YDR490C / PKH1

Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR490C / PKH1

Click on Significant Values for Screen Details ID:SGTC_231|Compound:b-estradiol|FD-Score:3.07|P-value:5.25E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:3.16|P-value:3.75E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:-3.58|P-value:6.70E-5 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.74|P-value:3.33E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:3.32|P-value:1.98E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.13|P-value:4.12E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.95|P-value:1.22E-5 ID:SGTC_1322|Compound:1348-1070|FD-Score:4.15|P-value:4.81E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:4.82|P-value:1.31E-7 ID:SGTC_1341|Compound:1486-1360|FD-Score:-3.44|P-value:1.24E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:4.04|P-value:7.99E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:-4.48|P-value:8.97E-7 ID:SGTC_1377|Compound:2944-0135|FD-Score:3.01|P-value:6.52E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:-3.37|P-value:1.64E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.09|P-value:4.81E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:-3.00|P-value:7.06E-4 ID:SGTC_1519|Compound:esterone|FD-Score:-3.10|P-value:4.83E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:5.37|P-value:5.05E-9 ID:SGTC_1780|Compound:st038448|FD-Score:3.39|P-value:1.47E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.88|P-value:1.78E-5 ID:SGTC_1795|Compound:st049545|FD-Score:-3.51|P-value:9.05E-5 ID:SGTC_1799|Compound:st045822|FD-Score:6.09|P-value:4.18E-11 ID:SGTC_1800|Compound:st050079|FD-Score:2.96|P-value:7.93E-4 ID:SGTC_1801|Compound:st050089|FD-Score:-3.79|P-value:2.71E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.83|P-value:2.22E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.23|P-value:3.12E-6 ID:SGTC_1987|Compound:st072290|FD-Score:-3.36|P-value:1.69E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.71|P-value:5.47E-10 ID:SGTC_2343|Compound:7990716|FD-Score:4.89|P-value:9.06E-8 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.75|P-value:3.18E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.34|P-value:1.87E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.47|P-value:1.05E-4 ID:SGTC_2824|Compound:7998404|FD-Score:3.47|P-value:1.07E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.01|P-value:6.73E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.70|P-value:3.93E-5 ID:SGTC_3147|Compound:9097027|FD-Score:3.41|P-value:1.38E-4 ID:SGTC_3309|Compound:9129340|FD-Score:3.03|P-value:6.09E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:3.07|P-value:5.25E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:3.16|P-value:3.75E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:-3.58|P-value:6.70E-5 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.74|P-value:3.33E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:3.32|P-value:1.98E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.13|P-value:4.12E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.95|P-value:1.22E-5 ID:SGTC_1322|Compound:1348-1070|FD-Score:4.15|P-value:4.81E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:4.82|P-value:1.31E-7 ID:SGTC_1341|Compound:1486-1360|FD-Score:-3.44|P-value:1.24E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:4.04|P-value:7.99E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:-4.48|P-value:8.97E-7 ID:SGTC_1377|Compound:2944-0135|FD-Score:3.01|P-value:6.52E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:-3.37|P-value:1.64E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.09|P-value:4.81E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:-3.00|P-value:7.06E-4 ID:SGTC_1519|Compound:esterone|FD-Score:-3.10|P-value:4.83E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:5.37|P-value:5.05E-9 ID:SGTC_1780|Compound:st038448|FD-Score:3.39|P-value:1.47E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.88|P-value:1.78E-5 ID:SGTC_1795|Compound:st049545|FD-Score:-3.51|P-value:9.05E-5 ID:SGTC_1799|Compound:st045822|FD-Score:6.09|P-value:4.18E-11 ID:SGTC_1800|Compound:st050079|FD-Score:2.96|P-value:7.93E-4 ID:SGTC_1801|Compound:st050089|FD-Score:-3.79|P-value:2.71E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.83|P-value:2.22E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.23|P-value:3.12E-6 ID:SGTC_1987|Compound:st072290|FD-Score:-3.36|P-value:1.69E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.71|P-value:5.47E-10 ID:SGTC_2343|Compound:7990716|FD-Score:4.89|P-value:9.06E-8 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.75|P-value:3.18E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.34|P-value:1.87E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.47|P-value:1.05E-4 ID:SGTC_2824|Compound:7998404|FD-Score:3.47|P-value:1.07E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.01|P-value:6.73E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.70|P-value:3.93E-5 ID:SGTC_3147|Compound:9097027|FD-Score:3.41|P-value:1.38E-4 ID:SGTC_3309|Compound:9129340|FD-Score:3.03|P-value:6.09E-4

Top fitness defect scores for YDR490C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1799 st045822 6.09 46.1 µM 4.18E-11 1 5.82E-10
2 SGTC_2021 4019055 5.71 173.0 µM 5.47E-10 endomembrane recycling 46 5.61E-9
3 SGTC_1525 8-methoxypsoralen 5.37 92.5 µM 5.05E-9 DNA damage response 14 3.98E-8
4 SGTC_2343 7990716 4.89 14.8 µM 9.06E-8 8 5.07E-7
5 SGTC_1330 1469-0054 4.82 61.8 µM 1.31E-7 iron homeostasis 6 7.02E-7
6 SGTC_1844 rapamycin 4.23 4.0 nM 3.12E-6 143 1.15E-5
7 SGTC_1322 1348-1070 4.15 12.0 µM 4.81E-6 4 1.69E-5
8 SGTC_1370 2001-0092 4.04 44.6 µM 7.99E-6 31 2.65E-5
9 SGTC_1214 0358-0031 3.95 63.7 µM 1.22E-5 18 3.84E-5
10 SGTC_1820 st053765 3.83 18.6 µM 2.22E-5 RPP1 & pyrimidine depletion 19 6.54E-5
11 SGTC_2419 paclitaxel 3.75 166.5 µM 3.18E-5 51 8.98E-5
12 SGTC_2987 9018338 3.70 71.4 µM 3.93E-5 RPP1 & pyrimidine depletion 27 1.08E-4
13 SGTC_2688 kanamycin b 3.47 96.7 µM 1.05E-4 calcium & mitochondrial duress 446 2.58E-4
14 SGTC_2824 7998404 3.47 52.0 µM 1.07E-4 48 2.62E-4
15 SGTC_3147 9097027 3.41 49.5 µM 1.38E-4 44 3.30E-4
16 SGTC_1780 st038448 3.39 24.5 µM 1.47E-4 iron homeostasis 42 3.48E-4
17 SGTC_733 4069-0007 3.32 56.1 µM 1.98E-4 ERAD & cell cycle 117 4.52E-4
18 SGTC_547 1120-0019 3.16 610.0 nM 3.75E-4 azole & statin 59 7.97E-4
19 SGTC_734 2023-0417 3.13 80.0 µM 4.12E-4 ergosterol biosynthesis 49 8.66E-4
20 SGTC_1512 0259-0544 3.09 12.2 µM 4.81E-4 62 9.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.156 9.98E-20 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.155 1.49E-19 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.145 3.26E-17 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.139 8.13E-16 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.136 2.34E-15 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.130 4.92E-14 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.129 5.64E-14 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.124 6.71E-13 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.116 1.51E-11 YMR005W TAF4 het TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
0.116 1.73E-11 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.114 3.33E-11 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.114 4.25E-11 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.110 1.69E-10 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.108 3.35E-10 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.107 5.96E-10 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae