YDR489W / SLD5

Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR489W / SLD5

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.31|P-value:8.59E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:3.32|P-value:8.32E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.96|P-value:6.88E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.27|P-value:8.09E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.77|P-value:1.41E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.54|P-value:3.99E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.74|P-value:1.98E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.70|P-value:1.84E-4 ID:SGTC_460|Compound:trequinsin|FD-Score:3.71|P-value:2.20E-4 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:3.29|P-value:9.20E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.27|P-value:8.08E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.62|P-value:3.01E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:5.78|P-value:2.01E-8 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:4.05|P-value:6.25E-5 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.70|P-value:2.30E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.41|P-value:6.29E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-3.36|P-value:6.08E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-4.78|P-value:2.17E-6 ID:SGTC_1523|Compound:st012844|FD-Score:-3.94|P-value:7.44E-5 ID:SGTC_1540|Compound:guanosine|FD-Score:-3.88|P-value:9.34E-5 ID:SGTC_2072|Compound:5231554|FD-Score:3.43|P-value:5.81E-4 ID:SGTC_2280|Compound:7785178|FD-Score:3.90|P-value:1.08E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.79|P-value:1.31E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.61|P-value:3.15E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.52|P-value:4.23E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.80|P-value:2.64E-6 ID:SGTC_2987|Compound:9018338|FD-Score:3.64|P-value:2.80E-4 ID:SGTC_3157|Compound:9099274|FD-Score:-3.24|P-value:9.04E-4 ID:SGTC_3194|Compound:9111903|FD-Score:3.94|P-value:9.44E-5 ID:SGTC_3315|Compound:9136395|FD-Score:-3.67|P-value:2.01E-4 ID:SGTC_3334|Compound:9142812|FD-Score:3.47|P-value:5.16E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.31|P-value:8.59E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:3.32|P-value:8.32E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.96|P-value:6.88E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.27|P-value:8.09E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.77|P-value:1.41E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.54|P-value:3.99E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.74|P-value:1.98E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.70|P-value:1.84E-4 ID:SGTC_460|Compound:trequinsin|FD-Score:3.71|P-value:2.20E-4 ID:SGTC_461|Compound:N-acetylfarnesylcysteine|FD-Score:3.29|P-value:9.20E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:-3.27|P-value:8.08E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.62|P-value:3.01E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:5.78|P-value:2.01E-8 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:4.05|P-value:6.25E-5 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.70|P-value:2.30E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.41|P-value:6.29E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-3.36|P-value:6.08E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-4.78|P-value:2.17E-6 ID:SGTC_1523|Compound:st012844|FD-Score:-3.94|P-value:7.44E-5 ID:SGTC_1540|Compound:guanosine|FD-Score:-3.88|P-value:9.34E-5 ID:SGTC_2072|Compound:5231554|FD-Score:3.43|P-value:5.81E-4 ID:SGTC_2280|Compound:7785178|FD-Score:3.90|P-value:1.08E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.79|P-value:1.31E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.61|P-value:3.15E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.52|P-value:4.23E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.80|P-value:2.64E-6 ID:SGTC_2987|Compound:9018338|FD-Score:3.64|P-value:2.80E-4 ID:SGTC_3157|Compound:9099274|FD-Score:-3.24|P-value:9.04E-4 ID:SGTC_3194|Compound:9111903|FD-Score:3.94|P-value:9.44E-5 ID:SGTC_3315|Compound:9136395|FD-Score:-3.67|P-value:2.01E-4 ID:SGTC_3334|Compound:9142812|FD-Score:3.47|P-value:5.16E-4

Top fitness defect scores for YDR489W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_854 0422-0053 5.78 192.0 µM 2.01E-8 PDR1 1 3.79E-9 1.00
2 SGTC_2821 7996764 4.80 71.4 µM 2.64E-6 6 8.03E-7 0.41
3 SGTC_1065 prochlorperazine maleate 4.05 2.5 µM 6.25E-5 6 2.60E-5 0.15
4 SGTC_3194 9111903 3.94 49.5 µM 9.44E-5 5 4.09E-5 0.50
5 SGTC_2280 7785178 3.90 200.0 µM 1.08E-4 2 4.73E-5 0.41
6 SGTC_119 0121-0043 3.74 24.8 µM 1.98E-4 mitochondrial stress 29 9.22E-5 0.17
7 SGTC_460 trequinsin 3.71 123.0 µM 2.20E-4 2 1.03E-4 0.75
8 SGTC_1103 duloxetine 3.70 36.1 µM 2.30E-4 8 1.09E-4 0.02
9 SGTC_2987 9018338 3.64 71.4 µM 2.80E-4 RPP1 & pyrimidine depletion 8 1.35E-4 0.32
10 SGTC_574 1315-0397 3.62 40.0 µM 3.01E-4 15 1.46E-4 0.25
11 SGTC_2304 7443016 3.61 91.4 µM 3.15E-4 7 1.54E-4 0.03
12 SGTC_114 0364-0001 3.54 490.3 µM 3.99E-4 8 1.99E-4 0.03
13 SGTC_2663 amphotericin b 3.52 100.0 µM 4.23E-4 9 2.12E-4 0.01
14 SGTC_3334 9142812 3.47 21.9 µM 5.16E-4 8 2.64E-4 0.00
15 SGTC_2072 5231554 3.43 129.0 µM 5.81E-4 6 3.01E-4 0.06
16 SGTC_1205 4161-2785 3.41 76.0 µM 6.29E-4 5 3.28E-4 0.09
17 SGTC_22 0964-0034 3.32 14.5 µM 8.32E-4 unfolded protein response 36 4.46E-4 0.02
18 SGTC_6 0844-0013 3.31 19.8 µM 8.59E-4 21 4.62E-4 0.07
19 SGTC_461 N-acetylfarnesylcysteine 3.29 136.0 µM 9.20E-4 2 4.99E-4 0.52
20 SGTC_1821 st053465 3.26 65.6 µM 0.00100 8 5.49E-4 0.34

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.120 2.88E-12 YNL188W KAR1 het Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
0.107 5.79E-10 YML005W TRM12 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA
0.104 1.49E-9 YBR242W_p YBR242W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene
0.098 1.21E-8 YMR180C CTL1 hom RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm
0.092 8.33E-8 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.090 1.93E-7 YLR328W NMA1 hom Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
0.087 4.68E-7 YML003W_p YML003W_p hom Putative protein of unknown function
0.086 6.45E-7 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.082 2.11E-6 YHL024W RIM4 hom Putative RNA-binding protein required for the expression of early and middle sporulation genes
0.081 2.90E-6 YPL164C MLH3 hom Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
0.080 3.92E-6 YDR070C FMP16 hom Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
0.079 4.81E-6 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.078 6.65E-6 YEL041W YEF1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
0.077 8.05E-6 YEL016C NPP2 hom Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication
0.075 1.23E-5 YDR473C PRP3 het Splicing factor, component of the U4/U6-U5 snRNP complex