|Compound:9023895|FD-Score:3.10|P-value:4.50E-5 ID:SGTC_2444|Compound:5485484|FD-Score:3.67|P-value:1.71E-6 ID:SGTC_2451|Compound:5856223|FD-Score:-2.64|P-value:4.04E-4 ID:SGTC_2456|Compound:5770359|FD-Score:3.40|P-value:8.69E-6 ID:SGTC_2462|Compound:5162487|FD-Score:3.06|P-value:5.38E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.95|P-value:2.84E-7 ID:SGTC_2821|Compound:7996764|FD-Score:-3.19|P-value:2.61E-5 ID:SGTC_2932|Compound:9016711|FD-Score:2.69|P-value:3.28E-4 ID:SGTC_2960|Compound:9079389|FD-Score:-2.55|P-value:6.19E-4 ID:SGTC_2991|Compound:9074669|FD-Score:2.65|P-value:4.04E-4 ID:SGTC_3081|Compound:9117243|FD-Score:2.78|P-value:2.20E-4 ID:SGTC_3084|Compound:9116877|FD-Score:2.96|P-value:8.87E-5 ID:SGTC_3197|Compound:9113331|FD-Score:-3.11|P-value:4.04E-5 ID:SGTC_3254|Compound:9136651|FD-Score:-2.55|P-value:6.12E-4 ID:SGTC_3295|Compound:9122362|FD-Score:-2.50|P-value:7.45E-4

Top fitness defect scores for YDR365C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2238 6671620 4.09 73.2 µM 1.11E-7 4 2.11E-5 0.18
2 SGTC_2600 tangeritin 3.95 88.0 µM 2.84E-7 4 3.85E-5 0.44
3 SGTC_2444 5485484 3.67 36.4 µM 1.71E-6 11 1.21E-4 0.02
4 SGTC_2456 5770359 3.40 74.5 µM 8.69E-6 7 3.41E-4 0.00
5 SGTC_1645 Cervolide 3.21 78.0 µM 2.40E-5 9 6.54E-4 0.14
6 SGTC_1310 1188-0297 3.21 994.0 nM 2.52E-5 azole & statin 23 6.75E-4 0.06
7 SGTC_2246 7293507 3.19 91.6 µM 2.68E-5 23 7.01E-4 0.07
8 SGTC_1177 0983-0204 3.11 23.6 µM 4.30E-5 22 9.51E-4 0.12
9 SGTC_2350 9023895 3.10 38.9 µM 4.50E-5 ergosterol biosynthesis 14 9.79E-4 0.08
10 SGTC_2462 5162487 3.06 171.8 µM 5.38E-5 12 0.00110 0.04
11 SGTC_3084 9116877 2.96 49.5 µM 8.87E-5 4 0.00152 0.25
12 SGTC_1166 k206-0078 2.92 225.0 µM 1.14E-4 12 0.00178 0.01
13 SGTC_1402 3909-7673 2.78 35.9 µM 2.17E-4 15 0.00270 0.06
14 SGTC_3081 9117243 2.78 49.5 µM 2.20E-4 fatty acid desaturase (OLE1) 61 0.00272 0.01
15 SGTC_1056 3448-1962 2.77 115.0 µM 2.35E-4 amide catabolism 19 0.00284 0.00
16 SGTC_1706 st031321 2.76 10.0 µM 2.43E-4 TSC3-RPN4 12 0.00290 0.02
17 SGTC_2237 6667747 2.70 47.2 µM 3.24E-4 14 0.00350 0.04
18 SGTC_2932 9016711 2.69 44.5 µM 3.28E-4 26 0.00352 0.00
19 SGTC_1269 0831-0796 2.68 3.0 µM 3.55E-4 60S ribosome export 24 0.00371 0.06
20 SGTC_2991 9074669 2.65 71.4 µM 4.04E-4 azole & statin 55 0.00403 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.130 3.90E-14 YDR397C NCB2 het Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
0.120 2.77E-12 YDR355C_d YDR355C_d het Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
0.120 3.46E-12 YDR037W KRS1 het Lysyl-tRNA synthetase
0.119 5.62E-12 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.113 5.43E-11 YDR390C UBA2 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.104 1.62E-9 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.102 3.90E-9 YIL161W_p YIL161W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene
0.101 4.45E-9 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.100 5.99E-9 YFR042W KEG1 het Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
0.098 1.21E-8 YBR087W RFC5 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.098 1.26E-8 YIL046W MET30 het F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
0.096 2.66E-8 YDR434W GPI17 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
0.091 1.35E-7 YKR063C LAS1 het Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
0.088 2.91E-7 YPR155C NCA2 hom Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
0.086 5.75E-7 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency