YDR493W / MZM1

Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR493W / MZM1

Click on Significant Values for Screen Details ID:SGTC_122|Compound:0236-0016|FD-Score:3.94|P-value:3.90E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:5.05|P-value:2.12E-7 ID:SGTC_205|Compound:1486-1330|FD-Score:3.46|P-value:2.56E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:6.15|P-value:3.80E-10 ID:SGTC_236|Compound:1509-0011|FD-Score:4.91|P-value:4.46E-7 ID:SGTC_329|Compound:k048-0121|FD-Score:3.17|P-value:7.16E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.62|P-value:1.37E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:4.06|P-value:2.34E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:5.38|P-value:3.57E-8 ID:SGTC_748|Compound:0136-0228|FD-Score:3.36|P-value:3.60E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.26|P-value:5.15E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:3.24|P-value:5.50E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:5.45|P-value:2.47E-8 ID:SGTC_871|Compound:0574-0004|FD-Score:4.66|P-value:1.50E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:4.03|P-value:2.65E-5 ID:SGTC_1002|Compound:4049-0206|FD-Score:7.86|P-value:2.17E-15 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.33|P-value:7.19E-6 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.53|P-value:2.64E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.27|P-value:6.67E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.49|P-value:2.23E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:4.69|P-value:1.28E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.71|P-value:9.82E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.71|P-value:9.60E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:5.34|P-value:4.39E-8 ID:SGTC_1413|Compound:3910-0535|FD-Score:5.79|P-value:3.44E-9 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.97|P-value:3.42E-5 ID:SGTC_1483|Compound:3453-0561|FD-Score:5.33|P-value:4.66E-8 ID:SGTC_1604|Compound:st002052|FD-Score:3.22|P-value:6.06E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.81|P-value:6.55E-5 ID:SGTC_1635|Compound:st007035|FD-Score:5.60|P-value:1.05E-8 ID:SGTC_1656|Compound:st012921|FD-Score:10.60|P-value:1.23E-26 ID:SGTC_1734|Compound:st037168|FD-Score:5.06|P-value:1.98E-7 ID:SGTC_1736|Compound:st036774|FD-Score:3.34|P-value:3.95E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.15|P-value:7.72E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.09|P-value:9.18E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.37|P-value:3.47E-4 ID:SGTC_1817|Compound:st052116|FD-Score:-3.36|P-value:4.88E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.84|P-value:5.77E-5 ID:SGTC_1970|Compound:st070122|FD-Score:3.20|P-value:6.48E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.92|P-value:4.15E-5 ID:SGTC_2108|Compound:5365811|FD-Score:3.72|P-value:9.17E-5 ID:SGTC_2124|Compound:5246320|FD-Score:4.32|P-value:7.23E-6 ID:SGTC_2127|Compound:5253358|FD-Score:3.56|P-value:1.73E-4 ID:SGTC_2172|Compound:5754775|FD-Score:4.41|P-value:4.99E-6 ID:SGTC_2275|Compound:7947463|FD-Score:5.31|P-value:5.33E-8 ID:SGTC_2283|Compound:7944793|FD-Score:4.06|P-value:2.33E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.51|P-value:2.12E-4 ID:SGTC_2296|Compound:7971656|FD-Score:7.13|P-value:5.20E-13 ID:SGTC_2316|Compound:5844804|FD-Score:3.89|P-value:4.74E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.37|P-value:3.50E-4 ID:SGTC_2328|Compound:6201408|FD-Score:4.82|P-value:6.96E-7 ID:SGTC_2366|Compound:9070441|FD-Score:3.09|P-value:9.34E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.84|P-value:5.66E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.61|P-value:1.45E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.26|P-value:5.27E-4 ID:SGTC_2899|Compound:9053889|FD-Score:3.45|P-value:2.66E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-4.31|P-value:1.13E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.10|P-value:8.88E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.08|P-value:9.64E-4 ID:SGTC_3128|Compound:9127691|FD-Score:3.22|P-value:5.89E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.94|P-value:3.90E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:5.05|P-value:2.12E-7 ID:SGTC_205|Compound:1486-1330|FD-Score:3.46|P-value:2.56E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:6.15|P-value:3.80E-10 ID:SGTC_236|Compound:1509-0011|FD-Score:4.91|P-value:4.46E-7 ID:SGTC_329|Compound:k048-0121|FD-Score:3.17|P-value:7.16E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.62|P-value:1.37E-4 ID:SGTC_549|Compound:1661-1316|FD-Score:4.06|P-value:2.34E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:5.38|P-value:3.57E-8 ID:SGTC_748|Compound:0136-0228|FD-Score:3.36|P-value:3.60E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.26|P-value:5.15E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:3.24|P-value:5.50E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:5.45|P-value:2.47E-8 ID:SGTC_871|Compound:0574-0004|FD-Score:4.66|P-value:1.50E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:4.03|P-value:2.65E-5 ID:SGTC_1002|Compound:4049-0206|FD-Score:7.86|P-value:2.17E-15 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.33|P-value:7.19E-6 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.53|P-value:2.64E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.27|P-value:6.67E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.49|P-value:2.23E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:4.69|P-value:1.28E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.71|P-value:9.82E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.71|P-value:9.60E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:5.34|P-value:4.39E-8 ID:SGTC_1413|Compound:3910-0535|FD-Score:5.79|P-value:3.44E-9 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.97|P-value:3.42E-5 ID:SGTC_1483|Compound:3453-0561|FD-Score:5.33|P-value:4.66E-8 ID:SGTC_1604|Compound:st002052|FD-Score:3.22|P-value:6.06E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.81|P-value:6.55E-5 ID:SGTC_1635|Compound:st007035|FD-Score:5.60|P-value:1.05E-8 ID:SGTC_1656|Compound:st012921|FD-Score:10.60|P-value:1.23E-26 ID:SGTC_1734|Compound:st037168|FD-Score:5.06|P-value:1.98E-7 ID:SGTC_1736|Compound:st036774|FD-Score:3.34|P-value:3.95E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.15|P-value:7.72E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.09|P-value:9.18E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.37|P-value:3.47E-4 ID:SGTC_1817|Compound:st052116|FD-Score:-3.36|P-value:4.88E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.84|P-value:5.77E-5 ID:SGTC_1970|Compound:st070122|FD-Score:3.20|P-value:6.48E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_2104|Compound:5389443|FD-Score:3.92|P-value:4.15E-5 ID:SGTC_2108|Compound:5365811|FD-Score:3.72|P-value:9.17E-5 ID:SGTC_2124|Compound:5246320|FD-Score:4.32|P-value:7.23E-6 ID:SGTC_2127|Compound:5253358|FD-Score:3.56|P-value:1.73E-4 ID:SGTC_2172|Compound:5754775|FD-Score:4.41|P-value:4.99E-6 ID:SGTC_2275|Compound:7947463|FD-Score:5.31|P-value:5.33E-8 ID:SGTC_2283|Compound:7944793|FD-Score:4.06|P-value:2.33E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.51|P-value:2.12E-4 ID:SGTC_2296|Compound:7971656|FD-Score:7.13|P-value:5.20E-13 ID:SGTC_2316|Compound:5844804|FD-Score:3.89|P-value:4.74E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.37|P-value:3.50E-4 ID:SGTC_2328|Compound:6201408|FD-Score:4.82|P-value:6.96E-7 ID:SGTC_2366|Compound:9070441|FD-Score:3.09|P-value:9.34E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.84|P-value:5.66E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.61|P-value:1.45E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.26|P-value:5.27E-4 ID:SGTC_2899|Compound:9053889|FD-Score:3.45|P-value:2.66E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-4.31|P-value:1.13E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.10|P-value:8.88E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.08|P-value:9.64E-4 ID:SGTC_3128|Compound:9127691|FD-Score:3.22|P-value:5.89E-4

Top fitness defect scores for YDR493W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1656 st012921 10.60 11.0 µM 1.23E-26 9 8.98E-27
2 SGTC_1002 4049-0206 7.86 172.0 µM 2.17E-15 1 1.99E-15
3 SGTC_2296 7971656 7.13 126.5 µM 5.20E-13 5 4.97E-13
4 SGTC_208 k072-0098 6.15 12.4 µM 3.80E-10 heme biosynthesis & mitochondrial translocase 12 3.81E-10
5 SGTC_1413 3910-0535 5.79 34.4 µM 3.44E-9 2 3.50E-9
6 SGTC_1635 st007035 5.60 42.4 µM 1.05E-8 143 1.08E-8
7 SGTC_833 3852-0053 5.45 558.0 µM 2.47E-8 4 2.54E-8
8 SGTC_646 4092-0839 5.38 27.3 µM 3.57E-8 plasma membrane duress 8 3.68E-8
9 SGTC_1337 1486-1011 5.34 40.3 µM 4.39E-8 2 4.54E-8
10 SGTC_1483 3453-0561 5.33 55.9 µM 4.66E-8 5 4.81E-8
11 SGTC_2275 7947463 5.31 200.0 µM 5.33E-8 iron homeostasis 18 5.51E-8
12 SGTC_1734 st037168 5.06 69.4 µM 1.98E-7 8 2.06E-7
13 SGTC_192 3455-0609 5.05 60.9 µM 2.12E-7 4 2.20E-7
14 SGTC_236 1509-0011 4.91 5.3 µM 4.46E-7 329 4.66E-7
15 SGTC_2328 6201408 4.82 200.0 µM 6.96E-7 10 7.30E-7
16 SGTC_1287 0971-0001 4.69 34.6 µM 1.28E-6 sphingolipid biosynthesis & PDR1 33 1.34E-6
17 SGTC_871 0574-0004 4.66 149.0 µM 1.50E-6 30 1.58E-6
18 SGTC_2172 5754775 4.41 163.9 µM 4.99E-6 11 5.27E-6
19 SGTC_1048 3161-0300 4.33 24.5 µM 7.19E-6 17 7.62E-6
20 SGTC_2124 5246320 4.32 200.0 µM 7.23E-6 heme biosynthesis & mitochondrial translocase 14 7.65E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 3.39E-26 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.167 2.28E-22 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.167 2.61E-22 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.163 1.81E-21 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.163 2.72E-21 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.161 6.18E-21 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.155 1.28E-19 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.152 8.61E-19 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.146 1.75E-17 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.146 2.20E-17 YKL087C CYT2 hom Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
0.145 2.81E-17 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.145 2.81E-17 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.145 3.41E-17 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.143 9.14E-17 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.142 1.63E-16 YER058W PET117 hom Protein required for assembly of cytochrome c oxidase