YDR471W / RPL27B

Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR471W / RPL27B

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.20|P-value:5.94E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.12|P-value:7.72E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:-3.75|P-value:7.47E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:3.56|P-value:1.56E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.74|P-value:7.88E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.76|P-value:7.26E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:4.49|P-value:2.67E-6 ID:SGTC_169|Compound:k015-0017|FD-Score:3.32|P-value:3.75E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.43|P-value:2.53E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.41|P-value:2.74E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-4.20|P-value:1.08E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.28|P-value:4.69E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.75|P-value:7.91E-7 ID:SGTC_517|Compound:1365-0151|FD-Score:3.41|P-value:2.72E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.18|P-value:6.31E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.23|P-value:5.21E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.80|P-value:5.82E-7 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.11|P-value:8.16E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-5.78|P-value:2.54E-9 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.30|P-value:4.05E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.53|P-value:1.82E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.18|P-value:6.64E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.85|P-value:4.71E-5 ID:SGTC_2061|Compound:5245884|FD-Score:3.14|P-value:7.32E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-3.82|P-value:5.76E-5 ID:SGTC_2312|Compound:7706453|FD-Score:-4.19|P-value:1.13E-5 ID:SGTC_2353|Compound:9059630|FD-Score:-3.18|P-value:6.55E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.08|P-value:8.90E-4 ID:SGTC_2362|Compound:9071156|FD-Score:4.02|P-value:2.36E-5 ID:SGTC_2487|Compound:5918373|FD-Score:-3.17|P-value:6.72E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.25|P-value:8.57E-6 ID:SGTC_2813|Compound:7971799|FD-Score:3.41|P-value:2.75E-4 ID:SGTC_2869|Compound:9037858|FD-Score:-3.19|P-value:6.47E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.52|P-value:1.79E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.20|P-value:5.94E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.12|P-value:7.72E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:-3.75|P-value:7.47E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:3.56|P-value:1.56E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.74|P-value:7.88E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.76|P-value:7.26E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:4.49|P-value:2.67E-6 ID:SGTC_169|Compound:k015-0017|FD-Score:3.32|P-value:3.75E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.43|P-value:2.53E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.41|P-value:2.74E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-4.20|P-value:1.08E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.28|P-value:4.69E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.75|P-value:7.91E-7 ID:SGTC_517|Compound:1365-0151|FD-Score:3.41|P-value:2.72E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.18|P-value:6.31E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.23|P-value:5.21E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:4.80|P-value:5.82E-7 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.11|P-value:8.16E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-5.78|P-value:2.54E-9 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.30|P-value:4.05E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-3.53|P-value:1.82E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.18|P-value:6.64E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.85|P-value:4.71E-5 ID:SGTC_2061|Compound:5245884|FD-Score:3.14|P-value:7.32E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-3.82|P-value:5.76E-5 ID:SGTC_2312|Compound:7706453|FD-Score:-4.19|P-value:1.13E-5 ID:SGTC_2353|Compound:9059630|FD-Score:-3.18|P-value:6.55E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.08|P-value:8.90E-4 ID:SGTC_2362|Compound:9071156|FD-Score:4.02|P-value:2.36E-5 ID:SGTC_2487|Compound:5918373|FD-Score:-3.17|P-value:6.72E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.25|P-value:8.57E-6 ID:SGTC_2813|Compound:7971799|FD-Score:3.41|P-value:2.75E-4 ID:SGTC_2869|Compound:9037858|FD-Score:-3.19|P-value:6.47E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.52|P-value:1.79E-4

Top fitness defect scores for YDR471W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1016 4226-1401 4.80 38.0 µM 5.82E-7 RNA pol III & RNase P/MRP 8 7.98E-7
2 SGTC_159 3970-0959 4.49 50.0 µM 2.67E-6 53 3.53E-6
3 SGTC_2362 9071156 4.02 126.0 µM 2.36E-5 8 2.97E-5
4 SGTC_1820 st053765 3.85 18.6 µM 4.71E-5 RPP1 & pyrimidine depletion 18 5.83E-5
5 SGTC_155 k072-0202 3.56 43.6 µM 1.56E-4 24 1.88E-4
6 SGTC_3054 9094397 3.52 49.5 µM 1.79E-4 fatty acid desaturase (OLE1) 46 2.15E-4
7 SGTC_374 0880-0231 3.43 82.3 µM 2.53E-4 iron homeostasis 42 3.01E-4
8 SGTC_517 1365-0151 3.41 113.0 µM 2.72E-4 50 3.23E-4
9 SGTC_474 dl-dihydrosphingosine 3.41 5.0 µM 2.74E-4 53 3.26E-4
10 SGTC_2813 7971799 3.41 71.4 µM 2.75E-4 39 3.26E-4
11 SGTC_169 k015-0017 3.32 16.0 µM 3.75E-4 35 4.42E-4
12 SGTC_1551 6-bromo-3'-methylflavone 3.30 63.5 µM 4.05E-4 43 4.76E-4
13 SGTC_920 mycophenolic acid 3.23 780.4 nM 5.21E-4 61 6.09E-4
14 SGTC_15 0266-0086 3.20 27.1 µM 5.94E-4 endomembrane recycling 105 6.93E-4
15 SGTC_557 1315-0110 3.18 16.2 µM 6.31E-4 Golgi 53 7.34E-4
16 SGTC_2061 5245884 3.14 81.0 µM 7.32E-4 tubulin folding & SWR complex 55 8.49E-4
17 SGTC_118 0083-0097 3.12 135.3 µM 7.72E-4 translation 243 8.95E-4
18 SGTC_1029 k015-0030 3.11 64.7 µM 8.16E-4 72 9.44E-4
19 SGTC_2359 9039622 3.08 200.0 µM 8.90E-4 endomembrane recycling 64 0.00103
20 SGTC_1127 asiatic acid 3.03 681.4 nM 0.00106 71 0.00122

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.55E-13 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.125 3.74E-13 YDR447C RPS17B hom Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.121 2.39E-12 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.114 3.11E-11 YMR310C_p YMR310C_p hom Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene
0.113 5.00E-11 YLR367W RPS22B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.101 4.79E-9 YDR500C RPL37B hom Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
0.101 4.88E-9 YDR024W_d FYV1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin
0.098 1.35E-8 YDL137W ARF2 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication
0.094 4.95E-8 YOR358W HAP5 hom Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
0.093 6.36E-8 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.093 6.54E-8 YDR048C_d YDR048C_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.093 7.87E-8 YDR446W ECM11 hom Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
0.093 7.92E-8 YER131W RPS26B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication
0.091 1.22E-7 YGR264C MES1 het Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
0.090 1.80E-7 YJR070C LIA1 hom Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress