YDR487C / RIB3

3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR487C / RIB3

Click on Significant Values for Screen Details ID:SGTC_260|Compound:nsc-65238|FD-Score:-2.90|P-value:9.85E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.29|P-value:2.50E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-2.97|P-value:7.55E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.02|P-value:7.12E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-2.92|P-value:9.05E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.51|P-value:8.83E-7 ID:SGTC_859|Compound:0439-0102|FD-Score:3.22|P-value:3.29E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-6.39|P-value:5.00E-12 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.95|P-value:1.48E-5 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.55|P-value:8.80E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:-3.65|P-value:4.96E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.00|P-value:7.47E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.46|P-value:1.26E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-3.09|P-value:4.76E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.59|P-value:6.45E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.42|P-value:1.29E-4 ID:SGTC_1956|Compound:st077085|FD-Score:-3.65|P-value:5.00E-5 ID:SGTC_2280|Compound:7785178|FD-Score:-3.34|P-value:1.81E-4 ID:SGTC_2281|Compound:7943571|FD-Score:-3.46|P-value:1.13E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.43|P-value:1.23E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.23|P-value:3.18E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.26|P-value:2.47E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-3.49|P-value:9.94E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.08|P-value:7.97E-6 ID:SGTC_2794|Compound:7785382|FD-Score:3.31|P-value:2.31E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.01|P-value:6.45E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-3.00|P-value:6.83E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:-2.90|P-value:9.85E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.29|P-value:2.50E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-2.97|P-value:7.55E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.02|P-value:7.12E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-2.92|P-value:9.05E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.51|P-value:8.83E-7 ID:SGTC_859|Compound:0439-0102|FD-Score:3.22|P-value:3.29E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-6.39|P-value:5.00E-12 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.95|P-value:1.48E-5 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.55|P-value:8.80E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:-3.65|P-value:4.96E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.00|P-value:7.47E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.46|P-value:1.26E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-3.09|P-value:4.76E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.59|P-value:6.45E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.42|P-value:1.29E-4 ID:SGTC_1956|Compound:st077085|FD-Score:-3.65|P-value:5.00E-5 ID:SGTC_2280|Compound:7785178|FD-Score:-3.34|P-value:1.81E-4 ID:SGTC_2281|Compound:7943571|FD-Score:-3.46|P-value:1.13E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.43|P-value:1.23E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.23|P-value:3.18E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.26|P-value:2.47E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-3.49|P-value:9.94E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.08|P-value:7.97E-6 ID:SGTC_2794|Compound:7785382|FD-Score:3.31|P-value:2.31E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.01|P-value:6.45E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-3.00|P-value:6.83E-4

Top fitness defect scores for YDR487C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_807 1315-0151 4.51 48.0 µM 8.83E-7 6 3.21E-6 0.59
2 SGTC_2563 tomatine 4.08 8.5 µM 7.97E-6 13 2.29E-5 0.29
3 SGTC_1140 3229-1848 3.95 3.0 µM 1.48E-5 3 3.97E-5 0.01
4 SGTC_1149 3909-7961 3.55 23.3 µM 8.80E-5 9 1.96E-4 0.18
5 SGTC_1584 6-fluoro-dl-tryptophan 3.46 90.0 µM 1.26E-4 mitochondrial processes 7 2.69E-4 0.05
6 SGTC_2794 7785382 3.31 27.3 µM 2.31E-4 24 4.65E-4 0.09
7 SGTC_420 mitomycin C 3.29 81.9 mM 2.50E-4 9 4.99E-4 0.11
8 SGTC_2379 9035213 3.23 200.0 µM 3.18E-4 12 6.20E-4 0.18
9 SGTC_859 0439-0102 3.22 54.6 µM 3.29E-4 redox potentiating 19 6.39E-4 0.02
10 SGTC_491 tetrandrine 3.02 80.3 µM 7.12E-4 10 0.00127 0.05
11 SGTC_1313 1220-0047 3.00 237.0 µM 7.47E-4 15 0.00133 0.06
12 SGTC_1230 0170-0365 2.89 3.8 µM 0.00114 endomembrane recycling 35 0.00195 0.01
13 SGTC_1248 0469-0687 2.87 58.6 µM 0.00123 20 0.00208 0.01
14 SGTC_836 0330-0104 2.85 1.4 µM 0.00128 13 0.00216 0.06
15 SGTC_1932 st074806 2.82 40.9 µM 0.00144 25 0.00239 0.01
16 SGTC_2065 5221596 2.82 34.3 µM 0.00144 18 0.00240 0.02
17 SGTC_1224 0823-0416 2.75 294.0 µM 0.00186 13 0.00302 0.02
18 SGTC_1919 st067113 2.72 52.3 µM 0.00201 TSC3-RPN4 23 0.00324 0.03
19 SGTC_3327 9140648 2.67 26.9 µM 0.00243 50 0.00384 0.03
20 SGTC_2489 cantharidin 2.65 73.4 µM 0.00254 cell wall signaling 33 0.00400 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 2.09E-27 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.142 1.11E-16 YDL182W LYS20 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
0.142 1.14E-16 YIR016W_p YIR016W_p hom Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
0.140 3.17E-16 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
0.127 1.77E-13 YNL253W TEX1 hom Protein involved in mRNA export, component of the transcription export (TREX) complex
0.120 3.63E-12 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.117 1.19E-11 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.112 7.67E-11 YGL243W TAD1 hom tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala
0.112 8.23E-11 YHL046C PAU13 hom Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock
0.110 1.44E-10 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.109 2.47E-10 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.107 5.34E-10 YGR067C_p YGR067C_p hom Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p
0.107 5.65E-10 YDR501W PLM2 hom Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
0.103 2.04E-9 YHL025W SNF6 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions
0.100 7.10E-9 YNL020C ARK1 hom Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication