YDR483W / KRE2

Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR483W / KRE2

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:3.98|P-value:3.64E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.23|P-value:1.61E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-8.73|P-value:2.44E-14 ID:SGTC_108|Compound:0509-0257|FD-Score:4.52|P-value:5.97E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:11.00|P-value:2.45E-21 ID:SGTC_118|Compound:0083-0097|FD-Score:-3.85|P-value:4.15E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.71|P-value:8.12E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:-3.59|P-value:8.97E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.09|P-value:2.51E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.98|P-value:3.60E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:7.61|P-value:3.98E-11 ID:SGTC_671|Compound:0560-0003|FD-Score:-3.62|P-value:8.29E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:4.04|P-value:2.96E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:-3.56|P-value:9.71E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.89|P-value:4.81E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:3.68|P-value:8.96E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.37|P-value:9.98E-5 ID:SGTC_1126|Compound:isoteolin|FD-Score:5.14|P-value:6.07E-6 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.28|P-value:1.39E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.00|P-value:3.41E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.33|P-value:2.85E-6 ID:SGTC_1360|Compound:1598-0022|FD-Score:5.16|P-value:5.54E-6 ID:SGTC_1887|Compound:st057648|FD-Score:4.25|P-value:1.53E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-4.20|P-value:1.34E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.81|P-value:4.65E-4 ID:SGTC_1958|Compound:st076513|FD-Score:-4.85|P-value:1.30E-5 ID:SGTC_1970|Compound:st070122|FD-Score:-5.22|P-value:3.10E-6 ID:SGTC_1973|Compound:st070275|FD-Score:4.39|P-value:9.39E-5 ID:SGTC_2190|Compound:6049624|FD-Score:4.27|P-value:1.43E-4 ID:SGTC_2206|Compound:7254556|FD-Score:4.42|P-value:8.65E-5 ID:SGTC_2217|Compound:6934799|FD-Score:4.20|P-value:1.80E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.96|P-value:3.83E-4 ID:SGTC_2367|Compound:9070478|FD-Score:5.36|P-value:2.53E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.41|P-value:8.73E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:7.11|P-value:6.45E-10 ID:SGTC_2952|Compound:9073731|FD-Score:4.45|P-value:7.72E-5 ID:SGTC_3025|Compound:9087895|FD-Score:3.72|P-value:7.92E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.92|P-value:4.31E-4 ID:SGTC_3311|Compound:9131146|FD-Score:6.31|P-value:3.67E-8 ID:SGTC_3321|Compound:9137793|FD-Score:11.20|P-value:3.93E-22 ID:SGTC_3322|Compound:9139058|FD-Score:5.36|P-value:2.44E-6 ID:SGTC_3350|Compound:9150499|FD-Score:4.42|P-value:8.67E-5 ID:SGTC_3351|Compound:9150312|FD-Score:5.47|P-value:1.57E-6 ID:SGTC_47|Compound:0149-0135|FD-Score:3.98|P-value:3.64E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.23|P-value:1.61E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-8.73|P-value:2.44E-14 ID:SGTC_108|Compound:0509-0257|FD-Score:4.52|P-value:5.97E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:11.00|P-value:2.45E-21 ID:SGTC_118|Compound:0083-0097|FD-Score:-3.85|P-value:4.15E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.71|P-value:8.12E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:-3.59|P-value:8.97E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.09|P-value:2.51E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.98|P-value:3.60E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:7.61|P-value:3.98E-11 ID:SGTC_671|Compound:0560-0003|FD-Score:-3.62|P-value:8.29E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:4.04|P-value:2.96E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:-3.56|P-value:9.71E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.89|P-value:4.81E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:3.68|P-value:8.96E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.37|P-value:9.98E-5 ID:SGTC_1126|Compound:isoteolin|FD-Score:5.14|P-value:6.07E-6 ID:SGTC_1172|Compound:0370-0043|FD-Score:4.28|P-value:1.39E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.00|P-value:3.41E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.33|P-value:2.85E-6 ID:SGTC_1360|Compound:1598-0022|FD-Score:5.16|P-value:5.54E-6 ID:SGTC_1887|Compound:st057648|FD-Score:4.25|P-value:1.53E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-4.20|P-value:1.34E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.81|P-value:4.65E-4 ID:SGTC_1958|Compound:st076513|FD-Score:-4.85|P-value:1.30E-5 ID:SGTC_1970|Compound:st070122|FD-Score:-5.22|P-value:3.10E-6 ID:SGTC_1973|Compound:st070275|FD-Score:4.39|P-value:9.39E-5 ID:SGTC_2190|Compound:6049624|FD-Score:4.27|P-value:1.43E-4 ID:SGTC_2206|Compound:7254556|FD-Score:4.42|P-value:8.65E-5 ID:SGTC_2217|Compound:6934799|FD-Score:4.20|P-value:1.80E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.96|P-value:3.83E-4 ID:SGTC_2367|Compound:9070478|FD-Score:5.36|P-value:2.53E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.41|P-value:8.73E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:7.11|P-value:6.45E-10 ID:SGTC_2952|Compound:9073731|FD-Score:4.45|P-value:7.72E-5 ID:SGTC_3025|Compound:9087895|FD-Score:3.72|P-value:7.92E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.92|P-value:4.31E-4 ID:SGTC_3311|Compound:9131146|FD-Score:6.31|P-value:3.67E-8 ID:SGTC_3321|Compound:9137793|FD-Score:11.20|P-value:3.93E-22 ID:SGTC_3322|Compound:9139058|FD-Score:5.36|P-value:2.44E-6 ID:SGTC_3350|Compound:9150499|FD-Score:4.42|P-value:8.67E-5 ID:SGTC_3351|Compound:9150312|FD-Score:5.47|P-value:1.57E-6

Top fitness defect scores for YDR483W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3321 9137793 11.20 26.8 µM 3.93E-22 cell wall 6 1.62E-29
2 SGTC_117 lucigenin 11.00 22.1 µM 2.45E-21 redox potentiating 6 1.94E-28
3 SGTC_514 4466-0038 7.61 2.6 nM 3.98E-11 cell wall 48 1.35E-14
4 SGTC_2584 androsterone 7.11 48.7 µM 6.45E-10 cell wall 37 5.88E-13
5 SGTC_3311 9131146 6.31 2.9 µM 3.67E-8 cell wall 56 1.40E-10
6 SGTC_3351 9150312 5.47 21.6 µM 1.57E-6 cell wall 13 2.24E-8
7 SGTC_3322 9139058 5.36 2.1 µM 2.44E-6 cell wall 11 4.07E-8
8 SGTC_2367 9070478 5.36 135.4 µM 2.53E-6 cell wall 15 4.27E-8
9 SGTC_1282 0886-0010 5.33 32.4 µM 2.85E-6 endomembrane recycling 16 5.02E-8
10 SGTC_1360 1598-0022 5.16 39.8 µM 5.54E-6 ERG2 14 1.23E-7
11 SGTC_1126 isoteolin 5.14 1.0 µM 6.07E-6 10 1.39E-7
12 SGTC_108 0509-0257 4.52 621.1 µM 5.97E-5 34 3.05E-6
13 SGTC_2952 9073731 4.45 13.8 µM 7.72E-5 tubulin folding & SWR complex 23 4.31E-6
14 SGTC_2206 7254556 4.42 147.0 µM 8.65E-5 60S ribosome export 1 5.03E-6
15 SGTC_3350 9150499 4.42 3.6 µM 8.67E-5 cell wall 36 5.04E-6
16 SGTC_2582 agaric acid 4.41 100.0 µM 8.73E-5 redox potentiating 268 5.09E-6
17 SGTC_1973 st070275 4.39 50.2 µM 9.39E-5 6 5.62E-6
18 SGTC_1029 k015-0030 4.37 64.7 µM 9.98E-5 19 6.10E-6
19 SGTC_1172 0370-0043 4.28 85.0 µM 1.39E-4 17 9.53E-6
20 SGTC_2190 6049624 4.27 200.0 µM 1.43E-4 3 9.93E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.357 1.43E-101 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.318 7.30E-80 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.303 5.83E-72 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.299 3.30E-70 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.281 9.23E-62 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.280 2.10E-61 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.277 2.84E-60 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.276 1.55E-59 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.276 1.50E-59 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.267 1.04E-55 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.264 1.82E-54 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.261 2.28E-53 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.261 2.37E-53 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.259 2.09E-52 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.239 8.09E-45 YNR040W_p YNR040W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies