YDR465C / RMT2

Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR465C / RMT2

Click on Significant Values for Screen Details ID:SGTC_80|Compound:0159-0065|FD-Score:-3.88|P-value:1.28E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:-3.29|P-value:9.35E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.63|P-value:8.83E-8 ID:SGTC_345|Compound:1362-0200|FD-Score:4.03|P-value:9.64E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.47|P-value:6.85E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.53|P-value:4.37E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:-3.34|P-value:8.13E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.88|P-value:1.65E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.86|P-value:1.38E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.56|P-value:5.09E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.44|P-value:5.89E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:-4.20|P-value:3.83E-5 ID:SGTC_1084|Compound:voriconazole|FD-Score:-3.48|P-value:5.14E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:-3.33|P-value:8.38E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:4.07|P-value:8.27E-5 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.42|P-value:6.21E-4 ID:SGTC_1634|Compound:st006576|FD-Score:-3.47|P-value:5.25E-4 ID:SGTC_1782|Compound:st039423|FD-Score:4.08|P-value:8.07E-5 ID:SGTC_1793|Compound:st048256|FD-Score:-3.32|P-value:8.76E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.51|P-value:1.50E-5 ID:SGTC_3313|Compound:9133968|FD-Score:-3.45|P-value:5.63E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.91|P-value:1.16E-4 ID:SGTC_80|Compound:0159-0065|FD-Score:-3.88|P-value:1.28E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:-3.29|P-value:9.35E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.63|P-value:8.83E-8 ID:SGTC_345|Compound:1362-0200|FD-Score:4.03|P-value:9.64E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.47|P-value:6.85E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.53|P-value:4.37E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:-3.34|P-value:8.13E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.88|P-value:1.65E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.86|P-value:1.38E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.56|P-value:5.09E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.44|P-value:5.89E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:-4.20|P-value:3.83E-5 ID:SGTC_1084|Compound:voriconazole|FD-Score:-3.48|P-value:5.14E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:-3.33|P-value:8.38E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:4.07|P-value:8.27E-5 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.42|P-value:6.21E-4 ID:SGTC_1634|Compound:st006576|FD-Score:-3.47|P-value:5.25E-4 ID:SGTC_1782|Compound:st039423|FD-Score:4.08|P-value:8.07E-5 ID:SGTC_1793|Compound:st048256|FD-Score:-3.32|P-value:8.76E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.51|P-value:1.50E-5 ID:SGTC_3313|Compound:9133968|FD-Score:-3.45|P-value:5.63E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.91|P-value:1.16E-4

Top fitness defect scores for YDR465C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 5.63 12.5 mM 8.83E-8 DNA intercalators 23 8.90E-9
2 SGTC_2293 7624732 4.51 200.0 µM 1.50E-5 16 3.26E-6
3 SGTC_1782 st039423 4.08 80.6 µM 8.07E-5 19 2.26E-5
4 SGTC_1123 mangostin 4.07 811.3 nM 8.27E-5 mitochondrial response to ROS 33 2.32E-5
5 SGTC_345 1362-0200 4.03 395.0 µM 9.64E-5 14 2.77E-5
6 SGTC_932 2857-0803 3.88 154.0 µM 1.65E-4 26 5.14E-5
7 SGTC_947 3447-0010 3.56 211.0 µM 5.09E-4 24 1.88E-4
8 SGTC_429 1112-0049 3.47 25.5 µM 6.85E-4 DNA damage response 56 2.64E-4
9 SGTC_1696 st025591 3.32 34.8 µM 0.00110 SWF1 & branched chain AA biosynthesis 47 4.54E-4
10 SGTC_1065 prochlorperazine maleate 3.19 2.5 µM 0.00161 37 7.04E-4
11 SGTC_1614 st002383 3.16 48.6 µM 0.00181 cell wall signaling 41 8.02E-4
12 SGTC_923 1900-2434 2.99 73.3 µM 0.00288 RNA processing & uracil transport 70 0.00137
13 SGTC_2299 7976368 2.98 114.9 µM 0.00297 68 0.00142
14 SGTC_1481 3346-2004 2.95 36.0 µM 0.00325 36 0.00157
15 SGTC_664 1222-0054 2.94 14.9 µM 0.00339 sphingolipid biosynthesis & PDR1 84 0.00165
16 SGTC_2762 leflunomide 2.92 42.1 µM 0.00355 86 0.00174
17 SGTC_1374 2889-5571 2.91 141.0 µM 0.00370 RSC complex & mRNA processing 102 0.00183
18 SGTC_860 0443-0016 2.91 25.1 µM 0.00370 70 0.00183
19 SGTC_854 0422-0053 2.90 192.0 µM 0.00374 PDR1 53 0.00185
20 SGTC_78 0336-0123 2.86 156.8 µM 0.00423 60 0.00213

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 1.84E-27 YPR082C DIB1 het 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
0.150 2.36E-18 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.143 1.11E-16 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.131 3.23E-14 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.127 1.65E-13 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.126 2.85E-13 YDL043C PRP11 het Subunit of the SF3a splicing factor complex, required for spliceosome assembly
0.125 4.16E-13 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.124 7.06E-13 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.123 9.52E-13 YDR113C PDS1 het Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
0.122 1.36E-12 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.117 1.36E-11 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.114 4.02E-11 YJL077W-B_p YJL077W-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.094 4.56E-8 YGR031W IMO32 hom Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
0.094 4.64E-8 YBR170C NPL4 hom Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
0.094 5.64E-8 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress