HIPHOP chemogenomics database

YDR490C / PKH1

Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR490C / PKH1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR490C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1799	st045822	6.09	46.1 µM	4.18E-11		1	5.82E-10
2	SGTC_2021	4019055	5.71	173.0 µM	5.47E-10	endomembrane recycling	46	5.61E-9
3	SGTC_1525	8-methoxypsoralen	5.37	92.5 µM	5.05E-9	DNA damage response	14	3.98E-8
4	SGTC_2343	7990716	4.89	14.8 µM	9.06E-8		8	5.07E-7
5	SGTC_1330	1469-0054	4.82	61.8 µM	1.31E-7	iron homeostasis	6	7.02E-7
6	SGTC_1844	rapamycin	4.23	4.0 nM	3.12E-6		143	1.15E-5
7	SGTC_1322	1348-1070	4.15	12.0 µM	4.81E-6		4	1.69E-5
8	SGTC_1370	2001-0092	4.04	44.6 µM	7.99E-6		31	2.65E-5
9	SGTC_1214	0358-0031	3.95	63.7 µM	1.22E-5		18	3.84E-5
10	SGTC_1820	st053765	3.83	18.6 µM	2.22E-5	RPP1 & pyrimidine depletion	19	6.54E-5
11	SGTC_2419	paclitaxel	3.75	166.5 µM	3.18E-5		51	8.98E-5
12	SGTC_2987	9018338	3.70	71.4 µM	3.93E-5	RPP1 & pyrimidine depletion	27	1.08E-4
13	SGTC_2688	kanamycin b	3.47	96.7 µM	1.05E-4	calcium & mitochondrial duress	446	2.58E-4
14	SGTC_2824	7998404	3.47	52.0 µM	1.07E-4		48	2.62E-4
15	SGTC_3147	9097027	3.41	49.5 µM	1.38E-4		44	3.30E-4
16	SGTC_1780	st038448	3.39	24.5 µM	1.47E-4	iron homeostasis	42	3.48E-4
17	SGTC_733	4069-0007	3.32	56.1 µM	1.98E-4	ERAD & cell cycle	117	4.52E-4
18	SGTC_547	1120-0019	3.16	610.0 nM	3.75E-4	azole & statin	59	7.97E-4
19	SGTC_734	2023-0417	3.13	80.0 µM	4.12E-4	ergosterol biosynthesis	49	8.66E-4
20	SGTC_1512	0259-0544	3.09	12.2 µM	4.81E-4		62	9.93E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.156	9.98E-20	YDR467C_d	YDR467C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.155	1.49E-19	YGL079W_p	KXD1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.145	3.26E-17	YGL203C	KEX1	hom	Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.139	8.13E-16	YKL061W_p	BLI1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.136	2.34E-15	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.130	4.92E-14	YCR075W-A_p	YCR075W-A_p	hom	Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.129	5.64E-14	YLR408C_p	BLS1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.124	6.71E-13	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.116	1.51E-11	YMR005W	TAF4	het	TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
0.116	1.73E-11	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.114	3.33E-11	YDL019C	OSH2	hom	Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.114	4.25E-11	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.110	1.69E-10	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.108	3.35E-10	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.107	5.96E-10	YGL211W	NCS6	hom	Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae