YDR486C / VPS60

Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR486C / VPS60

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:4.29|P-value:1.96E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.87|P-value:1.41E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.57|P-value:3.13E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.27|P-value:8.33E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.62|P-value:2.62E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.60|P-value:2.77E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.95|P-value:7.59E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.91|P-value:8.79E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.51|P-value:7.42E-6 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.88|P-value:9.62E-5 ID:SGTC_300|Compound:r073-0011|FD-Score:4.81|P-value:2.01E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.62|P-value:4.44E-6 ID:SGTC_308|Compound:4092-1169|FD-Score:3.67|P-value:2.18E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.26|P-value:2.07E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.53|P-value:6.68E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.35|P-value:6.40E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.09|P-value:4.29E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:-3.27|P-value:8.28E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:5.93|P-value:6.19E-9 ID:SGTC_527|Compound:4476-4215|FD-Score:-3.60|P-value:2.64E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:6.78|P-value:3.88E-11 ID:SGTC_683|Compound:panaxydol|FD-Score:-3.71|P-value:1.81E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:-3.57|P-value:2.96E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:5.66|P-value:2.76E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:6.39|P-value:4.43E-10 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.02|P-value:5.81E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:4.02|P-value:5.80E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.29|P-value:7.90E-10 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.75|P-value:1.60E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:4.36|P-value:1.47E-5 ID:SGTC_1658|Compound:st012944|FD-Score:4.32|P-value:1.74E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.34|P-value:6.62E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.88|P-value:9.79E-5 ID:SGTC_1934|Compound:st074720|FD-Score:-3.75|P-value:1.57E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.53|P-value:3.47E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.93|P-value:8.04E-5 ID:SGTC_2021|Compound:4019055|FD-Score:4.27|P-value:2.14E-5 ID:SGTC_2038|Compound:4100038|FD-Score:4.68|P-value:3.56E-6 ID:SGTC_2050|Compound:5263056|FD-Score:4.26|P-value:2.22E-5 ID:SGTC_2060|Compound:5238662|FD-Score:3.94|P-value:8.01E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.29|P-value:7.91E-10 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.50|P-value:7.54E-6 ID:SGTC_2322|Compound:7413319|FD-Score:10.20|P-value:5.06E-23 ID:SGTC_2334|Compound:9017922|FD-Score:4.26|P-value:2.16E-5 ID:SGTC_2338|Compound:7999974|FD-Score:4.35|P-value:1.53E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.29|P-value:8.00E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.62|P-value:2.61E-4 ID:SGTC_2700|Compound:st077718|FD-Score:-3.81|P-value:1.23E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.41|P-value:5.35E-4 ID:SGTC_2795|Compound:7780010|FD-Score:-3.26|P-value:8.56E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.56|P-value:6.01E-6 ID:SGTC_3197|Compound:9113331|FD-Score:3.69|P-value:1.97E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.32|P-value:6.96E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.29|P-value:1.96E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.87|P-value:1.41E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.57|P-value:3.13E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.27|P-value:8.33E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.62|P-value:2.62E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.60|P-value:2.77E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.95|P-value:7.59E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:3.91|P-value:8.79E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.51|P-value:7.42E-6 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.88|P-value:9.62E-5 ID:SGTC_300|Compound:r073-0011|FD-Score:4.81|P-value:2.01E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.62|P-value:4.44E-6 ID:SGTC_308|Compound:4092-1169|FD-Score:3.67|P-value:2.18E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.26|P-value:2.07E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.53|P-value:6.68E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.35|P-value:6.40E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.09|P-value:4.29E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:-3.27|P-value:8.28E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:5.93|P-value:6.19E-9 ID:SGTC_527|Compound:4476-4215|FD-Score:-3.60|P-value:2.64E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:6.78|P-value:3.88E-11 ID:SGTC_683|Compound:panaxydol|FD-Score:-3.71|P-value:1.81E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:-3.57|P-value:2.96E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:5.66|P-value:2.76E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:6.39|P-value:4.43E-10 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.02|P-value:5.81E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:4.02|P-value:5.80E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.29|P-value:7.90E-10 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.75|P-value:1.60E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:4.36|P-value:1.47E-5 ID:SGTC_1658|Compound:st012944|FD-Score:4.32|P-value:1.74E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.34|P-value:6.62E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.88|P-value:9.79E-5 ID:SGTC_1934|Compound:st074720|FD-Score:-3.75|P-value:1.57E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.53|P-value:3.47E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.93|P-value:8.04E-5 ID:SGTC_2021|Compound:4019055|FD-Score:4.27|P-value:2.14E-5 ID:SGTC_2038|Compound:4100038|FD-Score:4.68|P-value:3.56E-6 ID:SGTC_2050|Compound:5263056|FD-Score:4.26|P-value:2.22E-5 ID:SGTC_2060|Compound:5238662|FD-Score:3.94|P-value:8.01E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:6.29|P-value:7.91E-10 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.50|P-value:7.54E-6 ID:SGTC_2322|Compound:7413319|FD-Score:10.20|P-value:5.06E-23 ID:SGTC_2334|Compound:9017922|FD-Score:4.26|P-value:2.16E-5 ID:SGTC_2338|Compound:7999974|FD-Score:4.35|P-value:1.53E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.29|P-value:8.00E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.62|P-value:2.61E-4 ID:SGTC_2700|Compound:st077718|FD-Score:-3.81|P-value:1.23E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.41|P-value:5.35E-4 ID:SGTC_2795|Compound:7780010|FD-Score:-3.26|P-value:8.56E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.56|P-value:6.01E-6 ID:SGTC_3197|Compound:9113331|FD-Score:3.69|P-value:1.97E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.32|P-value:6.96E-4

Top fitness defect scores for YDR486C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2322 7413319 10.20 38.2 µM 5.06E-23 ERAD & cell cycle 18 8.01E-25
2 SGTC_568 1193-0097 6.78 7.5 µM 3.88E-11 ERAD & cell cycle 50 6.06E-12
3 SGTC_1060 3448-7350 6.39 82.0 µM 4.43E-10 Golgi 11 8.48E-11
4 SGTC_1495 4469-0029 6.29 237.0 µM 7.90E-10 45 1.59E-10
5 SGTC_2249 haloperidol 6.29 116.4 µM 7.91E-10 ergosterol depletion effects on membrane 39 1.59E-10
6 SGTC_525 k781-2675 5.93 26.7 µM 6.19E-9 redox potentiating 26 1.47E-9
7 SGTC_951 1171-0579 5.66 82.7 µM 2.76E-8 cell wall 37 7.45E-9
8 SGTC_300 r073-0011 4.81 5.0 µM 2.01E-6 7 7.73E-7
9 SGTC_2038 4100038 4.68 31.0 µM 3.56E-6 plasma membrane duress 29 1.43E-6
10 SGTC_2804 7954240 4.56 19.5 µM 6.01E-6 21 2.52E-6
11 SGTC_255 tunicamycin 4.51 200.5 nM 7.42E-6 unfolded protein response 53 3.17E-6
12 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 4.36 54.9 µM 1.47E-5 redox potentiating 45 6.62E-6
13 SGTC_2338 7999974 4.35 200.0 µM 1.53E-5 11 6.92E-6
14 SGTC_1658 st012944 4.32 20.0 µM 1.74E-5 iron homeostasis 67 7.97E-6
15 SGTC_37 0566-0070 4.29 1.6 µM 1.96E-5 19 9.04E-6
16 SGTC_2021 4019055 4.27 173.0 µM 2.14E-5 endomembrane recycling 104 9.97E-6
17 SGTC_2334 9017922 4.26 200.0 µM 2.16E-5 17 1.01E-5
18 SGTC_2050 5263056 4.26 171.0 µM 2.22E-5 fatty acid desaturase (OLE1) 35 1.04E-5
19 SGTC_351 caspofungin 4.09 25.0 nM 4.29E-5 cell wall 89 2.12E-5
20 SGTC_1183 1254-0324 4.02 198.0 µM 5.80E-5 20 2.93E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.253 3.87E-50 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.245 4.23E-47 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.234 6.68E-43 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.219 1.34E-37 YPR173C VPS4 hom AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.209 2.11E-34 YOR068C VAM10 hom Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
0.206 1.85E-33 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.199 3.51E-31 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.198 4.72E-31 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.198 4.94E-31 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.194 1.03E-29 YKR035W-A DID2 hom Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.190 1.69E-28 YKL002W DID4 hom Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.188 4.83E-28 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.185 3.45E-27 YNR005C_d YNR005C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184 5.12E-27 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.181 3.51E-26 YKR035C_d OPI8_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A