YDR385W / EFT2

Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR385W / EFT2

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.10|P-value:5.91E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:5.29|P-value:1.55E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.65|P-value:5.55E-7 ID:SGTC_246|Compound:cycloheximide|FD-Score:5.92|P-value:2.83E-10 ID:SGTC_253|Compound:dyclonine|FD-Score:3.54|P-value:1.05E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.50|P-value:1.26E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:4.38|P-value:2.31E-6 ID:SGTC_377|Compound:3970-0964|FD-Score:4.85|P-value:1.87E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.45|P-value:1.49E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.21|P-value:5.12E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:5.96|P-value:2.18E-10 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.34|P-value:2.29E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.09|P-value:5.92E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:-3.09|P-value:6.02E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:4.02|P-value:1.30E-5 ID:SGTC_743|Compound:1275-0778|FD-Score:2.99|P-value:8.83E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:3.49|P-value:1.30E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:4.02|P-value:1.27E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:3.68|P-value:5.89E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:3.56|P-value:9.51E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.25|P-value:3.33E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.80|P-value:3.45E-5 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.32|P-value:2.59E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.20|P-value:3.99E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:3.90|P-value:2.20E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:-3.65|P-value:6.42E-5 ID:SGTC_1398|Compound:3558-0053|FD-Score:3.47|P-value:1.38E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.59|P-value:8.48E-5 ID:SGTC_1689|Compound:st020265|FD-Score:-3.32|P-value:2.54E-4 ID:SGTC_1701|Compound:st028638|FD-Score:3.02|P-value:7.88E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.41|P-value:1.81E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.78|P-value:2.77E-7 ID:SGTC_1948|Compound:st076329|FD-Score:3.52|P-value:1.12E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.09|P-value:6.05E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.18|P-value:4.29E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.32|P-value:2.48E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.16|P-value:4.76E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.41|P-value:1.77E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.11|P-value:5.70E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.69|P-value:5.59E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.77|P-value:6.49E-13 ID:SGTC_2701|Compound:st077767|FD-Score:3.34|P-value:2.38E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.53|P-value:1.09E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.00|P-value:8.51E-4 ID:SGTC_2858|Compound:9025781|FD-Score:4.94|P-value:1.15E-7 ID:SGTC_2874|Compound:9043904|FD-Score:-3.78|P-value:3.76E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-3.22|P-value:3.65E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.04|P-value:7.37E-4 ID:SGTC_2943|Compound:9068529|FD-Score:-3.60|P-value:8.20E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-3.02|P-value:7.85E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.70|P-value:5.45E-5 ID:SGTC_2985|Compound:9022375|FD-Score:3.24|P-value:3.45E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-3.66|P-value:6.17E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.36|P-value:2.13E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.51|P-value:1.18E-4 ID:SGTC_3271|Compound:9137798|FD-Score:3.40|P-value:1.83E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.10|P-value:5.91E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:5.29|P-value:1.55E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.65|P-value:5.55E-7 ID:SGTC_246|Compound:cycloheximide|FD-Score:5.92|P-value:2.83E-10 ID:SGTC_253|Compound:dyclonine|FD-Score:3.54|P-value:1.05E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.50|P-value:1.26E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:4.38|P-value:2.31E-6 ID:SGTC_377|Compound:3970-0964|FD-Score:4.85|P-value:1.87E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.45|P-value:1.49E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.21|P-value:5.12E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:5.96|P-value:2.18E-10 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.34|P-value:2.29E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.09|P-value:5.92E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:-3.09|P-value:6.02E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:4.02|P-value:1.30E-5 ID:SGTC_743|Compound:1275-0778|FD-Score:2.99|P-value:8.83E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:3.49|P-value:1.30E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:4.02|P-value:1.27E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:3.68|P-value:5.89E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:3.56|P-value:9.51E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.25|P-value:3.33E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.80|P-value:3.45E-5 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.32|P-value:2.59E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.20|P-value:3.99E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:3.90|P-value:2.20E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:-3.65|P-value:6.42E-5 ID:SGTC_1398|Compound:3558-0053|FD-Score:3.47|P-value:1.38E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.59|P-value:8.48E-5 ID:SGTC_1689|Compound:st020265|FD-Score:-3.32|P-value:2.54E-4 ID:SGTC_1701|Compound:st028638|FD-Score:3.02|P-value:7.88E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.41|P-value:1.81E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.78|P-value:2.77E-7 ID:SGTC_1948|Compound:st076329|FD-Score:3.52|P-value:1.12E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.09|P-value:6.05E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.18|P-value:4.29E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.32|P-value:2.48E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.16|P-value:4.76E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.41|P-value:1.77E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.11|P-value:5.70E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.69|P-value:5.59E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.77|P-value:6.49E-13 ID:SGTC_2701|Compound:st077767|FD-Score:3.34|P-value:2.38E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.53|P-value:1.09E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.00|P-value:8.51E-4 ID:SGTC_2858|Compound:9025781|FD-Score:4.94|P-value:1.15E-7 ID:SGTC_2874|Compound:9043904|FD-Score:-3.78|P-value:3.76E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-3.22|P-value:3.65E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.04|P-value:7.37E-4 ID:SGTC_2943|Compound:9068529|FD-Score:-3.60|P-value:8.20E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-3.02|P-value:7.85E-4 ID:SGTC_2982|Compound:9019404|FD-Score:3.70|P-value:5.45E-5 ID:SGTC_2985|Compound:9022375|FD-Score:3.24|P-value:3.45E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-3.66|P-value:6.17E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.36|P-value:2.13E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.51|P-value:1.18E-4 ID:SGTC_3271|Compound:9137798|FD-Score:3.40|P-value:1.83E-4

Top fitness defect scores for YDR385W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2651 1327-0036 6.77 5.0 µM 6.49E-13 51 6.24E-12
2 SGTC_513 4245-1575 5.96 41.7 µM 2.18E-10 unfolded protein response 47 1.26E-9
3 SGTC_246 cycloheximide 5.92 667.0 µM 2.83E-10 4 1.60E-9
4 SGTC_212 3807-4682 5.29 38.8 µM 1.55E-8 tubulin folding & SWR complex 35 6.22E-8
5 SGTC_2858 9025781 4.94 9.0 µM 1.15E-7 sphingolipid biosynthesis & PDR1 69 3.89E-7
6 SGTC_377 3970-0964 4.85 33.1 µM 1.87E-7 28 6.07E-7
7 SGTC_1941 st076221 4.78 61.8 µM 2.77E-7 sphingolipid biosynthesis & PDR1 53 8.70E-7
8 SGTC_234 nsc-17383 4.65 10.7 µM 5.55E-7 ERG2 15 1.64E-6
9 SGTC_352 k048-0007 4.38 5.1 µM 2.31E-6 ERAD & cell cycle 66 6.05E-6
10 SGTC_488 nitrendipine 4.21 139.0 µM 5.12E-6 38 1.25E-5
11 SGTC_904 1141-0088 4.02 396.0 µM 1.27E-5 12 2.87E-5
12 SGTC_740 k014-0057 4.02 261.0 µM 1.30E-5 24 2.95E-5
13 SGTC_1296 1068-0102 3.90 11.9 µM 2.20E-5 9 4.77E-5
14 SGTC_1178 0988-0033 3.80 191.0 µM 3.45E-5 31 7.20E-5
15 SGTC_2982 9019404 3.70 26.0 µM 5.45E-5 24 1.09E-4
16 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 3.69 100.0 µM 5.59E-5 43 1.12E-4
17 SGTC_922 1866-0035 3.68 480.0 µM 5.89E-5 excess fatty acid 72 1.17E-4
18 SGTC_1469 k284-2957 3.59 126.0 µM 8.48E-5 16 1.64E-4
19 SGTC_994 3910-0338 3.56 138.0 µM 9.51E-5 calcium & mitochondrial duress 62 1.82E-4
20 SGTC_253 dyclonine 3.54 31.2 µM 1.05E-4 amide catabolism 41 2.00E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.201 7.16E-32 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.188 4.74E-28 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.172 1.27E-23 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.168 1.63E-22 YMR031W-A_d YMR031W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C
0.162 4.42E-21 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.157 5.24E-20 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.155 1.40E-19 YDR403W DIT1 hom Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
0.149 3.17E-18 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.139 7.26E-16 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.139 7.83E-16 YNL235C_d YNL235C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
0.137 1.34E-15 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.137 1.36E-15 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.137 1.55E-15 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.134 6.06E-15 YPL138C SPP1 hom Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein
0.132 1.77E-14 YGR164W_d YGR164W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data