HIPHOP chemogenomics database

YDR483W / KRE2

Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR483W / KRE2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR483W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_3321	9137793	11.20	26.8 µM	3.93E-22	cell wall	6	1.62E-29
2	SGTC_117	lucigenin	11.00	22.1 µM	2.45E-21	redox potentiating	6	1.94E-28
3	SGTC_514	4466-0038	7.61	2.6 nM	3.98E-11	cell wall	48	1.35E-14
4	SGTC_2584	androsterone	7.11	48.7 µM	6.45E-10	cell wall	37	5.88E-13
5	SGTC_3311	9131146	6.31	2.9 µM	3.67E-8	cell wall	56	1.40E-10
6	SGTC_3351	9150312	5.47	21.6 µM	1.57E-6	cell wall	13	2.24E-8
7	SGTC_3322	9139058	5.36	2.1 µM	2.44E-6	cell wall	11	4.07E-8
8	SGTC_2367	9070478	5.36	135.4 µM	2.53E-6	cell wall	15	4.27E-8
9	SGTC_1282	0886-0010	5.33	32.4 µM	2.85E-6	endomembrane recycling	16	5.02E-8
10	SGTC_1360	1598-0022	5.16	39.8 µM	5.54E-6	ERG2	14	1.23E-7
11	SGTC_1126	isoteolin	5.14	1.0 µM	6.07E-6		10	1.39E-7
12	SGTC_108	0509-0257	4.52	621.1 µM	5.97E-5		34	3.05E-6
13	SGTC_2952	9073731	4.45	13.8 µM	7.72E-5	tubulin folding & SWR complex	23	4.31E-6
14	SGTC_2206	7254556	4.42	147.0 µM	8.65E-5	60S ribosome export	1	5.03E-6
15	SGTC_3350	9150499	4.42	3.6 µM	8.67E-5	cell wall	36	5.04E-6
16	SGTC_2582	agaric acid	4.41	100.0 µM	8.73E-5	redox potentiating	268	5.09E-6
17	SGTC_1973	st070275	4.39	50.2 µM	9.39E-5		6	5.62E-6
18	SGTC_1029	k015-0030	4.37	64.7 µM	9.98E-5		19	6.10E-6
19	SGTC_1172	0370-0043	4.28	85.0 µM	1.39E-4		17	9.53E-6
20	SGTC_2190	6049624	4.27	200.0 µM	1.43E-4		3	9.93E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.357	1.43E-101	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.318	7.30E-80	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.303	5.83E-72	YMR124W_p	YMR124W_p	hom	Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.299	3.30E-70	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.281	9.23E-62	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.280	2.10E-61	YML117W	NAB6	hom	Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.277	2.84E-60	YBL006C	LDB7	hom	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.276	1.55E-59	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.276	1.50E-59	YDL066W	IDP1	hom	Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.267	1.04E-55	YJR142W_p	YJR142W_p	hom	Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.264	1.82E-54	YNL314W	DAL82	hom	Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.261	2.28E-53	YMR036C	MIH1	hom	Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.261	2.37E-53	YBR023C	CHS3	hom	Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.259	2.09E-52	YPL115C	BEM3	hom	Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.239	8.09E-45	YNR040W_p	YNR040W_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies