YDR418W / RPL12B

Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR418W / RPL12B

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-3.89|P-value:1.18E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.88|P-value:6.71E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:7.03|P-value:3.45E-12 ID:SGTC_159|Compound:3970-0959|FD-Score:3.13|P-value:9.86E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:5.31|P-value:9.74E-8 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.51|P-value:4.58E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.85|P-value:7.55E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.28|P-value:9.83E-4 ID:SGTC_389|Compound:tpck|FD-Score:3.93|P-value:5.48E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:3.15|P-value:9.16E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-4.98|P-value:1.12E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:4.38|P-value:8.29E-6 ID:SGTC_857|Compound:0438-0306|FD-Score:3.24|P-value:6.78E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:5.94|P-value:3.10E-9 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.50|P-value:4.85E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:-3.52|P-value:4.49E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:5.03|P-value:4.10E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.28|P-value:1.29E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:-3.47|P-value:5.27E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:4.93|P-value:6.49E-7 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.46|P-value:3.17E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.17|P-value:8.40E-4 ID:SGTC_1423|Compound:4048-0112|FD-Score:5.36|P-value:7.69E-8 ID:SGTC_1673|Compound:st016611|FD-Score:-3.50|P-value:4.73E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.72|P-value:2.18E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.91|P-value:5.88E-5 ID:SGTC_2154|Compound:5570129|FD-Score:3.59|P-value:2.00E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:4.08|P-value:2.99E-5 ID:SGTC_2325|Compound:6158266|FD-Score:3.19|P-value:8.02E-4 ID:SGTC_2391|Compound:6285546|FD-Score:3.32|P-value:5.24E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.22|P-value:7.35E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:4.35|P-value:9.52E-6 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-4.90|P-value:1.61E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:4.27|P-value:1.35E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.25|P-value:6.56E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.84|P-value:7.86E-5 ID:SGTC_2721|Compound:ifenprodil|FD-Score:-3.32|P-value:8.82E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:4.40|P-value:7.63E-6 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.41|P-value:6.52E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.15|P-value:9.05E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.94|P-value:5.24E-5 ID:SGTC_2861|Compound:9024595|FD-Score:3.62|P-value:1.75E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-4.91|P-value:1.53E-6 ID:SGTC_2870|Compound:9038528|FD-Score:4.57|P-value:3.51E-6 ID:SGTC_2873|Compound:9040596|FD-Score:3.24|P-value:6.81E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.23|P-value:7.06E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.19|P-value:8.11E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-6.09|P-value:3.27E-9 ID:SGTC_3129|Compound:9127257|FD-Score:4.88|P-value:8.31E-7 ID:SGTC_3145|Compound:9085516|FD-Score:-4.76|P-value:3.07E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.89|P-value:1.18E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.88|P-value:6.71E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:7.03|P-value:3.45E-12 ID:SGTC_159|Compound:3970-0959|FD-Score:3.13|P-value:9.86E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:5.31|P-value:9.74E-8 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.51|P-value:4.58E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.85|P-value:7.55E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.28|P-value:9.83E-4 ID:SGTC_389|Compound:tpck|FD-Score:3.93|P-value:5.48E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:3.15|P-value:9.16E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:-4.98|P-value:1.12E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:4.38|P-value:8.29E-6 ID:SGTC_857|Compound:0438-0306|FD-Score:3.24|P-value:6.78E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:5.94|P-value:3.10E-9 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.50|P-value:4.85E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:-3.52|P-value:4.49E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:5.03|P-value:4.10E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.28|P-value:1.29E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:-3.47|P-value:5.27E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:4.93|P-value:6.49E-7 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.46|P-value:3.17E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.17|P-value:8.40E-4 ID:SGTC_1423|Compound:4048-0112|FD-Score:5.36|P-value:7.69E-8 ID:SGTC_1673|Compound:st016611|FD-Score:-3.50|P-value:4.73E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.72|P-value:2.18E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.91|P-value:5.88E-5 ID:SGTC_2154|Compound:5570129|FD-Score:3.59|P-value:2.00E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:4.08|P-value:2.99E-5 ID:SGTC_2325|Compound:6158266|FD-Score:3.19|P-value:8.02E-4 ID:SGTC_2391|Compound:6285546|FD-Score:3.32|P-value:5.24E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.22|P-value:7.35E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:4.35|P-value:9.52E-6 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-4.90|P-value:1.61E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:4.27|P-value:1.35E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.25|P-value:6.56E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.84|P-value:7.86E-5 ID:SGTC_2721|Compound:ifenprodil|FD-Score:-3.32|P-value:8.82E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:4.40|P-value:7.63E-6 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.41|P-value:6.52E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.15|P-value:9.05E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.94|P-value:5.24E-5 ID:SGTC_2861|Compound:9024595|FD-Score:3.62|P-value:1.75E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-4.91|P-value:1.53E-6 ID:SGTC_2870|Compound:9038528|FD-Score:4.57|P-value:3.51E-6 ID:SGTC_2873|Compound:9040596|FD-Score:3.24|P-value:6.81E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.23|P-value:7.06E-4 ID:SGTC_3030|Compound:9087619|FD-Score:3.19|P-value:8.11E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-6.09|P-value:3.27E-9 ID:SGTC_3129|Compound:9127257|FD-Score:4.88|P-value:8.31E-7 ID:SGTC_3145|Compound:9085516|FD-Score:-4.76|P-value:3.07E-6

Top fitness defect scores for YDR418W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_118 0083-0097 7.03 135.3 µM 3.45E-12 translation 9 1.07E-12
2 SGTC_974 1326-0144 5.94 138.0 µM 3.10E-9 ERG2 11 1.43E-9
3 SGTC_1423 4048-0112 5.36 5.6 µM 7.69E-8 11 4.25E-8
4 SGTC_163 1327-0036 5.31 7.5 µM 9.74E-8 60 5.45E-8
5 SGTC_1150 3909-7983 5.03 10.9 µM 4.10E-7 7 2.48E-7
6 SGTC_1415 3966-0071 4.93 78.1 µM 6.49E-7 DNA intercalators 2 4.03E-7
7 SGTC_3129 9127257 4.88 49.5 µM 8.31E-7 6 5.23E-7
8 SGTC_2870 9038528 4.57 13.0 µM 3.51E-6 12 2.38E-6
9 SGTC_2730 imipramine 4.40 71.3 µM 7.63E-6 9 5.40E-6
10 SGTC_710 3448-4187 4.38 58.0 µM 8.29E-6 fatty acid desaturase (OLE1) 43 5.89E-6
11 SGTC_2568 diosmetin 4.35 100.0 µM 9.52E-6 18 6.82E-6
12 SGTC_1161 k007-0610 4.28 110.0 µM 1.29E-5 ERAD & cell cycle 108 9.37E-6
13 SGTC_2630 antimycin a 4.27 100.0 µM 1.35E-5 mitochondrial processes 29 9.83E-6
14 SGTC_2232 chlorophetanol 4.08 41.5 µM 2.99E-5 TRP & mitochondrial translation 118 2.27E-5
15 SGTC_2860 9025725 3.94 39.0 µM 5.24E-5 RPP1 & pyrimidine depletion 10 4.10E-5
16 SGTC_389 tpck 3.93 16.7 µM 5.48E-5 31 4.29E-5
17 SGTC_1724 st037281 3.91 8.3 µM 5.88E-5 46 4.62E-5
18 SGTC_103 0844-0104 3.88 51.8 µM 6.71E-5 ERAD & cell cycle 150 5.31E-5
19 SGTC_341 1486-1430 3.85 230.0 µM 7.55E-5 25 6.01E-5
20 SGTC_2688 kanamycin b 3.84 96.7 µM 7.86E-5 calcium & mitochondrial duress 390 6.26E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.159 1.65E-20 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.137 1.85E-15 YNL227C JJJ1 hom Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.135 4.55E-15 YPL210C SRP72 het Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
0.123 7.74E-13 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.118 6.75E-12 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.117 1.10E-11 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.116 1.95E-11 YMR142C RPL13B hom Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
0.115 2.07E-11 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.115 2.59E-11 YIL022W TIM44 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
0.114 3.91E-11 YGR135W PRE9 hom Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
0.114 4.11E-11 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.110 1.81E-10 YNL239W LAP3 hom Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
0.109 2.57E-10 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.107 4.47E-10 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.107 5.63E-10 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication