YDR427W / RPN9

Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR427W / RPN9

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:3.30|P-value:7.10E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.37|P-value:7.43E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.74|P-value:1.53E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:3.70|P-value:1.80E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.80|P-value:1.25E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.63|P-value:4.26E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:3.45|P-value:4.28E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.85|P-value:1.02E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.52|P-value:4.58E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:4.18|P-value:2.81E-5 ID:SGTC_989|Compound:1501-0895|FD-Score:3.87|P-value:9.49E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.59|P-value:3.84E-8 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.24|P-value:2.39E-7 ID:SGTC_1064|Compound:honokiol|FD-Score:3.62|P-value:2.40E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.59|P-value:5.07E-6 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:-3.93|P-value:1.05E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.84|P-value:1.64E-8 ID:SGTC_1113|Compound:artemisinin|FD-Score:7.47|P-value:4.03E-13 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.51|P-value:3.52E-4 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.90|P-value:8.64E-5 ID:SGTC_1503|Compound:4585-0012|FD-Score:4.50|P-value:7.30E-6 ID:SGTC_1797|Compound:st048695|FD-Score:4.27|P-value:1.96E-5 ID:SGTC_2016|Compound:5121758|FD-Score:4.09|P-value:4.05E-5 ID:SGTC_2124|Compound:5246320|FD-Score:8.31|P-value:8.27E-16 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.29|P-value:1.78E-5 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.36|P-value:7.80E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.38|P-value:5.49E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.66|P-value:2.85E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.65|P-value:2.18E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.94|P-value:7.32E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:4.22|P-value:2.41E-5 ID:SGTC_2537|Compound:larixol|FD-Score:5.29|P-value:1.81E-7 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.40|P-value:4.13E-10 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.33|P-value:6.55E-4 ID:SGTC_2852|Compound:9023854|FD-Score:-4.61|P-value:6.66E-6 ID:SGTC_2858|Compound:9025781|FD-Score:-5.64|P-value:4.71E-8 ID:SGTC_2922|Compound:7999257|FD-Score:-4.36|P-value:1.90E-5 ID:SGTC_2927|Compound:9011802|FD-Score:-4.10|P-value:5.56E-5 ID:SGTC_2928|Compound:9011484|FD-Score:5.65|P-value:2.86E-8 ID:SGTC_2959|Compound:9079155|FD-Score:4.02|P-value:5.43E-5 ID:SGTC_2963|Compound:9082507|FD-Score:5.04|P-value:6.27E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-3.53|P-value:4.46E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-3.43|P-value:6.16E-4 ID:SGTC_3030|Compound:9087619|FD-Score:4.01|P-value:5.51E-5 ID:SGTC_3091|Compound:9116372|FD-Score:-3.39|P-value:7.12E-4 ID:SGTC_3097|Compound:9117246|FD-Score:-3.90|P-value:1.18E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.96|P-value:9.23E-5 ID:SGTC_3142|Compound:9086498|FD-Score:5.01|P-value:7.31E-7 ID:SGTC_3197|Compound:9113331|FD-Score:3.23|P-value:9.09E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.28|P-value:7.75E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.85|P-value:1.04E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.30|P-value:7.10E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.37|P-value:7.43E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.74|P-value:1.53E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:3.70|P-value:1.80E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.80|P-value:1.25E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.63|P-value:4.26E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:3.45|P-value:4.28E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.85|P-value:1.02E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.52|P-value:4.58E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:4.18|P-value:2.81E-5 ID:SGTC_989|Compound:1501-0895|FD-Score:3.87|P-value:9.49E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.59|P-value:3.84E-8 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.24|P-value:2.39E-7 ID:SGTC_1064|Compound:honokiol|FD-Score:3.62|P-value:2.40E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.59|P-value:5.07E-6 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:-3.93|P-value:1.05E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.84|P-value:1.64E-8 ID:SGTC_1113|Compound:artemisinin|FD-Score:7.47|P-value:4.03E-13 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.51|P-value:3.52E-4 ID:SGTC_1477|Compound:0139-0234|FD-Score:3.90|P-value:8.64E-5 ID:SGTC_1503|Compound:4585-0012|FD-Score:4.50|P-value:7.30E-6 ID:SGTC_1797|Compound:st048695|FD-Score:4.27|P-value:1.96E-5 ID:SGTC_2016|Compound:5121758|FD-Score:4.09|P-value:4.05E-5 ID:SGTC_2124|Compound:5246320|FD-Score:8.31|P-value:8.27E-16 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.29|P-value:1.78E-5 ID:SGTC_2131|Compound:toluylene red|FD-Score:-3.36|P-value:7.80E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.38|P-value:5.49E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.66|P-value:2.85E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.65|P-value:2.18E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.94|P-value:7.32E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:4.22|P-value:2.41E-5 ID:SGTC_2537|Compound:larixol|FD-Score:5.29|P-value:1.81E-7 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.40|P-value:4.13E-10 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.33|P-value:6.55E-4 ID:SGTC_2852|Compound:9023854|FD-Score:-4.61|P-value:6.66E-6 ID:SGTC_2858|Compound:9025781|FD-Score:-5.64|P-value:4.71E-8 ID:SGTC_2922|Compound:7999257|FD-Score:-4.36|P-value:1.90E-5 ID:SGTC_2927|Compound:9011802|FD-Score:-4.10|P-value:5.56E-5 ID:SGTC_2928|Compound:9011484|FD-Score:5.65|P-value:2.86E-8 ID:SGTC_2959|Compound:9079155|FD-Score:4.02|P-value:5.43E-5 ID:SGTC_2963|Compound:9082507|FD-Score:5.04|P-value:6.27E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-3.53|P-value:4.46E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-3.43|P-value:6.16E-4 ID:SGTC_3030|Compound:9087619|FD-Score:4.01|P-value:5.51E-5 ID:SGTC_3091|Compound:9116372|FD-Score:-3.39|P-value:7.12E-4 ID:SGTC_3097|Compound:9117246|FD-Score:-3.90|P-value:1.18E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.96|P-value:9.23E-5 ID:SGTC_3142|Compound:9086498|FD-Score:5.01|P-value:7.31E-7 ID:SGTC_3197|Compound:9113331|FD-Score:3.23|P-value:9.09E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.28|P-value:7.75E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.85|P-value:1.04E-4

Top fitness defect scores for YDR427W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2124 5246320 8.31 200.0 µM 8.27E-16 heme biosynthesis & mitochondrial translocase 10 4.81E-17 0.30
2 SGTC_1113 artemisinin 7.47 1.2 µM 4.03E-13 heme biosynthesis & mitochondrial translocase 10 4.15E-14 0.83
3 SGTC_2575 plumbagin 6.40 510.0 nM 4.13E-10 superoxide 9 8.00E-11 0.27
4 SGTC_2928 9011484 5.65 9.8 µM 2.86E-8 heme biosynthesis & mitochondrial translocase 12 8.08E-9 0.07
5 SGTC_1016 4226-1401 5.59 38.0 µM 3.84E-8 RNA pol III & RNase P/MRP 9 1.11E-8 0.07
6 SGTC_2537 larixol 5.29 68.9 µM 1.81E-7 3 6.03E-8 0.98
7 SGTC_1044 1222-0021 5.24 1.8 µM 2.39E-7 heme biosynthesis & mitochondrial translocase 19 8.18E-8 0.02
8 SGTC_2963 9082507 5.04 9.0 µM 6.27E-7 4 2.33E-7 0.90
9 SGTC_3142 9086498 5.01 49.5 µM 7.31E-7 iron homeostasis 30 2.75E-7 0.08
10 SGTC_431 k089-0097 4.63 107.0 µM 4.26E-6 iron homeostasis 7 1.87E-6 0.05
11 SGTC_1098 clofazimine 4.59 15.1 µM 5.07E-6 heme biosynthesis & mitochondrial translocase 22 2.26E-6 0.02
12 SGTC_1503 4585-0012 4.50 61.3 µM 7.30E-6 5 3.36E-6 0.64
13 SGTC_2129 girgensonine 4.29 121.0 µM 1.78E-5 heme biosynthesis & mitochondrial translocase 18 8.82E-6 0.05
14 SGTC_1797 st048695 4.27 56.8 µM 1.96E-5 NEO1 11 9.80E-6 0.02
15 SGTC_2519 cryptotanshinone 4.22 88.0 µM 2.41E-5 heme biosynthesis & mitochondrial translocase 18 1.23E-5 0.46
16 SGTC_975 1335-0046 4.18 28.5 µM 2.81E-5 RPP1 & pyrimidine depletion 5 1.45E-5 0.05
17 SGTC_2016 5121758 4.09 140.0 µM 4.05E-5 redox potentiating 12 2.15E-5 0.01
18 SGTC_2959 9079155 4.02 11.0 µM 5.43E-5 9 2.96E-5 0.18
19 SGTC_3030 9087619 4.01 49.5 µM 5.51E-5 7 3.01E-5 0.15
20 SGTC_2348 9022543 3.94 33.4 µM 7.32E-5 mitochondrial processes 31 4.09E-5 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.309 6.95E-75 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.287 1.65E-64 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.277 5.30E-60 YDL147W RPN5 het Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
0.272 7.13E-58 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.264 1.57E-54 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.242 9.11E-46 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.215 2.19E-36 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.204 7.98E-33 YIL075C RPN2 het Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
0.196 2.64E-30 YEL034W HYP2 het Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.195 6.20E-30 YFR004W RPN11 het Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.189 2.12E-28 YDR394W RPT3 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
0.176 1.06E-24 YDL097C RPN6 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
0.174 2.59E-24 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.170 4.42E-23 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.165 6.17E-22 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus