YDR416W / SYF1

Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR416W / SYF1

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:-2.74|P-value:5.05E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:2.86|P-value:2.39E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-2.64|P-value:7.70E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.43|P-value:1.92E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.03|P-value:1.41E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.31|P-value:2.72E-5 ID:SGTC_489|Compound:paxilline|FD-Score:3.12|P-value:7.30E-5 ID:SGTC_529|Compound:4076-0302|FD-Score:2.96|P-value:1.55E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:4.97|P-value:7.77E-10 ID:SGTC_571|Compound:k831-0106|FD-Score:2.86|P-value:2.44E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:4.25|P-value:1.15E-7 ID:SGTC_981|Compound:1488-1094|FD-Score:3.00|P-value:1.28E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:5.88|P-value:4.62E-13 ID:SGTC_1030|Compound:k029-0042|FD-Score:-2.95|P-value:2.03E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:-2.79|P-value:4.20E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.50|P-value:2.20E-8 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.34|P-value:2.36E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:2.84|P-value:2.66E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.50|P-value:9.88E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:2.88|P-value:2.26E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.38|P-value:1.94E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.81|P-value:3.03E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:2.62|P-value:7.03E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:2.95|P-value:1.61E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:2.66|P-value:5.72E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:2.90|P-value:2.06E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:-2.77|P-value:4.51E-4 ID:SGTC_1591|Compound:genipin|FD-Score:2.71|P-value:4.68E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-2.78|P-value:4.35E-4 ID:SGTC_2354|Compound:9060492|FD-Score:6.44|P-value:2.69E-15 ID:SGTC_2355|Compound:9061122|FD-Score:2.86|P-value:2.44E-4 ID:SGTC_2356|Compound:9061756|FD-Score:2.59|P-value:7.78E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.80|P-value:3.21E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.02|P-value:1.46E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.14|P-value:6.50E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-2.82|P-value:3.57E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.77|P-value:1.83E-12 ID:SGTC_2967|Compound:9089538|FD-Score:3.70|P-value:3.34E-6 ID:SGTC_2974|Compound:9094440|FD-Score:3.61|P-value:5.58E-6 ID:SGTC_3209|Compound:9113616|FD-Score:2.60|P-value:7.63E-4 ID:SGTC_3322|Compound:9139058|FD-Score:-2.58|P-value:9.82E-4 ID:SGTC_3327|Compound:9140648|FD-Score:4.11|P-value:2.78E-7 ID:SGTC_7|Compound:0192-1582|FD-Score:-2.74|P-value:5.05E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:2.86|P-value:2.39E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-2.64|P-value:7.70E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.43|P-value:1.92E-5 ID:SGTC_260|Compound:nsc-65238|FD-Score:-3.03|P-value:1.41E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.31|P-value:2.72E-5 ID:SGTC_489|Compound:paxilline|FD-Score:3.12|P-value:7.30E-5 ID:SGTC_529|Compound:4076-0302|FD-Score:2.96|P-value:1.55E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:4.97|P-value:7.77E-10 ID:SGTC_571|Compound:k831-0106|FD-Score:2.86|P-value:2.44E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:4.25|P-value:1.15E-7 ID:SGTC_981|Compound:1488-1094|FD-Score:3.00|P-value:1.28E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:5.88|P-value:4.62E-13 ID:SGTC_1030|Compound:k029-0042|FD-Score:-2.95|P-value:2.03E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:-2.79|P-value:4.20E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.50|P-value:2.20E-8 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.34|P-value:2.36E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:2.84|P-value:2.66E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.50|P-value:9.88E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:2.88|P-value:2.26E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:3.38|P-value:1.94E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.81|P-value:3.03E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:2.62|P-value:7.03E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:2.95|P-value:1.61E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:2.66|P-value:5.72E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:2.90|P-value:2.06E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:-2.77|P-value:4.51E-4 ID:SGTC_1591|Compound:genipin|FD-Score:2.71|P-value:4.68E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-2.78|P-value:4.35E-4 ID:SGTC_2354|Compound:9060492|FD-Score:6.44|P-value:2.69E-15 ID:SGTC_2355|Compound:9061122|FD-Score:2.86|P-value:2.44E-4 ID:SGTC_2356|Compound:9061756|FD-Score:2.59|P-value:7.78E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.80|P-value:3.21E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.02|P-value:1.46E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.14|P-value:6.50E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-2.82|P-value:3.57E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.77|P-value:1.83E-12 ID:SGTC_2967|Compound:9089538|FD-Score:3.70|P-value:3.34E-6 ID:SGTC_2974|Compound:9094440|FD-Score:3.61|P-value:5.58E-6 ID:SGTC_3209|Compound:9113616|FD-Score:2.60|P-value:7.63E-4 ID:SGTC_3322|Compound:9139058|FD-Score:-2.58|P-value:9.82E-4 ID:SGTC_3327|Compound:9140648|FD-Score:4.11|P-value:2.78E-7

Top fitness defect scores for YDR416W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2354 9060492 6.44 162.0 µM 2.69E-15 2 5.99E-11 1.41
2 SGTC_1029 k015-0030 5.88 64.7 µM 4.62E-13 2 2.05E-9 1.26
3 SGTC_547 1120-0019 4.97 610.0 nM 7.77E-10 azole & statin 6 3.37E-7 1.01
4 SGTC_1043 0916-0009 4.50 19.3 µM 2.20E-8 4 3.37E-6 0.06
5 SGTC_704 1310-0270 4.25 142.0 µM 1.15E-7 3 1.05E-5 0.20
6 SGTC_3327 9140648 4.11 26.9 µM 2.78E-7 11 1.94E-5 0.10
7 SGTC_2967 9089538 3.70 49.9 µM 3.34E-6 16 1.08E-4 0.02
8 SGTC_2974 9094440 3.61 9.7 µM 5.58E-6 ergosterol biosynthesis 17 1.54E-4 0.01
9 SGTC_1185 1319-0113 3.50 81.3 µM 9.88E-6 DNA damage response 7 2.29E-4 0.19
10 SGTC_1219 1469-0211 3.38 33.1 µM 1.94E-5 8 3.65E-4 0.13
11 SGTC_1058 3448-5425 3.34 97.3 µM 2.36E-5 18 4.18E-4 0.05
12 SGTC_290 2043-6722 3.31 48.1 µM 2.72E-5 4 4.62E-4 0.01
13 SGTC_2772 vinpocetine 3.14 57.1 µM 6.50E-5 14 8.46E-4 0.10
14 SGTC_489 paxilline 3.12 115.0 µM 7.30E-5 PDR1 7 9.17E-4 0.03
15 SGTC_981 1488-1094 3.00 54.9 µM 1.28E-4 7 0.00135 0.11
16 SGTC_529 4076-0302 2.96 30.3 µM 1.55E-4 mitochondrial processes 14 0.00155 0.04
17 SGTC_1557 sumaresinolic acid 2.95 42.3 µM 1.61E-4 8 0.00159 0.29
18 SGTC_1574 spiramycin 2.90 23.7 µM 2.06E-4 22 0.00189 0.12
19 SGTC_1205 4161-2785 2.88 76.0 µM 2.26E-4 14 0.00201 0.06
20 SGTC_41 0478-0632 2.86 82.6 µM 2.39E-4 38 0.00210 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.142 1.47E-16 YML077W BET5 het Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
0.129 5.74E-14 YDR425W SNX41 hom Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p
0.125 3.83E-13 YLR397C AFG2 het ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
0.122 1.19E-12 YGR036C CAX4 hom Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
0.120 3.11E-12 YGL137W SEC27 het Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
0.117 1.14E-11 YDL184C RPL41A hom Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
0.116 1.60E-11 YKL196C YKT6 het Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
0.116 1.61E-11 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.114 4.23E-11 YDL164C CDC9 het DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
0.111 1.05E-10 YBL035C POL12 het B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
0.110 1.63E-10 YGL112C TAF6 het Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
0.107 4.99E-10 YDR021W FAL1 het Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
0.104 1.45E-9 YOR218C_d YOR218C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
0.101 5.36E-9 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.100 6.97E-9 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body