YDR440W / DOT1

Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR440W / DOT1

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-4.07|P-value:2.29E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:-3.46|P-value:2.58E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:3.25|P-value:6.01E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.18|P-value:7.67E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.79|P-value:8.04E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:5.09|P-value:1.95E-7 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.42|P-value:2.99E-4 ID:SGTC_470|Compound:aa-861|FD-Score:-3.94|P-value:3.99E-5 ID:SGTC_491|Compound:tetrandrine|FD-Score:4.99|P-value:3.24E-7 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.24|P-value:5.69E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:4.24|P-value:1.16E-5 ID:SGTC_749|Compound:1000-0129|FD-Score:-3.23|P-value:5.97E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.33|P-value:4.46E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.85|P-value:6.21E-5 ID:SGTC_912|Compound:3474-0017|FD-Score:4.12|P-value:1.96E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.15|P-value:7.84E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.25|P-value:6.09E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.41|P-value:3.44E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.64|P-value:1.43E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:5.22|P-value:9.45E-8 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.65|P-value:1.35E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.54|P-value:2.06E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.54|P-value:2.11E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.61|P-value:1.48E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-4.00|P-value:3.06E-5 ID:SGTC_1637|Compound:st007924|FD-Score:-4.05|P-value:2.43E-5 ID:SGTC_1638|Compound:st008370|FD-Score:-4.72|P-value:1.13E-6 ID:SGTC_1672|Compound:st016598|FD-Score:3.21|P-value:6.85E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.34|P-value:4.32E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.09|P-value:9.66E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.31|P-value:4.52E-4 ID:SGTC_1900|Compound:st060200|FD-Score:-3.34|P-value:4.03E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-4.05|P-value:2.49E-5 ID:SGTC_2112|Compound:5488546|FD-Score:-3.31|P-value:4.51E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-4.53|P-value:2.86E-6 ID:SGTC_2326|Compound:6164455|FD-Score:4.37|P-value:6.53E-6 ID:SGTC_2328|Compound:6201408|FD-Score:-7.68|P-value:7.73E-15 ID:SGTC_2329|Compound:6138490|FD-Score:-3.45|P-value:2.66E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.50|P-value:2.23E-4 ID:SGTC_2343|Compound:7990716|FD-Score:4.40|P-value:5.71E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:4.30|P-value:9.16E-6 ID:SGTC_2691|Compound:st077356|FD-Score:4.43|P-value:5.01E-6 ID:SGTC_2692|Compound:st077426|FD-Score:-3.76|P-value:8.14E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.58|P-value:2.44E-6 ID:SGTC_2698|Compound:st077697|FD-Score:-4.73|P-value:1.09E-6 ID:SGTC_2699|Compound:st077704|FD-Score:4.85|P-value:6.59E-7 ID:SGTC_2704|Compound:st078022|FD-Score:5.90|P-value:1.94E-9 ID:SGTC_2707|Compound:st078556|FD-Score:5.42|P-value:3.12E-8 ID:SGTC_2713|Compound:st079149|FD-Score:7.40|P-value:7.32E-14 ID:SGTC_2715|Compound:st079500|FD-Score:-10.40|P-value:7.56E-26 ID:SGTC_3049|Compound:9092223|FD-Score:-3.35|P-value:3.93E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-3.24|P-value:5.82E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.33|P-value:4.47E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.18|P-value:7.75E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.40|P-value:3.55E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.07|P-value:2.29E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:-3.46|P-value:2.58E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:3.25|P-value:6.01E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.18|P-value:7.67E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.79|P-value:8.04E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:5.09|P-value:1.95E-7 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.42|P-value:2.99E-4 ID:SGTC_470|Compound:aa-861|FD-Score:-3.94|P-value:3.99E-5 ID:SGTC_491|Compound:tetrandrine|FD-Score:4.99|P-value:3.24E-7 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.24|P-value:5.69E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:4.24|P-value:1.16E-5 ID:SGTC_749|Compound:1000-0129|FD-Score:-3.23|P-value:5.97E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.33|P-value:4.46E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.85|P-value:6.21E-5 ID:SGTC_912|Compound:3474-0017|FD-Score:4.12|P-value:1.96E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.15|P-value:7.84E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.25|P-value:6.09E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.41|P-value:3.44E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.64|P-value:1.43E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:5.22|P-value:9.45E-8 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.65|P-value:1.35E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.54|P-value:2.06E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.54|P-value:2.11E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.61|P-value:1.48E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-4.00|P-value:3.06E-5 ID:SGTC_1637|Compound:st007924|FD-Score:-4.05|P-value:2.43E-5 ID:SGTC_1638|Compound:st008370|FD-Score:-4.72|P-value:1.13E-6 ID:SGTC_1672|Compound:st016598|FD-Score:3.21|P-value:6.85E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.34|P-value:4.32E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.09|P-value:9.66E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.31|P-value:4.52E-4 ID:SGTC_1900|Compound:st060200|FD-Score:-3.34|P-value:4.03E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-4.05|P-value:2.49E-5 ID:SGTC_2112|Compound:5488546|FD-Score:-3.31|P-value:4.51E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-4.53|P-value:2.86E-6 ID:SGTC_2326|Compound:6164455|FD-Score:4.37|P-value:6.53E-6 ID:SGTC_2328|Compound:6201408|FD-Score:-7.68|P-value:7.73E-15 ID:SGTC_2329|Compound:6138490|FD-Score:-3.45|P-value:2.66E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.50|P-value:2.23E-4 ID:SGTC_2343|Compound:7990716|FD-Score:4.40|P-value:5.71E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:4.30|P-value:9.16E-6 ID:SGTC_2691|Compound:st077356|FD-Score:4.43|P-value:5.01E-6 ID:SGTC_2692|Compound:st077426|FD-Score:-3.76|P-value:8.14E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.58|P-value:2.44E-6 ID:SGTC_2698|Compound:st077697|FD-Score:-4.73|P-value:1.09E-6 ID:SGTC_2699|Compound:st077704|FD-Score:4.85|P-value:6.59E-7 ID:SGTC_2704|Compound:st078022|FD-Score:5.90|P-value:1.94E-9 ID:SGTC_2707|Compound:st078556|FD-Score:5.42|P-value:3.12E-8 ID:SGTC_2713|Compound:st079149|FD-Score:7.40|P-value:7.32E-14 ID:SGTC_2715|Compound:st079500|FD-Score:-10.40|P-value:7.56E-26 ID:SGTC_3049|Compound:9092223|FD-Score:-3.35|P-value:3.93E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-3.24|P-value:5.82E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.33|P-value:4.47E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.18|P-value:7.75E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.40|P-value:3.55E-4

Top fitness defect scores for YDR440W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2713 st079149 7.40 53.4 µM 7.32E-14 1 6.67E-14
2 SGTC_2704 st078022 5.90 71.1 µM 1.94E-9 4 1.80E-9
3 SGTC_2707 st078556 5.42 66.6 µM 3.12E-8 3 2.92E-8
4 SGTC_1130 3454-2858 5.22 15.9 µM 9.45E-8 NEO1 54 8.86E-8
5 SGTC_390 dec-rvkr-cmk 5.09 16.7 µM 1.95E-7 calcium & mitochondrial duress 10 1.84E-7
6 SGTC_491 tetrandrine 4.99 80.3 µM 3.24E-7 2 3.05E-7
7 SGTC_2699 st077704 4.85 54.6 µM 6.59E-7 endomembrane recycling 30 6.21E-7
8 SGTC_2697 mebhydrolin 4.58 72.4 µM 2.44E-6 NEO1 51 2.31E-6
9 SGTC_2691 st077356 4.43 60.2 µM 5.01E-6 17 4.75E-6
10 SGTC_2343 7990716 4.40 14.8 µM 5.71E-6 11 5.41E-6
11 SGTC_2326 6164455 4.37 177.6 µM 6.53E-6 mitochondrial processes 15 6.19E-6
12 SGTC_2620 irigenin 4.30 100.0 µM 9.16E-6 27 8.70E-6
13 SGTC_651 3253-0966 4.24 34.4 µM 1.16E-5 calcium & mitochondrial duress 26 1.10E-5
14 SGTC_912 3474-0017 4.12 2.2 µM 1.96E-5 19 1.87E-5
15 SGTC_909 3448-9333 3.85 149.0 µM 6.21E-5 19 5.93E-5
16 SGTC_382 3229-0966 3.79 23.2 µM 8.04E-5 26 7.68E-5
17 SGTC_1348 1493-0373 3.65 93.8 µM 1.35E-4 19 1.29E-4
18 SGTC_1111 tnp00062 3.64 510.7 nM 1.43E-4 14 1.37E-4
19 SGTC_1597 7-benzyloxygramine 3.54 47.5 µM 2.06E-4 calcium & mitochondrial duress 153 1.97E-4
20 SGTC_1628 st004252 3.54 77.7 µM 2.11E-4 34 2.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.100 7.59E-9 YCR012W PGK1 het 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
0.095 3.87E-8 YJL212C OPT1 hom Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
0.095 4.39E-8 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.093 6.61E-8 YKL094W YJU3 hom Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
0.093 7.85E-8 YPR096C YPR096C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments
0.092 1.10E-7 YGR108W CLB1 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome
0.090 2.00E-7 YJR152W DAL5 hom Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
0.088 3.22E-7 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.088 3.84E-7 YFR017C IGD1 hom Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication
0.087 5.19E-7 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.082 2.06E-6 YGR177C ATF2 hom Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
0.081 2.77E-6 YKL035W UGP1 het UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
0.081 2.82E-6 YGR246C BRF1 het TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
0.080 3.35E-6 YKR032W_d YKR032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.078 6.21E-6 YJR041C URB2 het Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis