YDR432W / NPL3

RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR432W / NPL3

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.51|P-value:7.50E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:4.49|P-value:2.42E-5 ID:SGTC_31|Compound:0508-1974|FD-Score:5.09|P-value:1.96E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:3.44|P-value:9.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.36|P-value:4.02E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:5.35|P-value:6.11E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:4.49|P-value:2.43E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:8.23|P-value:3.84E-14 ID:SGTC_214|Compound:4373-3496|FD-Score:3.52|P-value:7.31E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:3.73|P-value:3.69E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.31|P-value:3.62E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.15|P-value:8.55E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:4.52|P-value:2.16E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.36|P-value:9.64E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:7.28|P-value:1.94E-11 ID:SGTC_428|Compound:3448-4153|FD-Score:3.62|P-value:5.41E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.05|P-value:1.72E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:4.08|P-value:1.13E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:3.93|P-value:1.87E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.44|P-value:9.39E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:3.51|P-value:7.64E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:5.74|P-value:9.85E-8 ID:SGTC_704|Compound:1310-0270|FD-Score:-4.55|P-value:1.44E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.37|P-value:9.60E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.51|P-value:7.65E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:7.85|P-value:4.97E-13 ID:SGTC_878|Compound:0828-0289|FD-Score:4.24|P-value:6.28E-5 ID:SGTC_886|Compound:0866-0150|FD-Score:4.41|P-value:3.30E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:4.77|P-value:7.79E-6 ID:SGTC_902|Compound:1120-0002|FD-Score:-3.53|P-value:5.66E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.27|P-value:5.57E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:6.79|P-value:3.52E-10 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.63|P-value:5.18E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.45|P-value:9.05E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.47|P-value:8.70E-4 ID:SGTC_1604|Compound:st002052|FD-Score:-5.34|P-value:4.77E-7 ID:SGTC_1606|Compound:st000094|FD-Score:3.93|P-value:1.89E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.68|P-value:1.14E-5 ID:SGTC_1613|Compound:st002381|FD-Score:3.46|P-value:8.84E-4 ID:SGTC_1617|Compound:st002906|FD-Score:3.83|P-value:2.70E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.76|P-value:3.38E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.77|P-value:7.89E-6 ID:SGTC_1765|Compound:st044815|FD-Score:-4.09|P-value:8.53E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.88|P-value:2.23E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.47|P-value:2.62E-5 ID:SGTC_1846|Compound:st056244|FD-Score:4.11|P-value:9.84E-5 ID:SGTC_1855|Compound:st056195|FD-Score:-4.66|P-value:9.12E-6 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.66|P-value:4.70E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.88|P-value:2.27E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.58|P-value:6.11E-4 ID:SGTC_2177|Compound:5811945|FD-Score:-3.39|P-value:9.05E-4 ID:SGTC_2357|Compound:9067248|FD-Score:4.24|P-value:6.23E-5 ID:SGTC_2401|Compound:5128683|FD-Score:3.90|P-value:2.10E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.52|P-value:7.25E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:4.61|P-value:1.49E-5 ID:SGTC_2676|Compound:harmalol|FD-Score:4.35|P-value:4.09E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.36|P-value:3.82E-9 