HIPHOP chemogenomics database

YDR457W / TOM1

E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR457W / TOM1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR457W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	11.50	4.6 µM	2.02E-34	heat shock/prefoldin	5	5.29E-31
2	SGTC_641	4092-0595	11.10	30.0 µM	5.57E-32		1	8.26E-29
3	SGTC_238	5hr heat shock (37°C) + nsc-638432	10.30	8.9 µM	8.87E-28	heat shock/prefoldin	5	4.95E-25
4	SGTC_3337	9144342	10.00	33.5 µM	1.72E-26		3	7.11E-24
5	SGTC_241	5hr heat shock (37°C) + cantharidin	9.52	155.4 µM	3.53E-24	cell wall signaling	12	8.52E-22
6	SGTC_239	5hr heat shock (37°C) + mitomycin C	9.34	819.4 µM	2.41E-23	heat shock/prefoldin	5	4.77E-21
7	SGTC_2751	anetholtrithion	9.14	17.2 µM	1.86E-22		1	3.00E-20
8	SGTC_62	0180-0423	8.21	3.2 µM	1.75E-18	60S ribosome export	2	1.11E-16
9	SGTC_240	5hr heat shock (37°C) + Streptovitacin	7.88	300.0 µM	3.58E-17	heat shock/prefoldin	9	1.67E-15
10	SGTC_243	5hr heat shock (37°C) + splitomicin	7.61	1.0 µM	3.79E-16	heat shock/prefoldin	10	1.40E-14
11	SGTC_212	3807-4682	6.23	38.8 µM	1.87E-11	tubulin folding & SWR complex	23	2.28E-10
12	SGTC_77	0828-0286	6.14	12.5 µM	3.66E-11	TSC3-RPN4	4	4.17E-10
13	SGTC_2933	9026794	5.91	32.5 µM	1.71E-10		3	1.66E-9
14	SGTC_1029	k015-0030	5.44	64.7 µM	3.70E-9		10	2.63E-8
15	SGTC_2945	9067681	5.07	61.8 µM	3.62E-8		7	2.03E-7
16	SGTC_2753	fluvastatin	5.03	17.1 µM	4.42E-8	azole & statin	15	2.43E-7
17	SGTC_1043	0916-0009	4.86	19.3 µM	1.21E-7		7	6.02E-7
18	SGTC_2315	7733312	4.73	200.0 µM	2.45E-7		12	1.13E-6
19	SGTC_1040	k898-0338	4.68	155.0 µM	3.22E-7		10	1.44E-6
20	SGTC_2949	9072542	4.62	2.2 µM	4.40E-7	TSC3-RPN4	22	1.91E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.357	2.08E-101	YLR027C	AAT2	hom	Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.348	6.11E-96	YOR001W	RRP6	hom	Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.344	1.05E-93	YDR260C	SWM1	hom	Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.280	1.30E-61	YLR200W	YKE2	hom	Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.262	8.36E-54	YCR063W	BUD31	hom	Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.246	1.23E-47	YNL140C_p	YNL140C_p	hom	Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
0.195	3.35E-30	YNL098C	RAS2	hom	GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.190	1.45E-28	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.181	3.44E-26	YCR077C	PAT1	hom	Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
0.174	2.33E-24	YAL047C	SPC72	hom	Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization
0.169	8.19E-23	YKL007W	CAP1	hom	Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
0.167	2.28E-22	YPL017C	IRC15	hom	Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.165	7.37E-22	YML094C-A_d	YML094C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.153	4.87E-19	YHR041C	SRB2	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.141	2.52E-16	YCR064C_d	YCR064C_d	hom	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31