YDR462W / MRPL28

Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR462W / MRPL28

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:7.24|P-value:1.40E-12 ID:SGTC_19|Compound:0279-0214|FD-Score:5.31|P-value:1.47E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:6.69|P-value:5.20E-11 ID:SGTC_116|Compound:0155-0182|FD-Score:4.10|P-value:3.70E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.84|P-value:1.03E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:5.26|P-value:1.87E-7 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.27|P-value:7.76E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:5.33|P-value:1.29E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:4.07|P-value:4.06E-5 ID:SGTC_161|Compound:4065-0411|FD-Score:3.65|P-value:2.10E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:6.02|P-value:3.05E-9 ID:SGTC_173|Compound:1273-0061|FD-Score:12.70|P-value:6.83E-35 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.38|P-value:5.82E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:28.90|P-value:3.02E-171 ID:SGTC_275|Compound:trichlorophene|FD-Score:12.90|P-value:6.51E-36 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.69|P-value:1.97E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:6.17|P-value:1.24E-9 ID:SGTC_314|Compound:1671-0008|FD-Score:3.80|P-value:1.19E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.46|P-value:8.93E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:6.21|P-value:9.85E-10 ID:SGTC_431|Compound:k089-0097|FD-Score:-6.77|P-value:3.82E-11 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.86|P-value:9.49E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:4.01|P-value:5.30E-5 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.23|P-value:8.97E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:4.92|P-value:9.98E-7 ID:SGTC_602|Compound:3130-1533|FD-Score:4.01|P-value:5.27E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:4.92|P-value:9.77E-7 ID:SGTC_614|Compound:0462-0418|FD-Score:3.51|P-value:3.37E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.46|P-value:4.42E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-4.31|P-value:1.76E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:4.33|P-value:1.40E-5 ID:SGTC_813|Compound:k007-0158|FD-Score:-3.40|P-value:5.46E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.20|P-value:9.76E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:3.22|P-value:9.21E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:4.05|P-value:4.41E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:8.74|P-value:1.64E-17 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.22|P-value:9.98E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-5.20|P-value:2.88E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.67|P-value:2.16E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:7.66|P-value:6.80E-14 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.53|P-value:6.03E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:-3.27|P-value:8.44E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.27|P-value:7.77E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.76|P-value:1.38E-4 ID:SGTC_1635|Compound:st007035|FD-Score:5.01|P-value:6.55E-7 ID:SGTC_1658|Compound:st012944|FD-Score:-3.44|P-value:4.85E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.53|P-value:6.83E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.65|P-value:2.31E-4 ID:SGTC_1742|Compound:st038235|FD-Score:11.50|P-value:6.83E-29 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.76|P-value:1.55E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.35|P-value:4.97E-10 ID:SGTC_1800|Compound:st050079|FD-Score:-4.28|P-value:1.94E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-4.21|P-value:2.63E-5 ID:SGTC_1829|Compound:berberine|FD-Score:-4.30|P-value:1.78E-5 ID:SGTC_1941|Compound:st076221|FD-Score:-5.46|P-value:7.75E-8 ID:SGTC_1944|Compound:st076371|FD-Score:3.87|P-value:9.20E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.57|P-value:3.09E-4 ID:SGTC_2009|Compound:4012297|FD-Score:-3.74|P-value:1.63E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.84|P-value:1.00E-4 ID:SGTC_2078|Compound:5212016|FD-Score:3.28|P-value:7.53E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:-5.59|P-value:3.89E-8 ID:SGTC_2136|Compound:5328288|FD-Score:-6.29|P-value:7.13E-10 ID:SGTC_2237|Compound:6667747|FD-Score:-3.96|P-value:7.17E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.24|P-value:9.31E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.54|P-value:4.34E-8 ID:SGTC_2312|Compound:7706453|FD-Score:3.64|P-value:2.15E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-3.66|P-value:2.22E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.23|P-value:8.77E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.85|P-value:9.70E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.68|P-value:2.01E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.40|P-value:5.52E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-4.30|P-value:1.77E-5 ID:SGTC_2817|Compound:7995342|FD-Score:-3.94|P-value:7.68E-5 ID:SGTC_2824|Compound:7998404|FD-Score:-4.60|P-value:4.88E-6 ID:SGTC_2858|Compound:9025781|FD-Score:-3.25|P-value:9.17E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-5.23|P-value:2.53E-7 ID:SGTC_2900|Compound:5328528|FD-Score:3.31|P-value:6.68E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-4.84|P-value:1.67E-6 ID:SGTC_3009|Compound:9080819|FD-Score:4.23|P-value:2.14E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:-3.29|P-value:7.96E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:-3.84|P-value:1.13E-4 ID:SGTC_3090|Compound:9117696|FD-Score:3.63|P-value:2.26E-4 ID:SGTC_3096|Compound:9117468|FD-Score:3.40|P-value:5.05E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.23|P-value:2.19E-5 ID:SGTC_3240|Compound:9133527|FD-Score:3.50|P-value:3.53E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.46|P-value:4.45E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.01|P-value:5.83E-5 ID:SGTC_3277|Compound:9138724|FD-Score:4.36|P-value:1.27E-5 ID:SGTC_10|Compound:0279-0212|FD-Score:7.24|P-value:1.40E-12 ID:SGTC_19|Compound:0279-0214|FD-Score:5.31|P-value:1.47E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:6.69|P-value:5.20E-11 ID:SGTC_116|Compound:0155-0182|FD-Score:4.10|P-value:3.70E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.84|P-value:1.03E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:5.26|P-value:1.87E-7 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.27|P-value:7.76E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:5.33|P-value:1.29E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:4.07|P-value:4.06E-5 ID:SGTC_161|Compound:4065-0411|FD-Score:3.65|P-value:2.10E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:6.02|P-value:3.05E-9 ID:SGTC_173|Compound:1273-0061|FD-Score:12.70|P-value:6.83E-35 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.38|P-value:5.82E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:28.90|P-value:3.02E-171 ID:SGTC_275|Compound:trichlorophene|FD-Score:12.90|P-value:6.51E-36 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.69|P-value:1.97E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:6.17|P-value:1.24E-9 ID:SGTC_314|Compound:1671-0008|FD-Score:3.80|P-value:1.19E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.46|P-value:8.93E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:6.21|P-value:9.85E-10 ID:SGTC_431|Compound:k089-0097|FD-Score:-6.77|P-value:3.82E-11 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.86|P-value:9.49E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:4.01|P-value:5.30E-5 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.23|P-value:8.97E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:4.92|P-value:9.98E-7 ID:SGTC_602|Compound:3130-1533|FD-Score:4.01|P-value:5.27E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:4.92|P-value:9.77E-7 ID:SGTC_614|Compound:0462-0418|FD-Score:3.51|P-value:3.37E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.46|P-value:4.42E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-4.31|P-value:1.76E-5 ID:SGTC_767|Compound:0929-0023|FD-Score:4.33|P-value:1.40E-5 ID:SGTC_813|Compound:k007-0158|FD-Score:-3.40|P-value:5.46E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.20|P-value:9.76E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:3.22|P-value:9.21E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:4.05|P-value:4.41E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:8.74|P-value:1.64E-17 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.22|P-value:9.98E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-5.20|P-value:2.88E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.67|P-value:2.16E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:7.66|P-value:6.80E-14 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.53|P-value:6.03E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:-3.27|P-value:8.44E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.27|P-value:7.77E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.76|P-value:1.38E-4 ID:SGTC_1635|Compound:st007035|FD-Score:5.01|P-value:6.55E-7 ID:SGTC_1658|Compound:st012944|FD-Score:-3.44|P-value:4.85E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.53|P-value:6.83E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.65|P-value:2.31E-4 ID:SGTC_1742|Compound:st038235|FD-Score:11.50|P-value:6.83E-29 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.76|P-value:1.55E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-6.35|P-value:4.97E-10 ID:SGTC_1800|Compound:st050079|FD-Score:-4.28|P-value:1.94E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-4.21|P-value:2.63E-5 ID:SGTC_1829|Compound:berberine|FD-Score:-4.30|P-value:1.78E-5 ID:SGTC_1941|Compound:st076221|FD-Score:-5.46|P-value:7.75E-8 ID:SGTC_1944|Compound:st076371|FD-Score:3.87|P-value:9.20E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.57|P-value:3.09E-4 ID:SGTC_2009|Compound:4012297|FD-Score:-3.74|P-value:1.63E-4 ID:SGTC_2016|Compound:5121758|FD-Score:3.84|P-value:1.00E-4 ID:SGTC_2078|Compound:5212016|FD-Score:3.28|P-value:7.53E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:-5.59|P-value:3.89E-8 ID:SGTC_2136|Compound:5328288|FD-Score:-6.29|P-value:7.13E-10 ID:SGTC_2237|Compound:6667747|FD-Score:-3.96|P-value:7.17E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.24|P-value:9.31E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.54|P-value:4.34E-8 ID:SGTC_2312|Compound:7706453|FD-Score:3.64|P-value:2.15E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-3.66|P-value:2.22E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.23|P-value:8.77E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.85|P-value:9.70E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.68|P-value:2.01E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.40|P-value:5.52E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-4.30|P-value:1.77E-5 ID:SGTC_2817|Compound:7995342|FD-Score:-3.94|P-value:7.68E-5 ID:SGTC_2824|Compound:7998404|FD-Score:-4.60|P-value:4.88E-6 ID:SGTC_2858|Compound:9025781|FD-Score:-3.25|P-value:9.17E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-5.23|P-value:2.53E-7 ID:SGTC_2900|Compound:5328528|FD-Score:3.31|P-value:6.68E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-4.84|P-value:1.67E-6 ID:SGTC_3009|Compound:9080819|FD-Score:4.23|P-value:2.14E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:-3.29|P-value:7.96E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:-3.84|P-value:1.13E-4 ID:SGTC_3090|Compound:9117696|FD-Score:3.63|P-value:2.26E-4 ID:SGTC_3096|Compound:9117468|FD-Score:3.40|P-value:5.05E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.23|P-value:2.19E-5 ID:SGTC_3240|Compound:9133527|FD-Score:3.50|P-value:3.53E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.46|P-value:4.45E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.01|P-value:5.83E-5 ID:SGTC_3277|Compound:9138724|FD-Score:4.36|P-value:1.27E-5

Top fitness defect scores for YDR462W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 28.90 5.3 µM 3.02E-171 18 5.34E-184
2 SGTC_275 trichlorophene 12.90 5.7 µM 6.51E-36 mitochondrial stress 17 1.98E-38
3 SGTC_173 1273-0061 12.70 2.7 µM 6.83E-35 mitochondrial stress 19 2.48E-37
4 SGTC_1742 st038235 11.50 28.9 µM 6.83E-29 mitochondrial stress 7 6.96E-31
5 SGTC_908 3448-7491 8.74 47.7 µM 1.64E-17 mitochondrial stress 15 1.18E-18
6 SGTC_1188 1403-0014 7.66 4.6 µM 6.80E-14 mitochondrial stress 6 8.98E-15
7 SGTC_10 0279-0212 7.24 42.6 µM 1.40E-12 TRP & mitochondrial translation 12 2.31E-13
8 SGTC_70 0141-0289 6.69 43.3 µM 5.20E-11 mitochondrial stress 56 1.12E-11
9 SGTC_386 1509-0011 6.21 3.1 µM 9.85E-10 88 2.62E-10
10 SGTC_310 1273-0058 6.17 1.1 µM 1.24E-9 mitochondrial stress 139 3.35E-10
11 SGTC_168 3970-0862 6.02 530.5 µM 3.05E-9 41 8.79E-10
12 SGTC_2250 alverine citrate 5.54 130.0 µM 4.34E-8 70 1.51E-8
13 SGTC_134 0269-0018 5.33 6.9 µM 1.29E-7 92 4.86E-8
14 SGTC_19 0279-0214 5.31 38.4 µM 1.47E-7 TRP & mitochondrial translation 17 5.61E-8
15 SGTC_119 0121-0043 5.26 24.8 µM 1.87E-7 mitochondrial stress 45 7.23E-8
16 SGTC_1635 st007035 5.01 42.4 µM 6.55E-7 168 2.77E-7
17 SGTC_609 0986-0026 4.92 86.3 µM 9.77E-7 calcium & mitochondrial duress 11 4.26E-7
18 SGTC_596 1155-0152 4.92 116.0 µM 9.98E-7 TRP & mitochondrial translation 18 4.36E-7
19 SGTC_1203 3992-3522 4.53 103.0 µM 6.03E-6 15 2.99E-6
20 SGTC_3277 9138724 4.36 49.5 µM 1.27E-5 Golgi 18 6.62E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.668 0 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.636 0 YDR237W MRPL7 hom Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
0.630 0 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.626 0 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.603 0 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.602 0 YDR079W PET100 hom Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
0.600 0 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit
0.590 3.36E-313 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.582 2.94E-302 YMR098C ATP25 hom Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
0.582 5.38E-303 YDR533C HSP31 hom Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
0.579 5.55E-299 YGR102C GTF1 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
0.570 5.22E-288 YPR166C MRP2 hom Mitochondrial ribosomal protein of the small subunit
0.564 6.17E-281 YNL213C RRG9 hom Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.561 4.40E-276 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.550 4.00E-265 YGL064C MRH4 hom Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function