ID:SGTC_2743|Compound:auranofin|FD-Score:4.00|P-value:1.47E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:4.96|P-value:3.56E-6 ID:SGTC_2812|Compound:7970281|FD-Score:4.16|P-value:8.50E-5 ID:SGTC_2892|Compound:9047358|FD-Score:4.68|P-value:1.14E-5 ID:SGTC_2938|Compound:9059746|FD-Score:5.74|P-value:9.64E-8 ID:SGTC_2944|Compound:9067346|FD-Score:3.53|P-value:7.14E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.71|P-value:1.14E-7 ID:SGTC_2950|Compound:9072895|FD-Score:3.92|P-value:1.96E-4 ID:SGTC_2952|Compound:9073731|FD-Score:4.07|P-value:1.15E-4 ID:SGTC_3101|Compound:9120531|FD-Score:4.02|P-value:1.37E-4 ID:SGTC_3106|Compound:9121286|FD-Score:-3.40|P-value:8.58E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.56|P-value:6.42E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.98|P-value:1.58E-4 ID:SGTC_3337|Compound:9144342|FD-Score:8.75|P-value:9.40E-16 ID:SGTC_17|Compound:0960-0276|FD-Score:3.51|P-value:7.50E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:4.49|P-value:2.42E-5 ID:SGTC_31|Compound:0508-1974|FD-Score:5.09|P-value:1.96E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:3.44|P-value:9.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.36|P-value:4.02E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:5.35|P-value:6.11E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:4.49|P-value:2.43E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:8.23|P-value:3.84E-14 ID:SGTC_214|Compound:4373-3496|FD-Score:3.52|P-value:7.31E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:3.73|P-value:3.69E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.31|P-value:3.62E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:4.15|P-value:8.55E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:4.52|P-value:2.16E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.36|P-value:9.64E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:7.28|P-value:1.94E-11 ID:SGTC_428|Compound:3448-4153|FD-Score:3.62|P-value:5.41E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.05|P-value:1.72E-6 ID:SGTC_515|Compound:4092-1064|FD-Score:4.08|P-value:1.13E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:3.93|P-value:1.87E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.44|P-value:9.39E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:3.51|P-value:7.64E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:5.74|P-value:9.85E-8 ID:SGTC_704|Compound:1310-0270|FD-Score:-4.55|P-value:1.44E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.37|P-value:9.60E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:3.51|P-value:7.65E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:7.85|P-value:4.97E-13 ID:SGTC_878|Compound:0828-0289|FD-Score:4.24|P-value:6.28E-5 ID:SGTC_886|Compound:0866-0150|FD-Score:4.41|P-value:3.30E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:4.77|P-value:7.79E-6 ID:SGTC_902|Compound:1120-0002|FD-Score:-3.53|P-value:5.66E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.27|P-value:5.57E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:6.79|P-value:3.52E-10 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.63|P-value:5.18E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.45|P-value:9.05E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:3.47|P-value:8.70E-4 ID:SGTC_1604|Compound:st002052|FD-Score:-5.34|P-value:4.77E-7 ID:SGTC_1606|Compound:st000094|FD-Score:3.93|P-value:1.89E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.68|P-value:1.14E-5 ID:SGTC_1613|Compound:st002381|FD-Score:3.46|P-value:8.84E-4 ID:SGTC_1617|Compound:st002906|FD-Score:3.83|P-value:2.70E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.76|P-value:3.38E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.77|P-value:7.89E-6 ID:SGTC_1765|Compound:st044815|FD-Score:-4.09|P-value:8.53E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.88|P-value:2.23E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.47|P-value:2.62E-5 ID:SGTC_1846|Compound:st056244|FD-Score:4.11|P-value:9.84E-5 ID:SGTC_1855|Compound:st056195|FD-Score:-4.66|P-value:9.12E-6 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.66|P-value:4.70E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.88|P-value:2.27E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.58|P-value:6.11E-4 ID:SGTC_2177|Compound:5811945|FD-Score:-3.39|P-value:9.05E-4 ID:SGTC_2357|Compound:9067248|FD-Score:4.24|P-value:6.23E-5 ID:SGTC_2401|Compound:5128683|FD-Score:3.90|P-value:2.10E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.52|P-value:7.25E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:4.61|P-value:1.49E-5 ID:SGTC_2676|Compound:harmalol|FD-Score:4.35|P-value:4.09E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.36|P-value:3.82E-9 ID:SGTC_2743|Compound:auranofin|FD-Score:4.00|P-value:1.47E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:4.96|P-value:3.56E-6 ID:SGTC_2812|Compound:7970281|FD-Score:4.16|P-value:8.50E-5 ID:SGTC_2892|Compound:9047358|FD-Score:4.68|P-value:1.14E-5 ID:SGTC_2938|Compound:9059746|FD-Score:5.74|P-value:9.64E-8 ID:SGTC_2944|Compound:9067346|FD-Score:3.53|P-value:7.14E-4 ID:SGTC_2948|Compound:9070189|FD-Score:5.71|P-value:1.14E-7 ID:SGTC_2950|Compound:9072895|FD-Score:3.92|P-value:1.96E-4 ID:SGTC_2952|Compound:9073731|FD-Score:4.07|P-value:1.15E-4 ID:SGTC_3101|Compound:9120531|FD-Score:4.02|P-value:1.37E-4 ID:SGTC_3106|Compound:9121286|FD-Score:-3.40|P-value:8.58E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.56|P-value:6.42E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.98|P-value:1.58E-4 ID:SGTC_3337|Compound:9144342|FD-Score:8.75|P-value:9.40E-16

Top fitness defect scores for YDR432W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3337 9144342 8.75 33.5 µM 9.40E-16 6 1.10E-18
2 SGTC_168 3970-0862 8.23 530.5 µM 3.84E-14 18 9.54E-17
3 SGTC_828 2074-0507 7.85 139.0 µM 4.97E-13 RPP1 & pyrimidine depletion 4 2.08E-15
4 SGTC_377 3970-0964 7.28 33.1 µM 1.94E-11 7 1.71E-13
5 SGTC_1132 3456-3614 6.79 2.5 µM 3.52E-10 tubulin folding & SWR complex 8 5.56E-12
6 SGTC_2679 rapamycin 6.36 1.0 nM 3.82E-9 68 9.77E-11
7 SGTC_58 0957-0393 6.36 9.0 µM 4.02E-9 cell wall 189 1.04E-10
8 SGTC_2938 9059746 5.74 27.3 µM 9.64E-8 fatty acid desaturase (OLE1) 15 4.73E-9
9 SGTC_637 1181-0519 5.74 7.3 µM 9.85E-8 fatty acid desaturase (OLE1) 57 4.85E-9
10 SGTC_2948 9070189 5.71 9.4 µM 1.14E-7 plasma membrane duress 25 5.81E-9
11 SGTC_146 3448-8650 5.35 53.1 µM 6.11E-7 NEO1-PIK1 64 4.35E-8
12 SGTC_31 0508-1974 5.09 57.2 µM 1.96E-6 8 1.76E-7
13 SGTC_2771 fluoxetine 4.96 41.0 µM 3.56E-6 8 3.61E-7
14 SGTC_893 0958-0369 4.77 48.9 µM 7.79E-6 15 9.24E-7
15 SGTC_1687 st020778 4.77 55.4 µM 7.89E-6 140 9.39E-7
16 SGTC_2892 9047358 4.68 58.4 µM 1.14E-5 3 1.46E-6
17 SGTC_1607 st000305 4.68 50.6 µM 1.14E-5 3 1.46E-6
18 SGTC_2595 hexamethylquercetagetin 4.61 100.0 µM 1.49E-5 12 2.02E-6
19 SGTC_366 0181-0206 4.52 4.3 µM 2.16E-5 Golgi 24 3.15E-6
20 SGTC_22 0964-0034 4.49 14.5 µM 2.42E-5 unfolded protein response 35 3.60E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 3.49E-23 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.163 3.02E-21 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.155 2.35E-19 YDR433W_d YDR433W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.151 1.69E-18 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.150 2.06E-18 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.149 3.39E-18 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.149 3.54E-18 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.147 1.21E-17 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.144 4.98E-17 YLR347C KAP95 het Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
0.136 3.34E-15 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.135 4.22E-15 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.134 6.00E-15 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.134 6.68E-15 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.133 1.03E-14 YDL041W_d YDL041W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
0.130 4.13E-14 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus