YDR409W / SIZ1

SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR409W / SIZ1

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:4.24|P-value:1.35E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:4.52|P-value:3.96E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:5.98|P-value:1.69E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:3.16|P-value:8.67E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.36|P-value:4.76E-4 ID:SGTC_264|Compound:promethazine|FD-Score:4.52|P-value:4.01E-6 ID:SGTC_396|Compound:dithiazanine|FD-Score:5.90|P-value:2.83E-9 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.19|P-value:8.49E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.67|P-value:1.42E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.14|P-value:9.56E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:-3.15|P-value:9.79E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:3.54|P-value:2.26E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.16|P-value:2.11E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.55|P-value:2.20E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.37|P-value:4.33E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.31|P-value:5.29E-4 ID:SGTC_1519|Compound:esterone|FD-Score:4.42|P-value:6.07E-6 ID:SGTC_1726|Compound:st035556|FD-Score:-4.74|P-value:1.55E-6 ID:SGTC_1729|Compound:st035615|FD-Score:11.20|P-value:1.66E-28 ID:SGTC_1737|Compound:st035557|FD-Score:-3.26|P-value:6.70E-4 ID:SGTC_1952|Compound:st077036|FD-Score:4.42|P-value:6.09E-6 ID:SGTC_1968|Compound:st069590|FD-Score:5.12|P-value:2.12E-7 ID:SGTC_2045|Compound:5106652|FD-Score:3.39|P-value:4.00E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.52|P-value:2.47E-4 ID:SGTC_2143|Compound:5623677|FD-Score:3.92|P-value:5.24E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.26|P-value:1.37E-5 ID:SGTC_2340|Compound:7987427|FD-Score:3.61|P-value:1.76E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.43|P-value:5.95E-6 ID:SGTC_2343|Compound:7990716|FD-Score:-4.13|P-value:2.49E-5 ID:SGTC_2533|Compound:agelasine|FD-Score:3.72|P-value:1.18E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.52|P-value:2.66E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.34|P-value:4.81E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.47|P-value:2.93E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.92|P-value:5.37E-5 ID:SGTC_3040|Compound:9091894|FD-Score:3.42|P-value:3.59E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-3.74|P-value:1.19E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.24|P-value:1.35E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:4.52|P-value:3.96E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:5.98|P-value:1.69E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:3.16|P-value:8.67E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.36|P-value:4.76E-4 ID:SGTC_264|Compound:promethazine|FD-Score:4.52|P-value:4.01E-6 ID:SGTC_396|Compound:dithiazanine|FD-Score:5.90|P-value:2.83E-9 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.19|P-value:8.49E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.67|P-value:1.42E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.14|P-value:9.56E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:-3.15|P-value:9.79E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:3.54|P-value:2.26E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.16|P-value:2.11E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.55|P-value:2.20E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.37|P-value:4.33E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.31|P-value:5.29E-4 ID:SGTC_1519|Compound:esterone|FD-Score:4.42|P-value:6.07E-6 ID:SGTC_1726|Compound:st035556|FD-Score:-4.74|P-value:1.55E-6 ID:SGTC_1729|Compound:st035615|FD-Score:11.20|P-value:1.66E-28 ID:SGTC_1737|Compound:st035557|FD-Score:-3.26|P-value:6.70E-4 ID:SGTC_1952|Compound:st077036|FD-Score:4.42|P-value:6.09E-6 ID:SGTC_1968|Compound:st069590|FD-Score:5.12|P-value:2.12E-7 ID:SGTC_2045|Compound:5106652|FD-Score:3.39|P-value:4.00E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.52|P-value:2.47E-4 ID:SGTC_2143|Compound:5623677|FD-Score:3.92|P-value:5.24E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.26|P-value:1.37E-5 ID:SGTC_2340|Compound:7987427|FD-Score:3.61|P-value:1.76E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.43|P-value:5.95E-6 ID:SGTC_2343|Compound:7990716|FD-Score:-4.13|P-value:2.49E-5 ID:SGTC_2533|Compound:agelasine|FD-Score:3.72|P-value:1.18E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.52|P-value:2.66E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.34|P-value:4.81E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.47|P-value:2.93E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.92|P-value:5.37E-5 ID:SGTC_3040|Compound:9091894|FD-Score:3.42|P-value:3.59E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-3.74|P-value:1.19E-4

Top fitness defect scores for YDR409W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1729 st035615 11.20 46.0 µM 1.66E-28 1 3.38E-29
2 SGTC_145 3737-0113 5.98 38.4 µM 1.69E-9 DNA intercalators 28 1.10E-9
3 SGTC_396 dithiazanine 5.90 1.2 µM 2.83E-9 1 1.86E-9
4 SGTC_1968 st069590 5.12 43.6 µM 2.12E-7 5 1.55E-7
5 SGTC_143 4032-1194 4.52 15.7 µM 3.96E-6 39 3.12E-6
6 SGTC_264 promethazine 4.52 12.5 mM 4.01E-6 DNA intercalators 38 3.16E-6
7 SGTC_2341 7987486 4.43 176.7 µM 5.95E-6 11 4.74E-6
8 SGTC_1519 esterone 4.42 74.0 µM 6.07E-6 DNA intercalators 11 4.84E-6
9 SGTC_1952 st077036 4.42 64.1 µM 6.09E-6 22 4.85E-6
10 SGTC_43 0570-0105 4.24 72.5 µM 1.35E-5 ERG2 84 1.09E-5
11 SGTC_2143 5623677 3.92 140.5 µM 5.24E-5 5 4.39E-5
12 SGTC_2760 etidronate 3.92 98.0 µM 5.37E-5 28 4.50E-5
13 SGTC_2533 agelasine 3.72 13.7 µM 1.18E-4 ERG2 56 1.01E-4
14 SGTC_532 1548-0774 3.67 215.0 µM 1.42E-4 30 1.22E-4
15 SGTC_2340 7987427 3.61 183.4 µM 1.76E-4 52 1.52E-4
16 SGTC_1248 0469-0687 3.55 58.6 µM 2.20E-4 42 1.91E-4
17 SGTC_889 0919-1524 3.54 82.7 µM 2.26E-4 24 1.97E-4
18 SGTC_2052 5265182 3.52 7.1 µM 2.47E-4 44 2.15E-4
19 SGTC_2692 st077426 3.47 47.7 µM 2.93E-4 33 2.56E-4
20 SGTC_3040 9091894 3.42 49.5 µM 3.59E-4 DNA intercalators 32 3.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.241 9.69E-46 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.236 1.03E-43 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.232 2.50E-42 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.220 6.27E-38 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.219 9.28E-38 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.194 7.18E-30 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.190 1.02E-28 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.183 1.21E-26 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.177 3.96E-25 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.169 5.72E-23 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.169 6.43E-23 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.155 1.69E-19 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.151 1.23E-18 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.147 1.07E-17 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.142 1.63E-16 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin