YDR386W / MUS81

Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR386W / MUS81

Click on Significant Values for Screen Details ID:SGTC_244|Compound:nsc-207895|FD-Score:15.20|P-value:2.65E-45 ID:SGTC_248|Compound:streptozotocin|FD-Score:10.80|P-value:6.52E-24 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.30|P-value:3.30E-40 ID:SGTC_268|Compound:camptothecin|FD-Score:11.00|P-value:1.31E-24 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.50|P-value:3.76E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:4.27|P-value:5.29E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:4.30|P-value:4.67E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.91|P-value:1.97E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.93|P-value:3.48E-6 ID:SGTC_829|Compound:1635-0120|FD-Score:3.84|P-value:2.48E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:4.71|P-value:8.88E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.22|P-value:9.39E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.62|P-value:5.37E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:5.54|P-value:2.00E-7 ID:SGTC_996|Compound:3970-0799|FD-Score:3.52|P-value:7.41E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.27|P-value:5.28E-5 ID:SGTC_1079|Compound:idarubicin|FD-Score:5.38|P-value:4.35E-7 ID:SGTC_1082|Compound:camptothecin|FD-Score:21.00|P-value:3.42E-85 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.93|P-value:1.84E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.76|P-value:3.35E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:-4.13|P-value:3.77E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.82|P-value:2.73E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:5.08|P-value:1.74E-6 ID:SGTC_1554|Compound:st069299|FD-Score:4.95|P-value:3.15E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.84|P-value:2.52E-4 ID:SGTC_1630|Compound:st005209|FD-Score:5.42|P-value:3.69E-7 ID:SGTC_1707|Compound:st032323|FD-Score:16.20|P-value:1.57E-51 ID:SGTC_1718|Compound:roemerine|FD-Score:4.49|P-value:2.21E-5 ID:SGTC_1845|Compound:st056753|FD-Score:8.65|P-value:8.19E-16 ID:SGTC_1906|Compound:st060206|FD-Score:4.91|P-value:3.71E-6 ID:SGTC_1913|Compound:st060213|FD-Score:4.45|P-value:2.59E-5 ID:SGTC_1960|Compound:st011366|FD-Score:-3.24|P-value:8.72E-4 ID:SGTC_1970|Compound:st070122|FD-Score:4.10|P-value:9.99E-5 ID:SGTC_2386|Compound:9075165|FD-Score:4.46|P-value:2.46E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.85|P-value:2.43E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:6.03|P-value:1.63E-8 ID:SGTC_2671|Compound:danthron|FD-Score:6.82|P-value:1.91E-10 ID:SGTC_2693|Compound:st077581|FD-Score:5.09|P-value:1.68E-6 ID:SGTC_2736|Compound:butenafine|FD-Score:4.23|P-value:6.05E-5 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.80|P-value:2.88E-4 ID:SGTC_2746|Compound:daunorubicin|FD-Score:3.74|P-value:3.58E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.85|P-value:1.10E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.43|P-value:9.66E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-3.21|P-value:9.58E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:15.20|P-value:2.65E-45 ID:SGTC_248|Compound:streptozotocin|FD-Score:10.80|P-value:6.52E-24 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.30|P-value:3.30E-40 ID:SGTC_268|Compound:camptothecin|FD-Score:11.00|P-value:1.31E-24 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.50|P-value:3.76E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:4.27|P-value:5.29E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:4.30|P-value:4.67E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.91|P-value:1.97E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.93|P-value:3.48E-6 ID:SGTC_829|Compound:1635-0120|FD-Score:3.84|P-value:2.48E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:4.71|P-value:8.88E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.22|P-value:9.39E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.62|P-value:5.37E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:5.54|P-value:2.00E-7 ID:SGTC_996|Compound:3970-0799|FD-Score:3.52|P-value:7.41E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.27|P-value:5.28E-5 ID:SGTC_1079|Compound:idarubicin|FD-Score:5.38|P-value:4.35E-7 ID:SGTC_1082|Compound:camptothecin|FD-Score:21.00|P-value:3.42E-85 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.93|P-value:1.84E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.76|P-value:3.35E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:-4.13|P-value:3.77E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.82|P-value:2.73E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:5.08|P-value:1.74E-6 ID:SGTC_1554|Compound:st069299|FD-Score:4.95|P-value:3.15E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.84|P-value:2.52E-4 ID:SGTC_1630|Compound:st005209|FD-Score:5.42|P-value:3.69E-7 ID:SGTC_1707|Compound:st032323|FD-Score:16.20|P-value:1.57E-51 ID:SGTC_1718|Compound:roemerine|FD-Score:4.49|P-value:2.21E-5 ID:SGTC_1845|Compound:st056753|FD-Score:8.65|P-value:8.19E-16 ID:SGTC_1906|Compound:st060206|FD-Score:4.91|P-value:3.71E-6 ID:SGTC_1913|Compound:st060213|FD-Score:4.45|P-value:2.59E-5 ID:SGTC_1960|Compound:st011366|FD-Score:-3.24|P-value:8.72E-4 ID:SGTC_1970|Compound:st070122|FD-Score:4.10|P-value:9.99E-5 ID:SGTC_2386|Compound:9075165|FD-Score:4.46|P-value:2.46E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.85|P-value:2.43E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:6.03|P-value:1.63E-8 ID:SGTC_2671|Compound:danthron|FD-Score:6.82|P-value:1.91E-10 ID:SGTC_2693|Compound:st077581|FD-Score:5.09|P-value:1.68E-6 ID:SGTC_2736|Compound:butenafine|FD-Score:4.23|P-value:6.05E-5 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.80|P-value:2.88E-4 ID:SGTC_2746|Compound:daunorubicin|FD-Score:3.74|P-value:3.58E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.85|P-value:1.10E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.43|P-value:9.66E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-3.21|P-value:9.58E-4

Top fitness defect scores for YDR386W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1082 camptothecin 21.00 424.9 nM 3.42E-85 DNA damage response 2 1.72E-98
2 SGTC_1707 st032323 16.20 6.3 µM 1.57E-51 DNA damage response 2 1.24E-59
3 SGTC_244 nsc-207895 15.20 1.3 µM 2.65E-45 DNA damage response 1 1.94E-52
4 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 14.30 44.4 nM 3.30E-40 DNA damage response 9 1.51E-46
5 SGTC_268 camptothecin 11.00 39.6 µM 1.31E-24 DNA damage response 7 1.69E-28
6 SGTC_248 streptozotocin 10.80 5.2 mM 6.52E-24 DNA damage response 1 1.08E-27
7 SGTC_1845 st056753 8.65 3.0 µM 8.19E-16 TSC3-RPN4 3 2.65E-18
8 SGTC_2671 danthron 6.82 100.0 µM 1.91E-10 DNA damage response 8 4.54E-12
9 SGTC_2612 dictamnine 6.03 15.5 µM 1.63E-8 RNA pol III & RNase P/MRP 11 8.05E-10
10 SGTC_955 1216-0110 5.54 33.0 µM 2.00E-7 sphingolipid biosynthesis & PDR1 39 1.49E-8
11 SGTC_1630 st005209 5.42 31.3 µM 3.69E-7 7 3.03E-8
12 SGTC_1079 idarubicin 5.38 2.4 µM 4.35E-7 DNA damage response 9 3.68E-8
13 SGTC_2693 st077581 5.09 40.7 µM 1.68E-6 12 1.78E-7
14 SGTC_1357 1503-1303 5.08 39.5 µM 1.74E-6 12 1.86E-7
15 SGTC_1554 st069299 4.95 38.7 µM 3.15E-6 5 3.70E-7
16 SGTC_828 2074-0507 4.93 139.0 µM 3.48E-6 RPP1 & pyrimidine depletion 9 4.16E-7
17 SGTC_1906 st060206 4.91 94.7 µM 3.71E-6 10 4.48E-7
18 SGTC_867 0518-0118 4.71 155.0 µM 8.88E-6 DNA intercalators 11 1.24E-6
19 SGTC_1718 roemerine 4.49 11.9 µM 2.21E-5 redox potentiating 33 3.60E-6
20 SGTC_2386 9075165 4.46 157.4 µM 2.46E-5 4 4.08E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.666 0 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.619 0 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.604 0 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.453 1.75E-169 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.423 7.24E-146 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.422 8.15E-145 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.409 2.88E-135 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.353 3.98E-99 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.313 4.54E-77 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.285 7.53E-64 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.257 1.28E-51 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.253 2.74E-50 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.252 7.51E-50 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.236 9.63E-44 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.215 1.46E-36 YPR066W UBA3 hom Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern