YDR464W / SPP41

Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR464W / SPP41

Click on Significant Values for Screen Details ID:SGTC_13|Compound:0199-0087|FD-Score:3.32|P-value:7.76E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:-3.25|P-value:6.61E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.57|P-value:3.23E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:4.52|P-value:7.37E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:3.37|P-value:6.47E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.03|P-value:5.81E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.70|P-value:1.33E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.39|P-value:6.15E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.96|P-value:7.57E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:-3.20|P-value:7.99E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:3.55|P-value:3.53E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:3.43|P-value:5.32E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:3.87|P-value:1.09E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.82|P-value:8.52E-5 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.40|P-value:4.04E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:4.03|P-value:5.66E-5 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.23|P-value:2.56E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:21.60|P-value:5.85E-97 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.44|P-value:3.49E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:9.98|P-value:2.87E-22 ID:SGTC_1130|Compound:3454-2858|FD-Score:-8.57|P-value:2.76E-17 ID:SGTC_1306|Compound:1185-0270|FD-Score:-3.21|P-value:7.68E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.69|P-value:2.10E-4 ID:SGTC_1606|Compound:st000094|FD-Score:-3.22|P-value:7.48E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.63|P-value:1.73E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.76|P-value:1.59E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.20|P-value:7.77E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.77|P-value:1.01E-4 ID:SGTC_2062|Compound:5245942|FD-Score:3.39|P-value:6.03E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:10.20|P-value:2.70E-23 ID:SGTC_2893|Compound:9048488|FD-Score:3.94|P-value:8.19E-5 ID:SGTC_2899|Compound:9053889|FD-Score:-3.90|P-value:6.11E-5 ID:SGTC_2924|Compound:9010168|FD-Score:3.74|P-value:1.74E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-9.34|P-value:3.83E-20 ID:SGTC_3085|Compound:9117376|FD-Score:4.14|P-value:3.69E-5 ID:SGTC_13|Compound:0199-0087|FD-Score:3.32|P-value:7.76E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:-3.25|P-value:6.61E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.57|P-value:3.23E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:4.52|P-value:7.37E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:3.37|P-value:6.47E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.03|P-value:5.81E-5 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.70|P-value:1.33E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.39|P-value:6.15E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.96|P-value:7.57E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:-3.20|P-value:7.99E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:3.55|P-value:3.53E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:3.43|P-value:5.32E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:3.87|P-value:1.09E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.82|P-value:8.52E-5 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.40|P-value:4.04E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:4.03|P-value:5.66E-5 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.23|P-value:2.56E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:21.60|P-value:5.85E-97 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.44|P-value:3.49E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:9.98|P-value:2.87E-22 ID:SGTC_1130|Compound:3454-2858|FD-Score:-8.57|P-value:2.76E-17 ID:SGTC_1306|Compound:1185-0270|FD-Score:-3.21|P-value:7.68E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.69|P-value:2.10E-4 ID:SGTC_1606|Compound:st000094|FD-Score:-3.22|P-value:7.48E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.63|P-value:1.73E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.76|P-value:1.59E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.20|P-value:7.77E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.77|P-value:1.01E-4 ID:SGTC_2062|Compound:5245942|FD-Score:3.39|P-value:6.03E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:10.20|P-value:2.70E-23 ID:SGTC_2893|Compound:9048488|FD-Score:3.94|P-value:8.19E-5 ID:SGTC_2899|Compound:9053889|FD-Score:-3.90|P-value:6.11E-5 ID:SGTC_2924|Compound:9010168|FD-Score:3.74|P-value:1.74E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-9.34|P-value:3.83E-20 ID:SGTC_3085|Compound:9117376|FD-Score:4.14|P-value:3.69E-5

Top fitness defect scores for YDR464W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1104 tacrolimus 21.60 29.8 µM 5.85E-97 2 2.59E-103 10.20
2 SGTC_2689 tacrolimus 10.20 100.0 µM 2.70E-23 2 7.32E-25 2.93
3 SGTC_1129 3453-1384 9.98 223.0 µM 2.87E-22 1 9.10E-24 3.15
4 SGTC_188 3229-0966 4.52 278.5 µM 7.37E-6 14 3.09E-6 0.09
5 SGTC_1005 4092-0690 4.23 4.4 µM 2.56E-5 plasma membrane duress 7 1.17E-5 0.11
6 SGTC_3085 9117376 4.14 49.5 µM 3.69E-5 6 1.73E-5 0.26
7 SGTC_995 3931-2021 4.03 34.6 µM 5.66E-5 RPP1 & pyrimidine depletion 5 2.74E-5 0.21
8 SGTC_367 0335-0881 4.03 7.1 µM 5.81E-5 ubiquinone biosynthesis & proteasome 22 2.82E-5 0.01
9 SGTC_612 0302-0167 3.96 22.4 µM 7.57E-5 copper-dependent oxidative stress 8 3.75E-5 0.38
10 SGTC_2893 9048488 3.94 26.0 µM 8.19E-5 fatty acid desaturase (OLE1) 10 4.08E-5 0.00
11 SGTC_838 0109-0203 3.87 71.4 µM 1.09E-4 3 5.52E-5 0.07
12 SGTC_1836 st055642 3.76 81.0 µM 1.59E-4 7 8.33E-5 0.46
13 SGTC_2924 9010168 3.74 62.8 µM 1.74E-4 cell wall signaling 7 9.15E-5 0.40
14 SGTC_1350 1495-0454 3.69 146.0 µM 2.10E-4 3 1.12E-4 0.12
15 SGTC_44 0929-0055 3.57 61.2 µM 3.23E-4 10 1.77E-4 0.02
16 SGTC_761 k216-0224 3.55 480.0 µM 3.53E-4 8 1.96E-4 0.16
17 SGTC_806 3966-4455 3.43 5.9 µM 5.32E-4 13 3.03E-4 0.06
18 SGTC_2062 5245942 3.39 200.0 µM 6.03E-4 2 3.47E-4 0.02
19 SGTC_596 1155-0152 3.39 116.0 µM 6.15E-4 TRP & mitochondrial translation 12 3.55E-4 0.08
20 SGTC_361 000s-0510 3.37 1.4 µM 6.47E-4 8 3.74E-4 0.22

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.430 5.97E-151 YLR029C RPL15A het Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
0.123 7.64E-13 YLL037W_d YLL037W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
0.115 2.80E-11 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.112 8.66E-11 YBR142W MAK5 het Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
0.111 1.03E-10 YMR226C YMR226C hom NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
0.111 1.32E-10 YDR245W MNN10 hom Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
0.100 6.70E-9 YLR342W FKS1 hom Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.097 1.98E-8 YGL111W NSA1 het Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
0.096 2.27E-8 YBL059W_p YBL059W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
0.095 3.11E-8 YHR079C-A SAE3 hom Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p
0.094 5.58E-8 YDL034W_d YDL034W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene
0.093 7.15E-8 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.093 7.49E-8 YLR142W PUT1 hom Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
0.092 8.39E-8 YHR080C YHR080C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication
0.091 1.12E-7 YDL195W SEC31 het Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance