YDR394W / RPT3

One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR394W / RPT3

Click on Significant Values for Screen Details ID:SGTC_104|Compound:0074-0074|FD-Score:3.92|P-value:3.45E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.79|P-value:5.21E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:4.93|P-value:1.05E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.25|P-value:1.21E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.79|P-value:5.24E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.49|P-value:5.28E-5 ID:SGTC_273|Compound:hydroxyurea|FD-Score:5.41|P-value:1.53E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:7.01|P-value:8.15E-10 ID:SGTC_547|Compound:1120-0019|FD-Score:-5.29|P-value:3.53E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:6.56|P-value:8.03E-9 ID:SGTC_553|Compound:k007-0256|FD-Score:11.60|P-value:1.59E-23 ID:SGTC_676|Compound:falcarinol|FD-Score:3.81|P-value:4.97E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.52|P-value:9.77E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.71|P-value:6.67E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:4.96|P-value:9.40E-6 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.66|P-value:7.60E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.90|P-value:3.75E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.62|P-value:8.64E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-4.17|P-value:2.07E-4 ID:SGTC_1723|Compound:st034307|FD-Score:6.28|P-value:3.20E-8 ID:SGTC_1749|Compound:st037932|FD-Score:4.81|P-value:1.64E-5 ID:SGTC_1765|Compound:st044815|FD-Score:4.09|P-value:2.02E-4 ID:SGTC_1775|Compound:st081588|FD-Score:5.54|P-value:8.82E-7 ID:SGTC_1812|Compound:st052057|FD-Score:-4.08|P-value:2.79E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.95|P-value:3.20E-4 ID:SGTC_2044|Compound:5102272|FD-Score:4.38|P-value:7.69E-5 ID:SGTC_2124|Compound:5246320|FD-Score:13.90|P-value:2.93E-33 ID:SGTC_2168|Compound:5727979|FD-Score:4.32|P-value:9.56E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.76|P-value:2.01E-5 ID:SGTC_2231|Compound:micatex|FD-Score:3.77|P-value:5.49E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.79|P-value:5.15E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.18|P-value:1.53E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.30|P-value:1.00E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:9.67|P-value:4.82E-17 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:5.53|P-value:9.52E-7 ID:SGTC_2938|Compound:9059746|FD-Score:3.61|P-value:8.94E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.67|P-value:9.47E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-3.72|P-value:8.37E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-5.20|P-value:5.07E-6 ID:SGTC_104|Compound:0074-0074|FD-Score:3.92|P-value:3.45E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.79|P-value:5.21E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:4.93|P-value:1.05E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.25|P-value:1.21E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.79|P-value:5.24E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.49|P-value:5.28E-5 ID:SGTC_273|Compound:hydroxyurea|FD-Score:5.41|P-value:1.53E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:7.01|P-value:8.15E-10 ID:SGTC_547|Compound:1120-0019|FD-Score:-5.29|P-value:3.53E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:6.56|P-value:8.03E-9 ID:SGTC_553|Compound:k007-0256|FD-Score:11.60|P-value:1.59E-23 ID:SGTC_676|Compound:falcarinol|FD-Score:3.81|P-value:4.97E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.52|P-value:9.77E-7 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.71|P-value:6.67E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:4.96|P-value:9.40E-6 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.66|P-value:7.60E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.90|P-value:3.75E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.62|P-value:8.64E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-4.17|P-value:2.07E-4 ID:SGTC_1723|Compound:st034307|FD-Score:6.28|P-value:3.20E-8 ID:SGTC_1749|Compound:st037932|FD-Score:4.81|P-value:1.64E-5 ID:SGTC_1765|Compound:st044815|FD-Score:4.09|P-value:2.02E-4 ID:SGTC_1775|Compound:st081588|FD-Score:5.54|P-value:8.82E-7 ID:SGTC_1812|Compound:st052057|FD-Score:-4.08|P-value:2.79E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.95|P-value:3.20E-4 ID:SGTC_2044|Compound:5102272|FD-Score:4.38|P-value:7.69E-5 ID:SGTC_2124|Compound:5246320|FD-Score:13.90|P-value:2.93E-33 ID:SGTC_2168|Compound:5727979|FD-Score:4.32|P-value:9.56E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.76|P-value:2.01E-5 ID:SGTC_2231|Compound:micatex|FD-Score:3.77|P-value:5.49E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.79|P-value:5.15E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.18|P-value:1.53E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.30|P-value:1.00E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:9.67|P-value:4.82E-17 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:5.53|P-value:9.52E-7 ID:SGTC_2938|Compound:9059746|FD-Score:3.61|P-value:8.94E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.67|P-value:9.47E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-3.72|P-value:8.37E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-5.20|P-value:5.07E-6

Top fitness defect scores for YDR394W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2124 5246320 13.90 200.0 µM 2.93E-33 heme biosynthesis & mitochondrial translocase 3 1.93E-44 2.35
2 SGTC_553 k007-0256 11.60 45.7 µM 1.59E-23 heme biosynthesis & mitochondrial translocase 3 3.27E-31 2.74
3 SGTC_2409 alverine citrate 9.67 64.0 µM 4.82E-17 2 2.03E-22 1.40
4 SGTC_546 peri-Naphthalide 7.01 147.0 µM 8.15E-10 DNA damage response 5 1.22E-12 0.65
5 SGTC_550 4123-0385 6.56 25.7 µM 8.03E-9 TSC3-RPN4 1 2.67E-11 1.06
6 SGTC_1723 st034307 6.28 2.5 µM 3.20E-8 mitochondrial processes 5 1.72E-10 0.34
7 SGTC_1775 st081588 5.54 35.2 µM 8.82E-7 60S ribosome export 6 1.49E-8 0.06
8 SGTC_2617 dihydrotanshinone i 5.53 20.8 µM 9.52E-7 copper-dependent oxidative stress 10 1.65E-8 0.09
9 SGTC_1016 4226-1401 5.52 38.0 µM 9.77E-7 RNA pol III & RNase P/MRP 10 1.71E-8 0.22
10 SGTC_273 hydroxyurea 5.41 18.1 mM 1.53E-6 heme biosynthesis & mitochondrial translocase 7 3.12E-8 0.06
11 SGTC_1490 4089-0339 4.96 670.0 nM 9.40E-6 2 3.55E-7 0.87
12 SGTC_148 3253-0744 4.93 288.0 µM 1.05E-5 4 4.10E-7 0.62
13 SGTC_1749 st037932 4.81 54.2 µM 1.64E-5 3 7.47E-7 0.26
14 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 4.76 49.5 µM 2.01E-5 14 9.82E-7 0.07
15 SGTC_236 1509-0011 4.49 5.3 µM 5.28E-5 21 3.57E-6 0.04
16 SGTC_2044 5102272 4.38 200.0 µM 7.69E-5 5 5.90E-6 0.09
17 SGTC_2168 5727979 4.32 200.0 µM 9.56E-5 2 7.89E-6 0.30
18 SGTC_2406 ethylparaben 4.30 405.7 µM 1.00E-4 8 8.38E-6 0.49
19 SGTC_172 1112-0046 4.25 2.6 µM 1.21E-4 NEO1 6 1.09E-5 0.18
20 SGTC_2336 9001819 4.18 198.7 µM 1.53E-4 heme biosynthesis & mitochondrial translocase 34 1.47E-5 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.344 2.33E-93 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.326 8.70E-84 YOR259C RPT4 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.319 2.31E-80 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.270 5.12E-57 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.260 3.86E-53 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.222 7.73E-39 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.212 2.21E-35 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.189 2.12E-28 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.183 1.63E-26 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.172 9.19E-24 YMR290W-A_d YMR290W-A_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
0.160 1.43E-20 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.149 4.03E-18 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.147 1.30E-17 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.145 2.54E-17 YPR108W RPN7 het Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
0.141 3.24E-16 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress